Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A23 F20 I1 R1 21 53.9 1121892 93.4% 1047847 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 478,502 C→A D342E (GAC→GAA)  ndhF → NADH dehydrogenase (ubiquinone)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730478,5020CA98.5% 210.6 / NA 68D342E (GAC→GAA) ndhFNADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (36/31);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTA  >  CP000730/478356‑478646
                                                                                                                                                  |                                                                                                                                                
tttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGc                                                                                                                                                >  1:423535/1‑149 (MQ=255)
 ttATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCCATATTCCACAACAATCATCTGCCAACGAAGCt                                                                                                                                               >  1:14470/1‑149 (MQ=255)
      ttAGTTCAATGTGCATTGGGTGTATATTGAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAACCAAGCATCTGCTAAAGAAGCTTATgg                                                                                                                                          >  1:684105/1‑149 (MQ=255)
      ttAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATgg                                                                                                                                          >  1:128136/1‑149 (MQ=255)
       tAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGc                                                                                                                                         <  1:1034757/149‑1 (MQ=255)
           tCAATGTGCATTGGGTGTATATTCCGCAGAGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGa                                                                                                                                     >  1:578175/1‑149 (MQ=255)
             aaTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                                   >  1:918434/1‑149 (MQ=255)
             aaTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGGGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATggagggagt                                                                                                                                   >  1:34455/1‑142 (MQ=255)
             aaTGCGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATCTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGTTAAAGAAGCTTATGGCTGGAtt                                                                                                                                   >  1:121011/1‑149 (MQ=255)
              aTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTg                                                                                                                                  >  1:74549/1‑149 (MQ=255)
               tgtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGt                                                                                                                                 <  1:767526/149‑1 (MQ=255)
                   cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAGGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATg                                                                                                                             >  1:648239/1‑149 (MQ=255)
                          tgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAGCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACgtg                                                                                                                      >  1:637634/1‑149 (MQ=255)
                             atatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAt                                                                                                                   <  1:254148/149‑1 (MQ=255)
                                ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGTGACGTGTATTAg                                                                                                                >  1:220719/1‑149 (MQ=255)
                                   agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                             >  1:63207/1‑149 (MQ=255)
                                   agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                             >  1:1106080/1‑149 (MQ=255)
                                   agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                             <  1:458527/149‑1 (MQ=255)
                                     cagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtatt                                                                                                           <  1:332392/149‑1 (MQ=255)
                                         gagtaggcATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCg                                                                                                       <  1:252765/142‑1 (MQ=255)
                                         gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGTACGTGTATTAGCTATTATCg                                                                                                       >  1:451985/1‑149 (MQ=255)
                                         gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCg                                                                                                       <  1:834551/149‑1 (MQ=255)
                                          attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATCTTGCAAAACAAGCATCTGCTAAAGAAGCGTATGGCTGGGTTGTCATGGGACGTGTATGAGCCATTATCGt                                                                                                      >  1:50698/1‑149 (MQ=255)
                                              ttCATTTAATATTGCACGGTATTTTTAAAGCAACATGAGGTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCa                                                                                                  >  1:547245/1‑149 (MQ=255)
                                               tCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGCCGTGGATTCGCCCTTATCGTGCCAt                                                                                                 >  1:992812/1‑149 (MQ=255)
                                               tCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAt                                                                                                 >  1:953686/1‑149 (MQ=255)
                                                cATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGCTTCAATATTCCAAATCAAGCATCTGCTAAAGAAGCTTATTGCTGGATTGTCATGGGACGGGTATTAGCTATTATCGTGGCAtt                                                                                                >  1:541630/1‑149 (MQ=255)
                                                  tttAATATTGCACGGTATTTTTAAAGTAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGTCGTGTATTAGCTATTATCGTGGCATTTc                                                                                              <  1:680040/149‑1 (MQ=255)
                                                   ttAATATTGCACGGTATTTTTAGAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                             >  1:733617/1‑149 (MQ=255)
                                                    tAATACTCCACGGTATTTTTAAACCAACATTATTCTTCCAACCAGTTTCTATCGTGAAGCGAGTCACTATACCAAAACAAGCCTCTGCTAAAGAACCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTa                                                                                            <  1:56969/149‑1 (MQ=255)
                                                       tatTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                         <  1:742408/149‑1 (MQ=255)
                                                        atTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtttt                                                                                        <  1:410895/149‑1 (MQ=255)
                                                         ttGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGATTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTg                                                                                       >  1:215660/1‑149 (MQ=255)
                                                             aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCTTGGGACGTGTTTTAGCTATTATCGTGGTATTTCTATTTTGGGTg                                                                                   >  1:55116/1‑149 (MQ=255)
