Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	18	114.9	2145065	94.8%	2033521	261.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	96.3%	292.0 / 0.8	84	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (35/44); total (37/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCG  >  NC_000913/3560183‑3560710

cACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑cg                                                                                                                                                                                                                                                                  <  1:764397/274‑2 (MQ=255)

               tgCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTGATGG‑CGCAGG‑ATCAGGTTTTGc                                                                                                                                                                                                                                                 >  1:431332/1‑275 (MQ=255)

                    aaaTCTTCTTTTCCAATGAACGTGTTAGCAACGTTGAGATTGTTGGTGAAAATGCGCAAATTGCTGTGATTGGGCAGTGCGGGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCg                                                                                                                                                                                                                                             <  2:533487/274‑1 (MQ=255)

                    aaaTCTTCTTTTACCCTCAACGGGGTGGCAACGTTGAGAGTGTTGGTGACAATGCGAAAATTGCTGGGATTGAGCAGTGCTTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCg                                                                                                                                                                                                                                             <  2:429345/274‑1 (MQ=255)

                      aTCTTCTTTTACCATCAACGGGTGAGCAACGGTGGGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                                           <  2:545571/274‑1 (MQ=255)

                         ttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAg                                                                                                                                                                                                                                         <  1:792485/273‑1 (MQ=255)

                         ttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                                        >  1:909967/1‑274 (MQ=255)

                         ttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGAGTCGAGCCATTGGGGATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAATGGGAAGGCCGGCACGCACCGCCATACTGGCC‑CAGG‑ACCAGGCTTTGCTCCGCCAGcc                                                                                                                                                                                                                                       >  2:369430/1‑274 (MQ=255)

                         ttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGGGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAg                                                                                                                                                                                                                                         <  1:83558/273‑1 (MQ=255)

                            ttttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCCGCCCAt                                                                                                                                                                                                                                    >  1:184289/1‑275 (MQ=255)

                              ttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAAAAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                                  >  2:85875/1‑275 (MQ=255)

                                       acgggttgacAACGTTGGGATTGTTGGGGAAAATGCGCAAATGGCTGTGGTTGGGCAGTGCGTGCGCTGCCGCGTCCGGCGTGGGGCCGATGTTGATAAACGGCGTCGTGCCATTGGGGATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCGGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                           <  2:583226/264‑1 (MQ=255)

                                              aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAg                                                                                                                                                                                                                  >  1:703499/1‑275 (MQ=255)

                                                       gggtttggtgGGGAAAATGCGAAAATGGCTGTGATTGGGCAGTGCGTGCGCTACGGCTGCCGGCGTGGGGCCGATATCGATAAACAGGGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                                                         <  2:282026/271‑1 (MQ=255)

                                                              ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTTCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTAAGGTCGCGGCCACTAGTCTGCggggcgccggg                                                                                                                                                                                                  >  2:669891/1‑267 (MQ=255)

                                                              ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGga                                                                                                                                                                                                  >  1:344523/1‑275 (MQ=255)

                                                                gggtaCAATGCGCAAATTGCGGTGATTGGGCAGGGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                                                                                                                                                <  2:267697/271‑1 (MQ=255)

                                                                   gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATCGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                                                                                                                                             >  1:401305/1‑275 (MQ=255)

                                                                    aCAATGCGCAAATTGGTGTGATTGGGCAGTGCGTGGGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                                                                                                                                            <  2:909966/275‑1 (MQ=255)

                                                                    aCAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCGTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATg                                                                                                                                                                                            >  1:844853/1‑275 (MQ=255)

                                                                    aCAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                                                                                                                                             <  2:136909/274‑1 (MQ=255)

                                                                        tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGCTATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGG‑CGCCGG‑GTCGGGTTTTGCTCCGCCCGATCCTTGCGGGCCCGGCGAATCGTCCGCGTCACGACGGAGACAttcact                                                                                                                                                                                        >  2:783382/1‑270 (MQ=255)

                                                                        tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGc                                                                                                                                                                                                                                                 >  2:1066072/1‑218 (MQ=255)

                                                                        tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctct                                                                                                                                                                                        >  1:860484/1‑275 (MQ=255)

