Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R1
|
67 |
74.8 |
3863507 |
93.7% |
3620106 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,036,505 |
G→A |
100% |
A632A (GCG→GCA) |
rsxC → |
fused predicted 4Fe‑4S ferredoxin‑type protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,036,505 | 0 | G | A | 100.0%
| 106.7
/ NA
| 42 | A632A (GCG→GCA) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/20); total (22/20) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC > minE/1036440‑1036571
|
gAACCAGAAGAACATGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa < 1:1816116/71‑1 (MQ=255)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg < 1:2496121/71‑1 (MQ=21)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg < 1:3164190/71‑1 (MQ=21)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg < 1:2101116/71‑1 (MQ=21)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg < 1:3587209/71‑1 (MQ=21)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg < 1:1321353/71‑1 (MQ=21)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg < 1:1279834/71‑1 (MQ=21)
agaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa < 1:1776993/70‑1 (MQ=18)
agaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa < 1:3289955/70‑1 (MQ=18)
gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa > 1:2226277/1‑69 (MQ=14)
gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACa < 1:327563/71‑1 (MQ=14)
cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCg < 1:2919503/71‑1 (MQ=17)
cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCg < 1:348473/71‑1 (MQ=17)
aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:271471/1‑71 (MQ=25)
gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:1422065/1‑70 (MQ=255)
gTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 1:3551944/1‑69 (MQ=255)
gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac < 1:3390124/70‑1 (MQ=255)
aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg > 1:2088715/1‑71 (MQ=255)
aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg > 1:1903632/1‑71 (MQ=255)
aaGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGACGAGCCAGAACAACAg > 1:850748/1‑71 (MQ=14)
ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc < 1:1170463/71‑1 (MQ=255)
gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa > 1:1730761/1‑71 (MQ=255)
aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:1621358/1‑71 (MQ=255)
aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:3521458/1‑71 (MQ=255)
tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:2380671/1‑71 (MQ=255)
ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa < 1:2989218/71‑1 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa < 1:3316128/71‑1 (MQ=255)
gTGCCAAAGCACGCACGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa < 1:1639444/49‑1 (MQ=14)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 1:2741623/1‑50 (MQ=255)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 1:1237919/1‑50 (MQ=255)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 1:2168550/1‑50 (MQ=255)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa > 1:1621396/1‑69 (MQ=255)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa > 1:2479609/1‑69 (MQ=255)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCaaa < 1:1700289/71‑1 (MQ=255)
gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa < 1:2479199/69‑1 (MQ=255)
gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgc > 1:3787285/1‑71 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:2368610/1‑48 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:1497686/1‑48 (MQ=255)
aaGCACGCAAGCTGGAACTGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc > 1:420351/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc > 1:2174292/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc > 1:2079894/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc < 1:3526133/71‑1 (MQ=255)
|
GAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC > minE/1036440‑1036571
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
