Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F70 I1 R1 11 51.9 1081775 93.5% 1011459 258.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,685,527 A→G G354G (GGT→GGC)  fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5270AG97.2% 122.6 / NA 37G354G (GGT→GGC) fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (21/14);  minor base T (0/1);  total (22/15)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAA  >  NC_000913/1685268‑1685795
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                            
cAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTCATTGCTTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATGGCAGGAAATTGTGTATCACCATTTGCAGAAAGACGTTCGGGTCAAAGTTCCCGGAATCGcccccc                                                                                                                                                                                                                                                               >  4:52487/1‑275 (MQ=255)
   gggCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGAACTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCCGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAATCGCACCGATTGCAGGAAATTGTTGTTCACCATTGGACGGAAGACCTTCAGTTCAAAGTTGCCCGAAACGCACCCCAt                                                                                                                                                                                                                                                             >  3:63330/1‑274 (MQ=255)
        gCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAACCTTTCCATGCCATCTGCCAGCAAGCGCAACGATTGCAGGAAATTGTGGATCACCATTGGAGGGAAGACTTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCagtctga                                                                                                                                                                                                                                                        >  4:91926/1‑268 (MQ=255)
        gCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCAGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGAt                                                                                                                                                                                                                                                       <  1:161354/275‑1 (MQ=255)
                    gTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCTGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcgt                                                                                                                                                                                                                                            <  3:165210/274‑1 (MQ=255)
                       aGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTCAAGGAAATTGTGGATCACCATTGGACGGAAGCCGGTCAGTTCAAAGTTGCCTGAAGTCCCCCCCATGTTTAACGCCAccgctgtccc                                                                                                                                                                                                                                        >  3:131107/1‑266 (MQ=255)
                                                           gcTTTGTCATAACCAATGTGGGTGTTAAGCGCACTCACCAGCATCAGCTATTCATTGAGTAATTGATTGATTCGCTCACGCTTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                                                                                                                                                    <  4:27281/275‑1 (MQ=255)
                                                                 tCATAACCAATGTTGGTGTTAAGCGCATTCACCAGCATCAGTGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                                                                                                                                                              <  1:36116/275‑1 (MQ=255)
                                                                      aCCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCACATGTTGATCGCCACGTAGTTCCCCATCCCCTGACAGACGAGCAGGGTTAATGCCTcaccccatcttggc                                                                                                                                                                                          >  4:72474/1‑263 (MQ=255)
                                                                           tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTNTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCCCCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCACCACCGGTCCCCAGAACCTCGGTTATGCCTCACACGTTCTTGGGTTCCCt                                                                                                                                                                                    >  4:141447/1‑275 (MQ=255)
                                                                           tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACt                                                                                                                                                                                    >  1:59046/1‑275 (MQ=255)
                                                                           tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGAAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCCAGTCGTTCCCCATCACCTGACAGCAAAACATGGTTAATGCCTAACACTGTGTGGGGTTCACt                                                                                                                                                                                    >  4:247701/1‑275 (MQ=255)
                                                                                             tCACCAGCATCAGCGATTCATTGAGTAATTGATTGATACGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcc                                                                                                                                                                                                                                                                   >  4:230058/1‑178 (MQ=255)
                                                                                                 cAGCATCAGCGATTCATTGAGTAATTGATTGATACGATCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccccc                                                                                                                                                                                                                                                               <  2:230058/178‑1 (MQ=255)
                                                                                                                              tgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCAACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGTCTCATTTTCCGGGATTGAGAttgc                                                                                                                                  >  1:212159/1‑272 (MQ=255)
                                                                                                                                       cACGCTTCGGTTGAAGACCCACTGGGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCg                                                                                                                        <  3:212158/275‑1 (MQ=255)
                                                                                                                                            ttCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAgcg                                                                                                                   <  3:59046/275‑1 (MQ=255)
                                                                                                                                               ggTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCAATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCGGAGCATGGTTAATGCCTCACACTGTGTTCGGTTCACTTTCCCCGGCATGATTCAGCTTGCTGGCTCATTGTCCGGGATTGAGATTTCTCTATTTCCGCCGTGCgg                                                                                                                >  3:48146/1‑275 (MQ=255)
