Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F9 I0 R1	177	96.4	2178490	87.4%	1904000	226.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,560,455:1	+G	100%	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	95.4%	193.0 / ‑0.9	65	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (47/15); total (50/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACC  >  NC_000913/3560218‑3560672

aTCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCCTGTT‑G‑Gc                                                                                                                                                                                                                            >  1:408222/1‑238 (MQ=255)

         ttAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGACCAg                                                                                                                                                                                                                  >  1:230400/1‑239 (MQ=255)

                cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCGGGATCAGGTTTTg                                                                                                                                                                                                            >  1:371805/1‑238 (MQ=255)

                   tGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGATCTGGGTGGCCTTGTGGTCGGG‑CCACGCGGTGTTAACCGGACTGGAAGCGCGCG‑CCG‑CCCCGCCATGCTGG‑GC‑CACGGCCAGGTTTTGCTcc                                                                                                                                                                                                        >  2:207805/1‑239 (MQ=255)

                           ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGGG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                >  1:146412/1‑239 (MQ=255)

                           ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACGGCCATGAT‑G‑GCGCAGGACACGGGTTTGCTCCCCCAGccc                                                                                                                                                                                                >  1:25515/1‑237 (MQ=255)

                           ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGcacgct                                                                                                                                                                                                 >  1:926063/1‑233 (MQ=255)

                               tGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGCGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGACCAGGTTTTGCTCCGCCAGCCCAtta                                                                                                                                                                                            >  1:23975/1‑238 (MQ=255)

                                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCCGCG‑CCG‑CCCCGCCATGAG‑G‑GCGCAGGGCCAGGTTTTGCCCCGCCAGCCCATTGGGGGcg                                                                                                                                                                                      >  2:639033/1‑239 (MQ=255)

                                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                      >  1:193681/1‑239 (MQ=255)

                                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGACCAGGTTTTGCTCCCCCAGCCCATTGGGGGcg                                                                                                                                                                                      >  2:601828/1‑239 (MQ=255)

                                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCC‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                       >  1:172146/1‑238 (MQ=255)

                                                cTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGGGGGGAAAAg                                                                                                                                                                            >  2:378709/1‑238 (MQ=255)

                                                  gtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGGGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAg                                                                                                                                                                            >  1:840054/1‑236 (MQ=255)

                                                          gCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGGGGTCGCGGCGAAAAGTCTGCGGGCTg                                                                                                                                                                 >  1:363446/1‑239 (MQ=255)

                                                              tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑GCCC‑CGGGGTCAGGTTTTGCTCCGCCAGCCCATTGGGGGCCCGGCGAATAGTGTGGGGGCTGACgg                                                                                                                                                             >  2:588318/1‑239 (MQ=255)

                                                               gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGCGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCCTGATGG‑GG‑CAGGGCCAGGTTTTGCCCCGCCAGGCCATTGGGGTCGCGGCGAAACGTCTGGGGGcgtggggg                                                                                                                                                            >  2:119791/1‑232 (MQ=255)

                                                                cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGCAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCACCCCATTGAGGTCCCGGCGAATAGTCTCCGGGCTGACGgag                                                                                                                                                           >  1:405478/1‑239 (MQ=255)

                                                                cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                           >  1:1032872/1‑239 (MQ=255)

                                                                cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCACCG‑CCG‑CCCCGCCATGAT‑G‑CCGCGGGATAAGGTTTTGCTCCGCCACCGCATTGAGGCCGCGGCGAATAGCCTGGGGGCTGGCGgag                                                                                                                                                           >  2:712952/1‑239 (MQ=255)

                                                                   gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                              >  1:432817/1‑233 (MQ=255)

                                                                        aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCGGCCCATTAAGGTCGCGGCGAAAAGTCTGCGGGCTGCCGGAGCAATGc                                                                                                                                                     >  1:593407/1‑237 (MQ=255)

                                                                        aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGGGGGCTGACGGGGAAATGc                                                                                                                                                     >  1:1052287/1‑237 (MQ=255)

                                                                        aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGGGAAAAGTCTGCGGGCGGCCGGGGAAAGGcc                                                                                                                                                    >  2:1058587/1‑237 (MQ=255)

