Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F17 I0 R1	98	90.7	2214160	82.3%	1822253	222.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,560,455:1	+G	100%	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	93.0%	155.9 / 1.7	57	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (36/17); total (39/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑C‑CA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  NC_000913/3560237‑3560687

tGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGCAGGCAGCGC‑CCCCCCGCCATGA‑T‑GG‑C‑GAGGGATCAGGTTTTGCTc                                                                                                                                                                                                                        >  2:709694/1‑238 (MQ=255)

        ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGCCAGCGC‑CGCACCGCCATGT‑T‑GGCC‑CA‑GGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                               >  1:1084737/1‑239 (MQ=255)

                  tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGTTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                     >  1:593008/1‑239 (MQ=255)

                  tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                     >  1:468644/1‑239 (MQ=255)

                  tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCCCCATGA‑T‑GG‑CGCC‑GGATCAGGTTTTGCTCCGCCCGCTCATTGCgggcc                                                                                                                                                                                                     >  1:1091085/1‑236 (MQ=255)

                              tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATCCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCAACGGCGTGTTAACCGAACGTGAAGGCAGCGC‑CGCCCCGCCTTGACG‑GG‑G‑CG‑GGATCAGGTTTTGCTCCCCCAGGCCATTGAGGGCGCGGGGaaaaagc                                                                                                                                                                                         >  2:940789/1‑234 (MQ=255)

                                     gAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                                  >  1:341413/1‑239 (MQ=255)

                                        cAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCC‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCCCGGCGAATAGTCTCGGGGCTGa                                                                                                                                                                               >  1:678605/1‑239 (MQ=255)

                                           tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                            >  1:739806/1‑239 (MQ=255)

                                            gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGTTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCCCCAGCTCCTTGGGGTCCGGGGGAAGAGTTTGCgggtggcggga                                                                                                                                                                           >  2:403473/1‑231 (MQ=255)

                                             cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCTC‑CGCACCGCCAGGA‑T‑GG‑CGCA‑GGATCAGGATTTGCTCCGCCAGCCCATTGAGGTCGGGGGGAAAAGTATGCGGGCGGACGgag                                                                                                                                                                          >  2:289619/1‑239 (MQ=255)

                                             cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                                          >  1:97596/1‑239 (MQ=255)

                                                  gcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                                                                                                                      >  1:934258/1‑238 (MQ=255)

                                                     aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGc                                                                                                                                                                    >  1:648702/1‑237 (MQ=255)

                                                     aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGCGGTGTTAACCGACCTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCCGGTTTTGTCCCGCCAGCCCATTGCGGCGCCGGCCAATAGTCCGGGGTCTGAGGGAGaaacacc                                                                                                                                                                   >  2:596627/1‑234 (MQ=255)

                                                     aCCGCTTCCGGCGGGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCAACGTCGGTGTACCCGAACTGGAAGGCAGCGC‑CGCACCGCACTGA‑T‑GG‑CGCC‑GGACAGGGTTTTGGTCCGCCAGCCCATTTAGGTGGCGGCCAAAGGTCTTCGGGCGGGGGgagagaagcgt                                                                                                                                                                  >  2:739639/1‑232 (MQ=255)

                                                         cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACAGGAAGGCAGCGC‑CGCACCGCACTGC‑T‑GG‑CGCA‑GGACCAGGTTTTGCTCCGCCAGCTCTTTGAGGCCGCGGCGCATAGGCAGCGGGCGGCCGGGGAAATGcatcacc                                                                                                                                                              >  2:986719/1‑233 (MQ=255)

                                                                       cgaatatCGATAACCAGCGTGGGCCCATTGGGGTTTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTCCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCCCCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                 <  1:596627/235‑1 (MQ=255)

                                                                               gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTTCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCGGTACTGACATaa                                                                                                                                         >  1:509099/1‑238 (MQ=255)

                                                                               gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa                                                                                                                                         >  1:1011474/1‑238 (MQ=255)

