Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
715 |
38.4 |
1606192 |
96.6% |
1551581 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,250,629 |
(C)5→6 |
coding (210/1071 nt) |
dhaK ← |
dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,250,624 | 1 | . | C | 96.2%
| 83.7
/ ‑3.2
| 26 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (6/19); total (6/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 |
TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGA > NC_000913/1250486‑1250752
|
tCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAgg < 1:246725/139‑1 (MQ=255)
cGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c > 2:407242/1‑139 (MQ=255)
gTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGAGAGGTGAAAATTTCGCCCGGACAGG‑ccc > 1:729196/1‑139 (MQ=255)
ttCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAg < 2:251218/139‑1 (MQ=255)
aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 2:444290/139‑1 (MQ=255)
aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:26808/139‑1 (MQ=255)
aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:680199/139‑1 (MQ=255)
aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 1:728784/139‑1 (MQ=255)
aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTAGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:611623/139‑1 (MQ=255)
ccGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc > 1:667732/1‑132 (MQ=255)
ccGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:667732/132‑1 (MQ=255)
gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACcaca < 2:260449/139‑1 (MQ=255)
aGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCg < 2:24862/139‑1 (MQ=255)
cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAc < 2:488614/100‑1 (MQ=255)
cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAc > 1:488614/1‑100 (MQ=255)
cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc > 2:192937/1‑139 (MQ=255)
cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc < 1:799981/139‑1 (MQ=255)
tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCTAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa > 2:567229/1‑139 (MQ=255)
tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 1:291161/1‑139 (MQ=255)
gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:155602/56‑1 (MQ=255)
gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc > 1:155602/1‑56 (MQ=255)
cGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGt < 1:376871/139‑1 (MQ=255)
aggtgaAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGACTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACAt < 1:459194/139‑1 (MQ=255)
aaaaTTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 2:78391/139‑1 (MQ=255)
tgttttCGACCGGGCAGGCCCC‑CCGAAAGCATCTCCTGACCGATATAACCACAGTGCATCGGGTCGTGTCCGCGGCCGCCTCACGACAGCAGGGCGACTTTTCCTGCAACATGGACAGCAGCGCGGGTGACATATACCg < 1:716784/136‑1 (MQ=255)
aaaTTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 2:454933/139‑1 (MQ=255)
aaaTTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 1:192937/139‑1 (MQ=255)
aaaTTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAAAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 1:784279/139‑1 (MQ=255)
ttCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATc < 2:395894/139‑1 (MQ=255)
ttCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATc < 2:700435/139‑1 (MQ=255)
cctggACAGG‑CCCGCCGAAAGCAACCCCTGAAAGATACAACCACAGGGCATCGGCTCGTCTCCGCTGCCGCCACCCGACAGAAGAGCGACTTTTCCTGCAACAGGGGCAACAGCTCGGGTGACAGAAAACGGATCCGGa < 1:567229/136‑1 (MQ=255)
|
TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGA > NC_000913/1250486‑1250752
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