                                                                            cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCCCCAGCCCATTGAGGTCCCGGGGAAAAGTTTGGGGGGTGAGGGGGAAAGTCTCCAcc                                                                                                                                               >  2:765823/1‑239 (MQ=255)

                                                                               ccGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTACTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGCGGAATGCTATACCa                                                                                                                                              >  1:160715/1‑237 (MQ=255)

                                                                                    ggggggcgtatatCAAAAAACGGGGTCGAGCCTTTGGGGTTTTG‑CCCCCCCCCTTGGCGGGCAATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTG‑CCACGGGGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                        <  1:639033/229‑1 (MQ=255)

                                                                                       gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAATGCAGCG‑CCG‑CCCCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAAAATTCTCCGGGCTGCCGGGGAAATACTCTACAACCACTTCggg                                                                                                                                    >  2:457801/1‑238 (MQ=255)

                                                                                         gCCAATATGGAAAAACGGCGTCGGGCCTTTGGGGATTTC‑CTCCGCCACTTGGCGGGCGAGGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                   <  1:765823/238‑1 (MQ=255)

                                                                                             atatCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAACTGCTCTACCAGCCCTTCGGTCCTgcc                                                                                                                              >  1:802716/1‑237 (MQ=255)

                                                                                                 cGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCGGTACTGACATa                                                                                                                           >  1:1007436/1‑238 (MQ=255)

                                                                                                  gATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTGGGTCGCGGCGAATAGTCTGCGGGCTGACGGGAGAATGCTCTACCAGCTCTTCGGAACTAACATaa                                                                                                                          >  1:691442/1‑238 (MQ=255)

                                                                                                  gATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGCCATaa                                                                                                                          >  1:706372/1‑238 (MQ=255)

                                                                                                      aaCCGCGTCGTGCTTTTGGGGGTTTGTCCCCGCCTCTTGGGGGGGGTGGCGCTCTTTTTCTGCGGGGTGGGGGGCCTTGCGATCGTG‑CCACGGCGTGTAAACCGAACTGGAAGGCAGCG‑CCG‑CCCCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                      <  2:672874/239‑1 (MQ=255)

                                                                                                           cGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCCCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCGCTACCACCTCTTCGGTCCTGACCTAACCCTGCTGtt                                                                                                                >  1:1065782/1‑239 (MQ=255)

                                                                                                             tCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                   >  1:181856/1‑218 (MQ=255)

                                                                                                             tCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTCCGGGCTGCGGGAGAAATGCCTTACCCGCTCTTCGGCACTGACATAACCCTGCTGTTTa                                                                                                              >  2:251541/1‑239 (MQ=255)

                                                                                                                aGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTCCTGTTTAAcc                                                                                                           >  1:699526/1‑239 (MQ=255)

                                                                                                                   ctttGGGGTTTGT‑CCCCGCCCGTTGGGGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGGGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                         <  1:601828/236‑1 (MQ=255)

                                                                                                                            tttG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGCCATAACCCTGCTGGTTAAACAGTTCGaaaaa                                                                                                >  2:1003729/1‑237 (MQ=255)

                                                                                                                            tttG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAAGCCTCTACCGGCCCTTCGGAACTGACATAACCCTGCAGTTTAACCAGTTCGttaat                                                                                                >  1:1067179/1‑238 (MQ=255)

                                                                                                                             ttG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTTACATAAACCCGCGGTTTAACCAGGTCGaaaata                                                                                               >  2:7204/1‑238 (MQ=255)

                                                                                                                             ttG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTTACAAAACCCTGCTGTTTAACCTGtctcttataaa                                                                                              >  2:927307/1‑229 (MQ=255)

                                                                                                                                      ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCCGTAATACCGTtctg                                                                                       >  1:693774/1‑238 (MQ=255)

                                                                                                                                         gtttGGGGGGGATGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGGG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt                                                                                   <  1:215822/238‑1 (MQ=255)

                                                                                                                                         cttggggggCGATGCGCTTTTTTTCTGCGGTCTGGTGGGCCTGGCGATCGTC‑ACACGGCGTGTTAACCGAAGTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt                                                                                   <  1:470762/233‑1 (MQ=255)