                                                                                       gCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACTTAACCctgctgg                                                                                                                                >  1:187887/1‑238 (MQ=255)

                                                                                        cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGtt                                                                                                                               >  1:950555/1‑239 (MQ=255)

                                                                                        cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GC‑CGCT‑GGACCAGGTTTTGCCCCGACAGCCCATTGAGGTCGCTGCGAATAGACTGCTGGCTCACGGAGAAATTCTCTACCAACCCTTCGGGACTTCCATaaaacgtgtgtt                                                                                                                               >  2:382667/1‑229 (MQ=255)

                                                                                           cGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                                                                                                                       >  1:345087/1‑228 (MQ=255)

                                                                                             aGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAAc                                                                                                                           >  1:975032/1‑238 (MQ=255)

                                                                                                ttttGGGGATTTGCCCCGCCACTTGGCGGGCGATGGCCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCGGGCGTTTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                        <  1:403473/236‑1 (MQ=255)

                                                                                                ctttGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGTGCCAGGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                        <  1:709694/236‑1 (MQ=255)

                                                                                                   tGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCC‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAATAGCCTGCGGGCTGGCGCGGAAATGCCCTACCAGCTCTTCGGAGATGACAAAAACCTGGTGTTTCACCAgcctg                                                                                                                    >  2:354411/1‑235 (MQ=255)

                                                                                                          ttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGGCATAACCCTGCTGTTTAACACGTTCGataata                                                                                                              >  1:136225/1‑238 (MQ=255)

                                                                                                          ttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                              >  1:999601/1‑238 (MQ=255)

                                                                                                          ttGCCCCGCCCTTTTGGGGGCGATGCGCTCTTTTCCTGCGTGCTGGGTGGCCTGGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                             <  1:384711/239‑1 (MQ=255)

                                                                                                                    tcTTTGGGGGCGTTGGGTTTTTTTTTTTGGGTCTGGTGGGCCTTGGGATCGGGCCACGGGGTGTTACCCGACCGGGAAGGCGGCGC‑CGCCCCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                   <  2:97596/238‑1 (MQ=255)

                                                                                                                             gCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACgtttggtttg                                                                                          >  1:1099394/1‑239 (MQ=255)

                                                                                                                               gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCGGTTTAACCAGTTCGATAATACCGTTGGGACGTTGtgtttgtt                                                                                        >  1:394634/1‑239 (MQ=255)

                                                                                                                                atggcttttttttCTTGGGGCTGGGGGGTCTTGCTGTCGTGCCCGGGAGTGTTAACAGAGTTGGAAGGCAGCGC‑CGCACCGCCATGG‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                                                        <  2:342880/230‑1 (MQ=255)

                                                                                                                                aTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGTCCATTGAGGTCGCGGCGGACATTCTGCGGGCGGGACGAGAAACGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTACCCAGTTCGATAAAACCGTTGTTGCGGTGGgtttgttt                                                                                       >  2:1077487/1‑239 (MQ=255)

                                                                                                                                      tttttttCTTGGGTCTGGGTGGCTTTGGGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                                      <  1:793963/232‑1 (MQ=255)

                                                                                                                                             ctgcggttgggggggCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATcc                                                                          <  1:739639/230‑1 (MQ=255)

                                                                                                                                                cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATTCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGCGAATACCGTTGTGACGTTGGGTTTTTTTCATTTATAAATcctgt                                                                       >  2:793963/1‑236 (MQ=255)

                                                                                                                                                cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAATCCCTg                                                                       >  1:656453/1‑239 (MQ=255)

                                                                                                                                                  gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGGTTAACCAGTTCGATAATACCGTTGTGCCTTTTTTTTTGTTTCCTTTTTAAATCCCTGGa                                                                     >  2:882792/1‑239 (MQ=255)

                                                                                                                                                                 cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCGCTTCGGTACTGACCTAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCCTTTATAAAACCCTGGAATAAttttcgttttt                                                      >  2:540916/1‑238 (MQ=255)

