Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,250,629	(C)_5→6	coding (210/1071 nt)	dhaK ←	dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,250,624	1	.	C	98.6%	251.3 / ‑3.8	69	coding (215/1071 nt)	dhaK	dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (33/35); total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG  >  NC_000913/1250488‑1250754

ggTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cc                                                                                                                                   >  2:1500913/1‑139 (MQ=255)

ggTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAAC‑TGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc                                                                                                                                  >  1:1244262/1‑139 (MQ=255)

        tttCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cccc‑ccgaaa                                                                                                                          >  2:1517732/1‑134 (MQ=255)

        tttCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cccc‑ccgaaa                                                                                                                          >  1:1511552/1‑134 (MQ=255)

        tttCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCAACAAC‑TGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAg                                                                                                                          <  2:1244262/139‑1 (MQ=255)

                   aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa                                                                                                                <  2:1207658/139‑1 (MQ=255)

                     aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc                                                                                                              <  2:1511552/139‑1 (MQ=255)

                        atCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGat                                                                                                           <  1:1500913/139‑1 (MQ=255)

                          cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGc                                                                                                                         >  1:498398/1‑123 (MQ=255)

                          cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGc                                                                                                                         <  2:498398/123‑1 (MQ=255)

                          cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cccc‑ccg                                                                                                                             >  2:570330/1‑116 (MQ=255)

                          cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cccc‑ccg                                                                                                                             <  1:570330/118‑3 (MQ=255)

                               gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc                                                                                                              <  1:125961/129‑1 (MQ=255)

                               gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc                                                                                                              >  2:125961/1‑129 (MQ=255)

                                 gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  2:607637/139‑1 (MQ=255)

                                 gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  1:1517732/139‑1 (MQ=255)

                                 gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  2:1618689/139‑1 (MQ=255)

                                          gATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         >  1:328758/1‑139 (MQ=255)

                                            tAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACGGTGCATCGGCt                                                                                       >  1:1128673/1‑139 (MQ=255)

                                               tCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAgg                                                                                                                                      <  2:733419/90‑1 (MQ=255)

                                               tCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAgg                                                                                                                                      >  1:733419/1‑90 (MQ=255)

                                                     gTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGc                                                                              >  2:1340086/1‑139 (MQ=255)

                                                      tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                    <  1:1493519/84‑1 (MQ=255)

                                                      tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                    >  2:1493519/1‑84 (MQ=255)

                                                        cacCTTCGCCGCCATCAACTTGTATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc                                                                                                              >  1:963305/1‑104 (MQ=255)

                                                        cacCTTCGCCGCCATCAACTTGTATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCTCGAAAGCATCCCCTGAcc                                                                                                              <  2:963305/104‑1 (MQ=255)

                                                        cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAc                                                                                                               <  1:1353905/103‑1 (MQ=255)

                                                        cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAc                                                                                                               >  2:1353905/1‑103 (MQ=255)

                                                           cTTCGCTGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  2:325274/139‑1 (MQ=255)

                                                           cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  2:579787/139‑1 (MQ=255)

                                                           cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  2:1691470/1‑139 (MQ=255)

                                                             tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  >  1:1707559/1‑111 (MQ=255)

                                                             tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  2:1707559/111‑1 (MQ=255)

                                                              cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc                                                                                                              <  1:1538345/98‑1 (MQ=255)

                                                              cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGAcc                                                                                                              >  2:1538345/1‑98 (MQ=255)

                                                              cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAc                                                                     >  1:1689960/1‑139 (MQ=255)

                                                                ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATaa                                                                                                       <  1:406393/103‑1 (MQ=255)

                                                                ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATaa                                                                                                       >  1:427981/1‑103 (MQ=255)

                                                                ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATaa                                                                                                       >  2:406393/1‑103 (MQ=255)

                                                                ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATaa                                                                                                       <  2:427981/103‑1 (MQ=255)

                                                                ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAccc                                                                   >  1:1292704/1‑139 (MQ=255)

                                                                        aaCTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAcagcag                                                           <  2:1292704/139‑1 (MQ=255)

                                                                              cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa                                                                                                                <  1:926857/80‑1 (MQ=255)

                                                                              cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa                                                                                                                >  2:926857/1‑80 (MQ=255)

                                                                                tGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACtt                                                   >  2:1365886/1‑139 (MQ=255)

                                                                                tGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACtt                                                   >  2:29885/1‑139 (MQ=255)

                                                                                  gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa                                                                                                                <  1:35905/76‑1 (MQ=255)

                                                                                  gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa                                                                                                                >  2:35905/1‑76 (MQ=255)

                                                                                   cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTc                                                >  2:940488/1‑139 (MQ=255)

                                                                                     cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGc                                                                                                                         >  2:48982/1‑64 (MQ=255)

                                                                                     cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGc                                                                                                                         <  1:48982/64‑1 (MQ=255)

                                                                                     cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACttt                                                  <  1:1689252/135‑1 (MQ=255)

                                                                                     cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACttt                                                  >  2:1689252/1‑135 (MQ=255)

                                                                                          aaaGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAAc                                         <  2:1128673/139‑1 (MQ=255)

                                                                                            aGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa                                                                                                                <  1:1269387/66‑1 (MQ=255)

                                                                                            aGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGa                                                                                                                >  2:1269387/1‑66 (MQ=255)

                                                                                                  tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt                                 >  2:825751/1‑139 (MQ=255)

                                                                                                  tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt                                 >  1:647969/1‑139 (MQ=255)

                                                                                                        gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt                                 >  1:793338/1‑133 (MQ=255)

                                                                                                        gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt                                 <  2:793338/133‑1 (MQ=255)

                                                                                                        gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc                           >  2:902893/1‑139 (MQ=255)

                                                                                                          gacGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg                         >  2:1040848/3‑139 (MQ=255)

                                                                                                          gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg                         <  1:1691470/139‑1 (MQ=255)

                                                                                                           tCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg                         >  2:925843/1‑138 (MQ=255)

                                                                                                           tCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg                         <  1:925843/138‑1 (MQ=255)

                                                                                                             ggtgaggtgaAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTg                      <  1:940488/139‑1 (MQ=255)

                                                                                                              gtgaggtgaAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg                         <  1:1351426/135‑1 (MQ=255)

                                                                                                              gtgaggtgaAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg                         >  2:1351426/1‑135 (MQ=255)

                                                                                                                gaggtgaAAATTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACa                   <  1:29885/139‑1 (MQ=255)

                                                                                                                      aaaaTTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGc                                   <  1:882390/117‑1 (MQ=255)

                                                                                                                      aaaaTTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGc                                   >  2:882390/1‑117 (MQ=255)

                                                                                                                      aaaaTTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAg                              >  1:59142/1‑122 (MQ=255)

                                                                                                                      aaaaTTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAg                              <  2:59142/122‑1 (MQ=255)

                                                                                                                      aaaaTTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc                             <  1:96330/123‑1 (MQ=255)

                                                                                                                      aaaaTTTCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc                             >  2:96330/1‑123 (MQ=255)

                                                                                                                           ttCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAca                                                               <  1:1033861/84‑1 (MQ=255)

                                                                                                                           ttCGCCCGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATc        <  1:825751/139‑1 (MQ=255)

                                                                                                                           ttCGCCCGGACAGGCCCCC‑CGAAAGCAGCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAca                                                               >  2:1033861/1‑84 (MQ=255)

                                                                                                                                 cGGACAGGCCCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATg  <  2:1689960/139‑1 (MQ=255)

GGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCC‑CGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG  >  NC_000913/1250488‑1250754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A