Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F97 I2 R1 17 63.6 2309030 90.9% 2098908 142.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G97.0% 204.5 / ‑1.7 67intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (30/34);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCG  >  NC_000913/3560316‑3560582
                                                                                                                                              |                                                                                                                               
gATAAACAGCGTCGAGCCATTGGGGCTTCGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑cgca                                                                                                                               <  1:313781/143‑4 (MQ=255)
     aCAGCGTCGAGCCATTGGGGATTTGCTCCGACACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATc                                                                                                                          >  2:565741/1‑143 (MQ=255)
        gtgtggggCCATGGGGGATTTGGTCCGCCACTTTGGGGGCGAGGCACTTCGTTGTGTCGGTCTGGGTGGCCGTGCGATCGTGCCGCCGCGTGTTGGCCGGACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAgg                                                                                                                       <  2:456925/136‑1 (MQ=255)
          gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTGAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ACGG‑CGCAGGATCAGGtg                                                                                                                     >  1:171939/1‑142 (MQ=255)
           tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGttt                                                                                                                    >  2:443790/1‑143 (MQ=255)
                ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTc                                                                                                               <  1:1149396/143‑1 (MQ=255)
                     ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCCGTCTGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTACCCGAACTGGGAGGCAGCGCCGCACCGCCGTG‑ATGG‑CGCAAGGTCAGGTTTCGCCCCAccc                                                                                                          >  2:59703/1‑142 (MQ=255)
                      gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                         >  1:349008/1‑143 (MQ=255)
                          tttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCa                                                                                                     >  1:657896/1‑143 (MQ=255)
                              cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                                                                 <  1:947216/143‑1 (MQ=255)
                                 cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGt                                                                                              >  1:221261/1‑143 (MQ=255)
                                     tcggtGCGGGCGATGCGCTCTGTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTGACCGAACTGGACGGCAGCGCCGCACCGCCATG‑GTGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgcg                                                                                          <  2:905674/139‑1 (MQ=255)
                                     aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgcg                                                                                          >  1:1063226/1‑143 (MQ=255)
                                      cTTTGCGGGCGTTGCGCTCCGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                         <  2:485101/143‑1 (MQ=255)
                                          gCGGGCGCTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGACCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                     <  2:743714/143‑1 (MQ=255)
                                           cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGAGCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                    >  1:112625/1‑143 (MQ=255)
                                            gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                   <  1:565741/143‑1 (MQ=255)
                                               cGAGGCGCTATTGTTCTGGCGTCTGGGTGGCCTTGGGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc                                                                                <  2:576695/143‑1 (MQ=255)
                                                  tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAAACA‑A‑AAGAATCAGTTGTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                             >  1:837464/1‑143 (MQ=255)
                                                   gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCCTTGAGGTCGCGGCGAATAGTCTGCg                                                                            >  2:938721/1‑143 (MQ=255)
                                                      ctctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                         >  2:1145233/1‑143 (MQ=255)
                                                      ctctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGGAG‑AAGGATCAGGCGCTGCTCCGCCAGCTCCTTGAGGTCGCGACGAATAGTCTGCGGGc                                                                         >  1:838017/1‑143 (MQ=255)
                                                      ctctGTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                         <  2:849922/143‑1 (MQ=255)
                                                      cgctTTTCCCTCGGTCTGGGTGGCCGTGCGATCGGGCCACGGCGTGTTCCCCGACCTGGAAGGCATCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                         <  1:533336/141‑1 (MQ=255)
                                                      acatctctctgcGGTCTGGGTGTCCTTGCGATCGTGCCACGTCGTGTGATCCGAACTGGAAGGCAGCGCCGCCCCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGATGTCGCGGCGAATAGTCTGCGGGc                                                                         <  2:875393/136‑1 (MQ=255)
                                                           tttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCGGACg                                                                    >  1:26988/1‑143 (MQ=255)
                                                           tttCTTCGGTCGGGGTGGCCTCGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCATGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                    <  1:1069147/143‑1 (MQ=255)
                                                               ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAACAGTCTGCGGGCTGACGgaga                                                                >  2:23634/1‑143 (MQ=255)
                                                                tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                               <  1:1107857/143‑1 (MQ=255)
                                                                tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                               >  2:556747/1‑143 (MQ=255)
                                                                 ccggcTGGGTGGCCCGGCGAGCGGGCCGCGGCGTGGTGACCGAGCTGTAAGGCGGCGCCGCTCCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGGTCCGCCAGCTCAATGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa                                                              <  2:215006/139‑1 (MQ=255)
                                                                    tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                           <  1:704192/143‑1 (MQ=255)
                                                                        ggTGGCGTTGCGTGCGTGGCACGGCGTGTTAACCGAGCTGGTAGGCAGCGCTGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa                                                       <  1:37678/143‑1 (MQ=255)
                                                                        ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGCAGAAATGCTCTa                                                       >  2:739566/1‑143 (MQ=255)
