Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F9 I0 R1 50 62.8 1514203 84.7% 1282529 220.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G91.7% 135.9 / 2.0 48intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base G (35/9);  total (36/12)
Fisher's exact test for biased strand distribution p-value = 4.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑C‑CA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAC  >  NC_000913/3560234‑3560675
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                             
cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATG‑ATGG‑cgacgga                                                                                                                                                                                                                          >  1:687576/1‑222 (MQ=255)
   tGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGGGCCGCACCGCCAAG‑ATGG‑CGCA‑GGacacg                                                                                                                                                                                                                     >  2:35198/1‑225 (MQ=255)
                   aaTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGGTTTGCTCCGCCAGc                                                                                                                                                                                                     >  2:171498/1‑229 (MQ=255)
                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGTCGCGCCGCACCGCCATT‑GTGT‑CGCA‑GGATCAGGTTTTCCTCCGCCAGCt                                                                                                                                                                                                    >  1:169504/1‑228 (MQ=255)
                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCG‑GGACCGGGGTTTGCTCCGCCACCTc                                                                                                                                                                                                   >  2:48094/1‑229 (MQ=255)
                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCAACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGCCACCCCCGCACCGCCACGCGGGG‑C‑CA‑GGATCGGGTTTGGATCGGccgaccc                                                                                                                                                                                                   >  2:576795/1‑224 (MQ=255)
                                 tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTTGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGGGGTcgcg                                                                                                                                                                                       >  1:294946/1‑229 (MQ=255)
                                               gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GAATCAGGTGTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGGCTCCg                                                                                                                                                                         >  1:308254/1‑229 (MQ=255)
                                                cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                        >  1:411124/1‑229 (MQ=255)
                                                    cgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCGGCCCATTCGGGCCCGGGCGAAGAGTcgggtgggtg                                                                                                                                                                    >  2:296388/1‑220 (MQ=255)
                                                        aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCTTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                  >  1:494273/1‑227 (MQ=255)
                                                        aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑CTGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                 >  1:53325/1‑228 (MQ=255)
                                                              tCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCCCATTGCGGTCGCGGCGAAAAGTCTGCGGGCTGACGGGGaaa                                                                                                                                                           >  1:133069/1‑228 (MQ=255)
                                                                                          gCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTTGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGACGAATATTCTGCGGGCTGACGGAGAAATGCTCTACCACCTCTTCGGTACTgcaata                                                                                                                              >  1:612373/1‑224 (MQ=255)
                                                                                           cGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGATTAGCCTGGGGGCTGACGGAGAAATGCCTTACCAACCCTTCGGAACTGACaaaa                                                                                                                             >  2:666942/1‑229 (MQ=255)
                                                                                                gcccATTGGGGGTTTCGCGCCGCCCGTTTGGGGGCGATGCCCTTTTTTTCTGCGGTTGGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                         <  1:48094/227‑1 (MQ=255)
                                                                                                aGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAGGCGAATAGTCTGCGGGCTGACGGAGGAATGCTCTACCAGCCCTTCGGGACCGCCCTAACCctg                                                                                                                        >  1:454562/1‑229 (MQ=255)
                                                                                                     ttGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAGAAGGCTGCGGGCTGACGGAGAAATGCTCTCCAAGCCCTTCGTTACTTCCATAACCCTCCTCtt                                                                                                                   >  2:402232/1‑229 (MQ=255)
                                                                                                             tt‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCCCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAg                                                                                                            >  1:692303/1‑228 (MQ=255)
                                                                                                               ggccccccctgtttGCGGGCGAGTGTCTTTTTTTCGTCGGTCTGGGTGGCCTTGCGTTCGTGCCACGGCGTGTAAACCGAACTGGAGGGCTGCCCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                          <  1:151654/218‑1 (MQ=255)
                                                                                                                   ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTTCGGGCTGGCGGGGAAATGCCCTCCCAGCCTTTCGGTATTACCATAACCCTGCTGTTTAACCAGTTCGat                                                                                                      >  2:14684/1‑229 (MQ=255)
                                                                                                                    cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGatg                                                                                                     >  1:444610/1‑228 (MQ=255)
                                                                                                                     gccttttcgCGGGTGAGGCACTCTTTTGTGTGGGTCTGGGTGTCTTGGCGATCGTGCCTCGGCGTGTTAACCAATCTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata                                                                                                     <  1:666943/220‑1 (MQ=255)
                                                                                                                            tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                             >  1:646258/1‑229 (MQ=255)
                                                                                                                                ggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGCCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGCCCTAACCCTGCTGTTTAACCCGTTCGTTAATACCGTTGTGa                                                                                         >  1:115188/1‑229 (MQ=255)
                                                                                                                                    aTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTCTTGACCTAACCCTGCTGTTTAACCCGTTCGTAAATACCGTGTTGCCGtt                                                                                     >  1:349447/1‑229 (MQ=255)
