Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I0 R1
|
44 |
27.8 |
2109400 |
97.8% |
2062993 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
100% |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 87.1
/ NA
| 29 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/18); total (11/18) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCA > W3110S.gb/4563523‑4563648
|
gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt > 1:294758/1‑70 (MQ=255)
tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:279512/71‑1 (MQ=255)
agcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTc > 1:1783469/1‑71 (MQ=255)
gcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg > 1:911697/1‑71 (MQ=255)
cagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGc > 1:1735583/1‑71 (MQ=255)
cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:1799207/71‑1 (MQ=255)
tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg > 1:1994854/1‑71 (MQ=255)
tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg > 1:1626630/1‑71 (MQ=255)
tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:1051366/55‑1 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:456476/69‑1 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:635387/1‑71 (MQ=255)
ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGACCATGTGGAacac > 1:1052783/1‑71 (MQ=255)
ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGACCATGTGGAacac > 1:1055256/1‑71 (MQ=255)
cacaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg < 1:1255052/71‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:734054/71‑1 (MQ=255)
caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt > 1:879205/1‑45 (MQ=255)
tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:691969/60‑1 (MQ=255)
gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:1891470/38‑1 (MQ=255)
gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg < 1:1484497/71‑1 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt < 1:1063112/70‑1 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCAATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:1267304/1‑71 (MQ=255)
tCCAGCAGGTCATAAATAGGCTTTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt < 1:1990658/70‑1 (MQ=255)
cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:681916/57‑1 (MQ=255)
agcagGTCATAAATAGGCTGTAACGCCATTTTACt < 1:2052912/35‑1 (MQ=255)
gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg > 1:235163/1‑71 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATTTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:831201/71‑1 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:1190199/71‑1 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:864229/71‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa < 1:1570629/71‑1 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa < 1:223987/70‑1 (MQ=255)
tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:476470/1‑51 (MQ=255)
gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCa < 1:1137827/71‑1 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:1959279/53‑1 (MQ=255)
|
GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCA > W3110S.gb/4563523‑4563648
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