                                                                          tGGCCGTGGGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                     <  1:690461/143‑1 (MQ=255)
                                                                            ggctggCGATCGTGCGACGGGGGGTGAACCGATCTGCGACGTAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                   <  1:1086114/138‑1 (MQ=255)
                                                                            gccgggCGATCGTGCCGGGGCGTGTTAACCGAGGTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATGGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                   <  2:440814/138‑1 (MQ=255)
                                                                             ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc                                                  >  1:725261/1‑143 (MQ=255)
                                                                               ttGCGATCGTGCCACGGCGTGTTAACCGCACGGTTTGGCATCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                >  2:410820/1‑143 (MQ=255)
                                                                               ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAACGCTCTACCAGctc                                                >  1:197305/1‑143 (MQ=255)
                                                                               ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTATTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAGGGCCTACCAGcgc                                                >  2:777927/1‑141 (MQ=255)
                                                                                 tcgacagTGCCACGGCGTGATAACCGAACTGGAATGCAGCGCCGAACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                              <  1:1033669/137‑1 (MQ=255)
                                                                                 gCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CTCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                              <  2:572284/143‑1 (MQ=255)
                                                                                 gCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAGTAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                              >  2:70643/1‑143 (MQ=255)
                                                                                   gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAGTGCTCTAGCAGCTCTTCg                                            >  2:117030/1‑143 (MQ=255)
                                                                                   gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGAACCGCCATG‑AAGG‑CGCAGGATCAGGTTATGCTCCGCCAGCTCATTGAGGGCGCGACGAATAGTCTGCGGGCAGACGGAGAAATGCGCTACCAGctcagac                                            >  2:666444/1‑139 (MQ=255)
                                                                                   gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGACGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGCAATGCTCTACCAGCTCTTCg                                            >  1:1094211/1‑143 (MQ=255)
                                                                                   gATCGTGCCACGGCGTGGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                            <  2:460282/143‑1 (MQ=255)
                                                                                    aTCGTGCCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                           <  2:1105999/143‑1 (MQ=255)
                                                                                          ccccGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCGCCGCCATG‑ATGG‑CGCAGGATAAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                     <  2:266466/140‑1 (MQ=255)
                                                                                               gggtgtCAACCGAGCTGGGAGGCGGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa                                <  2:157121/141‑1 (MQ=255)
                                                                                                 gtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                              <  2:1067740/143‑1 (MQ=255)
                                                                                                   gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcacg                            >  1:71612/1‑140 (MQ=255)
                                                                                                   gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                            <  1:1107666/143‑1 (MQ=255)
                                                                                                   gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                            <  2:657896/143‑1 (MQ=255)
                                                                                                    tcaaCCGACCTGGAAGGCTGCGCCGCTCCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                           <  2:349008/141‑1 (MQ=255)
                                                                                                     tggccGAACTGGAAGGCAGCGCCGCGCCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTCCCAGCTCTTCGGTACTGACATAACCctgc                          <  2:702182/140‑1 (MQ=255)
                                                                                                      aaCCGAACTGGAAGGCGGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                         <  1:452559/143‑1 (MQ=255)
                                                                                                        ccGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAGTGCTCTGCCAGCTGTCCGGTACTGACATAACCctgctgc                       >  1:942210/1‑142 (MQ=255)
                                                                                                        ccGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                       <  2:960572/143‑1 (MQ=255)
                                                                                                         cGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGGGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                      <  1:534546/143‑1 (MQ=255)
                                                                                                         cGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtg                      >  2:264520/1‑142 (MQ=255)
                                                                                                              tGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGTTGTCGCCGCGAATAGTCTGCGGGCTGACGGAGAAATGCTGTACCAGCTCTTCGGTACGTACATCACCCTGCTTTTTCAcc                 >  2:715994/1‑143 (MQ=255)
                                                                                                                   ggCAGCGCCGCACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCCGTGGCCCTTCGGAGAACTGCCCTACGAGCTCTTCGGTACTGACGTAACCCTGCTGTTTAACCAggtg            >  2:863624/1‑140 (MQ=255)
                                                                                                                        cgccgcACCGCCATG‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa       >  2:911386/1‑143 (MQ=255)
                                                                                                                          ccgcACTGCCATG‑ATGG‑CGCAGGTTCAGTTTTTGCTCCGCCAGCTCATTGATGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata     <  2:112625/143‑1 (MQ=255)
                                                                                                                           tcctgcGCCATC‑ATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCCGCAGTTTAACCCGTTCGataatcc    >  2:798944/6‑141 (MQ=255)
                                                                                                                             cACCGCCATG‑ATGG‑CGCAGGATGAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg  <  2:725692/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                               
GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCG  >  NC_000913/3560316‑3560582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A