Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I0 R1 43 55.9 1320120 86.3% 1139263 218.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G90.9% 132.0 / 3.1 44intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base G (27/13);  total (30/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGC  >  NC_000913/3560229‑3560660
                                                                                                                                                                                                                                     |                                                                                                                                                                                                             
aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGcg                                                                                                                                                                                                               >  1:484710/1‑227 (MQ=255)
     cGTTGAGATTGTTGGAGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATGATGGGC‑CAgg                                                                                                                                                                                                           >  1:480044/1‑227 (MQ=255)
       ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCCCACCGCCATGCT‑GGCGCAGGAt                                                                                                                                                                                                         >  1:645334/1‑227 (MQ=255)
       ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGAG‑CTCTTTTTCTCCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCATAAG‑GGcgcgggat                                                                                                                                                                                                         >  1:117386/1‑220 (MQ=255)
               gttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGGG‑CAGGGTCAGGtttt                                                                                                                                                                                                 >  1:328576/1‑227 (MQ=255)
                 tgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCt                                                                                                                                                                                              >  1:617647/1‑228 (MQ=255)
                                                  gtgcgtgcGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTCACCGAACTGGAAGGCAGCGCCCCACCGCCATGATGGGC‑GCGGACCGGGCTTTTCTCCGCCAGACCATTGGGGTGCGGGCGAAAAGGCt                                                                                                                                                             >  2:327882/1‑228 (MQ=255)
                                                   tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATAAGGTTTTGCTCAGCCAGCCCATTCCGGTCGCGGCGACTAGTccg                                                                                                                                                            >  1:582864/1‑226 (MQ=255)
                                                   tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAGGAT‑GGCGCAGGCTCAGGTTTTGGTCCGCCACCTAATTGAGGTGCCGGCGAAAAgccgg                                                                                                                                                            >  1:622009/1‑224 (MQ=255)
                                                   tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAAGCT‑GGCGCAGGAACAGGTTTTGCTCCGCCCGCCCCTTGAGGTCGGGGCGAAtgggacg                                                                                                                                                            >  2:42042/1‑222 (MQ=255)
                                                     cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGCTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGGCAATAGTCTGCg                                                                                                                                                          >  1:408469/1‑228 (MQ=255)
                                                                 cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCACCTCATGTAGGTCGCGGCGAAACGTCTGCGGGCTGACGgaga                                                                                                                                              >  2:291208/1‑228 (MQ=255)
                                                                            gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTAcc                                                                                                                                   >  1:62526/1‑228 (MQ=255)
                                                                                       gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCCCTTCgg                                                                                                                         >  1:576230/1‑227 (MQ=255)
                                                                                       gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAAAGCCTGCGGGCTGACGGAGAAATGCCCTACCAGCCCTTCgg                                                                                                                         >  1:162775/1‑227 (MQ=255)
                                                                                              aGCGTCGAGCCATTGGGAATTTGCTCCGCCATTTGGGGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCAACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                                                                                                 <  1:389218/228‑1 (MQ=255)
                                                                                                cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa                                                                                                               >  1:548871/1‑228 (MQ=255)
                                                                                                cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCCCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                                                                                                 >  1:59018/1‑226 (MQ=255)
                                                                                                cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTCCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATCCCCTACCCCCCCTTCgtgccagaatta                                                                                                               >  2:104273/1‑217 (MQ=255)
                                                                                                 gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                                                                                       >  1:590983/1‑203 (MQ=255)
                                                                                                     ggCCTTTGGGGTTTTCCCCGGCCCGTTTGCGGGCGATGCC‑CTCTTGTCCTGGGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                          <  1:291208/227‑1 (MQ=255)
                                                                                                     ggCCTTTGGGGATTTGCTCCGCCGTTTTGCGGGCGATGCG‑CTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                          <  1:511959/227‑1 (MQ=255)
                                                                                                     aGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGGGAAATGCCCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                          >  1:227269/1‑228 (MQ=255)