                                                                        tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCCCATTGAGGGCGGGGCGAATAGTCTGCGGGCTGCCGGAGGAATGctc                                                                                                                                                                                         >  2:494006/1‑274 (MQ=255)

                                                                        tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCCCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCCCCCGCCCATTGAGGTCGCGGCGACTAGTCTGCGGGCTGCCGGACAAATGctct                                                                                                                                                                                        >  2:161910/1‑275 (MQ=255)

                                                                            cAAATTGGTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCg                                                                                                                                                                                                                                             <  1:1066073/218‑1 (MQ=255)

                                                                            cAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                                                                                                                                            <  1:494006/267‑1 (MQ=255)

                                                                             aagttGCTGGGATGGAGCAGTGGGTGCCCTACGGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTGTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                   <  2:711708/272‑1 (MQ=255)

                                                                             aaaTTGCTGTGATTGAGCAGTGGGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                   <  2:820482/275‑1 (MQ=255)

                                                                             aaaTTGCTGTGATTGAGCAGTGGGGGCGGTACCGCTTCCGTGGTGGTGCTGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATTCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGT‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                   <  1:791141/275‑1 (MQ=255)

                                                                             aaaTTGCTGTGAGTGAGCAGTGCGTGCGCTACCGCTTCGGGGGTGGTGCGGATATCGAGAAACAGCGTCGACGCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                   <  1:541410/275‑1 (MQ=255)

                                                                                   cTGTGATTGAGCAGTGCGGGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                              <  1:984845/274‑1 (MQ=255)

                                                                                   cTGGGATTGAGCAGTGCGTGCGCGACCGTTCCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTGTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                              <  2:689894/274‑1 (MQ=255)

                                                                                       gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                                         <  2:191748/275‑1 (MQ=255)

                                                                                         ttGGGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                        <  1:138022/274‑1 (MQ=255)

                                                                                                 tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                                                                                                                                               >  2:260729/1‑275 (MQ=255)

                                                                                                   ggtgcgCGACCGCTTCCGGCGGGGTGGCGATTTCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                                                             <  2:1018230/274‑1 (MQ=255)

                                                                                                      gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAAATAACCcggc                                                                                                                                                          >  1:314763/1‑275 (MQ=255)

                                                                                                           aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCCCCGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACAAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                                                     >  1:292707/1‑275 (MQ=255)

                                                                                                                  cgggggtggggGCGATTTCGATAAGCGGCGTGGAGCCCTTGGGGATTTGGTGCGCCACGTTGCGGGAGATGCGCTCGTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCGCGGCGTGTGAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCGTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                                               <  2:966071/269‑1 (MQ=255)

                                                                                                                  ccGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                                               <  2:878657/274‑1 (MQ=255)

                                                                                                                    ggCTTGGTGCCAATATCGATAAACAGCGTCGTGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                                                             <  2:703499/274‑1 (MQ=255)

                                                                                                                         gtgtgcGATATCTATAAACAGCGTCGGGCCGTTGGGGATTTGCTCCTCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACTGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa                                                                                                                                       <  1:369430/270‑1 (MQ=255)

                                                                                                                          gtgtctatatCGTGAGACAGAGGCGAGCCAGTGGGGATTTGCGCCGCCCGGTTGCGGGCGCTGCTCTCGTTTTCTTGGGGCTGGGTGGCCTTGCGGTCGTGGCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCGATGATGG‑CGCAGG‑ATCAGGTTTTGGTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa                                                                                                                                       <  2:813468/268‑1 (MQ=255)

                                                                                                                           tgCCGATATCGATAAAGAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                                                     <  1:115978/275‑1 (MQ=255)

                                                                                                                                     gATAAACAGCGTCGAGCCCTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTCTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCCTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑CTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTTGACCAGCCCTTCGCGACTGACCTAACCCTGCTGTTTAACCCGTTCGAAAATAcctggtctc                                                                                                                            >  2:366443/1‑267 (MQ=255)

                                                                                                                                     gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt                                                                                                                                                                       >  1:518369/1‑231 (MQ=255)

