Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,250,629 |
(C)5→6 |
coding (210/1071 nt) |
dhaK ← |
dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,250,624 | 1 | . | C | 100.0%
| 58.1
/ NA
| 17 | A72G (GCC→GGC) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (6/11); total (6/11) |
TTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGCA > NC_000913/1250497‑1250756
|
tttaaaGTTAAGAGTATCGCCGGCGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATCCAGACATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAg < 2:553615/136‑1 (MQ=255)
tAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCt < 2:264663/139‑1 (MQ=255)
aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACGTTCGCCGCCATCAACTTGCATGGCGCATTCAATGATTTTATCGGGCGTCGGTGAGGTGAAAACTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGa < 2:289974/139‑1 (MQ=255)
aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGa < 2:825430/139‑1 (MQ=255)
cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc > 2:157480/1‑139 (MQ=255)
tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGa < 1:526004/97‑1 (MQ=255)
tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGa > 2:526004/1‑97 (MQ=255)
ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAcc < 1:91065/96‑1 (MQ=255)
ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAcc > 2:91065/1‑96 (MQ=255)
tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGa < 1:772152/60‑1 (MQ=255)
tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGa > 2:772152/1‑60 (MQ=255)
cGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAg > 1:717758/1‑43 (MQ=255)
cGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAg < 2:717758/43‑1 (MQ=255)
tCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCggg < 1:157480/139‑1 (MQ=255)
ggtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAcc < 1:585502/46‑1 (MQ=255)
ggtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAcc > 2:585502/1‑46 (MQ=255)
gACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGCa < 2:51227/139‑1 (MQ=255)
|
TTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGCA > NC_000913/1250497‑1250756
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