                                                                                                                                            tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCAAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGGATAGTCTGCGGGCTGACGGAGGAATGCTCTACCCCCTCTTCGGTACTGCCCTAACCCTGCTGTTTAACCAGTTCGATAAAACCGTTGTGACGtttg                                                                                >  2:877474/1‑237 (MQ=255)

                                                                                                                                                   gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCCGCTGTTTAACCAGGTCGTAAAACCCGTTGGGACGTTGgggttgtt                                                                         >  2:788624/1‑239 (MQ=255)

                                                                                                                                                       ccctttttttCTGGGGTCGGGGGGGCTTTGCG‑GCGTGCCCAGGGCG‑GTGAACCCGACTGGGAGGGCGCGCCCG‑CACCGCCATGAT‑G‑GCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAt                                                                     <  2:405478/234‑1 (MQ=255)

                                                                                                                                                             ttttcgcgggTCGGGGTGGCCTGGCGATCGTG‑CCGCGGCTTGTTAACCGACCGGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                               <  1:119791/231‑1 (MQ=255)

                                                                                                                                                                  tgcGGTTTGGGTGGCTTTGGGCTCGTG‑CCAGGGCGTGTTAACCGAGCTGGAAGGCGGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCGGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATccc                                                          <  2:322936/237‑1 (MQ=255)

                                                                                                                                                                    cGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGGT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAAAACCGTTGTGACGTTGTGTTTTTTTCATTTTTAAATCCCTg                                                        >  2:413580/1‑239 (MQ=255)

                                                                                                                                                                      gTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTTTACCAGCCCTCCGGAACTGACATAACCCTGCTGTTTAACCAGGTCGATAAAACCGTTGGGACGTGTGGTTTTTTTCATTTTAAAATCCCCGGa                                                      >  2:353030/1‑239 (MQ=255)

                                                                                                                                                                        cggggTGGCCTTGGAATGGTG‑CCAGGGGGTGTTACCCGAACGGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGaa                                                     <  1:788624/236‑1 (MQ=255)

                                                                                                                                                                                ccTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTAttttcg                                            >  1:701772/1‑239 (MQ=255)

                                                                                                                                                                                     cGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                       >  1:396645/1‑239 (MQ=255)

                                                                                                                                                                                         cGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGAAAAAACCGTTGTGACGTTGGGGTTGTTTAATTTTTAAATCCCCGGAATAATTTTCGTTTTCgcgc                                   >  2:875367/1‑239 (MQ=255)

                                                                                                                                                                                          gTG‑CCACGGGGTGTTACCCGAACGGGAAGGCAGGG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa                                  <  1:378709/239‑1 (MQ=255)

                                                                                                                                                                                          gTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGGGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa                                  >  1:545583/1‑239 (MQ=255)

                                                                                                                                                                                                      ggtTAACCGAACGGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                        <  1:7204/237‑1 (MQ=255)

                                                                                                                                                                                                       gtTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTTGGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTATTTTCGTTTTCGGCCATTTAGCGAATca                      >  2:682661/1‑239 (MQ=255)

                                                                                                                                                                                                              gAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCCTTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa                >  1:838455/1‑238 (MQ=255)

                                                                                                                                                                                                                atgggAAGGCAGGC‑CCG‑CACCGCAAGTAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGGATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc             <  1:877474/236‑1 (MQ=255)

                                                                                                                                                                                                                  ttgggggCAGCC‑CCGCCCCCGCTCTGAT‑G‑GCGAAGGTTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGGTGGGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc             <  2:160715/233‑1 (MQ=255)

                                                                                                                                                                                                                          aGCG‑CCG‑CACCGCCATGAT‑G‑GCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa    >  1:84295/1‑238 (MQ=255)

                                                                                                                                                                                                                           ccg‑ccc‑cCCCGCCATGGT‑G‑GCGCAGGAACAGGTTTGGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc  <  2:1052287/238‑1 (MQ=255)

ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCG‑CCG‑CACCGCCATGAT‑G‑GC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACC  >  NC_000913/3560218‑3560672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAC  >  NC_000913/3560216‑3560675

CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGACCAGG                                                                                                                                                                                                                    >  M01186:129:000000000‑A5M8V:1:1106:21206:18585/1‑251 (MQ=60)

       CGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCGGGATCAGGTTTTGC                                                                                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:1110:12254:8089/1‑250 (MQ=60)

         ttttagatacgttgaagtgttgggtagcaaggcgaaatttgatgagattgaggagtgtgtgcgtacccgttgcgggcggggtgcgtatctctATAAAGAGCGTCGAGCTATTGGGAATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGGGGCCTTGCGATCGGGCCACGGGGTGTTAGCCGAACTGAATGGCAGGGCCGAACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTC                                                                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:1108:16197:4987/158‑1 (MQ=60)

                  CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCA                                                                                                                                                                                                  >  M01186:129:000000000‑A5M8V:1:1104:4930:17856/1‑251 (MQ=60)

                  CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATGATGG‑Cgcaggacacgggtttgctcccccagccca                                                                                                                                                                                                  >  M01186:129:000000000‑A5M8V:1:1101:17865:17487/1‑222 (MQ=60)

                  CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCACGCTC                                                                                                                                                                                                   >  M01186:129:000000000‑A5M8V:1:2110:26967:16910/1‑250 (MQ=60)

                      tgtgtcgccttgggggtctggggatgtttgataagagacaggggtgtgcgcttgcgtgtccggggtggtgcggatatggataaccggggtcgagcatTTGGGGTTTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG                                                                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:2111:16831:26646/153‑1 (MQ=60)

                      GAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGCGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGACCAGGTTTTGCTCCGCCAGCccattag                                                                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:1101:28212:16698/1‑244 (MQ=60)

                          ttgtgggggaaaaggcgaaatgtgctgtgattgggcggggcgtgcgtgcccgcttcgggggtggtgccgtatcCGATAACCAGCGTCGAGCCTTTGGGGATTTGGCCCGCCACTTGGCGGGCGGTGCGCTCTTTTTCTTCGGTCGGGGTGGCTTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGT                                                                                                                                                                                           <  M01186:129:000000000‑A5M8V:1:1111:22708:6997/177‑1 (MQ=60)

                            GTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGG                                                                                                                                                                                        >  M01186:129:000000000‑A5M8V:1:1105:13821:21031/1‑251 (MQ=60)

                            GTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCCCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCT                                                                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:1105:24597:10112/1‑250 (MQ=60)

                                     actactcatttgcgtgttactgagtggtgcgtgcgctaccgtgtccggtgtggtgccgtaaccgatgaacgtgtccgagcgtttggggattgcctcggccatTTTGCGGGCAATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGACCGGTCCCCGGGGTGTTACCCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAG                                                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:2108:10822:5407/149‑1 (MQ=60)

                                     aagggcgaaattgttggagttggacagtggtggcgctaccgttccgggcggtgtgccgatagcgaacaccgggcgggagcgtttgggaattgcctcggcatgtttgcgggcgttgcgcttttttgctgtggtcgggggggccttgagatctgGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAG                                                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:1111:24678:18054/99‑1 (MQ=60)

                                     aaggcgcaaattgcgtgggttgggaagcgggtgcgctccctttcccggaggggtgccgacacgaaaaaaaagcggCGAGCCGTTGGGGTTGTGCTCCGCCACTTGGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCAACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAG                                                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:1102:3919:10219/176‑1 (MQ=60)

                                      gcggctggggggctcggaattggttaaaagggacggcCCGCTCCCGGCGTGGTCCCAATTTCGAAAAACGGCGTCGAGCCTTTGGGGATTGGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAG                                                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:2105:17566:25553/213‑1 (MQ=60)

                                         CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGC                                                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:2108:18199:15767/1‑248 (MQ=60)

                                           CAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGTCCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCACCGAACCGCCATGCTGG‑CGCAGGACCAGGTTTgcgtcgcccggccaattgagggcggagcgaagagtcagcg                                                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:1114:12945:21098/1‑211 (MQ=60)

                                                 GCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGGGGTCGCGGCGAAAAGTCTGCGGGCTGA                                                                                                                                                                   >  M01186:129:000000000‑A5M8V:1:1109:18964:26472/1‑251 (MQ=60)

