Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F195 I0 R2	483	52.1	1908604	98.1%	1872340	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,685,527	A→G	100%	G354G (GGT→GGC)	fumC ←	fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,685,527	0	A	G	100.0%	149.4 / NA	42	G354G (GGT→GGC)	fumC	fumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/25); total (17/25)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTT  >  NC_000913/1685389‑1685666

ttgattgaTTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcgc                                                                                                                                   >  3:89407/1‑149 (MQ=255)

   attgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCTCCGATTGCAGGAAAGTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccc                                                                                                                                <  4:35104/149‑1 (MQ=255)

   attgattAACTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAGAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATAGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccc                                                                                                                                >  2:370966/1‑149 (MQ=255)

    ttgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGccccc                                                                                                                               <  1:226204/149‑1 (MQ=255)

             tCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGAt                                                                                                                      <  2:315573/149‑1 (MQ=255)

              cACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATc                                                                                                                     <  3:276645/149‑1 (MQ=255)

                        ttCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcgt                                                                                                           >  3:112589/1‑149 (MQ=255)

                        ttCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcgt                                                                                                           >  4:59086/1‑149 (MQ=255)

                            aTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                       <  4:115391/149‑1 (MQ=255)

                            aTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                       >  1:122632/1‑149 (MQ=255)

                             tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGAGCGCCACGTCGTTcccc                                                                                                      <  1:343841/149‑1 (MQ=255)

                                      cgcAGTGTTGGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGACACGTCGTTCCCCATCACCTGa                                                                                             >  1:409217/1‑149 (MQ=255)

                                        cAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAca                                                                                           <  3:59086/149‑1 (MQ=255)

                                            gtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                       >  2:321544/1‑149 (MQ=255)

                                             tttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAt                                                                                                                            <  1:144935/111‑1 (MQ=255)

                                             tttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAt                                                                                                                            >  2:144935/1‑111 (MQ=255)

                                             tttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                                                                      <  2:455673/149‑1 (MQ=255)

                                               tgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                    <  2:131667/149‑1 (MQ=255)

                                                   aaaaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATgg                                                                                <  4:196714/149‑1 (MQ=255)

                                                   aaaaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATgg                                                                                <  4:112589/149‑1 (MQ=255)

                                                    aaaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                    >  3:18735/1‑144 (MQ=255)

                                                    aaaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                    <  4:18735/144‑1 (MQ=255)

                                                       cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTaa                                                                            <  3:270560/149‑1 (MQ=255)

                                                       cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTaa                                                                            <  2:332016/149‑1 (MQ=255)

                                                        tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                           >  3:337412/1‑149 (MQ=255)

                                                          tCCATGCCATCTGCCAGCAAGTGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGc                                                                         <  3:372437/149‑1 (MQ=255)

                                                               gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTc                                                                      >  1:160165/1‑147 (MQ=255)

                                                               gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTc                                                                      <  2:160165/147‑1 (MQ=255)

                                                                 cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                                                             >  2:435363/1‑122 (MQ=255)

                                                                 cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                                                             <  1:435363/122‑1 (MQ=255)

                                                                         aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                          <  3:406362/149‑1 (MQ=255)

                                                                          gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                         <  2:122632/149‑1 (MQ=255)

                                                                               cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCAc                                                    <  2:460722/149‑1 (MQ=255)

                                                                                              aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGAt                                     <  4:337412/149‑1 (MQ=255)

                                                                                                        tCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGccc                           <  2:409217/149‑1 (MQ=255)

                                                                                                            cATTTGACTGAAGACGTTCAGTTCAAAGTTGCCGGCAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCTATCACCTTACAGCAGAGCATGGTTAATGCCTCACACTGTGTCGGGTTCACTTTCCCCGGCACGATTGAGCTGCCCGGCt                       <  1:370966/149‑1 (MQ=255)

                                                                                                                     gAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                              <  4:317252/117‑1 (MQ=255)

                                                                                                                     gAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCg              >  3:379213/1‑149 (MQ=255)

                                                                                                                     gAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTGTccc                                              >  3:317252/1‑117 (MQ=255)

                                                                                                                      aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCgg             >  3:385254/1‑149 (MQ=255)

                                                                                                                            ttcagttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTga       >  3:247743/1‑149 (MQ=255)

                                                                                                                                 ttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAttt  >  2:442602/1‑149 (MQ=255)

TTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTT  >  NC_000913/1685389‑1685666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A