                                                                                                                                                  tCAATACCCACTGCGCAGTGTTTGTTAAGCCTTTCCATGCCATCTGCCAGCAAGCGGACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGcc                                                                                                             <  1:63330/275‑1 (MQ=255)
                                                                                                                                                         ccccTGCGCAGTGTTGGTTAAAACTTTCCATGCCATATGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGTAAGACGTTCAGTTCAAAGTTTCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgcc                                                                                                      <  1:81923/272‑1 (MQ=255)
                                                                                                                                                               ggcATTGTTGGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTATGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTc                                                                                                                                                 <  4:110372/225‑1 (MQ=255)
                                                                                                                                                                 aagggtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGGTTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCg                                                                                              <  2:72474/271‑1 (MQ=255)
                                                                                                                                                                    tgtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCCTGATTGAGCTGCCCGGCTCGTTTTCCGGGATTGAGATTTCCCCAATTCCGCAGCCCGGGCCAGAGgcccgcacgctg                                                                                             >  3:144951/1‑267 (MQ=255)
                                                                                                                                                                    tgtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCACATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCggcc                                                                                           >  3:83793/1‑273 (MQ=255)
                                                                                                                                                                         gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCCCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGAcatca                                                                                       >  3:160480/1‑274 (MQ=255)
                                                                                                                                                                                cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGCTCAGTTCAAAGTTGCTGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAGGGTCAATGCCTCACCCTGTGTTTGGTTCACTTTCCCCGGCATGATTGCGCTGCCCGGCTCACTTTCCGGGATTGAGATTGCACCAATTCTGCAGAGCGGGCCAGAGGCCACCCAGTGGCcatactagccgc                                                                                >  1:79779/1‑265 (MQ=255)
                                                                                                                                                                                   tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                                                                                                                                                                                        >  1:100117/1‑151 (MQ=255)
                                                                                                                                                                                       tGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                                                                                                                                                    <  3:100117/151‑1 (MQ=255)
                                                                                                                                                                                         ccgtCTGCCAGCAAGCGGACCGCTTTCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTGCAAAGTTGCCGGAAGCGCCCCCCATTTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAg                                                                      <  2:91926/272‑1 (MQ=255)
                                                                                                                                                                                             cTGCCAGCAAGCGCACCGAGTGCAGGAAAGTGTGGATCACCATTGGACGGAATACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGGTCGCCACGTCGTTCCCCTTCACCTGACAGCAGAGCATGGTTAATGCCTCCCACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCCGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGAc                                                                  <  2:52487/275‑1 (MQ=255)
                                                                                                                                                                                                                       aaaTTGTGGGTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCg                                          <  2:250347/273‑1 (MQ=255)
                                                                                                                                                                                                                             tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCCCAGCGCGGGCCAGAGGCCAGCCAGCGGACCTCATTCGCGATTTTCATCAGTTACCGAGCCCACCCTCTCAAccgccctttccccc                                   >  4:226524/1‑261 (MQ=255)
                                                                                                                                                                                                                                            cGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGCCATCATTGGCGATTTTCATCAGTGCCGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGACACAGGACATcac                    >  1:157466/1‑274 (MQ=255)
                                                                                                                                                                                                                                               aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCCGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCATCCAACCCTTTCAACGCGCCGGTCCGCTGAACCAAGGCATCACAGGt                >  2:263/1‑275 (MQ=255)
                                                                                                                                                                                                                                               aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCCTTGGCGATTATCATCAGTGTCGCAGCCAACCCATTCAACGCCCCTTCCGCCTGAACCCGGGCATCACAGGt                >  4:253203/1‑275 (MQ=255)
                                                                                                                                                                                                                                                         gttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCCCAGGTCGCCAccgc       >  1:180387/1‑274 (MQ=255)
                                                                                                                                                                                                                                                             aaaGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGAACTCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACTCGCCGTGCGCCTGAACCAGGGCATCACACGTCGCCAgccgttcca  >  1:162273/1‑268 (MQ=255)
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                            
CAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAA  >  NC_000913/1685268‑1685795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A