breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 70,434 C→T 77.4% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator
RA 70,581 C→T 75.0% V65V (GTC→GTT araC → DNA‑binding transcriptional dual regulator
RA 70,740 T→G 85.3% G118G (GGT→GGG araC → DNA‑binding transcriptional dual regulator
RA 71,079 C→T 81.4% R231R (CGC→CGT araC → DNA‑binding transcriptional dual regulator
RA 71,082 T→C 81.0% I232I (ATT→ATC araC → DNA‑binding transcriptional dual regulator
RA 71,085 T→C 80.8% S233S (AGT→AGC araC → DNA‑binding transcriptional dual regulator
RA 71,109 T→C 78.0% T241T (ACT→ACC araC → DNA‑binding transcriptional dual regulator
RA 71,175 A→G 82.4% R263R (CGA→CGG araC → DNA‑binding transcriptional dual regulator
RA 71,214 T→C 80.8% F276F (TTT→TTC araC → DNA‑binding transcriptional dual regulator
RA 155,463 A→T 100% *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone
RA 219,687 A→T 14.3% A103A (GCT→GCA rcsF ← predicted outer membrane protein, signal
RA 236,101 A→C 74.8% D12A (GAT→GCT)  dnaQ → DNA polymerase III epsilon subunit
RA 236,107 A→C 78.0% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit
RA 547,694 A→G 100% pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
MC JC 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]
RA 1,093,686 T→C 100% V130A (GTA→GCA)  ycdT → predicted diguanylate cyclase
RA 1,184,032 G→A 16.7% Y125Y (TAC→TAT potD ← polyamine transporter subunit
RA 1,184,053 A→C 16.1% P118P (CCT→CCG potD ← polyamine transporter subunit
MC JC 1,301,041 Δ1,336 bp 100% insCinsD insC, insD
RA 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit
RA 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit
MC JC 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]
MC JC 2,320,795 Δ1,336 bp 100% insD[rcsC] insD, insC, [rcsC]
RA 2,809,581 G→A 79.8% G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator
RA 2,866,110 T→A 100% intergenic (‑20/+160) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
MC JC 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]
MC JC 3,742,938 Δ1,336 bp 100% insDinsC insD, insC
MC JC 3,746,913 Δ1,199 bp 100% [tnaB][tnaB] [tnaB], insH, [tnaB]
MC JC 3,750,593 Δ1,199 bp 100% insH insH
RA 3,824,280 T→C 100% pseudogene (413/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,326 T→C 100% pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,362 A→T 100% pseudogene (495/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,374 C→T 100% pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,380 2 bp→AA 100% pseudogene (513‑514/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,410 T→A 100% pseudogene (543/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,514 T→C 100% pseudogene (647/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,524 T→G 100% pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,533:1 +G 100% pseudogene (666/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
MC JC 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]
RA 4,371,273 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,563,589 T→A 100% K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 573859–575008 575999 992–2141 5 [3] [0] 44 insH–[nmpC] insH,[nmpC]
* * ÷ W3110S.gb 907991 908010 20 4 [3] [3] 4 ybjT conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
* * ÷ W3110S.gb 1173190 1173208 19 4 [3] [3] 4 mfd transcription‑repair coupling factor
* * ÷ W3110S.gb 1328313 1328336 24 4 [3] [3] 4 yciQ predicted inner membrane protein
* * ÷ W3110S.gb 1828084 1828124 41 4 [2] [3] 5 astE succinylglutamate desuccinylase
* * ÷ W3110S.gb 2041778 2041800 23 5 [3] [3] 4 yedY predicted reductase
* * ÷ W3110S.gb 2569112 2569133 22 5 [3] [3] 4 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ W3110S.gb 2777796 2777828 33 5 [3] [3] 4 ypjA adhesin‑like autotransporter
* * ÷ W3110S.gb 2827379 2827422 44 4 [3] [3] 7 gutM DNA‑binding transcriptional activator
* * ÷ W3110S.gb 3044667 3044716 50 4 [3] [3] 4 ygfF/gcvP predicted NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
* * ÷ W3110S.gb 3799043 3799077 35 4 [3] [3] 4 yicM predicted transporter
* * ÷ W3110S.gb 3859252 3859284 33 4 [3] [3] 4 yibK/lldD predicted rRNA methylase/L‑lactate dehydrogenase, FMN‑linked
* * ÷ W3110S.gb 4135552 4135608 57 5 [3] [2] 4 yhfS/frlR conserved hypothetical protein/predicted DNA‑binding transcriptional regulator
