Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,250,629 |
(C)5→6 |
coding (210/1071 nt) |
dhaK ← |
dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,250,624 | 1 | . | C | 93.5%
| 102.8
/ 0.4
| 31 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (14/15); total (15/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 |
CGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGCAGT > NC_000913/1250487‑1250758
|
cGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c > 2:675009/1‑139 (MQ=255)
cGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c < 1:446694/139‑1 (MQ=255)
gTCGCTGTTTCAAAGTTAAGTATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc > 2:546708/1‑139 (MQ=255)
tCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑ccc‑c > 2:594817/1‑139 (MQ=255)
ttCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAg < 2:245651/139‑1 (MQ=255)
tCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGc < 2:170261/139‑1 (MQ=255)
tCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGc < 2:411382/139‑1 (MQ=255)
aaGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTg > 2:335563/1‑139 (MQ=255)
aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGAcc < 1:712111/139‑1 (MQ=255)
tatCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGa < 1:722043/139‑1 (MQ=255)
cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGatat > 2:577675/1‑139 (MQ=255)
aGTACACCTTCGCCGCCATCATCTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCg < 1:546708/139‑1 (MQ=255)
tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGAcc < 2:591972/106‑1 (MQ=255)
tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGAcc > 1:591972/1‑106 (MQ=255)
tACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCt > 1:579326/1‑139 (MQ=255)
cacGTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCa > 2:599213/1‑94 (MQ=255)
cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCa < 1:599213/94‑1 (MQ=255)
cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc < 1:675009/139‑1 (MQ=255)
cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCACC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc > 2:332592/1‑139 (MQ=255)
acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGatat > 1:111336/1‑108 (MQ=255)
acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGatat < 2:111336/108‑1 (MQ=255)
acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgcc > 1:100522/1‑139 (MQ=255)
tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTGTCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTTGTGTCCGCTGCCGCCa > 1:462019/1‑139 (MQ=255)
tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa > 1:809293/1‑139 (MQ=255)
tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa > 2:486064/1‑139 (MQ=255)
cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAc > 2:222439/1‑139 (MQ=255)
cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAc > 1:522286/1‑139 (MQ=255)
cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGAcc < 2:284939/75‑1 (MQ=255)
cATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGAcc > 1:284939/1‑75 (MQ=255)
aaGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACa < 2:522286/139‑1 (MQ=255)
tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 1:63346/1‑139 (MQ=255)
gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTc < 2:579326/139‑1 (MQ=255)
gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg < 2:100522/139‑1 (MQ=255)
gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg < 1:335563/139‑1 (MQ=255)
cAGG‑CCC‑CCTGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGCAgt < 1:594817/139‑1 (MQ=255)
|
CGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CC‑GAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATGCAGT > NC_000913/1250487‑1250758
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