                                                                                                                                                                      ggtccACGGCTGGTTAACCGAACTGGAAGGCGGCGCGCGC‑CCGCCATGA‑T‑GG‑CGCA‑GGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa                                                 <  1:1045017/236‑1 (MQ=255)

                                                                                                                                                                      gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa                                                 >  1:788782/1‑239 (MQ=255)

                                                                                                                                                                      gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                              >  1:271386/1‑178 (MQ=255)

                                                                                                                                                                                ttgtAAACCAACCGGGAAGGCCGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                        <  1:540916/237‑1 (MQ=255)

                                                                                                                                                                                         gAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAGTTCGATAATACCGTTTGGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTATTTTCGTTTTCGGGCATTGAACGATACAaaaaaac                              >  2:368508/1‑238 (MQ=255)

                                                                                                                                                                                           ttgggATGGCCGCGT‑CGCTCCGCCATGG‑TGGG‑G‑AA‑GGGTACGGTTTTGGCGCGGCGGCTCATTGAGGTCGCGGCGAAGAGGCTGCGGGCTTGCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATGATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                            <  2:126270/236‑1 (MQ=255)

                                                                                                                                                                                             gggaagtcgcggc‑cgccCCGGCATGG‑T‑GG‑CGCA‑GGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa                           <  1:1077487/227‑1 (MQ=255)

                                                                                                                                                                                                     aGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                      >  2:684839/1‑107 (MQ=255)

                                                                                                                                                                                                     aGCGC‑CGCACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                      <  1:684839/107‑1 (MQ=255)

                                                                                                                                                                                                      gcgc‑CGAACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc                 <  1:1013508/239‑1 (MQ=255)

                                                                                                                                                                                                       cgc‑cgcACCGCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc                 <  1:289619/238‑1 (MQ=255)

                                                                                                                                                                                                             ccccGCCATGG‑T‑GG‑CGGA‑GGATCGGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGc           <  2:468644/237‑1 (MQ=255)

                                                                                                                                                                                                                 gCCATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTTATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATCCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc       <  1:904750/239‑1 (MQ=255)

                                                                                                                                                                                                                  ccATGA‑T‑GG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAAAAAAAGCCATCCCTAAACCCACGGCAAAc       >  2:940331/1‑238 (MQ=255)

                                                                                                                                                                                                                      ga‑t‑gG‑CGCA‑GTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc  <  1:354411/239‑1 (MQ=255)

TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGA‑T‑GG‑C‑CA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGC  >  NC_000913/3560237‑3560687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560216‑3560698

ccaccaagttgttgcgaagtttggattttttggtgaaaatggcaaaattcttggattggagcgggcggggccttcccgttcccgggGTGGTGCCGATACGGATAACCAGCGTGGGGCCTTTGGGGTTTTCCCCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGG                                                                                                                                                                                                                                           <  M01186:129:000000000‑A5M8V:1:2112:16638:21093/165‑1 (MQ=60)

 catcatcgtttttacacggtggagtttgtgtgtacgaatgcgcaatttgtgtgtatcGAGCAGTGCGTGACCTCCCGCTTCGGGCGTGGTGCGTATATCGGTAAACAGCGTCGAGCCTTTGGGGATTGGCTCCGCCACTTTGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGT                                                                                                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:1107:19391:11644/194‑1 (MQ=60)

 cataatggttttagcaaggttggatttgtgggtaacatggcgaaattggctgtgattgcgcagggcggccgctcccgttccgggcggGGTGCGGATATCGATAAACGGCGGCGAGCCTTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGT                                                                                                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:2110:17851:17004/164‑1 (MQ=60)

 cataagcgtgttagcaagtttgaatttgtgggtcaacATGCGAAAATTGCTGTGATGGAGCAGTGCCTGCGCTACCACTTCCGGCGGGGTCCCGATATCAATAATCAGCGCCGACCAATTGGGGATTTGTCCCCCCACTTTGCGGGGGTTGCCCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAGGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGT                                                                                                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:2106:9516:10446/214‑1 (MQ=60)

