Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F3 I0 R1
|
43 |
41.2 |
3165159 |
98.4% |
3114516 |
61.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
100% |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 143.2
/ NA
| 46 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (20/26); total (20/26) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGC > W3110S.gb/4563522‑4563652
|
ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:2490577/71‑1 (MQ=255)
ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:1572656/71‑1 (MQ=255)
agcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTc > 1:630425/1‑71 (MQ=255)
agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:246753/1‑71 (MQ=255)
gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg < 1:3160959/70‑1 (MQ=255)
gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg < 1:1922332/70‑1 (MQ=255)
gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:2672949/69‑1 (MQ=255)
gATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg > 1:2901671/1‑70 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:2848447/54‑1 (MQ=255)
ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:903063/70‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt > 1:2222173/1‑59 (MQ=255)
caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:1472496/1‑70 (MQ=255)
caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTg < 1:3104079/71‑1 (MQ=255)
aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:1587147/44‑1 (MQ=255)
aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:657211/1‑71 (MQ=255)
tCGCAGTTTTCCAGCATGTCATAAATAGGCTGTAACGCCatttt < 1:2753992/44‑1 (MQ=38)
tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg < 1:1227439/51‑1 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:1228040/1‑43 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:390232/1‑45 (MQ=255)
aGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa < 1:2014612/71‑1 (MQ=255)
aGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa < 1:2762726/71‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:2086026/38‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:2808673/59‑1 (MQ=255)
tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac > 1:2325003/1‑56 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:75042/1‑38 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:524797/1‑60 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:2227126/1‑71 (MQ=255)
tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt < 1:555436/70‑1 (MQ=255)
ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt < 1:2292794/70‑1 (MQ=255)
ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa > 1:516292/1‑71 (MQ=255)
gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg < 1:2321562/71‑1 (MQ=255)
gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa < 1:2511927/71‑1 (MQ=255)
tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:2174008/46‑1 (MQ=255)
tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:1261517/46‑1 (MQ=255)
tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg < 1:939674/71‑1 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg > 1:1275843/1‑38 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:3099674/1‑70 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc < 1:2234274/54‑1 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:2191511/71‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa < 1:1569225/71‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa < 1:1399458/71‑1 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:1855675/1‑39 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg < 1:1867573/50‑1 (MQ=255)
ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:1028696/71‑1 (MQ=255)
ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:1027106/71‑1 (MQ=255)
gCTGTAACGCCATTTTACTGGCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:3109151/70‑1 (MQ=255)
cTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:548594/1‑35 (MQ=255)
tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa < 1:2168160/61‑1 (MQ=255)
tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc > 1:2456969/1‑71 (MQ=255)
aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:1837629/1‑61 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAcc > 1:2335200/1‑61 (MQ=255)
|
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGC > W3110S.gb/4563522‑4563652
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