                                                       ATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCACCCCATTGAGGTCCCGGCGAATAGTCTCCGGGCTGACGGAGA                                                                                                                                                             >  M01186:129:000000000‑A5M8V:1:1111:15560:2563/1‑251 (MQ=60)

                                                       ATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGA                                                                                                                                                             >  M01186:129:000000000‑A5M8V:1:2113:21025:11587/1‑251 (MQ=60)

                                                          GAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGG                                                                                                                                                                >  M01186:129:000000000‑A5M8V:1:1111:15533:20284/1‑245 (MQ=60)

                                                               GTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCGGCCCATTAAGGTCGCGGCGAAAAGTCTGCGGGCTGCCGGAGCAATGCT                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:2102:23584:14815/1‑249 (MQ=60)

                                                               GTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGGGGGCTGACGGGGAAATGCC                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:2113:25226:23791/1‑249 (MQ=60)

                                                                 GCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGGTAAACAGCGTCGCGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGTCGTGTTAACCCAACTGGAAGGCAGCGCCGCACCGCCCCGCTGGCC‑CAGGATCAGGATTTACTCCGCCAGCTCATTGACGTCGCGGCGCATAGGCTGCGGGCCGCCGGGGAAATGATCTA                                                                                                                                                    >  M01186:129:000000000‑A5M8V:1:1108:24539:9695/1‑250 (MQ=60)

                                                                      CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTACTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGCGGAATGCTATACCAG                                                                                                                                                >  M01186:129:000000000‑A5M8V:1:1104:11202:27744/1‑249 (MQ=60)

                                                                            cgttcccggcggggtcccgatgccgaaaacCAGCGCCGAGCCTTTGGGGTTTTGCCCCGCCTCTTGGGGGGCGATGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGG                                                                                                                                        <  M01186:129:000000000‑A5M8V:1:2101:19362:10527/221‑1 (MQ=60)

                                                                               tgcggggggggggcggtatgcgaaaaccggggtggggcatTTGGGGTTTTGCCCCGCCCCTTGGCGGGCGATGCGCTTTTTTTCGTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTAAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAC                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2102:8734:21348/211‑1 (MQ=60)

                                                                                    GCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAACTGCTCTACCAGCCCTTCGGTCCTGCCA                                                                                                                                >  M01186:129:000000000‑A5M8V:1:2107:6415:17799/1‑251 (MQ=60)

                                                                                     gggggggcgtatatcaaaaaacgggGTCGAGCCTTTGGGGTTTTGCCCCCCCCCTTGGCGGGCAATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA                                                                                                                                <  M01186:129:000000000‑A5M8V:1:2103:27666:18371/225‑1 (MQ=60)

                                                                                     gaggggcccaaatagtgaaaagcggggcgggatattggggctgtgcccgccacatgtgggggcaatggcttctttttcttaggacagtgggccctgccgaccggtccacggcgggtaaaccaaacgggaaggaagggcggagccgcaATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGTCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA                                                                                                                                <  M01186:129:000000000‑A5M8V:1:1102:3997:10225/103‑1 (MQ=60)

                                                                                        GGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCGGTACTGACATAA                                                                                                                             >  M01186:129:000000000‑A5M8V:1:2112:24376:17522/1‑250 (MQ=60)

                                                                                         GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTGGGTCGCGGCGAATAGTCTGCGGGCTGACGGGAGAATGCTCTACCAGCTCTTCGGAACTAACATAAC                                                                                                                            >  M01186:129:000000000‑A5M8V:1:2104:11614:26128/1‑250 (MQ=60)

                                                                                         GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTgtctcttatacccatt                                                                                                                            >  M01186:129:000000000‑A5M8V:1:2101:22738:16233/1‑234 (MQ=60)

                                                                                         GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGCCATAAC                                                                                                                            >  M01186:129:000000000‑A5M8V:1:2105:10588:11313/1‑250 (MQ=60)

                                                                                         GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATAGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGACGCACCGCCATGATGT‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTGGCGGCGAATAGACTGCGGGCCAACGGATACACGCTCTACCCGCTCTTCGGTACTGGCATAAA                                                                                                                            >  M01186:129:000000000‑A5M8V:1:2102:15990:29147/1‑250 (MQ=60)

