Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F17 I0 R1 87 120.7 2441348 95.3% 2326604 231.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G97.1% 359.0 / 0.7 102intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (61/38);  total (63/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 104 total aligned reads displayed.

TCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  NC_000913/3560219‑3560687
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        
tCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑cgc                                                                                                                                                                                                                                         >  1:758191/1‑237 (MQ=255)
 cAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑cgca                                                                                                                                                                                                                                        >  1:366684/1‑236 (MQ=255)
               cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGtttt                                                                                                                                                                                                                            >  1:169936/1‑237 (MQ=255)
                gTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGAGCAGGTTTTGc                                                                                                                                                                                                                          >  2:349451/1‑238 (MQ=255)
                      aTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCa                                                                                                                                                                                                                   >  1:37853/1‑239 (MQ=255)
                       ttgttgGTGAAAATGCGCAAATGGCTGTGATTGAGCAGGGGGTGCGCTCCCGCTCCCGGCGTGGTGCCGATATCGATAAACAGCGGCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                                                                                                                                  <  1:89404/239‑1 (MQ=255)
                         gttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                 >  1:1102513/1‑238 (MQ=255)
                         gttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                 >  1:368126/1‑238 (MQ=255)
                          ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGGCTGCGCGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGACGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTCCGccgcccgcc                                                                                                                                                                                                                >  2:531551/1‑237 (MQ=255)
                            ggTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGAG‑CAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                             >  1:996507/1‑239 (MQ=255)
                                    tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGTGGGCAGCTCCGCACCGCCATGA‑T‑GG‑CGCAGGGTCAGCGTTTGCGCCGCAAGCACATTGAGGTc                                                                                                                                                                                                      >  2:517775/1‑238 (MQ=255)
                                    tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCCTGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAggcc                                                                                                                                                                                                      >  1:555267/1‑236 (MQ=255)
                                    tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                      >  1:190136/1‑238 (MQ=255)
                                    tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                      >  1:24133/1‑238 (MQ=255)
                                    tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTAAGGTc                                                                                                                                                                                                      >  2:990207/1‑238 (MQ=255)
                                                tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGCAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCCGGTTTTGCTCCGCCAGCTCATTTCGGTCGCGGCGAATAg                                                                                                                                                                                           >  2:111792/1‑237 (MQ=255)
                                                   gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCGCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                      >  1:717102/1‑239 (MQ=255)
                                                   gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                      >  1:1029354/1‑239 (MQ=255)
                                                   gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                      >  2:8120/1‑239 (MQ=255)
                                                   gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCCCCAGCTCATTGGGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                      >  2:504555/1‑239 (MQ=255)
                                                       gAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                    >  1:714680/1‑237 (MQ=255)
                                                         gCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCt                                                                                                                                                                                 >  1:510016/1‑238 (MQ=255)
                                                            gtgcgtgcGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                             >  2:83695/1‑239 (MQ=255)
                                                            gtgcgtgcGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                             >  1:906536/1‑239 (MQ=255)
                                                             tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGCATAGTCTGCGGGCTGCCgg                                                                                                                                                                            >  2:491870/1‑239 (MQ=255)
                                                                gggcggccccGCTCGCGGCGGGTTGCCGTGTTGGATAAACGGCGTGGGGCCA‑TGGGGGATTGGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGACCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                                          <  2:758191/230‑1 (MQ=255)
                                                                  gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa                                                                                                                                                                       >  1:1018321/1‑239 (MQ=255)
                                                                  gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa                                                                                                                                                                       >  2:663664/1‑239 (MQ=255)
                                                                  gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa                                                                                                                                                                       >  1:966756/1‑239 (MQ=255)
                                                                    gcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGAGAATAGGATGCGGGCTGAGGGAGAAAt                                                                                                                                                                      >  2:868821/1‑238 (MQ=255)
                                                                       aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCCCCGCCGTGA‑T‑GG‑CGCAGGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCTGGCGGAGAAATGc                                                                                                                                                                    >  1:5295/1‑237 (MQ=255)
                                                                       aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                                                                                                                    >  2:1089382/1‑237 (MQ=255)
                                                                       aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTATTCGGTCTGGGTGGACTTGCGATCGTGCCACGGCGTGTT‑AACAGAACTGGAAGGCGGCCCCGCACCGCCATGA‑TGGG‑G‑CAGGATCCGGTTTTGCTCAGCCAGACCCTTGAGGTCGCGGCGCTTAATCTGCGTGCTGCCGGAGACATGc                                                                                                                                                                    >  2:380655/1‑237 (MQ=255)
                                                                        tcgtcaccGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGCAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCACTGgtct                                                                                                                                                                 >  1:1067344/7‑239 (MQ=255)
                                                                             tCCGGCGGGGTGCTGATATGGTAAAACAGGGTCGAGCCT‑TTGGGGTTTTGCTCCGCCACTTGGCGGGGGATGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGAGCGTGCCACGGGGTGTT‑AACGGAACTGGAAGGCGGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                            <  2:1190001/239‑1 (MQ=255)
