Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F34 I40 R1 11 55.0 1783796 96.5% 1721363 148.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,719,4260TC100.0% 198.7 / NA 65intergenic (‑321/+2)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/26);  total (39/26)

TGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719285‑2719566
                                                                                                                                             |                                                                                                                                             
tGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCt                                                                                                                                        <  1:841341/149‑1 (MQ=255)
tGGAAGTGGGCAAGTTGGGGAAGCCATATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCt                                                                                                                                        >  2:324852/1‑149 (MQ=255)
  gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCCgg                                                                                                                                      >  1:343177/1‑149 (MQ=255)
           aaGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCt                                                                                                                             <  2:465343/149‑1 (MQ=255)
            aGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCACAAAGGCCATCCGTCAGGATGGCCTCTCGCATAATTCGATGCCTGGCAGTCCCCtc                                                                                                                            >  1:350208/1‑148 (MQ=255)
            aGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCGGGATGGCCTTTCGCATCATTCGATGCCTGGCAGTTcccga                                                                                                                            >  1:400548/1‑147 (MQ=255)
            aGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTGCCTa                                                                                                                            >  1:890491/1‑149 (MQ=255)
              ttGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTAct                                                                                                                          >  2:157401/1‑149 (MQ=255)
                  ggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCg                                                                                                                      >  2:304522/1‑149 (MQ=255)
                   gAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                     >  2:148348/1‑149 (MQ=255)
                   gAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                     >  2:27287/1‑149 (MQ=255)
                      gCCGTATCCGTTGCTGAATCTGGTATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                  <  2:661480/149‑1 (MQ=255)
                      gCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                  >  2:351044/1‑149 (MQ=255)
                      gCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                  <  1:884577/149‑1 (MQ=255)
                        cGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGCTGGCCTTGCGCATAATTCGATGCCTGGCAGTTCCCCACTCTCGCATggg                                                                                                                <  2:639685/149‑1 (MQ=255)
                        cGTACCCGTTGCTGAATCTGGCATATGTGGGAGGATACGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATggg                                                                                                                <  2:304358/149‑1 (MQ=255)
                               gTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAccc                                                                                                         >  2:840441/1‑149 (MQ=255)
                                   ctgcatacgcgacatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                     >  1:233831/14‑149 (MQ=25)
                                   cTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                     >  1:631916/1‑149 (MQ=255)
                                    taaaTCTGGCATATGTGAGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                                                                    <  2:177697/147‑1 (MQ=255)
                                       aTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAc                                                                                                 >  2:603031/1‑149 (MQ=255)
                                        tCTGGCATATGTGGGAGTATAAGAAGCGGAGGGTCGAATCTGGCTTTGTTTGAGGCTTGAATGCAAAAGGGCGATCGTTCATGATGGCGCTTCGCTTAATTCGAAGCCTGGCAGTTGCCTACTCTCGCATGGGGGGACCCCACaccacc                                                                                                >  1:178988/1‑149 (MQ=255)
                                           gggctatGTGGGGGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCTGGCTGGCCTTTCGCATATTTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACCCTACCATc                                                                                             <  1:351068/145‑1 (MQ=25)
                                           ggCATATGTGGGAGTATAAGCCGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATc                                                                                             <  1:166409/149‑1 (MQ=255)
                                             cATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCgg                                                                                           <  1:775848/149‑1 (MQ=255)
                                                  gtgGGCGTATAAGACGCGCAGCGGCGCATCAGGCATTTTTCTCTGCCCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAAGTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTa                                                                                      <  1:262122/149‑1 (MQ=25)
                                                  gtgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGACAATGCAAAAATGCCATCCGACAGGCTGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGGGACACCACACTACCCTCGGCGCTa                                                                                      >  1:399888/1‑149 (MQ=37)
                                                  gtgGGAGGATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTa                                                                                      >  1:841789/1‑149 (MQ=255)
                                                   tgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCACGATTGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCCTCCGCGCTAc                                                                                     >  1:412894/1‑149 (MQ=25)
                                                    gggAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGGCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGCGACCCCACACTACCCTCGGCGCTACg                                                                                    >  1:357464/1‑149 (MQ=255)
                                                       aGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCg                                                                                 >  1:516390/1‑149 (MQ=255)
                                                          ataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                              >  2:620099/1‑149 (MQ=255)
                                                            aaGCCGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCACTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                                                            <  1:557714/149‑1 (MQ=255)