                                                                                                                                       tAAACAGCGTCTAGCCCTTGAGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGCGTGGCCTTCCGATCCTGCCACGGCGTGTTAACCGAACTGGACGGCATCGCCGCAACGCCATGATTG‑CACATC‑ACCAGGATTGTCTCCGCCTGCTCATTGAGCCCCAGGCGAATAGCCTGCGCCCTGACGCAGAAAGCCGCTGCTACGCAATCGTTACTCACCTGACCCTGCTGGTTCACCAAGTCGATAACACCGtttcaacgt                                                                                                                         >  2:401116/1‑268 (MQ=255)

                                                                                                                                       tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                                          <  1:67577/274‑1 (MQ=255)

                                                                                                                                       tAAACAGCGTCGAGCCATTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                                          <  1:249743/274‑1 (MQ=255)

                                                                                                                                         aaCGGCGTCGAGCCATTGGGGATTTGGTGCGCCACTTGGCGGGCGATGCGGTCTTTTTCTTCGGTCTGGGTGGCCTTGCGGTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGGACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                                                                                                                              <  1:366443/252‑1 (MQ=255)

                                                                                                                                         aaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                                                                                                                                                   <  2:518369/231‑1 (MQ=255)

                                                                                                                                               gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCATACCAGCGCTTCGGTACCGCCATAACCCTGCTGTTTAACCAGTTCGATAATACCGGTGTTACGTTGtgtttg                                                                                                                 >  1:604565/1‑275 (MQ=255)

                                                                                                                                                 cGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                                                                               <  1:85875/275‑1 (MQ=255)

                                                                                                                                                        ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATc                                                                                                                                                                                                                                                          >  2:1022222/1‑129 (MQ=255)

                                                                                                                                                          tgggATTTGGTCCGCCGTTTTTCGGGCGATTCGCGCTGTTTCTTCGGTCTGGGGTGCCTTGCAATCGGGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtata                                                                                                      <  1:978984/274‑1 (MQ=255)

                                                                                                                                                            ggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGt                                                                                                                                                                                                                                                      <  1:1022223/129‑1 (MQ=255)

                                                                                                                                                                ttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATACTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTCTGATTGGTTCATTTATAAAAccc                                                                                                >  1:289469/1‑275 (MQ=255)

                                                                                                                                                                  gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGTTTTTGCTCCGCCAGCTCCTTGAGGTCGCTGCGAATAGTCTGCGGGCTGACGGAGAAAGGCGCTACCAGCGCTTCGGTCCTGACATAACCCTGCTGGTTAACCAGTTCGATAATACCGTTGTGACCTTGTGTTTGGTTGTTTTATAAATCCCtt                                                                                              >  2:649845/1‑274 (MQ=255)

                                                                                                                                                                  gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTg                                                                                              >  2:908449/1‑275 (MQ=255)

                                                                                                                                                                    tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGa                                                                                            <  2:879312/275‑1 (MQ=255)

                                                                                                                                                                     ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCCGTTTAACCAGTTCGATAATATCGTTGTGACGTTGTTTTTGTTTCATTTATAAATCCCTGCaa                                                                                           >  1:637269/1‑275 (MQ=255)

                                                                                                                                                                       gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                                              >  1:775455/1‑238 (MQ=255)

                                                                                                                                                                           cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                                          <  2:775455/238‑1 (MQ=255)

                                                                                                                                                                              tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcg                                                                                  >  1:625686/1‑275 (MQ=255)

                                                                                                                                                                                    cGATGCGCTCTTTTTCTGCGGGCTGGGTGGCCTGGCTATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttcg                                                                            <  2:314763/275‑1 (MQ=255)

                                                                                                                                                                                        gggcTCTTTTTCTTCGGTCTGGTGTGCCTTCGAATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa                                                                        <  2:431332/273‑1 (MQ=255)

                                                                                                                                                                                             ctTTTTCTACGGTCTGGGTGGCCTTGCCGTCGGGCCGCGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTTACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                                                   <  1:800296/275‑1 (MQ=255)

                                                                                                                                                                                             ctTGTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCGGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                                                   <  1:161910/275‑1 (MQ=255)

                                                                                                                                                                                             agacagcttcGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGTAATGCAGCGCCGCACCGCCATGATGG‑CTCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                                                   <  1:862873/269‑1 (MQ=255)