                 ACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGGCC‑CAGGATCAGGcctgtttctcttaccca                                                                                                                                                                                                                          >  M01186:129:000000000‑A5M8V:1:2114:11152:6647/1‑234 (MQ=60)

                  CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGCCAGCGC‑CGCACCGCCATGTTGGCC‑CAGGATCAGGTTTTGCTCCGCCAGCTCA                                                                                                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:2114:12585:14327/1‑251 (MQ=60)

                     ttgtgttgtgggggagagggcgaaattggttggtatgtgcaggtgcgggagctcccgtgcgcgggtggggccagtaaccgaaacacggcgtcgcgccttgggggttttgccccccccctttgcggcggttgccCTCTTTTGCTTCGGTCGGGGGGGCCTTGCGATCGAG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG                                                                                                                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1102:9901:4211/118‑1 (MQ=60)

                     tgagattgtgggggacatggggaaaatggttgggatggaggagtgcgggcgctcccgtttcggggggggggcgaatatcaaaaaccggcgtggggcctTTGGGGATTGGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTG                                                                                                                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1104:22137:15458/153‑1 (MQ=60)

                       ttttgtttcaaggaaaaagcgggcatacgagctaggccgggttctggggggctgggagatggtaaaaagagacagtcgaaaaacggcgcCGAGCCATTGGGGTTTGGCCCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAG                                                                                                                                                                                                                    <  M01186:129:000000000‑A5M8V:1:2104:13021:7310/162‑1 (MQ=60)

                          ttggtgggaaaagggggaatgggtggtgttttggccggggcgggcgtttcgtcttggcgcgtgggccctttatgaaatagcgggtcggcgtttttggggcttcgctcagcttCTTTGCGGGCAATGCCTTCTTTTTCTGCGGTCTGGGTGTCCTTGCGTGCGGG‑‑CCGCGGGGTTTAAACCGAACGGGAGGGCAGGGC‑CGAGCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGT                                                                                                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:2114:22004:14516/138‑1 (MQ=33)

                            GTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGTTCCGCCAGCTCATTGAGGTCGG                                                                                                                                                                                                               >  M01186:129:000000000‑A5M8V:1:2102:18964:4548/1‑251 (MQ=60)

                            GTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGG                                                                                                                                                                                                               >  M01186:129:000000000‑A5M8V:1:1112:20354:22583/1‑251 (MQ=60)

                            GTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCCCCATGATGG‑CGCCGGATCAGGTTTTGCTCCGCCCGCTCATTGcgggccg                                                                                                                                                                                                               >  M01186:129:000000000‑A5M8V:1:2114:28015:17511/1‑244 (MQ=60)

                                               TTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCG                                                                                                                                                                                            >  M01186:129:000000000‑A5M8V:1:1109:4616:20002/1‑251 (MQ=60)

                                                  CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCCCGGCGAATAGTCTcggggctgag                                                                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:2104:22790:12217/1‑241 (MQ=60)

                                                     TGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGG                                                                                                                                                                                      >  M01186:129:000000000‑A5M8V:1:2105:18913:24565/1‑251 (MQ=60)

                                                       ATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGA                                                                                                                                                                                    >  M01186:129:000000000‑A5M8V:1:1103:20422:15976/1‑251 (MQ=60)

                                                            GCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATG                                                                                                                                                                                >  M01186:129:000000000‑A5M8V:1:2110:24086:21073/1‑250 (MQ=60)

                                                               tggggtcgtgcccgcctgcggggtgggggcggaaagaggtaaacggcgcggggcatttgggattttgcccccgcctttgGCGGGCGATGCGCTCTTTTTCTGCGGTCGGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTTTTAACCGAACTGGAGGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTC                                                                                                                                                                             <  M01186:129:000000000‑A5M8V:1:1104:17822:21878/171‑1 (MQ=60)