                                                            aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGCCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTGCCCTAGCCTCGCATGGGGAGTCCACACAGTACCATCGGCGCTACGGCGTTTCa                                                                            >  2:74982/1‑149 (MQ=21)
                                                             aGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTAAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCGACGCTACCGTCGGCGCTACGGCGGTTCAc                                                                           >  2:272168/1‑149 (MQ=21)
                                                              gACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGCCCCCCCACTACCATCGCCGCTCCGCCGTTTCACt                                                                          >  1:406411/1‑149 (MQ=25)
                                                               aCGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTACGGCGTTTCACtt                                                                         >  1:74080/1‑149 (MQ=255)
                                                                   gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                     >  2:724790/1‑149 (MQ=255)
                                                                              tCAGGCATTTTTTTCGGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑gg                                                         <  2:426683/149‑1 (MQ=255)
                                                                               cAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGTTAT‑ggg                                                        <  1:241319/149‑1 (MQ=255)
                                                                               cAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGAGGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑ggg                                                        <  1:17163/149‑1 (MQ=255)
                                                                                     tttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAgg                                                  >  1:823204/1‑149 (MQ=255)
                                                                                     tttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTCCACTTCTGAGTTCGGCAT‑GGGGTCAgg                                                  >  1:241615/1‑149 (MQ=255)
                                                                                      ttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGt                                                 <  1:157401/149‑1 (MQ=255)
                                                                                        ttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTgg                                               <  1:27287/149‑1 (MQ=255)
                                                                                                gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACcg                                       <  1:603031/149‑1 (MQ=255)
                                                                                                gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGGCAGGTGGGACCACcg                                       >  2:714742/1‑149 (MQ=255)
                                                                                                   aaTGCAGAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCCACGCAGGTTCACTCCTGAGCTCGGCCTGGGGGTCAAGTGGGcccgccccc                                     >  1:22543/1‑146 (MQ=17)
                                                                                                        aaaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACgg                               >  2:858338/1‑149 (MQ=255)
                                                                                                        aaaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGAGTTTGACTTCTTAGTTCGGCAT‑GGGGTCAGTTGGGACCACCGCGCTACgg                               >  1:757597/1‑149 (MQ=17)
                                                                                                             ggCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGGCCACCGAGCTACGtccgcc                          >  1:749007/1‑149 (MQ=255)
                                                                                                               ccATCCGCCAGGAGGGCCTGTCGCATAACTCGATGCCGGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                        <  2:441762/149‑1 (MQ=14)
                                                                                                                 accggTCAGGATGGCCTTTCGCCTAAGTCGATGCCTGGCAGTTCCCTACTCTTGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGc                      <  1:556410/145‑1 (MQ=14)
                                                                                                                         ggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTcag              >  2:289539/1‑147 (MQ=255)
                                                                                                                         ggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTg              <  2:51549/149‑1 (MQ=255)
                                                                                                                         ggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTg              >  1:228866/1‑149 (MQ=255)
                                                                                                                          gATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt             <  2:418076/149‑1 (MQ=255)
                                                                                                                              gCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTa         <  2:516390/149‑1 (MQ=38)
                                                                                                                               ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        >  2:440281/1‑149 (MQ=37)
                                                                                                                               ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCAGAGTTCGGCAT‑GGGGTCAGGTGCGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        <  1:546778/149‑1 (MQ=255)
                                                                                                                                cTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATc       >  2:524324/1‑149 (MQ=25)
                                                                                                                                 tttCGCATAATTCGATGCCTGGCAGTTCCCGACTCTCGCATGGGGAGACACCACACTACCATCGGCGCTACGGCGTTTCTCTTCTGAGTTCGGCAT‑GGGGTCAGGGGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGCTTTATCa      >  2:221351/1‑149 (MQ=11)
                                                                                                                                   tCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGa    <  2:233831/149‑1 (MQ=18)
                                                                                                                                   ccGCATAATTCGATGCCTGGCATTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGa    <  1:809095/148‑1 (MQ=17)
                                                                                                                                     gCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCAGCGCTACGCCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTCCGGCCGCCAGGCAAATGCCGTTTTATCATAcc  >  1:327879/1‑149 (MQ=11)
                                                                                                                                             |                                                                                                                                             
TGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719285‑2719566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A