                                                                                                                                                                                                  tctgcGGTCTGGGTGGCCTTGCGATCCGTCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCAATGATGGAG‑CAGG‑ATCAGGTTTTGCTACGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                                               <  1:649845/274‑1 (MQ=255)

                                                                                                                                                                                                              gTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGATGTTTAACAAGATCGATAATACCGTTGTGAAGTTGGGTTTGTTTCATTTATAAATCCCAGGAATTATTTTAGTTTTCGCGCATTAAGCGAATcaacaactgtct                                                  >  2:862872/1‑269 (MQ=255)

                                                                                                                                                                                                                   cTTGCGATCGTGCTACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATACATCCCTGGAATTATTTTCGTTTTTGCGCATTTAGCGAATCAACAAAAGCCAACGCt                                             >  2:824639/1‑275 (MQ=255)

                                                                                                                                                                                                                       cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACGCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGGTGTGTTCGGTTCATTTATAAATTCATGGAATTATTTTAGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAtgtctct                                           >  2:888227/1‑267 (MQ=255)

                                                                                                                                                                                                                           cGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                                              <  1:888228/250‑1 (MQ=255)

                                                                                                                                                                                                                               ccACGGCGTGTTAGCCGAACTGGCAGGCAGAGCTGCACAGCCATGAGGG‑CGCAGG‑ATGAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGATGGAGAAATGCTCTACCAGATCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGGGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGc                                  <  1:414565/274‑1 (MQ=255)

                                                                                                                                                                                                                                cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑cg                                                                                                                                                                                                                                                                  <  1:451673/50‑2 (MQ=255)

                                                                                                                                                                                                                                  cggcggGGTAACCGAACTGGAAGGCAGGGCCGCACCGCGATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc                              <  2:680319/275‑1 (MQ=255)

                                                                                                                                                                                                                                   ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc                                                                  >  1:681446/1‑238 (MQ=255)

                                                                                                                                                                                                                                   ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACCAAAGCCATCGCTAAACCAACGGCTAAc                              >  1:443908/1‑274 (MQ=255)

                                                                                                                                                                                                                                       tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                                              <  2:681446/238‑1 (MQ=255)

                                                                                                                                                                                                                                               gAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgt                  >  1:822255/1‑274 (MQ=255)

                                                                                                                                                                                                                                                 aCTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAACACCATCGATCAACCAACGGCTAACCCGGCGATGTGTGCt               >  2:809326/1‑275 (MQ=255)

                                                                                                                                                                                                                                                          cAGCGCCGCACCGCCATGATGG‑CGCAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCCCGGCTAACCCGGCCATGTGTGCACCGTTCGcc      >  2:666642/1‑275 (MQ=255)

                                                                                                                                                                                                                                                               cccaaaccccATGCTGG‑C‑GCGGAATCAGGGTTGTCGCCCCCAGCTCATGGATGTCGCGCCGAATAGTCTGCGGGCTAACGGACAAATGCTTGACCAGCTCTTCGGTACTGACATAACACTGCTGTTTAACCAGTTCGTTAATACCGTTGTGACGTTTTGTTTGTTTCATTTATAAACCCCTGGAATTATTGTTGTTTTCGCGCATTGATCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGACATCg  <  2:856813/266‑1 (MQ=255)

CACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGG‑ATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCG  >  NC_000913/3560183‑3560710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATC  >  NC_000913/3560162‑3560751

CATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCTCCATGAT‑GG‑Cgcaggaa                                                                                                                                                                                                                                                                                                    >  M01186:109:000000000‑A8RG1:1:2105:5426:19393/1‑294 (MQ=60)

     CGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCTATGCGATCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCACGAT‑GG‑CGCAGGATCAGGG                                                                                                                                                                                                                                                                                               >  M01186:109:000000000‑A8RG1:1:2115:27677:15203/1‑301 (MQ=60)

      GGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGT                                                                                                                                                                                                                                                                                               <  M01186:109:000000000‑A8RG1:1:2108:12344:11169/300‑1 (MQ=60)

          GCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTG                                                                                                                                                                                                                                                                                           >  M01186:109:000000000‑A8RG1:1:1111:12382:9257/1‑300 (MQ=60)

            ttaagtgGCCACCGGAGATAATTATGCGAAAAGCTTCTTTTTGCATCAACTGGTTAGCAACGTTGAGACGGTTGGTGAGAATGCGCAAATTGCTGGGATTGGTCAGTGCGTGCGCTACCGCTTCTGGCGTG‑GTGGCTAGATCGATAAACATCGTCGAGCCATTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCGCTTTTTCGTTGGTCTGGGTGGCCTTGCTATCGGGGCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTC                                                                                                                                                                                                                                                                                        <  M01186:109:000000000‑A8RG1:1:1117:2350:9079/294‑1 (MQ=60)

                         GGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTGAT‑GG‑CGCAGGATCAGGTTTTGCCCCGCCAGCTCATTG                                                                                                                                                                                                                                                                           >  M01186:109:000000000‑A8RG1:1:1115:28449:10642/1‑301 (MQ=60)

                         GGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATAGTGGGTGACAATGCGCACATTGCTGTGATTGAGCAGTGCGTGCGCTACAGCTTCCGGCGTG‑GGGCCGATATCGATAAACAGCGGCGAGCCATGGGGGATTTGCTCCGCCACTTTGCGGGAGATGCGCTCTTTTTCTTCGGTCTGGGTGCCATGGCGAGCGTGCCACGGGGTGTTAACCGAACTGGTCGGCAGCGCCGCACCGCCGTGAT‑GG‑Cgccggttccggttttgctcgcccggctcattg                                                                                                                                                                                                                                                                           >  M01186:109:000000000‑A8RG1:1:1101:6902:6318/1‑269 (MQ=60)

                          gggatgatgagacgaaaagcgtatgtgaccatcaactgtgTAGCAACGTGGAGATTGTGGGTGACAATGCGCAAAGTGCTGTGATTGAGCGGTGCGTGCGCTAACGCTTCCGGCGTG‑TTGCCGATATCGATAAACAGGTTCGATGCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CTCAGGATCAGGTTTTGCTCCGCCAGCTCATTG                                                                                                                                                                                                                                                                           <  M01186:109:000000000‑A8RG1:1:2102:26251:21828/260‑1 (MQ=60)

                          GAGAGGATGATGCTAAAATGTTCTTGTAGCATTAACGTGTTAGCAACGTTTAGAGTGTGGGTGACAAGGCGCAAATGGCGGTGATTGAGCAGGGCGTGCTCTACCGCGCCCGGCGTC‑GGGCCGATATAGATAAACAGCGTCGAGCCATTGGGGATTTTCTCCGCCACTTTGCGGGCGATGCGCGCTTTGTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGTCGTGTTAACCGAACTGGAATGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCATCTCATTG                                                                                                                                                                                                                                                                           <  M01186:109:000000000‑A8RG1:1:2102:6444:23545/300‑1 (MQ=60)

                               AATGTTGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGGGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGT                                                                                                                                                                                                                                                                       <  M01186:109:000000000‑A8RG1:1:1103:18028:18179/299‑1 (MQ=60)

                               AATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGT                                                                                                                                                                                                                                                                       <  M01186:109:000000000‑A8RG1:1:2109:12581:11054/299‑1 (MQ=60)

                               AATGATGCGAAAATCTTCTTGTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGGGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGT                                                                                                                                                                                                                                                                       <  M01186:109:000000000‑A8RG1:1:1113:20368:3642/299‑1 (MQ=60)

                                   ATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGG                                                                                                                                                                                                                                                                  >  M01186:109:000000000‑A8RG1:1:2113:9534:13827/1‑300 (MQ=60)

                                      CGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCCGCCCATTGAGGTCGGGGCGAA                                                                                                                                                                                                                                                              >  M01186:109:000000000‑A8RG1:1:1107:16317:2621/1‑301 (MQ=60)

                                                  TTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGAACAGGT                                                                                                                                                                                                                                                                                               >  M01186:109:000000000‑A8RG1:1:2117:23360:3640/1‑256 (MQ=60)

                                                        ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGG                                                                                                                                                                                                                                            >  M01186:109:000000000‑A8RG1:1:2106:7444:9468/1‑301 (MQ=60)

