Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
759 |
65.2 |
3386260 |
90.7% |
3071337 |
101.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,250,629 |
(C)5→6 |
coding (210/1071 nt) |
dhaK ← |
dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,250,624 | 1 | . | C | 97.7%
| 142.0
/ ‑3.8
| 43 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (23/19); total (24/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCC > NC_000913/1250486‑1250749
|
tCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAgg < 2:617653/139‑1 (MQ=255)
gTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc > 2:566575/1‑139 (MQ=255)
cGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc‑cc < 1:772055/139‑1 (MQ=255)
aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 1:566575/139‑1 (MQ=255)
gAATATCGCCGGTGTAA‑TTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 1:303370/139‑1 (MQ=255)
cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 2:133373/1‑132 (MQ=255)
cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 1:133373/132‑1 (MQ=255)
cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGatat > 2:426872/1‑139 (MQ=255)
cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGatat < 1:426872/139‑1 (MQ=255)
cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGatat > 2:448295/1‑139 (MQ=255)
tgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATccc < 2:1220989/122‑1 (MQ=255)
tgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATccc > 1:1220989/1‑122 (MQ=255)
aTTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 2:1232658/122‑1 (MQ=255)
aTTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 1:1232658/1‑122 (MQ=255)
tttGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCa < 1:291513/138‑1 (MQ=255)
tttGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCa > 2:291513/1‑138 (MQ=255)
gTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCACGTGTCCGc > 2:1146733/1‑139 (MQ=255)
tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCgtgt < 1:1592319/134‑1 (MQ=255)
tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCgtgt > 2:1592319/1‑134 (MQ=255)
cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc > 2:144093/1‑139 (MQ=255)
acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTTCGCTgcc > 2:472840/1‑139 (MQ=255)
cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCCTGTCCGCTgccgc > 2:1123453/1‑139 (MQ=255)
ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAccc > 1:13376/1‑139 (MQ=255)
gccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa > 1:1453612/1‑92 (MQ=255)
gccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 2:1453612/92‑1 (MQ=255)
aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAcag > 2:104739/1‑139 (MQ=255)
aCTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAgg < 1:104739/139‑1 (MQ=255)
cTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAggg < 1:448295/139‑1 (MQ=255)
aTGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACt < 2:13376/139‑1 (MQ=255)
gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACtttt > 1:1598902/1‑139 (MQ=255)
gcATTCAAAGATTTTATCTGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTcc > 2:965678/1‑139 (MQ=255)
aTTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTg < 1:1123453/139‑1 (MQ=255)
ttCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc > 2:1302484/1‑73 (MQ=255)
ttCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 1:1302484/73‑1 (MQ=255)
gATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAgg < 2:1598902/139‑1 (MQ=255)
tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACcaca < 2:76004/76‑1 (MQ=255)
tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCACCGAAAGCATCCCCTGACCGATATAACcaca > 1:76004/1‑76 (MQ=255)
tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 2:1600149/1‑139 (MQ=255)
tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 2:163048/1‑139 (MQ=255)
tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 2:745821/1‑139 (MQ=255)
tCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCa > 1:44967/1‑139 (MQ=255)
ggCGTCGGTGAGGTGAAAATCTCGCCCGGACAGG‑c > 1:932806/1‑35 (MQ=255)
ggCGTCGGTGAGGTGAAAATCTCGCCCGGACAGG‑c < 2:932806/35‑1 (MQ=255)
gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt > 1:224968/1‑74 (MQ=255)
gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt < 2:224968/74‑1 (MQ=255)
cGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCg > 1:1114953/1‑139 (MQ=255)
gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTTCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg < 1:472840/139‑1 (MQ=255)
tCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATcc < 1:144093/139‑1 (MQ=255)
|
TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCC > NC_000913/1250486‑1250749
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