                                                                                          tcccgatgtggaaaaccaggtgggagcctttggggtttggccCCGCCCCTTTGCGGGCGATGCGTTCTTTTTCTCGGGTCGGGGTGGCCTGGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACC                                                                                                                           <  M01186:129:000000000‑A5M8V:1:1109:12906:18227/208‑1 (MQ=60)

                                                                                          ggccaatatggaaaaacgGCGTCGGGCCTTTGGGGATTTCCTCCGCCACTTGGCGGGCGAGGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACC                                                                                                                           <  M01186:129:000000000‑A5M8V:1:2106:24355:20174/232‑1 (MQ=60)

                                                                                          aaggagagtcgaaaaaccGCGTCGGGCCTTTGGGGTTTTGCCCCGCCCCTTGGCGGGCGATCCGCTTTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCC                                                                                                                          <  M01186:129:000000000‑A5M8V:1:1105:24213:17260/233‑1 (MQ=60)

                                                                                             cgatcacaaaaaacggggtcgaccctttggggttttgccccgccttTTTGCGGGCGTTGCGCTTTTTTTCTTCGGTCGGGGTGGCTTGGCGATCGGGCCGCGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGC                                                                                                                       <  M01186:129:000000000‑A5M8V:1:2103:15101:15891/205‑1 (MQ=60)

                                                                                               tgttaacaacgccgagctgagtttggggttgttgcttccgcctctttccgggcgcggtgtcttttgtcttaGGTCTGGGGGGCCTTGGGATCGTGCAACGGGTTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCTTGATGG‑CGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTG                                                                                                                     <  M01186:129:000000000‑A5M8V:1:1102:16441:5390/180‑1 (MQ=60)

                                                                                               aatgagataacaggcgcgggcctttgggggtttggccCCGCCACTTTGCGGGCGATGCGCTCTTTTCCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTG                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2102:25296:12171/214‑1 (MQ=60)

                                                                                                  TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCCCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCGCTACCACCTCTTCGGTCCTGACCTAACCCTGCTGTTT                                                                                                                  >  M01186:129:000000000‑A5M8V:1:2114:23139:11076/1‑251 (MQ=60)

                                                                                                    GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAC                                                                                                                                     >  M01186:129:000000000‑A5M8V:1:1105:14260:14969/1‑230 (MQ=60)

                                                                                                     ttttaaaagagccagccttgggggtttgcCTCCGCCCCTTTGCGGCCGAGGCGCTTTTTTTCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAC                                                                                                               <  M01186:129:000000000‑A5M8V:1:2110:13191:17524/222‑1 (MQ=60)

                                                                                                       AAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTCCTGTTTAACCG                                                                                                             >  M01186:129:000000000‑A5M8V:1:2105:23101:7551/1‑251 (MQ=60)

                                                                                                                cgtgcctgtgggattgtacgccgcaactcggcgggcatgccctttttttcctccgggtggggtgtcgtggcgatcgcgcaacgcgGTGTTAACCGAAGTGGAAGGCAGCGCCGCCCCGCCAGGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAA                                                                                                    <  M01186:129:000000000‑A5M8V:1:2113:25097:19357/166‑1 (MQ=60)

                                                                                                                 gcgcttttgggactttgaccggccctttggcgggcgagcgcttcgTTTTCTGCGGTCTGGGTGTCCTGGCGATCGTGCCACGGGGTGTTACCCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGATGTTTAACCAGTTCGATAA                                                                                                    <  M01186:129:000000000‑A5M8V:1:2101:15084:7714/205‑1 (MQ=60)

                                                                                                                   cccttaggggattcgccccgccccatggcggggctttcccttttgatgcccggacgggcgggctgtgcatccgtgccgcggctgtTTAACCCAACTGGAAGGCGGCGCCGCCCCGCCATGATGG‑CGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGGCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC                                                                                                 <  M01186:129:000000000‑A5M8V:1:2101:26509:6430/166‑1 (MQ=60)