                                                                                                                                                 cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                         <  1:49442/180‑1 (MQ=255)
                                                                                                                                                 cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                         >  2:49442/1‑180 (MQ=255)
                                                                                                                                                    cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGAAGCGCCCCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGGCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGAACTGATATAACCCTACTGTTTAACCAGTTCGCTACTACCGTTATTACGTTGTGTTTGTTTCTTTta                                                                     >  2:5103/1‑229 (MQ=255)
                                                                                                                                                    cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGCTAATACCGTTTTGACGTTTGGTTTGTTTCCtttt                                                                     >  1:514192/1‑228 (MQ=255)
                                                                                                                                                    cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCACTTCGATAATACCGTTGTGACGTGTGGTTTGTTTCAtttt                                                                     >  2:632939/1‑228 (MQ=255)
                                                                                                                                                    cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCTTTAATACCGTTGTTACGTTGTGTTTGTTTCATTta                                                                     >  1:531430/1‑229 (MQ=255)
                                                                                                                                                           ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGGGCCGTTTTGTTTGTTTCATTTAAAAATcc                                                              >  2:259794/1‑229 (MQ=255)
                                                                                                                                                                 cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGTCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACACAACCCTGCTTGTTAACCAGTTCGAAAATACCGTTGTGACGTGAGTTTTTTTTCTTTTAAAAATCCCTGGaa                                                        >  2:149646/1‑229 (MQ=255)
                                                                                                                                                                          gTGCCACGGCGTGTTAACCGAACTGTAAAGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCCCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCCGCTCTTCGGTCCTGACCACACCCTGCTGCTTAACCAGTTCGTTACTACCGTTTGTACGTTTCATTTTTTTCATTTATAAATCCCGGGAATTAttttct                                               >  2:438082/1‑228 (MQ=255)
                                                                                                                                                                          gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCCGTTTAACCAGTTCGATAATACCGTTGTGGCGTTTTGTTGGTTTCTTTTTTAAATCCCTGGAATTAttttcg                                               >  2:742513/1‑229 (MQ=255)
                                                                                                                                                                          gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTTTGACGTTGTGTTTGTTTCATTTATAATTCCCTGGAATTAttttcg                                               >  1:548752/1‑229 (MQ=255)
                                                                                                                                                                                gggggtgtTAACCGAACTGGAAGGCAGGGCCGCCCCGCCATG‑GTGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtttt                                           <  1:287691/223‑1 (MQ=255)
                                                                                                                                                                                      gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAACTGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCCGTTCGCTAATACCCGTGGTACGTTTCGTTTGTTTCATTTTTAAATCCCCGGAATTATTTTTGTTTTTGCGCAtt                                   >  2:287691/1‑229 (MQ=255)
                                                                                                                                                                                       ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                  >  1:58940/1‑229 (MQ=255)
                                                                                                                                                                                               aCTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTTCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                           <  1:632940/228‑1 (MQ=255)
                                                                                                                                                                                                        ggcgcccgacccgccgTG‑ATGG‑CGCA‑GGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCTGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAACAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc                 <  2:294946/219‑1 (MQ=255)
                                                                                                                                                                                                         ggtgcggctccGCCATG‑ATGG‑CGCA‑GGATCAGGTTTGTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGGCGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                <  1:414392/220‑1 (MQ=255)
                                                                                                                                                                                                         ggcgcCGCCCCGGCAGG‑ATGG‑C‑GAGGGATCAGGTGTGGCTCCGACAGCTGCTTGAGGTAGCGGCGAATAGTCTGGGGGCTGACGGAGAAATGCTCTTCCAGCTCTTCGGTACGGACATAACCCTGCTGTTTAACCAGTTCGATAATCCCGTTGTGACGTTGTGTTTGTTTCAGTTAGAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc                 <  2:129388/227‑1 (MQ=255)
                                                                                                                                                                                                          gccccgcACCGCCATG‑ATGGCC‑CA‑GGATTCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa               <  1:44750/226‑1 (MQ=255)
                                                                                                                                                                                                          cgcccggACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa               <  1:171498/226‑1 (MQ=255)
                                                                                                                                                                                                           cgccgcTCCGCCTTG‑GTGG‑C‑GACGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                <  2:58940/227‑1 (MQ=255)
                                                                                                                                                                                                           cgccgcACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTAGTTTTCGCGCATTTAGCGAATCAACAAAAACCaa              >  2:351697/1‑228 (MQ=255)
                                                                                                                                                                                                                        tg‑atgG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATGGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCCCGCATTGAGCGAATCAACAAAACACATCGCTAAACCCAc  >  1:488369/1‑228 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                             
CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑C‑CA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAC  >  NC_000913/3560234‑3560675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A