Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R1
|
87 |
56.5 |
4283937 |
98.6% |
4223961 |
62.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
100% |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 156.2
/ NA
| 53 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (27/26); total (27/26) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA > W3110S.gb/4563522‑4563656
|
ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:350991/71‑1 (MQ=255)
gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:623521/71‑1 (MQ=255)
tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:3983118/71‑1 (MQ=255)
tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:4205460/71‑1 (MQ=255)
cGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:2353913/1‑71 (MQ=255)
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt < 1:1527481/71‑1 (MQ=255)
agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:941885/1‑71 (MQ=255)
agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc < 1:3114842/71‑1 (MQ=255)
agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc < 1:1846395/71‑1 (MQ=255)
cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:3958929/71‑1 (MQ=255)
tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt > 1:381449/1‑58 (MQ=255)
gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg < 1:1349911/70‑1 (MQ=255)
cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt > 1:1593073/1‑70 (MQ=255)
tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg > 1:540016/1‑70 (MQ=255)
gATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:3792057/1‑56 (MQ=255)
gATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:3516193/1‑56 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:1422774/69‑1 (MQ=255)
ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:1966258/71‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:3536660/71‑1 (MQ=255)
caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTg < 1:931641/71‑1 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:1329684/1‑71 (MQ=255)
cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTc > 1:2380212/1‑47 (MQ=255)
cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:2375479/1‑70 (MQ=255)
aGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt < 1:1635391/54‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:695097/1‑70 (MQ=255)
tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg > 1:3979028/1‑60 (MQ=255)
tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt > 1:1957500/1‑71 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg > 1:1112306/1‑59 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt < 1:490527/70‑1 (MQ=255)
cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:3484800/1‑71 (MQ=255)
gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg > 1:2938993/1‑71 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc < 1:461813/71‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc < 1:447999/71‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc < 1:477451/71‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgca < 1:86172/71‑2 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgc > 1:114261/1‑70 (MQ=255)
ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:3329728/52‑1 (MQ=255)
ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:1281930/71‑1 (MQ=255)
ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:2811536/71‑1 (MQ=255)
gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:3398383/1‑53 (MQ=255)
gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc < 1:948681/57‑1 (MQ=255)
gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc < 1:2874819/57‑1 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:2975988/1‑45 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:3403876/40‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:791268/1‑61 (MQ=255)
aTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAcc < 1:660110/71‑1 (MQ=255)
aTAGGCTGAAACGCCATTTTACTGTCGCCCATGTGGAACACgg > 1:2864818/1‑43 (MQ=38)
ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:3272345/71‑1 (MQ=255)
ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:362636/71‑1 (MQ=255)
gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:662133/1‑66 (MQ=255)
gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:903761/1‑66 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa > 1:2618166/1‑59 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa > 1:3885103/1‑59 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:4017671/1‑60 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCggg > 1:1811603/1‑70 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa > 1:1545609/1‑71 (MQ=255)
|
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA > W3110S.gb/4563522‑4563656
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
