Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
43 |
55.2 |
1410130 |
81.3% |
1146435 |
215.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
3,560,455:1 |
+G |
100% |
intergenic (‑2/+1) |
glpR ← / ← glpR |
pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,560,455 | 1 | . | G | 96.4%
| 74.1
/ ‑4.5
| 27 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (21/5); total (22/5) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCC > NC_000913/3560229‑3560661
|
aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCCGCACCGCCAT‑GAGGGcg > 1:423568/1‑227 (MQ=255)
aCGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATAGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCCGCACGCCCAT‑GCTGGcgcag > 1:485732/1‑223 (MQ=255)
ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGcgccggat > 1:672036/1‑220 (MQ=255)
ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCAT‑GATGGCGCAGGAGCGGGtctgg > 1:98828/1‑223 (MQ=255)
tgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCAT‑GATGGCGCAGGATCAGGTTTTGCt > 1:540484/1‑228 (MQ=255)
cAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCCTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt > 1:556367/1‑228 (MQ=255)
ttGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGCAGGCAGGGCCCCCCCGCCATAGTGGGC‑CGGGATAGGGTTTTGCCCCGCCGCCCCCTTGGGGGCGCGGGGa > 2:222770/1‑228 (MQ=255)
gtgcgtgcGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt > 1:199225/1‑228 (MQ=255)
tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg > 1:691380/1‑228 (MQ=255)
tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCAACGGCGTGTTAACCGAAGTGGAAAGCAGCGCCGCACCGCCAG‑GATGGCGCAGAATAAGGTTTGGCTCCGCCAGCCCATGTGGGCCGCGGGGAATAGtacg > 2:201846/1‑225 (MQ=255)
gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTTACGGGGAAAtccctgcc > 1:354442/1‑220 (MQ=255)
aaaCGGCGTCCGGCCTTGGGGGATTTGCCCCGCCACGTGGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc < 1:222770/227‑1 (MQ=255)
tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCAT‑GATGGCGCGGGATCAGGTTTTGTTCCGCCAGCTCATTGAGGTCGCGGCGAAAAGTCTGCGGGCTGACGGGGCAAGGGCCTACCCGCTCTTCGGGCTTGACATaaa > 2:423339/1‑227 (MQ=255)
gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGACACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GCTGGCGCAGGATCCGGTTTTGATCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGGGGGCTGCCGGGGAAATTCTCTACCAGCTCTCCGGACCTGCCATAACCCCTGTGTTTAACCAAtt > 2:270163/1‑228 (MQ=255)
cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTACGGTCGCGGCGATTAGTCTGCGGCCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCGGTTTAACCAGTTc > 1:339024/1‑228 (MQ=255)
ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGGGAATAGTCTGCGGCCTGTCGGGGAAATGCTCTACCACCTCACCGGTCCTGACCGAAACCTGCTGTTTAACCCGTTcaagaa > 2:115913/1‑223 (MQ=255)
tggCGGGCGATGCGCTGTTTTTCTCGGGTCTGGGTGCCCGTGCGATCGTGCCACGGTGTGTTAGCCGAACTGGAGGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg < 1:115913/226‑1 (MQ=255)
ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAGGCTCTACCGGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCCGTTCGATAATACCGCTGGGACCTTGtgttt > 2:442282/1‑228 (MQ=255)
ctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGCGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGTTATTACCGTTTTGACGTTGtgttt > 1:461646/1‑228 (MQ=255)
tgcttgGGTCTGGGTGGCCTGGCGATCGGGCCCCGGGGTGTTAACGGAACTGGAAGGCAGCGCCGAACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt < 1:14363/225‑1 (MQ=255)
ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGTTGACGGAGAACTGCCCTACCAGCTCTTCGGTACTGCCATAACCCCGCTGTTTAACCAGTTCGCTAATACCCTTGTTACGTATTGTTTTTTTCCTTTTTAAATc > 2:182105/1‑228 (MQ=255)
ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTTACATAACCCTGCTGTTTAACCAGTTCGACAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTaaaac > 1:638548/1‑226 (MQ=255)
cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTTCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGGCATAACCCTGCTGTTTAACCAGTTCGGTCATACCGTTGTGACGTTGGTTTTGTTTCATTTATAAATCCCTGGa > 1:654995/1‑228 (MQ=255)
tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGCAATAACCCTGCCGTTTAACCCGTTCGATATACCCCTTTTTACGTTGTGTTTGTTTCCTTTATATATCCCTGGAATTTttttcgttttccggca > 2:34552/1‑223 (MQ=255)
tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTTTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgcc > 1:444955/1‑227 (MQ=255)
gAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCCTTGAGCg > 2:374195/1‑227 (MQ=255)
cAGCGCCGCACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAGTTATTTTCGTTTTCGCGCATTGAGCGCATCAACaaaa > 1:583555/1‑227 (MQ=255)
cgccgcACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATCGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 1:442282/227‑1 (MQ=255)
cgccgcACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt > 1:403243/1‑108 (MQ=255)
gccgcACCGCCAT‑GATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 2:403243/108‑1 (MQ=255)
|
AGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCC > NC_000913/3560229‑3560661
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC > NC_000913/3560216‑3560696
|
CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGG > M01186:129:000000000‑A5M8V:1:1105:15160:10660/1‑251 (MQ=60)
ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCCGCACCGCCATGAGGGCGCGGGATCAGGTT > M01186:129:000000000‑A5M8V:1:2103:22336:16605/1‑251 (MQ=60)
ACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATAGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGGGCCGCACGCCCATGCTGGCGCAGGATCAGGTTTTG > M01186:129:000000000‑A5M8V:1:2105:6090:15191/1‑250 (MQ=60)
TGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCCGGATCAGGTTTTGCTC > M01186:129:000000000‑A5M8V:1:2112:22449:17920/1‑250 (MQ=60)
CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATGATGGCgcaggagcgggtctggctcttatacaca > M01186:129:000000000‑A5M8V:1:1103:22447:26710/1‑222 (MQ=60)
GTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAT > M01186:129:000000000‑A5M8V:1:2107:18873:10806/1‑251 (MQ=60)
tgtgtggcgcaaagcgcaaattgtggttgattgacaaggcggggccgtcccgcttccgggtgggtgcgaattccgaaaaccgccgtggggcttTTGGGGTTTTGCTCCGCCATTTTGCGGGCTATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGT < M01186:129:000000000‑A5M8V:1:1104:19257:27963/157‑1 (MQ=60)
GTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCCTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCG > M01186:129:000000000‑A5M8V:1:2107:15981:22070/1‑251 (MQ=60)
gtgataaagggagcgggctgggggagtgcgtcggctcgcgctgcgggtgggggcgcgatacgtATAAACAGCGTCGATCTATTGGGAATTGGCGCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACGGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAG < M01186:129:000000000‑A5M8V:1:2103:11957:2169/187‑1 (MQ=60)
tgcgaaatgggctggcgagaggctggggcgctgttccctttgcgggtgggtgccctatcgcgtagaacagcgccggcctttgggggttttgcgccgcccgttcgcgggcgagtcgccttgTTTCTCCGGTCTGGGGGCCCTTGCGATCGGCCCACGGGTTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTC < M01186:129:000000000‑A5M8V:1:1114:3560:13528/131‑1 (MQ=60)
GTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGG > M01186:129:000000000‑A5M8V:1:1107:17111:9804/1‑251 (MQ=60)
TGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGA > M01186:129:000000000‑A5M8V:1:2113:17862:25279/1‑251 (MQ=60)
tgtgcaggtggtgtgtgcgtgtgacagtggttgtcgtttgtttccaagaaaaagccggcatacggagtcctcaggggtctcgggggctcggagaggtgtataagagccaGGCCTTGCGATCGTGCCAGGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACC < M01186:129:000000000‑A5M8V:1:1104:16611:28267/142‑1 (MQ=60)
CTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTTACGGGGAAATccctgccccttttacacat > M01186:129:000000000‑A5M8V:1:2101:15764:13457/1‑231 (MQ=60)
tccgggcgggggcccataacggaaaacagtgtgcggcccgttggggtttggccCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCGTCGGTCGGGGGGGCCTTGCGATCGGGCCACGGGGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAC < M01186:129:000000000‑A5M8V:1:2101:21005:21744/198‑1 (MQ=60)
gcggatttgggtaaacggcgtccggccttgGGGGATTTGCCCCGCCACGTGGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCC < M01186:129:000000000‑A5M8V:1:1108:12969:7705/220‑1 (MQ=60)
ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCCCGGCGAATAGTCTGCGGGCTGACGCGGAAATGCTCTcccaccccttggggcctcacctaaccctgctgtt > M01186:129:000000000‑A5M8V:1:1109:26202:11144/1‑217 (MQ=60)
TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATCCTCTACCtgtctcttttacccatctccgagcccacgaga > M01186:129:000000000‑A5M8V:1:1102:11374:23511/1‑219 (MQ=60)
aaaacagggtcgtccttttggagtttgGCTCCGCCCCTTTGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGACCGTGCCAGGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACC < M01186:129:000000000‑A5M8V:1:2106:24773:6754/224‑1 (MQ=60)
aatcttttgggtattggctccgcccattggcgggcgatgggctttttttcctggggcgGGGTGGCCTGGCGATGGTGCCACGGGTTGTTAACCGAACGGGAAGGCAGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAA < M01186:129:000000000‑A5M8V:1:2109:18094:10848/192‑1 (MQ=60)
ttgggggatctgctcagccctgttgcgggaggtgagcactttttctcaggtttggtgggtctggcaattgtgcgcgGGCGTGTTAACCTAACTGGAAGCCCGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCG < M01186:129:000000000‑A5M8V:1:2110:21056:27899/174‑1 (MQ=60)
TTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTACGGTCGCGGCGATTAGTCTGCGGCCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCGGTTTAACCAGTTCGATtactttctt > M01186:129:000000000‑A5M8V:1:1114:25171:22451/1‑242 (MQ=60)
ttttgccccgacctttgggggcggagtcgcttttttccttggggCTGGGTGGCCTTGCGATCGGGCCCCGGGGTTGTAACCGAACTGTAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACG < M01186:129:000000000‑A5M8V:1:1104:10072:10955/207‑1 (MQ=60)
TGCCCCGCCTCTTGGCGGGCGATGCGCTGTTTTTCTCGGGTCTGGGTGCCCGTGCGATCGTGCCACGGTGTGTTAGCCGAACTGGAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTG < M01186:129:000000000‑A5M8V:1:1104:4313:15012/251‑1 (MQ=60)
GGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGCGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGTTATTACCGTTTTGACGTTGTGTTTGTTTCATTTATA > M01186:129:000000000‑A5M8V:1:2104:27177:20604/1‑251 (MQ=60)
GAGGCGCTTTTTTGCTTGGGTCTGGGTGGCCTGGCGATCGGGCCCCGGGGTGTTAACGGAACTGGAAGGCAGCGCCGAACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAA < M01186:129:000000000‑A5M8V:1:1101:21765:13045/250‑1 (MQ=60)
TCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTTACATAACCCTGCTGTTTAACCAGTTCGACAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAAACCCTGGCCTTATT > M01186:129:000000000‑A5M8V:1:2110:17341:26251/1‑251 (MQ=60)
GTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTTCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGGCATAACCCTGCTGTTTAACCAGTTCGGTCATACCGTTGTGACGTTGGTTTTGTTTCATTTATAAATCCCTGGAGTTATTTTCGTT > M01186:129:000000000‑A5M8V:1:2111:17695:19741/1‑251 (MQ=60)
GTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTTTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCcttgacgcattca > M01186:129:000000000‑A5M8V:1:2104:9655:9993/1‑238 (MQ=60)
ACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGATCTAACAGATCTTCGGTACTGACATAACCCCGCTGTTTAACCAGTTCGATAATACCGTTAGGAAGTTGGGTTTGGTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGAGCCTTGGGCGAATAAACCAAAGCCAAGGCTA > M01186:129:000000000‑A5M8V:1:1103:26641:9636/1‑251 (MQ=60)
GAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAGTTATTTTCGTTTTCGCGCATTGAGCGCATCAACAAAAGCCATCGCTAAA > M01186:129:000000000‑A5M8V:1:2108:7830:21404/1‑250 (MQ=60)
cgTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATCGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACC < M01186:129:000000000‑A5M8V:1:2104:24041:8116/248‑1 (MQ=60)
CTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACC > M01186:129:000000000‑A5M8V:1:2102:10355:25379/1‑131 (MQ=60)
GCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGgat > M01186:129:000000000‑A5M8V:1:2105:5814:16970/1‑248 (MQ=60)
CATGATGGCGCAGGGTCAGGTTTTACGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC < M01186:129:000000000‑A5M8V:1:1103:16799:4762/251‑1 (MQ=60)
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CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC > NC_000913/3560216‑3560696
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| Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
