Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 75.4
/ NA
| 28 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (0/28); total (0/28) |
AGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGG > W3110S.gb/4563565‑4563626
|
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:314552/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:8897/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:843936/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:773121/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:627207/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:51079/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:484692/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:381811/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:355311/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:324896/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:203924/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:183198/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1563297/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1378738/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1365478/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1274310/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1273076/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1215537/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1191602/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1143400/62‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1026849/62‑1 (MQ=255)
agGGCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:462997/62‑1 (MQ=255)
ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1399517/61‑1 (MQ=255)
ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:852452/61‑1 (MQ=255)
gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1008541/60‑1 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:669841/57‑1 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1531837/47‑1 (MQ=255)
gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:400243/46‑1 (MQ=255)
|
AGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGG > W3110S.gb/4563565‑4563626
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