                                                                                  cGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGCAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGTCGGAGACATGCTCTACCAGctct                                                                                                                                                        >  2:534977/1‑238 (MQ=255)
                                                                                  cGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGctct                                                                                                                                                        >  1:461506/1‑238 (MQ=255)
                                                                                      gtgCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                   >  1:957726/1‑239 (MQ=255)
                                                                                      gtgCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                   >  1:771010/1‑239 (MQ=255)
                                                                                      gtgCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                                                   >  1:10209/1‑239 (MQ=255)
                                                                                        gcggATATCGATAAACAGCGTCG‑GCCATTTGGGGGTTTGCCCCGCCACTTTGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGGGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                  <  1:155336/235‑1 (MQ=255)
                                                                                        gCCGATACTGATAAACAGCTTCGAGCCT‑TGGGGGATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                  <  1:306719/238‑1 (MQ=255)
                                                                                         ccGATATCGATAAACAGGGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                 <  1:686573/238‑1 (MQ=255)
                                                                                          cGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGtcttg                                                                                                                                               >  2:1123104/1‑235 (MQ=255)
                                                                                            atatCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAc                                                                                                                                             >  1:249358/1‑239 (MQ=255)
                                                                                                 gATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCTGTCGGGGAACTGCTCTACCAGCTCTCAGGTACTGACATaa                                                                                                                                         >  2:17850/1‑238 (MQ=255)
                                                                                                    agaccgggccGAGTCA‑TGGGGGTTTTGCTCCGCCCCTGTCGGGGGGATGGGCTCTTTTTCGTCGGTCGGGGGGCCCTTGCGTTCGTGCCGCGGCGGGTT‑AACCGAACTGGAGGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                     <  2:303229/230‑1 (MQ=255)
                                                                                                    aaccggcgcCGGGCCA‑TTGGGGATTTGCGCCCCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                     <  1:111303/234‑1 (MQ=255)
                                                                                                    aaaCAGCGTCGAGCCA‑TTGGGGATTTGCCCCGCCACTGTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                     <  1:306032/239‑1 (MQ=255)
                                                                                                          cGTCGGGCTA‑TGGGGGATTTGCTCCGCCGCTTGGCGGGCGAGGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                                                                                                                                <  2:717102/238‑1 (MQ=255)
                                                                                                          cGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                               >  1:771790/1‑239 (MQ=255)
                                                                                                          cGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                               >  1:680433/1‑239 (MQ=255)
                                                                                                          cGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCCCTTTTTATTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTTGAAAGCAGAGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGCGGTCGCGGCGAACAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGCACTGACATAACCCTGCTGtt                                                                                                                               >  2:1198564/1‑239 (MQ=255)
                                                                                                           gTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGttt                                                                                                                              >  1:595450/1‑239 (MQ=255)
                                                                                                            tCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                                                                                                                                  >  1:745425/1‑170 (MQ=255)
                                                                                                                ggcctgtGGGGGTTTCCGCCGCCGCTTTGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTGGCGAGCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                           <  2:714680/232‑1 (MQ=255)
                                                                                                                gcccgttGGGGTTTGACTCCGCCACTTTGCGGGCGATGCGTTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCGCTGGGGGTTT‑AACCAAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                           <  1:623790/233‑1 (MQ=255)
                                                                                                                 cta‑TTGGGTATTTGCCCCGCCACTTTGCGGGCGATGCGCTTTTTTCCTGCGGCCCGGGTGCCCTTGCGAGCGTCCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                        <  1:941208/237‑1 (MQ=255)
                                                                                                                   ctttGGGGTTTTGCTCCGCCACTTGGCGGGCGATGGTCTTTTTTTCTCGGGTCTGGGTGGCCTTGCGACCGGGCCGCGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                        <  2:37853/236‑1 (MQ=255)
                                                                                                                           gtttGCCCCGCCCCTGTGCGGGCTATGCGCTCTTTTGCTGCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGGGTT‑AACCGAAGTGGAGGGCAGCCCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa                                                                                                                <  2:249358/237‑1 (MQ=255)
                                                                                                                           aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCGCGGCGTGTT‑AACCGAACGGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa                                                                                                                <  1:280599/238‑1 (MQ=255)
                                                                                                                               tgtcgcccttgttGCGGCGGATCCGCTCTTTTTCTTTGGTCTGGTGGGCCTTGCGCTCGTGCTGGGGGGTGTA‑ACCCGAAGTGGAAGGCCGCGCGGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg                                                                                                           <  2:959805/228‑1 (MQ=255)
                                                                                                                               gcgccGCCCTTTTCCGGGCGATGCGCTTTTTTCCTGCGGTCTGGGTGGCCTTGCGGTCGTGCCACGGCGGGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg                                                                                                           <  2:281639/236‑1 (MQ=255)
                                                                                                                               gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg                                                                                                           >  1:540217/1‑239 (MQ=255)
                                                                                                                                 tCCGCCCCTTGGCGGGCGATGGGCCCTTTTTCTTCGGTCTGGGGGGCCTGGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                         <  1:176306/239‑1 (MQ=255)
                                                                                                                                 gccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCATGCCACGGCGTGTT‑AACCGACCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                         <  1:460479/238‑1 (MQ=255)
                                                                                                                                  ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg                                                                                                        >  2:1063180/1‑239 (MQ=255)
                                                                                                                                   gctctcTTCGCGGCCGACGCGTTCTTTTTCTGCGGTCTGGGTGCCCGT‑CGCTCGGTGCTCGGCGTGTTAAACCAATCTGGAAGGCGGCGCCGCTCCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATTCCGTtgt                                                                                                       <  2:1067344/234‑1 (MQ=255)
                                                                                                                                        cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCGGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt                                                                                                  <  1:17850/239‑1 (MQ=255)
