Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F17 I0 R1 40 55.2 1410130 81.3% 1146435 215.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G96.4% 74.1 / ‑4.5 27intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (21/5);  total (22/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCC  >  NC_000913/3560229‑3560661
                                                                                                                                                                                                                                    |                                                                                                                                                                                                              
aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCCGCACCGCCAT‑GAGGGcg                                                                                                                                                                                                                >  1:423568/1‑227 (MQ=255)
    aCGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATAGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCCGCACGCCCAT‑GCTGGcgcag                                                                                                                                                                                                             >  1:485732/1‑223 (MQ=255)
       ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGcgccggat                                                                                                                                                                                                          >  1:672036/1‑220 (MQ=255)
                ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCAT‑GATGGCGCAGGAGCGGGtctgg                                                                                                                                                                                                 >  1:98828/1‑223 (MQ=255)
                 tgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCAT‑GATGGCGCAGGATCAGGTTTTGCt                                                                                                                                                                                               >  1:540484/1‑228 (MQ=255)
                              cAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCCTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                  >  1:556367/1‑228 (MQ=255)
                                           ttGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGCAGGCAGGGCCCCCCCGCCATAGTGGGC‑CGGGATAGGGTTTTGCCCCGCCGCCCCCTTGGGGGCGCGGGGa                                                                                                                                                                     >  2:222770/1‑228 (MQ=255)
                                                  gtgcgtgcGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt                                                                                                                                                              >  1:199225/1‑228 (MQ=255)
                                                   tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                                                                                             >  1:691380/1‑228 (MQ=255)
                                                   tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCAACGGCGTGTTAACCGAAGTGGAAAGCAGCGCCGCACCGCCAG‑GATGGCGCAGAATAAGGTTTGGCTCCGCCAGCCCATGTGGGCCGCGGGGAATAGtacg                                                                                                                                                             >  2:201846/1‑225 (MQ=255)
                                                                            gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTTACGGGGAAAtccctgcc                                                                                                                                     >  1:354442/1‑220 (MQ=255)
                                                                                          aaaCGGCGTCCGGCCTTGGGGGATTTGCCCCGCCACGTGGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                       <  1:222770/227‑1 (MQ=255)
                                                                                                  tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCAT‑GATGGCGCGGGATCAGGTTTTGTTCCGCCAGCTCATTGAGGTCGCGGCGAAAAGTCTGCGGGCTGACGGGGCAAGGGCCTACCCGCTCTTCGGGCTTGACATaaa                                                                                                              >  2:423339/1‑227 (MQ=255)
                                                                                                                    gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGACACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GCTGGCGCAGGATCCGGTTTTGATCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGGGGGCTGCCGGGGAAATTCTCTACCAGCTCTCCGGACCTGCCATAACCCCTGTGTTTAACCAAtt                                                                                            >  2:270163/1‑228 (MQ=255)
                                                                                                                     cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTACGGTCGCGGCGATTAGTCTGCGGCCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCGGTTTAACCAGTTc                                                                                           >  1:339024/1‑228 (MQ=255)
                                                                                                                          ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGGGAATAGTCTGCGGCCTGTCGGGGAAATGCTCTACCACCTCACCGGTCCTGACCGAAACCTGCTGTTTAACCCGTTcaagaa                                                                                      >  2:115913/1‑223 (MQ=255)
                                                                                                                               tggCGGGCGATGCGCTGTTTTTCTCGGGTCTGGGTGCCCGTGCGATCGTGCCACGGTGTGTTAGCCGAACTGGAGGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg                                                                                 <  1:115913/226‑1 (MQ=255)
                                                                                                                                               ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAGGCTCTACCGGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCCGTTCGATAATACCGCTGGGACCTTGtgttt                                                                 >  2:442282/1‑228 (MQ=255)
                                                                                                                                               ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGCGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGTTATTACCGTTTTGACGTTGtgttt                                                                 >  1:461646/1‑228 (MQ=255)
                                                                                                                                                   tgcttgGGTCTGGGTGGCCTGGCGATCGGGCCCCGGGGTGTTAACGGAACTGGAAGGCAGCGCCGAACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                              <  1:14363/225‑1 (MQ=255)
                                                                                                                                                               ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGTTGACGGAGAACTGCCCTACCAGCTCTTCGGTACTGCCATAACCCCGCTGTTTAACCAGTTCGCTAATACCCTTGTTACGTATTGTTTTTTTCCTTTTTAAATc                                                 >  2:182105/1‑228 (MQ=255)
                                                                                                                                                               ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTTACATAACCCTGCTGTTTAACCAGTTCGACAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTaaaac                                                 >  1:638548/1‑226 (MQ=255)
                                                                                                                                                                     cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTTCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGGCATAACCCTGCTGTTTAACCAGTTCGGTCATACCGTTGTGACGTTGGTTTTGTTTCATTTATAAATCCCTGGa                                           >  1:654995/1‑228 (MQ=255)
                                                                                                                                                                                         tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGCAATAACCCTGCCGTTTAACCCGTTCGATATACCCCTTTTTACGTTGTGTTTGTTTCCTTTATATATCCCTGGAATTTttttcgttttccggca                       >  2:34552/1‑223 (MQ=255)
                                                                                                                                                                                         tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTTTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgcc                       >  1:444955/1‑227 (MQ=255)
                                                                                                                                                                                                 gAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCCTTGAGCg                >  2:374195/1‑227 (MQ=255)
                                                                                                                                                                                                            cAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAGTTATTTTCGTTTTCGCGCATTGAGCGCATCAACaaaa     >  1:583555/1‑227 (MQ=255)
                                                                                                                                                                                                               cgccgcACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATCGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc  <  1:442282/227‑1 (MQ=255)
                                                                                                                                                                                                               cgccgcACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt                                                                                                                         >  1:403243/1‑108 (MQ=255)
                                                                                                                                                                                                                gccgcACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                        <  2:403243/108‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                              
AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCC  >  NC_000913/3560229‑3560661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A