                                                               gtgcgggcgttcccgcttcgggggtggtgcgaattccaaaaaccagggtcgggcgtTTGGGGATTTGCCCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTC                                                                                                                                                                             <  M01186:129:000000000‑A5M8V:1:2106:7456:14679/194‑1 (MQ=60)

                                                               GTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCT                                                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:2103:8011:16294/1‑249 (MQ=60)

                                                                  cgggcctcgccgcttcgggcggggggcggtttcggaaaaccagcgtcggcgttTTGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTTTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAC                                                                                                                                                                          <  M01186:129:000000000‑A5M8V:1:2111:20668:8137/197‑1 (MQ=60)

                                                                               tgcggggggggtgccgattcggataaccggcgcggacCCATTGGGTATTGGCTCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTCCGGTCTGGGTGGCCTTGCGATCGGG‑‑CCACGGGGTGTTAACCGACCTGAAAGGCGGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAC                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:2105:20310:23730/214‑1 (MQ=60)

                                                                                     gtggggcccgatatggagaaacggggtcgggccttgggggatttggccgGCCACTTGGCGGGCGATGCGCTCTTTTTCTCCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCAGGGCGTGTAAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACA                                                                                                                                                       <  M01186:129:000000000‑A5M8V:1:1107:3744:15026/201‑1 (MQ=60)

                                                                                        tggagctgtttgaaaaaaccgcgtaGAGCCATTGGGATTTTGCCCCGCCAGTTTGCGGGCCATGGCCTCTTTTTCTTCGCTCGTGGTGGCCTTGCGATCGGG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAC                                                                                                                                                   <  M01186:129:000000000‑A5M8V:1:2104:15426:28608/226‑1 (MQ=60)

                                                                                         GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTTCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCGGTACTGACATAAC                                                                                                                                                   >  M01186:129:000000000‑A5M8V:1:1113:13438:23005/1‑250 (MQ=60)

                                                                                         GTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAC                                                                                                                                                   >  M01186:129:000000000‑A5M8V:1:2112:25109:18144/1‑250 (MQ=60)

                                                                                          tgcgattgtgaaaaacgcgcttgggcctcttggggtttgcccCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCC                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2107:22402:11651/209‑1 (MQ=60)

                                                                                          ggcgtaattcaaaaaacgggtgccgccctttggggatttcccccgccatgtgGCGGGGGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTG‑‑CCAGGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACC                                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:1113:11253:24854/198‑1 (MQ=60)

                                                                                          gcccagtaacgaaaacgcgtgtaggccttttggggattggccccgcccgTTTGCGGGCGATGCGCTATTTTTCTGCGGTTTGGGGGGCCTTGCGATGGTG‑‑CCAAGGCGTGTTAACCAACCTGGAAGGCAGCCC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACC                                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:2106:6802:13345/201‑1 (MQ=60)

                                                                                          CGCCGATAACGATAAACAGGGTCGAGCATTTGGGGTTTTGCCCCGCCACTTGGCGGGCGAGGCCCTCTTTTTCTTCGGCCGGGGGGGCCTTGCGATCGTG‑‑CCACGGCGTTTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACC                                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:2110:26267:22443/250‑1 (MQ=60)

                                                                                           gcgagtgtggtaaaccggcgtcggtgttttggggatttgcgcgcccccttgggGGGCGGTGCGTTCTTTTTCTTCGGTCTGGGGGGCCTGGCGATCGGG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCC                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:1113:23733:18068/197‑1 (MQ=60)

                                                                                           gcgaATATCGATAACCAGCGTGGGCCCATTGGGGTTTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTCCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCC                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2102:26581:7455/246‑1 (MQ=60)

                                                                                           ccggatacgataaaaccggtcggagcttTTGGGGTTTTGCTCCGCCACGTGGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACAGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCC                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2112:15367:3860/222‑1 (MQ=60)

