Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,036,505 |
G→A |
100% |
A632A (GCG→GCA) |
rsxC → |
fused predicted 4Fe‑4S ferredoxin‑type protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,036,505 | 0 | G | A | 100.0%
| 111.1
/ NA
| 40 | A632A (GCG→GCA) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/16); total (24/16) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC > minE/1036437‑1036571
|
gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:798428/1‑71 (MQ=255)
cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:772823/1‑70 (MQ=25)
ggAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAgcacgc > 1:459625/1‑70 (MQ=255)
aaCCAGAAGAACAGGTCGATCCGCGCAAAGCC‑CCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc < 1:1833412/71‑1 (MQ=11)
cAGAAGAACAGGTCGATCCGCTCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt < 1:3212726/70‑1 (MQ=255)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:2485518/1‑70 (MQ=25)
gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcgc > 1:3373221/1‑63 (MQ=35)
gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt > 1:183416/1‑68 (MQ=25)
gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa > 1:3009037/1‑69 (MQ=14)
gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACa < 1:2058078/71‑1 (MQ=14)
aGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc < 1:249744/71‑1 (MQ=255)
aGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc < 1:2187849/71‑1 (MQ=255)
gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc > 1:1555767/1‑70 (MQ=255)
ccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 1:106718/1‑71 (MQ=255)
cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACATGCTAATGCCGAg > 1:1682496/1‑64 (MQ=11)
cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:18572/1‑64 (MQ=255)
cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATCCCGAGcc > 1:2280237/1‑63 (MQ=14)
gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac < 1:3566345/70‑1 (MQ=255)
gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa > 1:3472898/1‑69 (MQ=255)
gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3241093/1‑65 (MQ=255)
aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg < 1:464473/71‑1 (MQ=255)
aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg < 1:2773291/71‑1 (MQ=255)
gCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt > 1:46455/1‑71 (MQ=255)
gCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt > 1:1703271/1‑71 (MQ=255)
cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:2433664/71‑1 (MQ=255)
gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa > 1:272085/1‑71 (MQ=255)
cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt > 1:123193/1‑71 (MQ=255)
aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:42014/1‑71 (MQ=255)
aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:2207298/1‑71 (MQ=255)
aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:2109224/1‑71 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCg > 1:269563/1‑46 (MQ=255)
cccGTGCCAAAGCACGCAAGCTGTAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATc > 1:471707/1‑66 (MQ=255)
ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:107877/1‑69 (MQ=255)
ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa < 1:2209505/71‑1 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACTACAGGTCGATCCGCGCaa < 1:1925978/71‑1 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa < 1:3491597/71‑1 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa < 1:3318133/71‑1 (MQ=255)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa > 1:2315616/1‑69 (MQ=255)
tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg > 1:3480849/1‑71 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcc < 1:1755889/71‑1 (MQ=255)
aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc > 1:3073811/1‑71 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc < 1:14420/70‑1 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc < 1:1370703/70‑1 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc > 1:1261168/1‑69 (MQ=255)
|
GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC > minE/1036437‑1036571
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
