Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F50 I0 R1	80	46.5	1794022	91.0%	1632560	142.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	800,807	G→T	100%	A17S (GCG→TCG)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	800,807	0	G	T	100.0%	97.3 / NA	35	A17S (GCG→TCG)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (15/20); total (15/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800667‑800940

ttattaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGctcg                                                                                                                                     >  1:3245/1‑143 (MQ=255)

   ttaACCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTc                                                                                                                                  <  1:485680/143‑1 (MQ=255)

         gTTGAGCTACAGGCATTAGAAATGCTTTGCCCTTAAAGTAATCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTAAGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTa                                                                                                                            <  2:753121/143‑1 (MQ=255)

          ttGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACAATATCGCAGTAAGTTATGTTTTTAAATTGAGGGCATTATTATGACAAAAATACCCTACCTGCTGATGCGAGGTGGAACCTCGAGGGGCTCGCTCCTGTAAg                                                                                                                           >  2:324584/1‑143 (MQ=255)

                     tCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATGTGCTACGTGATGATGCGAGGTGGAACCTCGAGGGGATCGTTCCTGTTAGCGGAACATTTa                                                                                                                >  2:535066/1‑143 (MQ=255)

                        ttAGAGATGCTTTGCCCTTAATGTAACCATATCGCCATAAGTTATGTTTTTAAATTGAGGGCATTAATATGAAAAACATACCCTGCGAGATGATGCGAGGTGGAACCTCGGGGGGCTCGTTCCTGTAAGCGGAACATTTAccc                                                                                                             >  2:121969/1‑143 (MQ=255)

                        ttAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGATCCTGTTACCGGACCACATAccc                                                                                                             >  2:610519/1‑143 (MQ=255)

                            agaAGCTTTGGACTAAATGTAACCATAGCGCAATAAGATATGTGTTTAAATTGAGGGCATTATAAAGAAGAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAg                                                                                                         <  2:741448/143‑1 (MQ=255)

                              aTGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTGGCGGAACATTTCCCCGAAGAt                                                                                                       >  1:242106/1‑143 (MQ=255)

                                 cTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACTCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTACAGGTAGCGGAAGATATACCCGAAGGTcca                                                                                                    >  2:340359/1‑141 (MQ=255)

                                  ttttccattAATGTACTCTTATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCaaa                                                                                                   <  2:756650/136‑1 (MQ=255)

                                  ttcgCCCTTGACGTAACCATATCGCAATAAGTTATGTTCTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGCTGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCaaa                                                                                                   <  1:119028/140‑1 (MQ=255)

                                   ttGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGCAGATCAAAc                                                                                                  >  1:41879/1‑143 (MQ=255)

                                        cTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTCCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgc                                                                                             <  2:542395/143‑1 (MQ=255)

                                         ttAATGTAACCAAACGTGAATCAGTTATGTTTTTAAATTGAGGGGATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgcg                                                                                            <  2:529593/143‑1 (MQ=255)

                                                        tgCAATTAGTTATGTTTTTAAGTTGAGGGTGTTATTATTAAAAATATACCCTGCGTGGTGATGCGAGGTGGAACGTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt                                                                             <  1:239390/142‑1 (MQ=255)

                                                        cGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTAGGGGGGCTCCTCCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt                                                                             <  1:847721/143‑1 (MQ=255)

                                                            aTAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCa                                                                         >  1:140504/1‑143 (MQ=255)

                                                                   aTGTTTGTGAAGTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGGGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATgg                                                                  <  1:587636/143‑1 (MQ=255)

                                                                         gtAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCg                                                            <  1:738761/142‑1 (MQ=255)

                                                                             aTTGAGGGAATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACATCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTaa                                                        <  1:801272/143‑1 (MQ=255)

                                                                                gAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGa                                                     <  1:818378/143‑1 (MQ=255)

                                                                                gAGGGCATTAGTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGa                                                     <  1:818035/143‑1 (MQ=255)

                                                                                       ttgttatGAAAAAAATACCCTGCGTGATGAGGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa                                              <  1:364826/140‑1 (MQ=255)

                                                                                       ttattaTGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa                                              <  1:64250/143‑1 (MQ=255)

                                                                                                         ccTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAGGATCAAACGCAGCGCGATAAACTCTTGATGGCACATTTGGGTGAAGGGAAACACCAGGAACTTGACGGTTTTggcggc                            >  2:583364/1‑143 (MQ=255)

                                                                                                         ccTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGCTATTggcggc                            >  2:376930/1‑143 (MQ=255)

                                                                                                           tGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGcggcgg                          <  2:610283/143‑1 (MQ=255)

                                                                                                           tGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGcggcgg                          <  2:401062/143‑1 (MQ=255)

                                                                                                            gCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCAGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGAGTTCAGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGt                         >  2:175193/1‑143 (MQ=255)

                                                                                                               tgatgaTGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATCTGGTGCTGTTGCCAACTATGGGTTCCGGTCACGATCTGGAAATTGCCGGTATTGGCGGCGGTAAt                      >  1:203913/1‑143 (MQ=255)

                                                                                                                gatgatGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATc                     <  1:878507/143‑1 (MQ=255)

                                                                                                                    atgCGAGGTGGAACCTCGGGGGGCTCGTTCCTGTTCGCGGAACAGTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt                 <  1:98257/143‑1 (MQ=255)

                                                                                                                           gTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGt          >  2:369422/1‑143 (MQ=255)

                                                                                                                                 ccTCGAGGGGCTCGTTCCTGTTAGCGGAACATATACACGAAGAGCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAGAGTc    >  2:191859/1‑143 (MQ=255)

                                                                                                                                   tCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGCTCTGGCAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGc  >  1:439364/1‑143 (MQ=255)

TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800667‑800940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A