Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 87.7
/ NA
| 29 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (29/0); total (29/0) |
CAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCG > W3110S.gb/4563564‑4563636
|
cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:86054/1‑62 (MQ=255)
cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:1023715/1‑62 (MQ=255)
cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:2763051/1‑62 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg > 1:2866086/1‑61 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg > 1:883596/1‑61 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg > 1:724893/1‑61 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg > 1:531968/1‑61 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg > 1:2088842/1‑61 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg > 1:2038835/1‑61 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg > 1:1570301/1‑61 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg > 1:1165919/1‑61 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg > 1:564017/1‑37 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:1094315/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:1915396/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:110492/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:790763/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:756970/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:745374/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:639250/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:624021/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:579731/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:1437500/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:377593/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:3053138/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:2899186/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:1519205/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:258823/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:2203953/1‑38 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:1698142/1‑38 (MQ=255)
|
CAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCG > W3110S.gb/4563564‑4563636
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
