Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F20 I0 R1	92	76.5	1681216	93.1%	1565212	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	478,502	C→A	100%	D342E (GAC→GAA)	ndhF →	NADH dehydrogenase (ubiquinone)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	478,502	0	C	A	99.0%	291.7 / ‑5.9	104	D342E (GAC→GAA)	ndhF	NADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (47/53); total (48/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 104 total aligned reads displayed.

GTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA  >  CP000730/478365‑478639

gTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                        >  2:757050/1‑141 (MQ=255)

    aaTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATg                                                                                                                                    >  1:381994/1‑141 (MQ=255)

     aTGTGCATTGGGTGTGTATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATgg                                                                                                                                   >  1:622014/1‑141 (MQ=255)

     aTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCCTATgg                                                                                                                                   >  2:752897/1‑141 (MQ=255)

         gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTgg                                                                                                                               <  2:424292/141‑1 (MQ=255)

            ttcggTGGAGATGCAGCAGTGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGTATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                            <  1:453480/138‑1 (MQ=255)

            ttGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                            >  1:176654/1‑141 (MQ=255)

            ttGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                            >  2:796726/1‑141 (MQ=255)

             tGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTg                                                                                                                           <  1:167930/141‑1 (MQ=255)

             tGGGTGTAAATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTg                                                                                                                           <  1:479595/141‑1 (MQ=255)

                gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                        <  2:304574/141‑1 (MQ=255)

                  gtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATg                                                                                                                      >  1:807009/1‑141 (MQ=255)

                       ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGACAGCTATGGCTGGATTGTCATGGGACg                                                                                                                 <  2:594294/141‑1 (MQ=255)

                       ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                 >  1:153064/1‑141 (MQ=255)

                       ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                 >  1:347106/1‑141 (MQ=255)

                        tCAGCTGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  2:77534/141‑1 (MQ=255)

                        tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAATGGCGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  2:384689/141‑1 (MQ=255)

                        tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  1:57285/141‑1 (MQ=255)

                        tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  2:202454/141‑1 (MQ=255)

                        tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  2:439774/141‑1 (MQ=255)

                         cagcagCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTGATGGCTGGATTGTCATGGGACggg                                                                                                               >  1:664231/1‑139 (MQ=255)

                                tactaggcATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATACTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                        <  2:411057/134‑1 (MQ=255)

                                 attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGACTGTCATGGGACGTGTATTAGCt                                                                                                       >  2:691027/1‑141 (MQ=255)

                                  ttattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTGTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                      >  1:442545/1‑141 (MQ=255)

                                   tattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat                                                                                                     >  1:743531/1‑141 (MQ=255)

                                     ttCATTTAATATTGCACGGTATTTTTTTTTTTTTTTTTTTTTTAAAATGGGGTTCTATCGTGGTGCG‑ATTTATTTTTGCAATACAAGGATCTGCTTCAGAAGCTTATGGCTGGTTTGTCTTGGGTCGTTTAttggtttttt                                                                                                   >  2:419291/1‑133 (MQ=255)

                                         tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGt                                                                                               >  1:688628/1‑141 (MQ=255)

                                         tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGt                                                                                               <  1:329500/141‑1 (MQ=255)

                                              tatTGCACGGTATTCTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGTTGGATTGTCATGGGACGTGTATTAGCTATTATCGTTGCAt                                                                                          <  1:598591/141‑1 (MQ=255)

                                               atTGCACGGTTTTTTTAAAGCAAGATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGTCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                         <  2:706003/141‑1 (MQ=255)

                                               atTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                         >  1:192084/1‑141 (MQ=255)

                                                ttGCACGGTATTTTTAAAGCACCATTATTTTTTGTAGCAGGTTCTGGTAT‑CGGCGTAGTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAttt                                                                                        <  1:190319/141‑1 (MQ=255)

                                                  gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                      >  2:792261/1‑141 (MQ=255)

                                                  gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCCATTATCGTGGCGTTTCt                                                                                      >  2:792316/1‑141 (MQ=255)

                                                  gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTAACTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTTTTAGCTATTGTCTTGGCATTTCt                                                                                      >  2:380742/1‑141 (MQ=255)

