Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 159.3
/ NA
| 48 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (7/41); total (7/41) |
TCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCG > W3110S.gb/4563542‑4563633
|
tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc > 1:1282455/1‑62 (MQ=255)
tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc > 1:1949985/1‑62 (MQ=255)
tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc > 1:29750/1‑62 (MQ=255)
tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc > 1:1241389/1‑62 (MQ=255)
tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc > 1:429122/1‑62 (MQ=255)
tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc > 1:730926/1‑62 (MQ=255)
tCGGCACATAGCAGTTTTCCAGCAGGACATAAATAGGCTGTAACGCCATTTTACTGTCGccc > 1:1073030/1‑62 (MQ=255)
cagcagGTCAT‑AATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:2097723/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTGACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1860269/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:2665166/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:78430/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1999295/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:2324813/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:2487021/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:2615075/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:2646442/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:2910064/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:877987/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:768520/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:742818/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:59363/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:626963/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1891629/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1943169/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1011598/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1622175/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1107714/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1565341/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1386547/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1110615/61‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1193756/61‑1 (MQ=255)
gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1268335/60‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:1008227/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:1073926/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:1078641/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:662358/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:576038/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:44486/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:1305325/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:2804454/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:1601476/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:2435480/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:2305178/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:2281184/62‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:1741031/62‑1 (MQ=255)
cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:2897819/44‑1 (MQ=255)
gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:1339191/53‑1 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1115254/39‑1 (MQ=255)
|
TCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCG > W3110S.gb/4563542‑4563633
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
