Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F106 I0 R1
|
88 |
30.3 |
670850 |
93.2% |
625232 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
478,502 |
C→A |
100% |
D342E (GAC→GAA) |
ndhF → |
NADH dehydrogenase (ubiquinone) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 478,502 | 0 | C | A | 100.0%
| 83.2
/ NA
| 29 | D342E (GAC→GAA) | ndhF | NADH dehydrogenase (ubiquinone) |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/15); total (14/15) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATC > CP000730/478366‑478638
|
ttCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCtt > 1:295063/1‑141 (MQ=255)
cAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt > 1:38355/1‑141 (MQ=255)
tgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCCTCTGCTAAAGAAGCTTATGGCTg > 1:292686/1‑141 (MQ=255)
cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGa > 1:123851/1‑141 (MQ=255)
gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa < 1:207359/141‑1 (MQ=255)
gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa < 1:29721/141‑1 (MQ=255)
tttttCAGCATCTGTTATTTATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATCTTCTAAGACAAGCATCTGCTAATGTAGCTTATGGCTGGATTGTCATggg < 2:152556/138‑1 (MQ=255)
atatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATggg < 1:188157/141‑1 (MQ=255)
ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCTTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg < 1:132879/141‑1 (MQ=255)
agcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAtt > 2:101435/1‑141 (MQ=255)
gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCCTGGGACGTGTATTAGc > 2:13148/1‑141 (MQ=255)
attattAATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt < 2:144168/141‑1 (MQ=255)
cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTATATCGTGAAGCGATTTCATTTTTGAGAACAAGCAGCGGTGTAAGAATCTTATGGCGGGGTTGTCATAGGACGTGTATTAGCTATTATCCTGGCATTTCTAtt > 1:147636/1‑141 (MQ=255)
ggTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGGGAAGCGATTCAATATTCCCAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt < 1:39598/141‑1 (MQ=255)
ggTATTTTAGAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt < 1:309763/141‑1 (MQ=255)
aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg < 2:268638/141‑1 (MQ=255)
tttAAAGCAACATTATTTTCACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCTTGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg < 2:219158/141‑1 (MQ=255)
tAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTCTTATCGTGGCATTTCTATTTTGGATggg > 2:243360/1‑139 (MQ=255)
ttattTTTACAATCAGGGTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGa < 2:123851/141‑1 (MQ=255)
tCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATTGATGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTCTTTTGGGTGAGTAGTGTCAGGAGGGCATATGag > 2:106398/1‑140 (MQ=255)
ggTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtg > 1:124527/1‑141 (MQ=255)
cTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTaa > 1:86078/1‑141 (MQ=255)
tCGGGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTg < 1:155440/141‑1 (MQ=255)
tgttaaGTGATTCAATATGCCAAACAAAGCATCTGTTCAAGAAGTTTATGTCTTGATTGTCTTGGGACGTGTATTAGCTATGATCGTGGCATTTCTATTTTGGATGAGTAGTTACAGAAGTGCATATGAAGTTTTAAGTGc < 2:303663/137‑1 (MQ=255)
gAATCGATTTAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt < 2:128868/141‑1 (MQ=255)
aGCATCTTCTAAAGAAGCTTATGGCTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt < 2:295063/141‑1 (MQ=255)
aGCATCTGCTAAAGAAGCTTATGGCTGGCTTGTCCTGGGACGTGAATTAGGTATTCCGCTGGCATTTCTATTTTGGATGAGAAGTGACAGAAGTGCATCTGAAGTGTTAAGTGCACTACTTCTAGGATGTTAATGACTTGt > 1:63385/1‑141 (MQ=255)
gCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTa > 2:73939/1‑141 (MQ=255)
aaaGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAAt > 2:31207/1‑141 (MQ=255)
aagaagCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAATTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATGCTAGGATGGTCATACCTAGTAACTTGGaaac > 2:227348/1‑139 (MQ=255)
|
TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATC > CP000730/478366‑478638
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