                                                    aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAt                                                                                    <  2:459300/141‑1 (MQ=255)

                                                     cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTggtctttgcatt                                                                                   >  2:367373/1‑131 (MQ=255)

                                                     cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtt                                                                                   >  2:35361/1‑141 (MQ=255)

                                                     cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtt                                                                                   >  2:389302/1‑141 (MQ=255)

                                                         ttttctAAAGCAATATTATTTTTATAATCAGGTTCTATCGTGAAGCG‑ATTTAGTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                               <  2:352719/140‑1 (MQ=255)

                                                         aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                               >  2:566564/1‑141 (MQ=255)

                                                              tAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGag                                                                          <  1:32828/141‑1 (MQ=255)

                                                                ttttttttttttttttACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGAATCTGCTAAAGGAGCTTTTGGCTTGATTGTTATGTGATGTGTAATAGCTATTTTCGTGGCATTTCTATTTGTGATGagta                                                                        >  2:748138/12‑141 (MQ=255)

                                                                aaGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagta                                                                        >  2:178329/1‑141 (MQ=255)

                                                                    aaCATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa                                                                    <  2:711580/141‑1 (MQ=255)

                                                                    aaCATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa                                                                    <  1:124149/141‑1 (MQ=255)

                                                                     aCATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCGTTTCTATTTTGGTTGAGTAGTGAc                                                                   >  1:340289/1‑141 (MQ=255)

                                                                     aCATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGAc                                                                   >  1:536217/1‑141 (MQ=255)

                                                                      cATTATTTTTACAATCAGGTTCAATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACa                                                                  <  1:835990/141‑1 (MQ=255)

                                                                       attattTTTACAATCAGGTTATATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGATGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTAGCGTGGCATTTCTATTTTGGATGAGTAGTGACAg                                                                 <  1:250060/141‑1 (MQ=255)

                                                                         tattTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGGTGAGTAGTGACAGaa                                                               >  2:50580/1‑141 (MQ=255)

                                                                            ttttACAATCAGGTTCTATCGTGAAGCG‑ATTCACTATTCCAAAACACGCATCTGCTAAAGAAGCTTATGGCTGGATTGTACTGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGCTAAGTAGTGCCAGAAGTg                                                            >  2:649148/1‑141 (MQ=255)

                                                                            ttttACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTg                                                            >  2:832507/1‑141 (MQ=255)

                                                                            ttttACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTg                                                            <  1:683264/141‑1 (MQ=255)

                                                                             tttACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                           >  1:59585/1‑141 (MQ=255)

                                                                              ttACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGATGGATTGTCATGGGACGTGTATTAGCAATTATCGTGGCAGTTCTATTTTGGGTGAGTAGTGACAGAAGTGCa                                                          >  2:417286/1‑141 (MQ=255)

                                                                               tACAATGAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCat                                                         <  1:309085/141‑1 (MQ=255)

                                                                                atactCAGGTTCTATCGTGAAGCG‑ATTTAATATTCCAAAACAAGCATCTGCGAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAGTTCTATTTTGGATGAGTAGTGACAGAAGTGCata                                                        <  1:657332/137‑1 (MQ=255)

                                                                                   aTCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGGTGAGTAGTGACAGAAGTGCATATGa                                                     >  2:446840/1‑141 (MQ=255)

                                                                                    tCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGaa                                                    <  1:595885/141‑1 (MQ=255)

                                                                                     cAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                   >  1:258333/1‑141 (MQ=255)

                                                                                     cAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                   >  2:818358/1‑141 (MQ=255)

                                                                                      aGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgg                                                  >  2:243706/1‑140 (MQ=255)

                                                                                        gTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtgt                                                <  2:661501/141‑1 (MQ=255)

                                                                                          tCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTa                                              <  1:796726/141‑1 (MQ=255)

                                                                                             ttCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                           <  1:275309/140‑1 (MQ=255)

                                                                                             aTCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                           >  1:30611/1‑141 (MQ=255)

                                                                                              tCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTg                                          <  2:802884/141‑1 (MQ=255)