* * ÷ W3110S.gb 4213633–4215889 4216706–4215891 3–3074 4 [3] [2] 4 rrlE–[rrfE] rrlE,[rrfE]
* * ÷ W3110S.gb 4401124 4401179 56 5 [3] [3] 5 amiB N‑acetylmuramoyl‑l‑alanine amidase II

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 70061 =20 (0.880)83 (4.650)
+CGCGTACAACT
50/80 NT 80.9% intergenic (‑13/‑326) araB/araC L‑ribulokinase/DNA‑binding transcriptional dual regulator
?W3110S.gb 236044 = 30 (1.320)intergenic (‑42/‑23) rnhA/dnaQ ribonuclease HI, degrades RNA of DNA‑RNA hybrids/DNA polymerase III epsilon subunit
* ? W3110S.gb = 7126519 (0.830)32 (1.940)
+AGGGTCCCTAAGTC
23/74 NT 77.2% coding (879/879 nt) araC DNA‑binding transcriptional dual regulator
?W3110S.gb 3464879 = 7 (0.310)intergenic (‑255/+46) murB/rrfB UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding/5S ribosomal RNA
* ? W3110S.gb 97398 =38 (1.670)3 (0.140) 3/98 NT 9.3% coding (312/1317 nt) murD UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
?W3110S.gb 97426 = 22 (1.010)coding (340/1317 nt) murD UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
* ? W3110S.gb 225154 =NA (NA)3 (0.150) 3/92 NT NA noncoding (1384/1542 nt) rrsH 16S ribosomal RNA
?W3110S.gb 225176 = NA (NA)noncoding (1406/1542 nt) rrsH 16S ribosomal RNA
* ? W3110S.gb = 225915NA (NA)8 (0.420) 5/86 NT NA noncoding (157/2904 nt) rrlH 23S ribosomal RNA
?W3110S.gb = 225926 NA (NA)noncoding (168/2904 nt) rrlH 23S ribosomal RNA
* ? W3110S.gb = 23679823 (1.010)43 (4.010)
+27 bp
27/48 NT 78.2% coding (732/732 nt) dnaQ DNA polymerase III epsilon subunit
?W3110S.gb 4124504 = 28 (1.230)intergenic (+106/‑1) damX/dam hypothetical protein/DNA adenine methylase
* ? W3110S.gb 244046 =17 (0.750)3 (0.130) 3/100 NT 13.1% coding (504/579 nt) lpcA D‑sedoheptulose 7‑phosphate isomerase
?W3110S.gb 244098 = 23 (1.030)coding (556/579 nt) lpcA D‑sedoheptulose 7‑phosphate isomerase
* ? W3110S.gb = 274373NA (NA)18 (0.790) 15/102 NT 100% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/predicted S‑methylmethionine transporter
?W3110S.gb 576000 = 0 (0.000)pseudogene (109/1128 nt) nmpC DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
* ? W3110S.gb 314453 =13 (0.570)14 (0.640)
+TC
13/98 NT 67.5% coding (873/888 nt) eaeH attaching and effacing protein, pathogenesis factor
?W3110S.gb 3932930 = 1 (0.040)coding (219/1608 nt) dppA dipeptide transporter
* ? W3110S.gb 315232 =NA (NA)3 (0.150) 3/92 NT NA coding (422/867 nt) insF IS3 element protein InsF
?W3110S.gb 315255 = NA (NA)coding (445/867 nt) insF IS3 element protein InsF
* ? W3110S.gb = 315707NA (NA)24 (1.050) 19/102 NT 100% coding (654/687 nt) ykgA predicted DNA‑binding transcriptional regulator
?W3110S.gb 576911 = 0 (0.000)coding (76/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 468217 =21 (0.920)4 (0.200) 3/90 NT 18.0% coding (123/1773 nt) mdlA fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily
?W3110S.gb 468244 = 18 (0.900)coding (150/1773 nt) mdlA fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily
* ? W3110S.gb 566000 =NA (NA)33 (1.450) 24/102 NT 100% intergenic (+90/‑56) ybcD/insE DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment/IS3 element protein InsE
?W3110S.gb = 576913 0 (0.000)coding (78/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 713380 =20 (0.880)69 (3.290) 47/94 NT 81.4% intergenic (‑156/‑29) ybfF/seqA conserved hypothetical protein/regulatory protein for replication initiation
?W3110S.gb = 4125340 13 (0.620)coding (836/837 nt) dam DNA adenine methylase
* ? W3110S.gb = 71395434 (1.490)86 (3.770) 51/102 NT 75.4% coding (546/546 nt) seqA regulatory protein for replication initiation
?W3110S.gb 2809398 = 22 (0.970)intergenic (+63/‑28) ygaH/mprA predicted inner membrane protein/DNA‑binding transcriptional regulator
* ? W3110S.gb 894129 =13 (0.570)3 (0.150) 3/88 NT 20.5% intergenic (+274/‑77) ybjN/potF predicted oxidoreductase/putrescine transporter subunit
?W3110S.gb 894150 = 12 (0.610)intergenic (+295/‑56) ybjN/potF predicted oxidoreductase/putrescine transporter subunit
* ? W3110S.gb 1209367 =6 (0.260)10 (0.640) 9/70 NT 58.6% coding (290/630 nt) ycfK hypothetical protein
?W3110S.gb 1211196 = 10 (0.640)pseudogene (37/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 12093827 (0.310)9 (0.580) 9/70 NT 54.9% coding (305/630 nt) ycfK hypothetical protein