                                                                                                                   GCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAAGCCTCTACCGGCCCTTCGGAACTGACATAACCCTGCAGTTTAACCAGTTCGTTAATA                                                                                                  >  M01186:129:000000000‑A5M8V:1:2114:10216:11942/1‑250 (MQ=60)

                                                                                                                    tatttgggtgtttgccccgccattttcgGGGCGATGCGCCCTTTGTCTTCGGTCGGGGTGGCCTTGCGATCGGGCCACGGCTTTTTAACCGAACTGGAGGGCAGGGCGGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC                                                                                                 <  M01186:129:000000000‑A5M8V:1:1110:7352:20000/222‑1 (MQ=60)

                                                                                                                    cctttggggtttgtccccgcccgttgggGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC                                                                                                 <  M01186:129:000000000‑A5M8V:1:2102:22005:19312/222‑1 (MQ=60)

                                                                                                                        tggggattttccccccaactttggggactATGCGCTCTTCTTCTTCGGTCAGGGTGGCCTTCGGATCCGGCCACGGCGGGTGAACCGAACGGGAAGGCCGCGCCGTACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTG                                                                                            <  M01186:129:000000000‑A5M8V:1:2103:3162:17909/222‑1 (MQ=60)

                                                                                                                            agtttcctccgccccttgcggggcgaggcgctttttttcttcggtcgagggggccttgggttgggGCCACGGCGTGTTAACCGAACGGAAAGGCAGCGCGGAACCGCCATGATGG‑CGCAGGATTAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA                                                                                         <  M01186:129:000000000‑A5M8V:1:2113:13472:21578/185‑1 (MQ=60)

                                                                                                                            GATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCCGTAATACCGTTCTGA                                                                                         >  M01186:129:000000000‑A5M8V:1:2105:11914:2481/1‑250 (MQ=60)

                                                                                                                                         tgtttggggggGATGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT                                                                           <  M01186:129:000000000‑A5M8V:1:1106:20950:11036/240‑1 (MQ=60)

                                                                                                                                         ccttgggGGGCGATGCGCTTTTTTTCTGCGGTCTGGTGGGCCTGGCGATCGTCACACGGCGTGTTAACCGAAGTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT                                                                           <  M01186:129:000000000‑A5M8V:1:1112:23619:22309/244‑1 (MQ=60)

                                                                                                                                                             tttttcgcgggtcgGGGTGGCCTGGCGATCGTGCCGCGGCTTGTTAACCGACCGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAAT                                                       <  M01186:129:000000000‑A5M8V:1:1103:17532:27836/237‑1 (MQ=60)

                                                                                                                                                                      GGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTATTTTCGT                                              >  M01186:129:000000000‑A5M8V:1:2105:13117:8894/1‑251 (MQ=60)

                                                                                                                                                                        gcgGGGTGGCCTTGGAATGGTGCCAGGGGGTGTTACCCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTT                                             <  M01186:129:000000000‑A5M8V:1:2107:23056:10663/247‑1 (MQ=60)

                                                                                                                                                                           GGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCG                                         >  M01186:129:000000000‑A5M8V:1:1110:13833:21415/1‑251 (MQ=60)

                                                                                                                                                                                GCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGGGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAT                                    >  M01186:129:000000000‑A5M8V:1:2101:14282:9951/1‑251 (MQ=60)

                                                                                                                                                                                          TGTGCCACGGGGTGTTACCCGAACGGGAAGGCAGGGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATC                          <  M01186:129:000000000‑A5M8V:1:1110:14294:11985/251‑1 (MQ=60)

                                                                                                                                                                                                   GCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCCTTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAG                  >  M01186:129:000000000‑A5M8V:1:2108:21663:15009/1‑250 (MQ=60)

                                                                                                                                                                                                     GGGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCC                <  M01186:129:000000000‑A5M8V:1:1101:9751:7927/250‑1 (MQ=60)

                                                                                                                                                                                                               AACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAC      >  M01186:129:000000000‑A5M8V:1:1103:11252:6502/1‑250 (MQ=60)

                                                                                                                                                                                                                                   CCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAC  <  M01186:129:000000000‑A5M8V:1:2108:15823:24902/234‑1 (MQ=60)

CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAC  >  NC_000913/3560216‑3560675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`