                                                                                                 tGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGGGACAGAAGCGCATATGAAGGTTTAAGTGCAc                                       >  1:284191/1‑141 (MQ=255)

                                                                                                  gAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt                                      <  2:622014/141‑1 (MQ=255)

                                                                                                     gCG‑ATTGAATATTCCAAAACAAGCATCTGCTAAAGAAGCTGATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                   <  1:792316/141‑1 (MQ=255)

                                                                                                     gCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                   >  2:784223/1‑141 (MQ=255)

                                                                                                     gCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                   <  1:792261/141‑1 (MQ=255)

                                                                                                     gCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                   <  1:121449/141‑1 (MQ=255)

                                                                                                       g‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTc                                 >  1:452580/1‑141 (MQ=255)

                                                                                                         aTTCAATATTCCTAAAACAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCt                                <  1:396627/141‑1 (MQ=255)

                                                                                                         aTTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCt                                >  2:737799/1‑141 (MQ=255)

                                                                                                            cAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTCACAGTAGTAGGTACAGCAGTTCATATGAAGTGTTAAGTGCACTCATTCTAgg                             <  2:381994/141‑1 (MQ=255)

                                                                                                             aaTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGa                            <  1:35361/141‑1 (MQ=255)

                                                                                                             aaTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGGAGTTACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGa                            <  1:367373/141‑1 (MQ=255)

                                                                                                               tatTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATg                          <  1:26380/141‑1 (MQ=255)

                                                                                                                atTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATgg                         <  1:691027/141‑1 (MQ=255)

                                                                                                                   ccAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCATGGCATTTCTATTTTGGATGAGTCGTGACCCAAGTTCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCa                      >  1:154986/1‑141 (MQ=255)

                                                                                                                      aaaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                   <  2:638011/141‑1 (MQ=255)

                                                                                                                      aaaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                   <  2:229665/141‑1 (MQ=255)

                                                                                                                       atctagccacTGCTAAAGCAGCTTATGGCTGGTTTGTCATGGGAGGTGTATTAGCTATTATCGTGGCATTTCTTTTTTGGATGTGTAGTGACAGAAGTGCATATGAACTGTTAAGTGCTCTCATTCTAGGATGGTCATTAc                  <  2:220768/132‑1 (MQ=255)

                                                                                                                       aatatggtagcgCTAAAGAATCTTATGGTGGGATTGTCATGGGACGTGTATTAGCTATTAGTGTGGGATTTCTATTTTGGATGTGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTAc                  <  2:190177/130‑1 (MQ=255)

                                                                                                                        aCAAGCATCTGCTAAAGAAGCTTATGGCGGGATTGGCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACt                 <  1:99352/141‑1 (MQ=255)

                                                                                                                         cAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACtt                <  2:738165/141‑1 (MQ=255)

                                                                                                                          aggCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg               <  2:560384/139‑1 (MQ=255)

                                                                                                                          aaGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCCTTCTAGGATGGTCATTACGTg               >  1:11459/1‑141 (MQ=255)

                                                                                                                           aGCATCTGCTAAAGAAGCTTACGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt              <  2:192084/141‑1 (MQ=255)

                                                                                                                             cATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTAt            <  2:347106/141‑1 (MQ=255)

                                                                                                                                cTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCtt         >  1:708848/1‑141 (MQ=255)

                                                                                                                                 tGCTTAAGAAGCGTATGGCTGGATTGTCAGGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATAGGAAGTGTTAAGTGCACGCATTCTAGGATGGCCATTACTTGTATCTTg        <  1:417286/141‑1 (MQ=255)

                                                                                                                                 tGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTg        <  1:832507/141‑1 (MQ=255)

                                                                                                                                   cTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGa      <  1:537006/141‑1 (MQ=255)

                                                                                                                                   cTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGa      <  1:537539/141‑1 (MQ=255)

                                                                                                                                       aGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCa  <  2:310484/141‑1 (MQ=255)

                                                                                                                                       aGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCa  <  2:807009/141‑1 (MQ=255)

GTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCG‑ATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA  >  CP000730/478365‑478639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A