Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F17 I0 R2
|
162 |
60.6 |
4616657 |
98.5% |
4547407 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
100% |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 178.2
/ NA
| 59 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (30/29); total (30/29) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563522‑4563654
|
ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:199030/71‑1 (MQ=255)
ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:4266933/71‑1 (MQ=255)
gtaggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:1426843/68‑1 (MQ=255)
gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:3684262/71‑1 (MQ=255)
tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:2222115/71‑1 (MQ=255)
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc < 1:2908896/70‑1 (MQ=255)
cagcagCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGt > 1:4494414/1‑71 (MQ=255)
gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:2838317/1‑70 (MQ=255)
cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc > 1:676889/1‑70 (MQ=255)
gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt > 1:865899/1‑71 (MQ=255)
cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:891339/55‑1 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:1971273/53‑1 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg > 1:2789754/1‑68 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg > 1:3148754/1‑68 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa < 1:2836812/71‑1 (MQ=255)
cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:3117901/1‑71 (MQ=255)
cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:327546/1‑71 (MQ=255)
cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:862796/1‑71 (MQ=255)
cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:3180743/1‑71 (MQ=255)
ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg > 1:1262044/1‑66 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:2058666/1‑71 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:1752898/71‑1 (MQ=255)
aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:3635989/1‑63 (MQ=255)
tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:692819/1‑53 (MQ=255)
tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGTTGAc < 1:2211458/71‑1 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg < 1:3443822/57‑1 (MQ=255)
cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:3243068/1‑70 (MQ=255)
aGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa < 1:1672440/71‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc < 1:2136953/40‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc < 1:1018004/40‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg < 1:3263267/53‑1 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg > 1:339434/1‑59 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:97542/1‑68 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:2257499/1‑71 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGt < 1:1523504/70‑1 (MQ=255)
tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt < 1:4046253/70‑1 (MQ=255)
ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt < 1:2883340/70‑1 (MQ=255)
cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa < 1:2487251/70‑1 (MQ=255)
cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc < 1:2377061/71‑1 (MQ=255)
cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgc > 1:4353286/1‑71 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc < 1:4013018/71‑1 (MQ=255)
ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:1349257/39‑1 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt > 1:4178144/1‑37 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:793561/1‑70 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc > 1:2930223/1‑70 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:1259729/71‑1 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg < 1:3269300/54‑1 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:2843663/1‑42 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:1034177/1‑47 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGCCGCCAGGTTTACCGCCGAGCAAAc > 1:2794532/1‑71 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa < 1:1335121/70‑1 (MQ=255)
aaTAGGCGGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc < 1:2484094/71‑1 (MQ=255)
ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCTCCGAGCAAACCGcc < 1:1299799/71‑1 (MQ=255)
ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:2623948/71‑1 (MQ=255)
ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:867430/71‑1 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt < 1:3437929/50‑1 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc > 1:4282352/1‑71 (MQ=255)
gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:3906858/67‑1 (MQ=255)
tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:3439726/1‑38 (MQ=255)
tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:4115695/45‑1 (MQ=255)
tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc > 1:1582778/1‑71 (MQ=255)
tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc > 1:2410313/1‑71 (MQ=255)
tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc > 1:3409162/1‑71 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc < 1:2489404/60‑1 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg < 1:1160938/71‑1 (MQ=255)
|
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563522‑4563654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
