Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F91 I0 R1
|
49 |
33.8 |
1296440 |
89.8% |
1164203 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
800,807 |
G→T |
100% |
A17S (GCG→TCG) |
ybhH → |
putative isomerase YbhH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 800,807 | 0 | G | T | 100.0%
| 74.8
/ NA
| 27 | A17S (GCG→TCG) | ybhH | putative isomerase YbhH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (10/17); total (10/17) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC > NC_000913/800670‑800940
|
ttaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAAAG‑ATGCATGATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTc < 1:612351/143‑1 (MQ=255)
taATCGTGAAGGTAAAGGCATGAGAGATTCGTTGGCGTTAATGTATACAT‑ATCGCAATAAGTGATGTGTTTAAAGTGAGGGCATTATGATGAAAAAAATACCCTGCGTGAGGATGCGAGGTGGAACCTCGAGGGGCTCGTTcc < 2:468104/143‑1 (MQ=255)
ttGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAAGCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTAGGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAAGCTCGAGGGGCTCGTCCCTGTTAg > 2:316519/1‑143 (MQ=255)
tGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTCTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGc < 1:325949/143‑1 (MQ=255)
gCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGCGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGa > 1:373394/1‑143 (MQ=255)
tCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTCGCGGACCAtttc > 2:75669/1‑142 (MQ=255)
ttAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTAccc > 2:604625/1‑143 (MQ=255)
gagaTTCTTTGACCTTAATGTAACCAT‑AGCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGaa < 2:394860/143‑1 (MQ=255)
aTGTAACCGT‑ATCGCAATAAGCTATGTTTTTAAATTGAGGGCAATAATAAAAAATAAAGACCCGGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGa < 2:559624/143‑1 (MQ=255)
gTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAGGATTAAACGCCGTGCGATa > 1:292873/1‑143 (MQ=255)
cGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt < 1:113079/143‑1 (MQ=255)
cAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGACAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAGGATCAGACGCAGCGCGATAAAGTATTGATgg > 1:222245/1‑143 (MQ=255)
aaTTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAAAGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTa > 1:436778/1‑143 (MQ=255)
ttGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAAc < 2:557862/143‑1 (MQ=255)
gggCATGATTATGGAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATc < 2:23488/143‑1 (MQ=255)
ttattaTGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa < 1:322076/143‑1 (MQ=255)
atGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGTGCTCGTTCCTGTTAACGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGa < 1:2369/143‑1 (MQ=255)
aaaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTa > 1:566466/1‑143 (MQ=255)
cTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATAGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGgcggcg < 2:628107/143‑1 (MQ=255)
cTGCGAAATGATGCGAGGTGGAAACCCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGgcggcg < 1:396554/143‑1 (MQ=255)
tgaggCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCa < 2:212408/143‑1 (MQ=255)
gatgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCAc > 1:319377/1‑143 (MQ=255)
gtggggggTGGAACCGCGGGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt < 2:310983/137‑1 (MQ=255)
tgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTg > 1:129239/1‑143 (MQ=255)
cGATGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGAc < 1:237900/143‑1 (MQ=255)
gAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTaaa < 1:23391/143‑1 (MQ=255)
gcGAGGCGCTCGTGCCTGTTAGCGGAACATTTACACGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGc < 2:236980/142‑1 (MQ=255)
|
TTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC > NC_000913/800670‑800940
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