                                                                                                 ATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACTTAACCCTGCTGGT                                                                                                                                          >  M01186:129:000000000‑A5M8V:1:1105:15586:23090/1‑251 (MQ=60)

                                                                                                  TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTT                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:2111:16064:8050/1‑251 (MQ=60)

                                                                                                  TCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGACGCGGCGAATAGTCTGCCGGCTGACGGGGAAATGCTCTCCCACCTCTTCGTTACTGCCAAAACCCTGCTGTTT                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:2112:2599:12502/1‑251 (MQ=60)

                                                                                                    GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTATTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAATAGTCTCCGGGCTGACGCAGAAATGCTCTACCACCTCTTCGGAACTGACATAACC                                                                                                                                                  >  M01186:129:000000000‑A5M8V:1:2102:26955:10799/1‑240 (MQ=60)

                                                                                                     ATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCC                                                                                                                                                 >  M01186:129:000000000‑A5M8V:1:1109:26054:22141/1‑240 (MQ=60)

                                                                                                       AAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACC                                                                                                                                     >  M01186:129:000000000‑A5M8V:1:2111:5797:20726/1‑250 (MQ=60)

                                                                                                              atccgccttggttcggggggttcggagatgtgtaaaagaaccgGCTCTTTTTCTTCGGTCGGGGGGGCCTTGCG‑‑TGTGGTCCACGGGGTGTTAACCGAATTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGAT                                                                                                                             <  M01186:129:000000000‑A5M8V:1:1102:6833:18067/208‑1 (MQ=60)

                                                                                                                    cttTTGGGGATTTGCCCCGCCACTTGGCGGGCGATGGCCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCCGGGCGTTTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC                                                                                                                        <  M01186:129:000000000‑A5M8V:1:1111:10663:9378/247‑1 (MQ=60)

                                                                                                                    CCTTTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGTG‑‑CCAGGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC                                                                                                                        <  M01186:129:000000000‑A5M8V:1:2105:14310:7804/250‑1 (MQ=60)

                                                                                                                    CCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGGCATAACCCTGCTGTTTAACACGTTCGATAATAA                                                                                                                        >  M01186:129:000000000‑A5M8V:1:1104:29152:16009/1‑250 (MQ=60)

                                                                                                                    CCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC                                                                                                                        >  M01186:129:000000000‑A5M8V:1:2112:10265:12086/1‑250 (MQ=60)

                                                                                                                     ttttgggtttttcccccgcacCTTTGCGGGCGATGCGCTCTTTTTCGTCGGTCTGGGTGGCCTGGGTATCGTG‑‑CCACGGTGTGTTAACCGACCTGGAGGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCG                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1102:18075:16487/230‑1 (MQ=60)

                                                                                                                              TTTGCCCCGCCCTTTTGGGGGCGATGCGCTCTTTTCCTGCGTGCTGGGTGGCCTGGCGATCGGG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGT                                                                                                             <  M01186:129:000000000‑A5M8V:1:1110:17759:21212/251‑1 (MQ=60)

                                                                                                                               gtataagaaacagtttggcgggcgatgcgcgctttttcgtcggtcgggtgggccttgcgtcggtgcccggggGTGTTAACCGAACTGGAAGGCAGGGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAC                                                                                                               <  M01186:129:000000000‑A5M8V:1:1109:15812:4863/178‑1 (MQ=60)

                                                                                                                                tgctccccctctttggggggtatgccctcttttccttcggccggggggcCCTTGCGATCGTC‑‑CCCCGGCGTGTTAACCGACCTGGAAGGCAGCCC‑CGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTG                                                                                                           <  M01186:129:000000000‑A5M8V:1:2113:14965:2013/202‑1 (MQ=60)

                                                                                                                                       CCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTTGGTTTGT                                                                                                    >  M01186:129:000000000‑A5M8V:1:2114:25041:22040/1‑251 (MQ=60)

