Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I1 R1 759 71.0 3673240 91.5% 3361014 104.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,250,629 (C)5→6 coding (210/1071 nt) dhaK ← dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,250,6241.C100.0% 203.6 / NA 59A72G (GCC→GGC) dhaKdihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (28/31);  total (28/31)

TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG  >  NC_000913/1250486‑1250754
                                                                                                                                           |                                                                                                                                  
tCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAgg                                                                                                                                     <  2:402017/139‑1 (MQ=255)
  ggTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cc                                                                                                                                  >  1:1203696/1‑139 (MQ=255)
      gCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑cccccc                                                                                                                              >  1:1002380/1‑138 (MQ=255)
            tCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGc                                                                                                                         <  1:77941/139‑1 (MQ=255)
                      gAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAc                                                                                                               <  1:482479/139‑1 (MQ=255)
                      gAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAc                                                                                                               <  2:1638654/139‑1 (MQ=255)
                       aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAcc                                                                                                              <  2:447204/139‑1 (MQ=255)
                            cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGatat                                                                                                         <  1:574189/139‑1 (MQ=255)
                            cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                   <  1:1557249/112‑1 (MQ=255)
                            cGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                   >  2:1557249/1‑112 (MQ=255)
                              ccGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATaa                                                                                                       >  1:361708/1‑139 (MQ=255)
                                 gtgtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACca                                                                                                    <  2:45075/139‑1 (MQ=255)
                                   gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  1:1188442/139‑1 (MQ=255)
                                   gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  2:361708/139‑1 (MQ=255)
                                                 tCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         >  1:980909/1‑134 (MQ=255)
                                                 tCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         <  2:980909/134‑1 (MQ=255)
                                                          cacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgc                                                                           <  2:922543/139‑1 (MQ=255)
                                                           acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCAT‑‑CCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  1:890570/1‑139 (MQ=255)
                                                             cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  2:1522675/1‑139 (MQ=255)
                                                             cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  1:1522675/139‑1 (MQ=255)
                                                             cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTG‑AAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgcc                                                                       >  2:764346/1‑139 (MQ=255)
                                                               tCGCCGCCATCAACTTGCATTGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa                                                                      >  1:1080269/1‑139 (MQ=255)
                                                                cgccgcCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGCTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAc                                                                     >  1:1318586/1‑139 (MQ=255)
                                                                  ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGGAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAc                                                                                                               >  2:667671/1‑95 (MQ=255)
                                                                  ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGGAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGAc                                                                                                               <  1:667671/95‑1 (MQ=255)
                                                                  ccgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAccc                                                                   <  2:809516/139‑1 (MQ=255)
                                                                       aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  >  1:1107848/1‑103 (MQ=255)
                                                                       aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACcaca                                                                                                  <  2:1107848/103‑1 (MQ=255)
                                                                       aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         >  1:1640738/1‑112 (MQ=255)
                                                                       aTCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCgg                                                                                         <  2:1640738/112‑1 (MQ=255)
                                                                                cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                   <  1:615847/60‑1 (MQ=255)
                                                                                cATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c                                                                                                                                   >  2:615847/1‑60 (MQ=255)
                                                                                    gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACtttt                                                 >  2:450722/1‑139 (MQ=255)
                                                                                     cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  2:1025649/115‑1 (MQ=255)
                                                                                     cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  1:1025649/1‑115 (MQ=255)
                                                                                     cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTc                                                >  1:1021419/1‑139 (MQ=255)
                                                                                     cgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTc                                                >  2:26137/1‑139 (MQ=255)
                                                                                          tCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCa                                           >  1:1273668/1‑139 (MQ=255)
                                                                                            aaaGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAAc                                         <  1:1676123/139‑1 (MQ=255)
                                                                                                  tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGc                                   <  1:450722/139‑1 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATCTAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt                                 >  1:866802/1‑139 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACca                                                                                                    <  2:1019180/72‑1 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACca                                                                                                    >  1:1019180/1‑72 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGGATCGGCTCGTGTCCGCTGCCGCCCCCCGACAGCATGGCGACTTTTCCTGCAACAGGGGCAt                                 >  1:1168211/1‑139 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  2:103778/100‑1 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  1:103778/1‑100 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        <  1:317342/100‑1 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc                                                                        >  2:317342/1‑100 (MQ=255)
                                                                                                    tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt                                 >  1:238614/1‑139 (MQ=255)
                                                                                                        gggCGTCGGTGAGGTGAAAATATCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGc                             <  2:1080269/139‑1 (MQ=255)
                                                                                                            gTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCgg                         <  2:238614/139‑1 (MQ=255)
                                                                                                             tCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCggg                        >  2:1547633/1‑139 (MQ=255)
                                                                                                               ggtgaggtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTg                      <  1:26137/139‑1 (MQ=255)
                                                                                                                    ggtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt                                                                                            >  2:279666/1‑64 (MQ=255)
                                                                                                                    ggtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt                                                                                            <  1:279666/64‑1 (MQ=255)
                                                                                                                     gtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCa                                                                                                                        >  2:327571/1‑35 (MQ=255)
                                                                                                                     gtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCa                                                                                                                        <  1:327571/35‑1 (MQ=255)
                                                                                                                     gtgaAAATTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATac                >  1:1146217/1‑139 (MQ=255)
                                                                                                                         aaaTTTCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCACGGCGACTTTTTCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg            <  2:1168211/139‑1 (MQ=255)
                                                                                                                              tCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt                                                                                            <  2:383449/54‑1 (MQ=255)
                                                                                                                              tCGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCAt                                                                                            >  1:383449/1‑54 (MQ=255)
                                                                                                                               cGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGatat                                                                                                         >  2:742448/1‑40 (MQ=255)
                                                                                                                               cGCCCGGACAGGCCCCCCGAAAGCATCCCCTGACCGatat                                                                                                         <  1:742448/40‑1 (MQ=255)
                                                                                                                                   cGGACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTACCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATg  <  2:1318586/139‑1 (MQ=255)
                                                                                                                                    ggACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACa                                        <  2:1385969/100‑1 (MQ=255)
                                                                                                                                    ggACAGGCCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACa                                        >  1:1385969/1‑100 (MQ=255)
                                                                                                                                           |                                                                                                                                  
TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGATG  >  NC_000913/1250486‑1250754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A