                                                                                                                                         tttGCGGGCGATGCGCTCTTTTCCTTCGGTCTGGGTTGCCTTGCGATCTTGCCACGGCGTGTT‑ACCCGAACTGGAAGGCAGCGCCTCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                 <  1:553669/239‑1 (MQ=255)
                                                                                                                                          ttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                >  1:658672/1‑239 (MQ=255)
                                                                                                                                                           tttgtCGTCGGTCGGGGGGGTCTTGGCATCGGGCCACGGCGTGTA‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtata                                                                               <  2:95982/239‑1 (MQ=255)
                                                                                                                                                            tttaCTGCGGTGTGGGTGGCCTTGCGATCGTGCAGCGGCGTGTG‑ACCCGAACGTGAGGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                                              <  2:5295/239‑1 (MQ=255)
                                                                                                                                                                   cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTg                                                                       >  1:874746/1‑239 (MQ=255)
                                                                                                                                                                     gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGTAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGGGGCGAAAAGTCTGCGGGCTGCAGGCGAACAGCTCTACCAGCTCTTCGAGACTGACCTAACCCTGCTGTTTAACCAGTTAGATAATACACTTTTGACGTTGGGTTTGTTTCATTTACAAATACATGGa                                                                     >  2:877682/1‑239 (MQ=255)
                                                                                                                                                                     gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTggg                                                                     >  2:948132/1‑238 (MQ=255)
                                                                                                                                                                     gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGa                                                                     >  1:1207225/1‑239 (MQ=255)
                                                                                                                                                                     gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGa                                                                     >  1:774073/1‑239 (MQ=255)
                                                                                                                                                                     gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGa                                                                     >  1:474514/1‑239 (MQ=255)
                                                                                                                                                                                    cGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                      >  1:156934/1‑239 (MQ=255)
                                                                                                                                                                                    cGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                      >  2:173103/1‑239 (MQ=255)
                                                                                                                                                                                    cGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGCTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                      >  2:491271/1‑239 (MQ=255)
                                                                                                                                                                                         gTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa                                                 >  2:1013400/1‑239 (MQ=255)
                                                                                                                                                                                              aCGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCGCCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                            <  1:572883/239‑1 (MQ=255)
                                                                                                                                                                                              aCGGCGGGTT‑AACGGGACGGGAAGGCAGCGCCGGACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGGCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                            <  1:531551/239‑1 (MQ=255)
                                                                                                                                                                                                    tgtT‑AACCGACCTGGAAGCCGGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                       <  2:1029354/238‑1 (MQ=255)
                                                                                                                                                                                                         aaCCGACCTGGAGGGCAGCGCCCCGCCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca                                  <  1:1152560/239‑1 (MQ=255)
                                                                                                                                                                                                           ccGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                >  2:369721/1‑239 (MQ=255)
                                                                                                                                                                                                              aaCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg                              >  2:60866/1‑238 (MQ=255)
                                                                                                                                                                                                               aCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                            >  2:879394/1‑239 (MQ=255)
                                                                                                                                                                                                                      ggcggcggcGCACCGGCATGAGG‑GG‑G‑CAGGATCAGGTTTTGCACCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                      <  2:245206/238‑1 (MQ=255)
                                                                                                                                                                                                                        cAGCGCCGCACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaa                    >  1:334886/1‑238 (MQ=255)
                                                                                                                                                                                                                           cgccgcACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                            >  2:5337/1‑227 (MQ=255)
                                                                                                                                                                                                                           cgccgcACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                            <  1:5337/227‑1 (MQ=255)
                                                                                                                                                                                                                             ccgcACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                      <  2:300830/231‑1 (MQ=255)
                                                                                                                                                                                                                             ccgcACCGCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                      >  1:300830/1‑231 (MQ=255)
                                                                                                                                                                                                                                    gCCATGA‑T‑GG‑CGCAGGATCAGGTTTTTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAGTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc       <  1:717786/239‑1 (MQ=255)
                                                                                                                                                                                                                                    gCCATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc       <  1:369721/239‑1 (MQ=255)
                                                                                                                                                                                                                                     ccATGA‑T‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGGTAAACCCACGGCTAAc       >  1:905129/1‑238 (MQ=255)
                                                                                                                                                                                                                                         ga‑t‑gG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc  <  1:491271/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        
TCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCA‑TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTT‑AACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  NC_000913/3560219‑3560687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A