                                                                                                                                         ACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCGGTTTAACCAGTTCGATAATACCGTTGGGACGTTGTGTTTGTTT                                                                                                  >  M01186:129:000000000‑A5M8V:1:1111:15193:2288/1‑251 (MQ=60)

                                                                                                                                           cctgtcgggtcggtgggttgttttgcgtcggactgggtggccttgcggtcggccctggggGTGTTAACAGAGCTGGAAGGGAGCGC‑GGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTGGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTT                                                                                                  <  M01186:129:000000000‑A5M8V:1:1103:23173:23261/191‑1 (MQ=60)

                                                                                                                                                      cttggccttttttccctgggtcggggtggcctggcaatcggcccaggggGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAA                                                                                        <  M01186:129:000000000‑A5M8V:1:1114:9582:17389/201‑1 (MQ=60)

                                                                                                                                                          CTTTTTTTCTTGGGTCTGGGTGGCTTTGGGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATC                                                                                      <  M01186:129:000000000‑A5M8V:1:2107:15036:10848/246‑1 (MQ=60)

                                                                                                                                                          CTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAATCCCTGG                                                                                 >  M01186:129:000000000‑A5M8V:1:2103:9814:20700/1‑251 (MQ=60)

                                                                                                                                                                 tctgcggttgggggGGCCTGGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATT                                                                          <  M01186:129:000000000‑A5M8V:1:2105:6422:24427/237‑1 (MQ=60)

                                                                                                                                                                                GCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAT                                                           >  M01186:129:000000000‑A5M8V:1:2107:20152:7782/1‑251 (MQ=60)

                                                                                                                                                                                GCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC                                                                                                                        >  M01186:129:000000000‑A5M8V:1:1107:12595:27841/1‑190 (MQ=60)

                                                                                                                                                                                           GTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGACGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCGGTTTAACCAGTTCGTGAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCCAATTGAGCGAGTCA                                                >  M01186:129:000000000‑A5M8V:1:2103:16151:23793/1‑251 (MQ=60)

                                                                                                                                                                                            tggtccacggctgGTTAACCGAACTGGAAGGCGGCGCGCGC‑CCGCCATGATGG‑CGCAGGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATC                                                 <  M01186:129:000000000‑A5M8V:1:2113:21050:14584/238‑1 (MQ=60)

                                                                                                                                                                                                      gttgtaaaccaaccgGGAAGGCCGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGC                                        <  M01186:129:000000000‑A5M8V:1:1114:14779:18243/235‑1 (MQ=60)

                                                                                                                                                                                                                 AACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAT                                                                                                                                                      <  M01186:129:000000000‑A5M8V:1:2104:28769:15542/129‑1 (MQ=60)

                                                                                                                                                                                                                   CTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGTTAATACCGTTGctgtcacttatccgcatctccgagcccacgagaccgaggttgtcttcgactaccgccatctgcttgataacacagaagtcgttaacct                           >  M01186:129:000000000‑A5M8V:1:1103:17485:20264/1‑162 (MQ=60)

                                                                                                                                                                                                                            CGCGC‑CGAACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACC                 <  M01186:129:000000000‑A5M8V:1:2112:18733:19214/251‑1 (MQ=60)

                                                                                                                                                                                                                             GCGC‑CGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACC                 <  M01186:129:000000000‑A5M8V:1:1108:10001:13961/250‑1 (MQ=60)

                                                                                                                                                                                                                                      CCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGG        >  M01186:129:000000000‑A5M8V:1:2112:7014:16063/1‑251 (MQ=60)

                                                                                                                                                                                                                                       CGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTTATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATCCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTG       <  M01186:129:000000000‑A5M8V:1:2110:10816:4398/251‑1 (MQ=60)

                                                                                                                                                                                                                                            TGATGG‑CGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  <  M01186:129:000000000‑A5M8V:1:1110:6987:4419/251‑1 (MQ=60)

CCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTG‑‑CCACGGCGTGTTAACCGAACTGGAAGGCAGCGC‑CGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTC  >  NC_000913/3560216‑3560698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`