                                                                        CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAG                                                                                                                                                                                                                            >  M01186:109:000000000‑A8RG1:1:1112:13170:10908/1‑301 (MQ=60)

                                                                             AGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATCGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTT                                                                                                                                                                                                                       >  M01186:109:000000000‑A8RG1:1:1114:17514:9598/1‑301 (MQ=60)

                                                                              GATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCGTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGCTCTTT                                                                                                                                                                                                                      >  M01186:109:000000000‑A8RG1:1:2111:9929:8288/1‑301 (MQ=60)

                                                                                  GTTGGTGACAATGCGCAAATTGGTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG                                                                                                                                                                                                                                                                           <  M01186:109:000000000‑A8RG1:1:2119:7851:17341/244‑1 (MQ=60)

                                                                                  GTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTA                                                                                                                                                                                                                  >  M01186:109:000000000‑A8RG1:1:2111:17181:19103/1‑301 (MQ=60)

                                                                                  GTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCT                                                                                                                                                                                                                          <  M01186:109:000000000‑A8RG1:1:1117:20997:16686/293‑1 (MQ=60)

                                                                                   TTGTTGAGAATGCGCAAATTGCTGTGATTGAGCAGTGGGGGCGGTACCGCTTCCGTGGTG‑GTGCTGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATTCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAC                                                                                                                                                                                                                 <  M01186:109:000000000‑A8RG1:1:2109:13287:10162/301‑1 (MQ=60)

                                                                                   TTGGTGACAATGCGCAAATTGCTGTGAGTGAGCAGTGCGTGCGCTACCGCTTCGGGGGTG‑GTGCGGATATCGAGAAACAGCGTCGACGCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAC                                                                                                                                                                                                                 <  M01186:109:000000000‑A8RG1:1:1119:22387:16334/301‑1 (MQ=60)

                                                                                         ACAATGCGCAAATTGCTGTGATTGAGCAGTGCGGGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA                                                                                                                                                                                                            <  M01186:109:000000000‑A8RG1:1:2116:20089:10938/300‑1 (MQ=60)

                                                                                               CGCAAATTGCTGTGATTGGGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCC                                                                                                                                                                                                      <  M01186:109:000000000‑A8RG1:1:1105:14271:13942/300‑1 (MQ=60)

                                                                                                        CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAA                                                                                                                                                                                            >  M01186:109:000000000‑A8RG1:1:1101:16037:22032/1‑301 (MQ=60)

                                                                                                           TGTTTGAGCGGTGCTTGCGGTACCGCTCCCGGCGTG‑GTGTCTATTTGGATAAACAGACTGGAGCCATTGGGGCTTTGCTCCGCCCCTTTTGGGGCGATTCGGTCTTTTTCTTCGGTCTGTGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACC                                                                                                                                                                                          <  M01186:109:000000000‑A8RG1:1:1105:7516:12733/300‑1 (MQ=60)

                                                                                                            cggtggagatgtccgtgcgcaccctctgtcggagtggtgccgttgtgGATAAACAGCGTCTGGCCATTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCCCTCTTTTTCTTCTGTCTGGGTGGCCTTGCAATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCTGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACC                                                                                                                                                                                          <  M01186:109:000000000‑A8RG1:1:2105:12133:22542/253‑1 (MQ=60)

                                                                                                             ATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCACTGGGGATTTGCCCCGCAACTTTGCGGCCAATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACCGGAAGGCAGCTTCGCACCGCCCAGAT‑GG‑CGCGGGATCAGGTTTTGCTCCGCCAGCTCATggcggtcgggacgaataggcctgggggtcacggtgacatgcccttccagctcttcggttctgacgtaacaccgctgttgaactcgt                                                                                                                                                                                       >  M01186:109:000000000‑A8RG1:1:1118:6584:3263/1‑215 (MQ=60)

                                                                                                                GAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAAATAACCCGGCTGTTTAACCCGTTGG                                                                                                                                                                                    >  M01186:109:000000000‑A8RG1:1:1111:7265:10211/1‑301 (MQ=60)

                                                                                                                     GTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCCGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACAAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGgtcgttaaa                                                                                                                                                                               >  M01186:109:000000000‑A8RG1:1:1110:21755:15150/1‑292 (MQ=60)

