Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F21 I1 R1	22	41.7	1602136	90.4%	1448330	141.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	800,807	G→T	A17S (GCG→TCG)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	800,807	0	G	T	100.0%	126.3 / NA	41	A17S (GCG→TCG)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/22); total (19/22)

TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800667‑800940

ttattaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGctcg                                                                                                                                     >  2:27359/1‑143 (MQ=255)

       tCGTTGATCTAAAGTCATTAGAGATGCTTTGCCCTTAAGTTAACCATATAGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGt                                                                                                                              <  1:610275/143‑1 (MQ=255)

             aGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCgg                                                                                                                        <  1:621914/143‑1 (MQ=255)

             aGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCgg                                                                                                                        <  1:621829/143‑1 (MQ=255)

             aGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCgg                                                                                                                        >  1:755438/1‑143 (MQ=255)

              gCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGa                                                                                                                       >  1:59903/1‑143 (MQ=255)

              gCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGAGGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGa                                                                                                                       >  1:278537/1‑143 (MQ=255)

                tAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTAATATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGCTAGCGGCAc                                                                                                                     >  1:544570/1‑143 (MQ=255)

                 aaaGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACa                                                                                                                    >  1:710686/1‑143 (MQ=255)

                     tCATTAGAGCTGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTa                                                                                                                <  2:460685/143‑1 (MQ=255)

                                  cttGCCCGTAATGGAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCaaa                                                                                                   <  1:379434/142‑1 (MQ=255)

                                      ccattAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCa                                                                                               <  1:252709/140‑1 (MQ=255)

                                        cTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgc                                                                                             >  1:4942/1‑143 (MQ=255)

                                         ttAATGTAACCATATCGTAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGCAGATCAAACGCAgcg                                                                                            <  2:673406/143‑1 (MQ=255)

                                            aTGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATTAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGa                                                                                         >  1:339605/1‑143 (MQ=255)

                                                        ggaaataatTTATGTGTTGAAATTGATTGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt                                                                             <  2:669787/140‑1 (MQ=255)

                                                          cAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATgg                                                                           <  1:775449/143‑1 (MQ=255)

                                                             tAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCaa                                                                        >  2:520718/1‑143 (MQ=255)

                                                                 ttATGTTTTTAATTTGAGGGCATTATTATGAGAAAAATACCGTGCGTGATGATGCGAGGTGGAACCGCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTAt                                                                    <  1:389142/143‑1 (MQ=255)

                                                                 ttATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTAt                                                                    >  1:531303/1‑143 (MQ=255)

                                                                   aTGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATgg                                                                  <  2:755438/143‑1 (MQ=255)

                                                                    tGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTCCTCGATGATGCGAGGTGAAACCTCGAGAGGCTCGATCCTATTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAAATATggg                                                                 >  1:800669/1‑143 (MQ=255)

                                                                       tgttACATTGGGGGCATTATTATGGAAACAATACCCTCAGTGACAATGCGAGGTGGAACCTCGAGGGGCTCGGTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGCTGGCAATTATGGGTTc                                                              <  2:574989/141‑1 (MQ=255)

                                                                        tttAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGATTcc                                                             >  2:695868/1‑143 (MQ=255)

                                                                        tttAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTAACCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTcc                                                             >  1:730803/1‑143 (MQ=255)

                                                                             aTTGAGGGCATTATTATGAAAAAAATACCCGGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTaa                                                        <  1:360452/143‑1 (MQ=255)

                                                                                  gggCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATc                                                   <  1:531927/143‑1 (MQ=255)

                                                                                       ttattaTGAAAAAAAGACCCTGCGTGATGGTGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa                                              <  2:618906/143‑1 (MQ=255)

                                                                                         attatGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGAGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCGATTATGGGTTCCGGTAACGATCTGGAAAt                                            >  1:110395/1‑143 (MQ=255)

                                                                                          tgatGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAAtt                                           <  2:751018/141‑1 (MQ=255)

                                                                                            atGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGTCAATTATGGGTTCCGGTAACGATCTGGAAATTGa                                         >  1:81686/1‑143 (MQ=255)

                                                                                            atGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGa                                         >  1:525766/1‑143 (MQ=255)

                                                                                             tGAAAAAAATACCCCGCATGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGAc                                        <  1:45589/143‑1 (MQ=255)

                                                                                                aaaaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGGTCTGGAAATTGACGGt                                     >  1:90576/1‑143 (MQ=255)

                                                                                                  aaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTAt                                   <  1:763075/143‑1 (MQ=255)

                                                                                                              gTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGGTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTaa                       <  1:27359/143‑1 (MQ=255)

                                                                                                                  tgattCGAGGTGGATCCTCGTGTGGCTCATTCCTGTTAGCGGAACCTTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCa                   <  1:131935/143‑1 (MQ=255)

                                                                                                                    gtgCGAGGAGGAACCTCGAGGGGCTCCCTCCTAGTGGCTGAATATCTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGTCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt                 <  1:752910/142‑1 (MQ=255)

                                                                                                                        gAGGTGGAACCTCGAGGGGCTCGGTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGAAAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTAGAAATTGACGGTATTGGCGGCGGAACTCCCCTGAcc             >  2:727651/1‑143 (MQ=255)

                                                                                                                         aGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCa            >  1:676578/1‑143 (MQ=255)

                                                                                                                              gAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGCAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTaag       >  1:125993/1‑142 (MQ=255)

                                                                                                                                   tCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAGAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGc  <  2:798/143‑1 (MQ=255)

TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800667‑800940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A