                                                             aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                                   >  1:642879/1‑149 (MQ=255)
                                                             aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                                   <  1:468589/149‑1 (MQ=255)
                                                             aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                                   >  1:162485/1‑149 (MQ=255)
                                                                 tATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagta                                                                               <  1:335393/149‑1 (MQ=255)
                                                                 tATTTTCAAAGCAACATTAGTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGGTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagta                                                                               <  1:495372/149‑1 (MQ=255)
                                                                    ttttAAAGCAACATTATTTTTCAAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTg                                                                            <  1:103335/149‑1 (MQ=255)
                                                                    ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTg                                                                            <  1:369865/149‑1 (MQ=255)
                                                                     tttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGCCCTGGGTCGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGGAGTgc                                                                           >  1:313678/1‑148 (MQ=255)
                                                                       tAAAGCAACATTATTTTTACAATAAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACa                                                                         >  1:832837/1‑149 (MQ=255)
                                                                        aaaGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGTTGGAGTGTCATGGTACGTGTCTTAGCTATTATCGTGGAATTTCTATTTTGAATGAGAACTGACAg                                                                        >  1:538467/1‑149 (MQ=255)
                                                                           gCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCCTTTCTATTTTGGATGAGTACTGACAGAAg                                                                     >  1:931233/1‑149 (MQ=255)
                                                                           gCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAg                                                                     <  1:987901/149‑1 (MQ=255)
                                                                              aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGCTTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGGGACAGAAGTGc                                                                  >  1:67904/1‑149 (MQ=255)
                                                                              aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                                  >  1:381194/1‑149 (MQ=255)
                                                                              aCATTATTTTTACAACCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                                  <  1:14700/149‑1 (MQ=255)
                                                                              aCATGATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                                  <  1:896660/149‑1 (MQ=255)
                                                                               cATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCa                                                                 <  1:415437/149‑1 (MQ=255)
                                                                                      tttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                          >  1:549931/1‑149 (MQ=255)
                                                                                      tttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGAGTTTGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGGATTTCTATTTTGGATGAGTAGTGACAGAGGTGCATGTGAAg                                                          >  1:652121/1‑149 (MQ=255)
                                                                                       ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATTAAgt                                                         >  1:1031522/1‑149 (MQ=255)
                                                                                       ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgt                                                         <  1:793997/149‑1 (MQ=255)
                                                                                              cAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                                  >  1:582277/1‑149 (MQ=255)
                                                                                                         ctGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCt                                       <  1:879818/148‑1 (MQ=255)
                                                                                                                      atatTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                          <  1:945251/149‑1 (MQ=255)
                                                                                                                         ttCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACtt                       <  1:799797/149‑1 (MQ=255)
                                                                                                                          tCCAACACAAGCATCTGCTAAAGAAGCTTATGGCTGGAGTGTCATGGGACGTTTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg                      <  1:1048553/149‑1 (MQ=255)
                                                                                                                          tCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg                      <  1:707924/149‑1 (MQ=255)
                                                                                                                           ccAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt                     >  1:597453/1‑149 (MQ=255)
                                                                                                                                 cAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTg               <  1:848307/149‑1 (MQ=255)
                                                                                                                                  acgCACCTTCTAAAGAAGCTCACGGCGTGATTGTCATGGGCCGTCTATTAGCTATTATCCTGGCATTTCTATTTTGGATGAGTAGTGACATAAGTGCATATGAAGTGTTAAGTGCACTCACTCTAGGATGGTCATTACTTGTATCTTgg              <  1:98758/147‑1 (MQ=255)
                                                                                                                                  aaGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTgg              <  1:380338/149‑1 (MQ=255)
                                                                                                                                  aaGCATCGGCTAAAGAAGGTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACGCATTCTAGTATGGTCATTACTTGTATCTTgg              <  1:734348/149‑1 (MQ=255)
                                                                                                                                     cATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTAGTATCGTGGCATTTCTATTGTGGGTGCGCCGTCACATAGGGGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAAt           <  1:265565/149‑1 (MQ=255)
                                                                                                                                         tGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGGATCaaa       >  1:573170/1‑149 (MQ=255)
                                                                                                                                              aagaagCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTa  >  1:251086/1‑149 (MQ=255)
                                                                                                                                              aagaagCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTa  >  1:1032463/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTTATGTTAGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAATGGTA  >  CP000730/478356‑478646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A