                                                                                                                     GTGCGTCCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACC                                                                                                                                                                                                       <  M01186:109:000000000‑A8RG1:1:1112:27429:19624/277‑1 (MQ=60)

                                                                                                                         cgttgcttcccgcttccgccgtggtgccgttatcgataacccgccttgttcctttgcggattggcgccgcctatttgctggcgatgctggCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCTCCGCACCGCCCTTAT‑GG‑CGCAGGATCAGGTTTTTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGATATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC                                                                                                                                                                             <  M01186:109:000000000‑A8RG1:1:2103:18610:3241/210‑1 (MQ=60)

                                                                                                                               TACCGCTTCCGGCTTGTGTG‑CGATATCTATAAACAGCGTCGGGCCGTTGGGGATTTGCTCCTCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACTGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA                                                                                                                                                                     <  M01186:109:000000000‑A8RG1:1:1113:8104:7941/301‑1 (MQ=60)

                                                                                                                                 CCGCTTCGGGCGTG‑GTGCCGATATCGATAAAGAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACG                                                                                                                                                                   <  M01186:109:000000000‑A8RG1:1:1104:14616:19656/301‑1 (MQ=60)

                                                                                                                                             TG‑GTGCGGATGTCGATAAACAGCGTCGAGCCATTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTT                                                                                                                                                        <  M01186:109:000000000‑A8RG1:1:1109:25615:6067/300‑1 (MQ=60)

                                                                                                                                             TG‑GTGCCGAGATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTT                                                                                                                                                        <  M01186:109:000000000‑A8RG1:1:1103:13988:7686/300‑1 (MQ=60)

                                                                                                                                                GTGCCGATATCGATAAACGGCGTCGAGCCATTGGGGATTTGGTGCGCCACTTGGCGGGCGATGCGGTCTTTTTCTTCGGTCTGGGTGGCCTTGCGGTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGGACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACC                                                                                                                                                                            <  M01186:109:000000000‑A8RG1:1:1113:15065:5712/278‑1 (MQ=60)

                                                                                                                                                GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTG                                                                                                                                                                                                 >  M01186:109:000000000‑A8RG1:1:1118:14593:16723/1‑257 (MQ=60)

                                                                                                                                                        TTCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAA                                                                                                                                             <  M01186:109:000000000‑A8RG1:1:1103:5030:19788/301‑1 (MQ=60)

                                                                                                                                                          CGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCATACCAGCGCTTCGGTACCGCCATAACCCTGCTGTTTAACCAGTTCGATAATACCGGTGTTACGTTGTGTTTGTCTCATTTACAAATT                                                                                                                                           >  M01186:109:000000000‑A8RG1:1:2102:24606:23384/1‑301 (MQ=60)

                                                                                                                                                                 ctgcgtcggcccgtttGGGATTTGGTCCGCCGTTTTTCGGGCGATTCGCGCTGTTTCTTCGGTCTGGGGTGCCTTGCAATCGGGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAA                                                                                                                                    <  M01186:109:000000000‑A8RG1:1:2116:8134:6399/285‑1 (MQ=60)

                                                                                                                                                                   GCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCC                                                                                                                                                                                                                                                                                    <  M01186:109:000000000‑A8RG1:1:2117:15088:20773/155‑1 (MQ=60)

                                                                                                                                                                           CCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATACTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTCTGATTGGTTCATTTATAAAACCCCGGGAGTATTTTCGT                                                                                                                          >  M01186:109:000000000‑A8RG1:1:1110:3446:13055/1‑301 (MQ=60)

                                                                                                                                                                                GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCCGTTTAACCAGTTCGATAATATCGTTGTGACGTTGTTTTTGTTTCATTTATAAATCCCTGCAATTATTTTCGTTTTCG                                                                                                                     >  M01186:109:000000000‑A8RG1:1:2104:24280:4252/1‑301 (MQ=60)

                                                                                                                                                                                  GGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTTTT                                                                                                                                                        >  M01186:109:000000000‑A8RG1:1:2108:20722:18991/1‑264 (MQ=60)

                                                                                                                                                                                         CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAG                                                                                                            >  M01186:109:000000000‑A8RG1:1:2103:8924:16763/1‑301 (MQ=60)

