Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F8 I0 R1
|
22 |
59.8 |
3060272 |
94.8% |
2901137 |
61.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,036,505 |
G→A |
100% |
A632A (GCG→GCA) |
rsxC → |
fused predicted 4Fe‑4S ferredoxin‑type protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,036,505 | 0 | G | A | 100.0%
| 94.6
/ NA
| 36 | A632A (GCG→GCA) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/9); total (27/9) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC > minE/1036437‑1036571
|
gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:1467111/1‑71 (MQ=21)
gCGGAACCAGAAGAACAGGTCGATCCGCACAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:300478/1‑71 (MQ=255)
gCGGAACCAGAAGAACAGGTCGATCCGCACAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:296855/1‑71 (MQ=255)
cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg > 1:1793323/1‑70 (MQ=25)
ggAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCa < 1:2724390/71‑1 (MQ=21)
gAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacgc > 1:2846014/1‑69 (MQ=25)
gAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa < 1:2296850/71‑1 (MQ=21)
gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacgc < 1:2962188/60‑1 (MQ=9)
gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:1055654/1‑66 (MQ=14)
gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:2463011/1‑66 (MQ=14)
cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc > 1:724456/1‑70 (MQ=14)
aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc > 1:1505333/1‑69 (MQ=14)
tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCCg < 1:2900015/60‑1 (MQ=11)
gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa > 1:2133072/1‑69 (MQ=255)
aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg > 1:3000764/1‑71 (MQ=255)
aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg > 1:1144402/1‑71 (MQ=255)
gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:823804/71‑1 (MQ=255)
gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:2661915/71‑1 (MQ=255)
cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt > 1:179815/1‑71 (MQ=255)
cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt > 1:2153622/1‑71 (MQ=255)
aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:1138976/1‑71 (MQ=255)
ttGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa > 1:343838/1‑59 (MQ=255)
tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg < 1:2921944/53‑1 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa > 1:2569603/1‑53 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:1219709/1‑71 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgag > 1:325411/1‑69 (MQ=255)
gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAACGCCGAGCCAGAACAACAGGTCGATCcgcg > 1:1582045/1‑71 (MQ=14)
ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc > 1:1501657/1‑70 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:1242004/1‑67 (MQ=255)
cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:535065/1‑67 (MQ=255)
cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa < 1:2477368/45‑1 (MQ=255)
aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa < 1:180829/48‑1 (MQ=255)
aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc < 1:2757856/71‑1 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:1251531/1‑45 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:1908143/1‑45 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:637381/1‑45 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcggcc > 1:2475988/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc > 1:122425/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc > 1:1963241/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc > 1:1019973/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc > 1:1730545/1‑71 (MQ=255)
aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc > 1:2762262/1‑70 (MQ=255)
|
GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC > minE/1036437‑1036571
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