                                                                                                       ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGATCTACCAGCTCTTCGGACCTGCCATAACCctgc                                                                                                        >  1:607051/1‑228 (MQ=255)
                                                                                                                  ttcccccGCCCTTTTGGGGGCGGTGCC‑CTTTTTTTCTTCGTGTGGGGGGGCCTGTCGATGGTCCCCGGGGGGTTTAACCGAACTGGAAGGCGGCCCCGCCCCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                             <  2:601170/223‑1 (MQ=255)
                                                                                                                   gtcccggCCAGTTGGCGGGCG‑TGCGCCTCTTTTTGTACGGTCGGGGGGGCTTTGCGATCGTGCCACGGCGTGTAAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                             <  1:104273/221‑1 (MQ=255)
                                                                                                                   gtccccGCCATTTTGGGGGGGATGCG‑TTCTTTTTCTCCGGTCTGGGTGGCTTTGCGATCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCAGCCCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                             <  1:619205/223‑1 (MQ=255)
                                                                                                                    gCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCCCGGCGAAAAGTCTGCGGGCTACCGGAGAAATGCTTTACCAGCTCTTCGGTAATGCCATAACCCTGCATTTAAAccctgt                                                                                           >  2:250359/1‑224 (MQ=255)
                                                                                                                     cTCCGCCTTTTTGCGGGCTCTGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                          <  1:250359/212‑1 (MQ=255)
                                                                                                                          ccACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGAGGGCTGACGGGGAAATGCTCTACCCGCACTTCGGTACTGACATAACCCTGCTGTTTACACCGTTCGgtaa                                                                                     >  1:535698/1‑228 (MQ=255)
                                                                                                                           tttttggggggCGATGCG‑CTTTTTTCCTGGGGGTGGGGGGGCCTTGCTGTGGGGCCCGGGGTTGTTACCCGACTTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa                                                                                     <  2:162775/219‑1 (MQ=255)
                                                                                                                                  ggggcagtGCG‑CTTTTTTTCCGCGGGCGGGGTGGCCTTGCGACCGGGCCGCGGGGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg                                                                             <  1:157973/221‑1 (MQ=255)
                                                                                                                                                      cttcGGTTGGGGGGGCTTTGCGATCGGGCCATGGCGTGTTAACGGAACGGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                           <  1:494449/227‑1 (MQ=255)
                                                                                                                                                           gtgtGGGGGGCTTTGCGTTCGTGCCACGGCGTGTTAACCAAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtat                                                     <  1:327882/225‑1 (MQ=255)
                                                                                                                                                           gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCTGCTCTTCGGTATTGACATAACCCTGCGGTTTAACCCATCCGATAATACCCCTTTGAAGTTTTGTTTGTTTCTTTtat                                                     >  2:596459/1‑228 (MQ=255)
                                                                                                                                                           gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAACGACATAACCCTGCTGTTTACCCAGTTCg                                                                                         >  1:262693/1‑192 (MQ=255)
                                                                                                                                                            tcggggTGGCCTTGCGACCGTGACACGGCTTGTTAACCGAACTGGAAGGCAGCGCCGAACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtata                                                    <  1:87591/225‑1 (MQ=255)
                                                                                                                                                                ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                           >  1:509161/1‑121 (MQ=255)
                                                                                                                                                                     ctgtGCGACCGTGCCGCGGGTTGTTAACCGACCTGGAAGGCAGCCTCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTTGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTgg                                           <  2:442525/225‑1 (MQ=255)
                                                                                                                                                                      cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGTTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGGGTTTGTTTCATTTTTAAATCCCTGGa                                          >  1:466325/1‑228 (MQ=255)
                                                                                                                                                                          cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACTCTGCGTGTTAACCCGTCCGATAATACCGGTGGAACCTTGTGTTGGTTTCATTTATAAATCCCTGGAatta                                      >  2:554889/1‑228 (MQ=255)
                                                                                                                                                                                          tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTTTTTTTGTTTTTCCGCa                      >  2:307346/1‑228 (MQ=255)
                                                                                                                                                                                                    aCTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCGGGATCAGGTTTTGCTCCGCAAGCTCATTGATGTCGCGGCGAACAGTCTGCGGACTGACGGAGAAATGCTCTACGAGCTCTTCGGTACTGACATAACCCTACGGTTTAACCATTTCGATAATACCGTTTGTACGTTGTGTTTGGTTCATTTATAAAACCCTGGAAATTTTTTTGTTTTTGCGACTTTAGAGAAt            >  2:430983/1‑228 (MQ=255)
                                                                                                                                                                                                    aCTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGACTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGATTGTTTCATTTATAAATCCCTGGAATTAGTTTCGTTTTCGCGCATTGTGCGAAt            >  1:358814/1‑228 (MQ=255)
                                                                                                                                                                                                             cAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa    >  2:340412/1‑227 (MQ=255)
                                                                                                                                                                                                              gcccccgcTCCCCCATGGTGGGG‑CAGGATCGGGTTTTGCTCCGCCAGCTCATTGAGGTCGGGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc  <  2:227269/224‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                             
AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCG‑CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGC  >  NC_000913/3560229‑3560660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A