Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F3 I0 R1
|
41 |
86.6 |
4450826 |
94.1% |
4188227 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,036,505 |
G→A |
100% |
A632A (GCG→GCA) |
rsxC → |
fused predicted 4Fe‑4S ferredoxin‑type protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,036,505 | 0 | G | A | 98.2%
| 121.3
/ ‑5.5
| 48 | A632A (GCG→GCA) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (29/18); minor base C (0/1); total (29/19) |
| Fisher's exact test for biased strand distribution p-value = 3.96e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG > minE/1036438‑1036572
|
cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:2997194/1‑70 (MQ=25)
cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg < 1:3306334/70‑1 (MQ=25)
ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt > 1:626171/1‑71 (MQ=21)
cAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:1691315/1‑71 (MQ=21)
aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacgc > 1:1957299/1‑62 (MQ=25)
aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa > 1:1563277/1‑64 (MQ=25)
agaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCAGTGCCAAAGCACGCAAGCTg > 1:3191490/1‑67 (MQ=255)
gaaCAGATCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa > 1:2760775/1‑69 (MQ=255)
gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc < 1:1744277/71‑1 (MQ=14)
aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:3762797/1‑71 (MQ=25)
aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:425037/1‑71 (MQ=25)
aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg > 1:1286372/1‑71 (MQ=25)
gccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg > 1:3145908/1‑70 (MQ=255)
ccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc < 1:4229799/62‑1 (MQ=14)
cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:1240979/71‑1 (MQ=255)
gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaac < 1:4159477/70‑1 (MQ=255)
gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca < 1:2915994/71‑1 (MQ=255)
cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:3642724/71‑1 (MQ=255)
gCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:3274149/61‑1 (MQ=255)
gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:664645/70‑1 (MQ=255)
gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:2785947/70‑1 (MQ=255)
gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCg < 1:3968991/70‑1 (MQ=255)
tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt > 1:305658/1‑70 (MQ=255)
tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:1531241/1‑71 (MQ=255)
tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:1788886/1‑71 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2604101/1‑53 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:1667186/1‑71 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:1227596/1‑71 (MQ=255)
cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:3525823/64‑1 (MQ=255)
cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:917563/64‑1 (MQ=255)
cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:2316622/64‑1 (MQ=255)
cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg < 1:2613876/70‑1 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATc > 1:2107620/1‑64 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATc > 1:1248955/1‑64 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa < 1:1738119/71‑1 (MQ=255)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCTCaaa < 1:2964298/71‑1 (MQ=255)
gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa < 1:425204/71‑1 (MQ=255)
tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa < 1:4242266/70‑1 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3118580/1‑46 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2600145/1‑46 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2723222/1‑46 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2304922/1‑46 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3749038/1‑46 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3688866/1‑46 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:3633999/1‑46 (MQ=255)
ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:1823360/1‑46 (MQ=255)
cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaa < 1:1683490/66‑1 (MQ=255)
aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc > 1:1704835/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg > 1:653030/1‑66 (MQ=255)
aaGCACACAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAg > 1:571755/1‑66 (MQ=255)
aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGccgccg > 1:2915859/1‑71 (MQ=255)
|
CGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG > minE/1036438‑1036572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
