Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,036,505 |
G→A |
100% |
A632A (GCG→GCA) |
rsxC → |
fused predicted 4Fe‑4S ferredoxin‑type protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,036,505 | 0 | G | A | 100.0%
| 114.5
/ NA
| 41 | A632A (GCG→GCA) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/19); total (22/19) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG > minE/1036440‑1036572
|
gAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa < 1:2103861/71‑1 (MQ=21)
aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg > 1:5337762/1‑71 (MQ=21)
ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc > 1:4111870/1‑70 (MQ=25)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:5184774/1‑70 (MQ=25)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:3296170/1‑70 (MQ=25)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:3084337/1‑70 (MQ=25)
agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:2263812/1‑70 (MQ=25)
gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa > 1:3650996/1‑69 (MQ=14)
gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc < 1:984798/71‑1 (MQ=14)
cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCg < 1:5014398/71‑1 (MQ=17)
gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc > 1:823914/1‑70 (MQ=255)
gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc > 1:513075/1‑70 (MQ=255)
gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc > 1:1392301/1‑70 (MQ=255)
cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg < 1:664374/71‑1 (MQ=255)
cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg < 1:5166738/71‑1 (MQ=255)
gccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa < 1:3198784/71‑1 (MQ=255)
ccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg < 1:368064/69‑1 (MQ=255)
tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa > 1:2552361/1‑71 (MQ=255)
tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa < 1:1750338/71‑1 (MQ=255)
gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa > 1:3164791/1‑69 (MQ=255)
cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:2476653/62‑1 (MQ=255)
cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:1703906/62‑1 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt > 1:5453208/1‑66 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt > 1:3916682/1‑66 (MQ=255)
cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:4192298/1‑70 (MQ=255)
tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg < 1:1994576/71‑1 (MQ=255)
gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgc > 1:1098002/1‑71 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:5084762/48‑1 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcc < 1:3858958/71‑1 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcc > 1:5071770/1‑71 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgc < 1:1598222/70‑1 (MQ=255)
cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg < 1:1201008/44‑1 (MQ=255)
cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg < 1:3154869/44‑1 (MQ=255)
cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg < 1:2494239/44‑1 (MQ=255)
aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa < 1:869899/48‑1 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATc > 1:5601201/1‑57 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:5032083/1‑37 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa < 1:3424281/46‑1 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 1:4799958/1‑43 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa > 1:1238943/1‑43 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGccgccg > 1:2665409/1‑71 (MQ=255)
|
GAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG > minE/1036440‑1036572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