                                                                                                                                                                                                    tgagatttttataagagacagCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGTAATGCAGCGCCGCACCGCCATGAT‑GG‑CTCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAA                                                                                                 <  M01186:109:000000000‑A8RG1:1:2111:26873:21086/280‑1 (MQ=60)

                                                                                                                                                                                                    TGGGGGCGATGCTCTCTTTTTCTACGGTCTGGGTGGCCTTGCCGTCGGGCCGCGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTTACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAA                                                                                                 <  M01186:109:000000000‑A8RG1:1:2109:18630:16336/301‑1 (MQ=60)

                                                                                                                                                                                                    TGCGGGCGATGCGCTCTTGTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCGGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAA                                                                                                 <  M01186:109:000000000‑A8RG1:1:1106:23227:9367/301‑1 (MQ=60)

                                                                                                                                                                                                         ggcatggGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCCGTCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCAATGAT‑GGAG‑CAGGATCAGGTTTTGCTACGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGC                                                                                             <  M01186:109:000000000‑A8RG1:1:2104:17856:13432/293‑1 (MQ=60)

                                                                                                                                                                                                                                  GGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCC                                                                                            <  M01186:109:000000000‑A8RG1:1:2112:26747:18272/276‑1 (MQ=60)

                                                                                                                                                                                                                                      ggccgtgcgatgtgGCCACGGCGTGTTAGCCGAACTGGCAGGCAGAGCTGCACAGCCATGAG‑GG‑CGCAGGATGAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGATGGAGAAATGCTCTACCAGATCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGGGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGA                                                                <  M01186:109:000000000‑A8RG1:1:1114:3840:18755/286‑1 (MQ=60)

                                                                                                                                                                                                                                       GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGT                                                                                                                                                                                                                                                                                               <  M01186:109:000000000‑A8RG1:1:1116:13909:4884/76‑1 (MQ=60)

                                                                                                                                                                                                                                              GATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCC                                                                                            >  M01186:109:000000000‑A8RG1:1:2105:25270:14564/1‑264 (MQ=60)

                                                                                                                                                                                                                                              GATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACCAAAGCCATCGCTAAACCAACGGCTAACCCGGCGATGcatgat                                                        >  M01186:109:000000000‑A8RG1:1:1115:7321:19670/1‑294 (MQ=60)

                                                                                                                                                                                                                                                          GCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCAAC                                            >  M01186:109:000000000‑A8RG1:1:2110:10126:12125/1‑300 (MQ=60)

                                                                                                                                                                                                                                                            GTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAACCTGCTGTTTAACCAGTTCGATCATACCGTTGTGACGGTGTGTGTGTTTCAGTTATCAATCCCTGGAATTGTTTTCGTTTTCGCGCATTGAGCGAATCAAAAAAAGCCATCGCTCAACCCCCGGCTAACCTGGCGACGTGTGATCCGTTCGACATCAA                                          >  M01186:109:000000000‑A8RG1:1:2114:6957:10805/1‑300 (MQ=60)

                                                                                                                                                                                                                                                                                        CACCGCCATGATGGG‑G‑AAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGA             <  M01186:109:000000000‑A8RG1:1:2113:24974:12773/301‑1 (MQ=60)

                                                                                                                                                                                                                                                                                          CCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATGACCCTGCTGTTTAACCAGTTAGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTCCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGTCTAACCCcgcgctgtgtctgtctcttatccacatgtccgagcccacgcgacccgagagcacctcg           >  M01186:109:000000000‑A8RG1:1:2119:2772:12800/1‑243 (MQ=60)

                                                                                                                                                                                                                                                                                           CGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAG                                                                                                                                                                                        <  M01186:109:000000000‑A8RG1:1:1106:11011:19215/127‑1 (MQ=60)

                                                                                                                                                                                                                                                                                                   T‑GG‑CGCAGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTCGCTGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTTTTTAACCAGTTCGATAATCCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGTCGATGTGTGCTCCGTTCGCCA‑CGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATC  <  M01186:109:000000000‑A8RG1:1:1109:5293:13680/300‑1 (MQ=60)

CATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTG‑GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATC  >  NC_000913/3560162‑3560751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`