Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F3 I0 R2 88 130.8 6963722 93.6% 6518043 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,036,505 G→A 100% A632A (GCG→GCA)  rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,036,5050GA100.0% 216.2 / NA 77A632A (GCG→GCA) rsxCfused predicted 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/34);  total (43/34)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC  >  minE/1036437‑1036571
                                                                    |                                                                  
gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:2994742/1‑71 (MQ=21)
gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:4212221/1‑71 (MQ=21)
gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:124884/1‑71 (MQ=21)
 cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:3979876/1‑70 (MQ=25)
 cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:531754/1‑70 (MQ=25)
 cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:4837214/1‑70 (MQ=25)
 cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:553705/1‑70 (MQ=25)
 cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:1808136/1‑70 (MQ=25)
 cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:4698823/1‑70 (MQ=25)
      ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg                                                              <  1:4281267/69‑1 (MQ=25)
      ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg                                                              <  1:6662967/69‑1 (MQ=25)
      ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg                                                              <  1:1040096/69‑1 (MQ=25)
      ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                            <  1:1455337/71‑1 (MQ=21)
      ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                            <  1:3214807/71‑1 (MQ=21)
      ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                            <  1:630326/71‑1 (MQ=21)
      ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                            >  1:1160173/1‑71 (MQ=21)
      ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                            <  1:2962894/71‑1 (MQ=21)
        agaagaACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg                                                          <  1:4883906/71‑1 (MQ=21)
        agaagaACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg                                                          <  1:5641586/71‑1 (MQ=255)
         gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTgg                                                          >  1:2282763/1‑70 (MQ=25)
            gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                        >  1:5690347/1‑69 (MQ=14)
            gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                        >  1:4927862/1‑69 (MQ=14)
            gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                        >  1:2892476/1‑69 (MQ=14)
                               aaGCCGCCGTCGAAGCCGCTATTGCCCGTTCCAAAGCACGCAAGCTCGAACAGCAACAGGCTAATGCCGa                                    >  1:2447910/1‑70 (MQ=11)
                                   cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                  >  1:933525/1‑68 (MQ=255)
                                   cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                  >  1:518535/1‑68 (MQ=255)
                                   cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                  >  1:5759013/1‑68 (MQ=255)
                                   cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                <  1:5050434/70‑1 (MQ=255)
                                   cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg                               <  1:5351282/71‑1 (MQ=255)
                                      cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                            <  1:5496349/71‑1 (MQ=255)
                                        tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:5225699/71‑1 (MQ=255)
                                        tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:6360408/71‑1 (MQ=255)
                                        tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                            <  1:5716915/69‑1 (MQ=255)
                                        tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                            <  1:6461294/69‑1 (MQ=255)
                                          gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:4419111/1‑69 (MQ=255)
                                          gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:1009372/1‑69 (MQ=255)
                                          gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        <  1:1532488/71‑1 (MQ=255)
                                          gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        <  1:4943916/71‑1 (MQ=255)
                                             gCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt                     <  1:609802/71‑1 (MQ=255)
                                                 cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                 >  1:6333813/1‑71 (MQ=255)
                                                     tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                <  1:5498605/52‑1 (MQ=255)
                                                     tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc             >  1:578592/1‑71 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:1133156/1‑57 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:714708/1‑57 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                 >  1:572746/1‑66 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg            >  1:1588381/1‑71 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg            >  1:5826619/1‑71 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg            >  1:4433412/1‑71 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg            >  1:5110008/1‑71 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg            >  1:64976/1‑71 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc             >  1:302289/1‑70 (MQ=255)
                                                       cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              >  1:3108912/1‑52 (MQ=255)
                                                       cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              >  1:1001552/1‑52 (MQ=255)
                                                       cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              >  1:5952924/1‑52 (MQ=255)
                                                       cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc           <  1:80389/71‑1 (MQ=255)
                                                       cccGTGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              >  1:3351617/1‑52 (MQ=14)
                                                          gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc           >  1:5401841/1‑68 (MQ=255)
                                                          gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa        <  1:354520/71‑1 (MQ=255)
                                                          gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa          >  1:5727553/1‑69 (MQ=255)
                                                          gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa          >  1:4348684/1‑69 (MQ=255)
                                                          gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa          >  1:5550964/1‑69 (MQ=255)
                                                          gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCa          >  1:6636386/1‑69 (MQ=255)
                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac                         <  1:2783405/53‑1 (MQ=255)
                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:6452255/52‑1 (MQ=255)
                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:2031219/52‑1 (MQ=255)
                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:2860047/52‑1 (MQ=255)
                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:3761478/52‑1 (MQ=255)
                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                            <  1:3013454/50‑1 (MQ=255)
                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg       <  1:1417922/71‑1 (MQ=255)
                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg       <  1:3801732/71‑1 (MQ=255)
                                                            gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa        <  1:5035128/69‑1 (MQ=255)
                                                            gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa        <  1:3544121/69‑1 (MQ=255)
                                                            gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgc      >  1:6083341/1‑71 (MQ=255)
                                                            gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgc      >  1:3205018/1‑71 (MQ=255)
                                                             ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcc     <  1:4544848/71‑1 (MQ=255)
                                                              cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccg    >  1:1877048/1‑71 (MQ=255)
                                                              cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccg    >  1:2190983/1‑71 (MQ=255)
                                                              cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccg    >  1:3203455/1‑71 (MQ=255)
                                                               aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        >  1:5687523/1‑50 (MQ=255)
                                                               aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        >  1:2594836/1‑50 (MQ=255)
                                                               aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        >  1:1417089/1‑50 (MQ=255)
                                                               aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca                        >  1:3629703/1‑50 (MQ=255)
                                                               aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt                     <  1:5350038/53‑1 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:2451452/1‑71 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:2924844/1‑71 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:1130474/1‑71 (MQ=255)
                                                                    |                                                                  
GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC  >  minE/1036437‑1036571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A