?W3110S.gb = 1211179 10 (0.640)pseudogene (54/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 128608620 (0.880)3 (0.150) 3/90 NT 13.4% coding (906/1539 nt) narH nitrate reductase 1, beta (Fe‑S) subunit
?W3110S.gb = 1286104 21 (1.040)coding (924/1539 nt) narH nitrate reductase 1, beta (Fe‑S) subunit
* ? W3110S.gb = 131124517 (0.750)3 (0.150) 3/88 NT 18.4% coding (739/1254 nt) kch voltage‑gated potassium channel
?W3110S.gb = 1311272 12 (0.610)coding (712/1254 nt) kch voltage‑gated potassium channel
* ? W3110S.gb 1324223 =14 (0.610)4 (0.200) 3/88 NT 27.5% coding (438/1563 nt) trpE component I of anthranilate synthase
?W3110S.gb 1324247 = 9 (0.460)coding (414/1563 nt) trpE component I of anthranilate synthase
* ? W3110S.gb = 149941331 (1.360)3 (0.150) 3/92 NT 9.5% coding (814/1626 nt) mdoD glucan biosynthesis protein, periplasmic
?W3110S.gb = 1499426 29 (1.410)coding (827/1626 nt) mdoD glucan biosynthesis protein, periplasmic
* ? W3110S.gb 1883832 =24 (1.050)3 (0.150) 3/92 NT 15.5% coding (207/1086 nt) yeaU predicted dehydrogenase
?W3110S.gb 1883858 = 11 (0.540)coding (233/1086 nt) yeaU predicted dehydrogenase
* ? W3110S.gb 2018571 =1 (0.040)16 (0.750) 15/96 NT 94.4% coding (567/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/predicted transposase
* ? W3110S.gb = 20185832 (0.090)23 (1.050) 16/98 NT 92.3% coding (579/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb = 2521059 NA (NA)intergenic (+170/+30) insL/yfeA predicted transposase/predicted diguanylate cyclase
* ? W3110S.gb 2172308 =NA (NA)25 (1.120) 20/100 NT 100% pseudogene (1563/2041 nt) gatR DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
?W3110S.gb = 3920281 0 (0.000)coding (88/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2173564NA (NA)17 (0.760) 12/100 NT 100% pseudogene (307/2041 nt)
pseudogene (307/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb 3920278 = 0 (0.000)coding (91/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2173565NA (NA)29 (1.270) 23/102 NT 96.7% pseudogene (306/2041 nt)
pseudogene (306/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb = 3932933 1 (0.040)coding (222/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 230464016 (0.700)3 (0.150) 3/92 NT 15.2% coding (1448/2487 nt) napA nitrate reductase, periplasmic, large subunit
?W3110S.gb = 2304649 19 (0.920)coding (1439/2487 nt) napA nitrate reductase, periplasmic, large subunit
* ? W3110S.gb = 2412225NA (NA)31 (1.360) 25/102 NT 93.9% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/predicted aminotransferase
?W3110S.gb = 3748807 2 (0.090)coding (176/984 nt) tnaB tryptophan transporter of low affinity
* ? W3110S.gb = 309894930 (1.320)3 (0.150) 3/92 NT 10.0% coding (436/1047 nt) ansB periplasmic L‑asparaginase II
?W3110S.gb = 3098965 27 (1.310)coding (420/1047 nt) ansB periplasmic L‑asparaginase II
* ? W3110S.gb 3265071 =35 (1.540)3 (0.150) 3/92 NT 9.3% coding (813/990 nt) tdcB catabolic threonine dehydratase, PLP‑dependent
?W3110S.gb 3265090 = 27 (1.310)coding (794/990 nt) tdcB catabolic threonine dehydratase, PLP‑dependent
* ? W3110S.gb 3748799 =1 (0.040)24 (1.050) 21/102 NT 96.0% coding (184/984 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 4056222 = NA (NA)intergenic (‑564/+15) yrhB/insB hypothetical protein/IS1 transposase InsAB'
* ? W3110S.gb 4212589 =NA (NA)3 (0.140) 3/94 NT NA noncoding (934/1542 nt) rrsE 16S ribosomal RNA
?W3110S.gb 4212609 = NA (NA)noncoding (954/1542 nt) rrsE 16S ribosomal RNA
* ? W3110S.gb 4292798 =25 (1.100)3 (0.150) 3/88 NT 13.8% intergenic (+6/‑39) nrfA/nrfB nitrite reductase, formate‑dependent, cytochrome/nitrite reductase, formate‑dependent, penta‑heme cytochrome c
?W3110S.gb 4292820 = 16 (0.810)intergenic (+28/‑17) nrfA/nrfB nitrite reductase, formate‑dependent, cytochrome/nitrite reductase, formate‑dependent, penta‑heme cytochrome c
* ? W3110S.gb 4480518 =28 (1.230)4 (0.190) 3/96 NT 11.7% coding (400/1815 nt) yjgL hypothetical protein
?W3110S.gb 4480541 = 34 (1.590)coding (423/1815 nt) yjgL hypothetical protein
* ? W3110S.gb 4547364 =14 (0.610)4 (0.210) 4/84 NT 21.9% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb 4547678 = 17 (0.910)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin