Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F2 I0 R1 145 5829.6 7950970 98.4% 7823754 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,925,289 A→C 12.9% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,925,2890AC12.9% 272.2 / 7.3 148N156T (AAC→ACC) prpC2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (88/37);  new base C (3/20);  total (91/57)
Fisher's exact test for biased strand distribution p-value = 2.97e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428
                                                                                                                                            |                                                                                                                                            
aTGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCTCTTTGTTCGATTCTTCTCTACTGGGTTCAC‑TACAGCCACaa                                                                                                                                              >  5:266033/1‑140 (MQ=255)
aTGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCCCTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACaa                                                                                                                                              >  3:182345/1‑140 (MQ=255)
 tGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGTTTCTTCTCTACTGGTATCAC‑TACAGCCACAAc                                                                                                                                             >  3:497233/1‑140 (MQ=255)
 tGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAAATGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAAc                                                                                                                                             >  4:345748/1‑140 (MQ=255)
   cgcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAAc                                                                                                                                             >  2:176409/1‑138 (MQ=255)
   cgcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACgg                                                                                                                                           <  8:511035/140‑1 (MQ=255)
   cgcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACgg                                                                                                                                           <  7:915758/140‑1 (MQ=255)
    gcACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGc                                                                                                                                          >  5:129011/1‑140 (MQ=255)
     cACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCGACAACGGCg                                                                                                                                         >  8:44092/1‑140 (MQ=255)
      aCCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGa                                                                                                                                        >  4:909167/1‑140 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACCCCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGagc                                                                                                                                      >  8:179619/1‑138 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCAGTTAGTTCGATTCTGCTCTACTGGTATCAC‑TACCGCCACAACGGCGacc                                                                                                                                      >  4:124651/1‑138 (MQ=255)
        cGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACCGCCACAACGGCGAAc                                                                                                                                      >  6:479936/1‑140 (MQ=255)
                 cgcgCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGc                                                                                                                             <  5:899275/140‑1 (MQ=255)
                             cACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTATCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑tgatt                                                                                                                >  3:291974/1‑139 (MQ=255)
                                  tGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGTTATTGCCGACAAACTGCTGGCGTCACTTTCTTCGATTCTTCACTACTGGTATAAC‑TACAGCCACAACGGCGAACTCATCCAGCCGGAAAC‑TGATgacgcc                                                                                                           >  5:539785/1‑138 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGActc                                                                                                         >  4:146998/1‑140 (MQ=255)
                                    ccAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑AACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGCCGActc                                                                                                         >  3:815266/1‑140 (MQ=255)
                                      aGAAAAAGAGGGGCACCCCGTTGCTGGTGCGCGGTATATTGCCCACACACGGCTGGCGTCACTTCGTGCGATTCTTCTCTACTGGTATCCC‑TACCGCCACAACGGCGAACCCATCCCGCCGGAAAC‑TGATGACGActc                                                                                                         <  2:994860/138‑1 (MQ=255)
                                      aGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGGTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGGGAACGCATCCAGACGGAATC‑TGATGACGActg                                                                                                         >  1:941766/1‑137 (MQ=255)
                                              gggggCACACGTTTTCTGGTGCGTGGTATATTGCCGACAAACTGCTGGCGGCACTTAGTTCTATTCTTCTCTAATGTTATAAA‑TACAGCCACAACGGCTAACGCATCCAGCGGTAAAC‑TTATGACGACTCTATcggcg                                                                                                 <  2:34347/137‑1 (MQ=255)
                                              agGGGCACACCGTTGCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGTTATCCC‑TACAGCCACACCGGCGAACGCATCAAGCCGGAAAC‑TTATGACGACTCTATaggaggg                                                                                               >  7:792995/1‑139 (MQ=255)
                                                gggCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCAGCCAGCCGGAACC‑TGATGACGACTCTATCGGCGGTCa                                                                                             >  5:779215/1‑140 (MQ=255)
                                                gcccaccCCGTTCCTGGTGCGCTGGATATTGCCGACAAACTCCTGGCGGCCATACGATCGATACTTCTCTACGGGTATCAC‑ACCAGCCCCAACCGCTAACCCACCCAGCCGTAAAC‑TGATCACGACTCTATCGGCGGTCa                                                                                             <  6:98173/137‑1 (MQ=255)
                                                     cacCGTTGTTGGGGCGCGGGAATTTGCCCACAAACTTGTGGCTTCATTTAGTCCGTTTCTTATAAACTGGTTACAC‑TACCGCCAAAAGGGAGAACCCATCCACCCGGAAAA‑TGATGACGACTCTATCGGCGGTCACTTcc                                                                                        <  8:795813/140‑1 (MQ=255)
                                                        gttttCTGGGGCGCGGTATATTGCCGCAAAACGGCTGGCGTCACTTAGTGCGATTCTTCTCTACTGGTCACAT‑AACAGCCACAACGGCGACCGCACCCCCCCGGAAAC‑TGAGGACGACTCTATCGGCGGTCACTTCCTGc                                                                                     <  4:192060/138‑1 (MQ=255)
                                                        ggTTTCTGGTGGGCGGCATATTGCCTAAAAACTGCTGGCGTCAATTTTTTCGATTATTCTATCCTGGAACCAC‑ACCAGCCACAACGGCTAACGAATCCAGCCGGAAAC‑TGTTGACGACTCTATCGGCGGTCACTTCCTGc                                                                                     <  7:871512/139‑1 (MQ=255)
                                                          tttCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCAt                                                                                   >  5:937999/1‑140 (MQ=255)
                                                             cTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATc                                                                                  >  1:62308/1‑138 (MQ=255)
                                                                gTGCGCGGGATATTGCCGCCAAACTGCTGGCGTCACTTAGTACGATTCATCTCTACTGGTATCAC‑TACAGCCACACCGGCGAACGCATCCAGCCGGAAAC‑TGAAGACGACAGTATCGGCGGTCACTTCCTGCaacagcag                                                                             >  8:102314/1‑133 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTTCa                                                                           >  5:227559/1‑140 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATACAGCCGGAAAC‑TGATGACGACTCTCTCGGAGGTCACTTCCTGCATatgctg                                                                             >  1:422401/1‑138 (MQ=255)
                                                                  gcgcGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATAAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGAGGTCACTTCCTGCATCTTCTGCa                                                                           >  4:748121/1‑140 (MQ=255)
                                                                  gcgcGGGATATTGCCCACAAACTGCGGGCGTCTCTTTTTGCGATTCTTCTCTCCTGGACACAA‑TACCGCCACCCCGGCGACCGCACCCCGCCGAAAAC‑TGTGCACGACTCTATCGGCGGTCACTTCCTGCATctgctg                                                                             <  1:16563/138‑1 (MQ=255)
                                                                    gcgGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACg                                                                         >  5:348682/1‑140 (MQ=255)
                                                                        gATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACCGCGa                                                                     >  7:277639/1‑140 (MQ=255)
                                                                             tccctaaaaaCTGGTGGCGTCAATTAGTTCTATTCTTCTCTACTTGTTTCAC‑TACACCCACAACGGCTAACGCATCCAGCCGTAAAC‑TTCGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGc                                                                <  8:891862/133‑1 (MQ=255)
                                                                             tGCCTACAAACTGCGGGCGTCACTAAGTTCTATTCTTCTCTACTGTTATCAC‑AACCCCCACAACGGCGAACGCATCCCGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGc                                                                <  4:934390/140‑1 (MQ=255)
                                                                             tGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATAAC‑TACAGCCACAACGGCGAACGCAGCCAGCCGGAAAC‑TGATGAAGACTCTATAGGCGTTCACTTACTGCATCTGCTGCACTGCGAAAAGc                                                                >  4:866072/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  8:351998/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  4:840332/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACACTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  7:911393/1‑140 (MQ=255)
                                                                                  aCAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGAGAACGCATCCTGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACATCCTGCATCTGCTGCTCGGCGAAAAGCAGTCg                                                           >  6:373552/1‑140 (MQ=255)
                                                                                    aacctgctgGCGTCACTTAGTGCGATTCTTCTCTACTGGAATCAC‑TACACCCACAACGGCTAACGCACCCCGCCGTAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           <  1:432316/135‑1 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTAGTGGTATCAC‑TCCCCCCACAACGGCGAACGCAGCCAGCCGTAAAC‑TGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCa                                                         <  7:190921/140‑1 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAACGCCGTCg                                                           >  1:109231/1‑138 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  1:719329/1‑138 (MQ=255)
                                                                                    aaaCTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCg                                                           >  2:814031/1‑138 (MQ=255)
                                                                                       ctgctgGCGTCACTTAGTTCGATTCTTCTCAACTGGTA‑CACATACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGCTCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAg                                                      <  7:426120/140‑1 (MQ=255)
                                                                                       ctgctgGCGTAACTTATGTCGATTATAATAAACTGGAAACAC‑TCCAGCCACAACGGCTAACGCAGCCAGCGGTAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAg                                                      <  3:705867/140‑1 (MQ=255)
                                                                                         gctgGCGTCACTTAGTTCGATTCTTCTATACTGGTATCAA‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCTGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGcg                                                    >  4:431856/1‑139 (MQ=255)
                                                                                                 aatttagttagTTTCTTCTCTAGTGGTATCAC‑TACACCCACACCGGCGAACGCATCAAGCCGTAAAC‑TTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAg                                            <  5:136411/137‑1 (MQ=255)
                                                                                                  aCTTATTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCTAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGaaaa                                             <  2:303700/138‑1 (MQ=255)
                                                                                                  aCTTAGTTCTATTGTTCTCTACTGCTTACAA‑TACAGCCACACCGGCGACCGCATCCAGCCGGAAAC‑TCTTGACGACTCTATCGGCGTTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           <  4:828395/140‑1 (MQ=255)
                                                                                                   cTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAg                                            >  1:890455/1‑138 (MQ=255)
                                                                                                    ttcgtgcGATGCTTCTCTACTGGTACAAC‑AACCCCCCCACCGGCGACCGCATCCCCCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCg                                         <  6:406030/134‑1 (MQ=255)
                                                                                                    ttAGTTCGATTCTTCTATACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAgg                                           <  2:962008/138‑1 (MQ=255)
                                                                                                       gtacgtttctacTCTTCTGTTAGAAG‑TACCCCCACACCGGCGAAATCAACCAGCCGTATAA‑GGATGACGACTCTATCGGCGGTCACTTCCTGCATCTTCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGa                                        <  2:672940/132‑1 (MQ=255)
                                                                                                          cGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGTAAAC‑tgat                                                                                                                 <  2:471146/62‑1 (MQ=255)
                                                                                                          cGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑tgat                                                                                                                 >  1:471146/1‑62 (MQ=255)
                                                                                                            attattCTCTACTGTTATCAA‑TACAGCCACAACGGCGAACGCATCCTGCGGCAAAC‑TCATGACGACTCTATCGGCGGGCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  3:746823/140‑1 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTATCCC‑TACACCCACAACGGCGAACGCACCCACCCGGAAAC‑TTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  8:649791/140‑1 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGaaa                                                                                                                       >  5:668171/1‑55 (MQ=255)
                                                                                                            aTTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGaaa                                                                                                                       <  6:668171/55‑1 (MQ=255)
                                                                                                            aTTCTTCTCTACGGGAACCAC‑AACCCCCACAACGGCGAACGCATCCCGCCGTAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  5:599365/140‑1 (MQ=255)
                                                                                                            aTTCTTATCTACTGGAATCAC‑AACCGCCACAACGGCTAACGCATCCCGCCGGAAAC‑TTATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  4:663970/140‑1 (MQ=255)
                                                                                                              tcttctCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGCCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 >  1:701908/1‑138 (MQ=255)
                                                                                                              tcttctCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCAATCGGCGGTCACTTCCAGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 >  1:332510/1‑138 (MQ=255)
                                                                                                                ttctCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCg                             >  8:873534/1‑140 (MQ=255)
                                                                                                                 ccgaaaCTGGTAGCAC‑AACCCCCACACCGGCTAACGGAGCCCGCCGGAACC‑GGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  4:390808/135‑1 (MQ=255)
                                                                                                                  ctctACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGACGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCt                           >  3:132772/1‑140 (MQ=255)
                                                                                                                   actACTGGCATAAA‑TCCAGCCACACCGGCCAAAGCAACC‑TCCGGCATCGTGAGGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            <  1:296840/137‑1 (MQ=255)
                                                                                                                    ctACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCt                           >  1:822513/1‑138 (MQ=255)
                                                                                                                    ctACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCt                           >  1:416223/1‑138 (MQ=255)
                                                                                                                       cTGGTATCAC‑AACACCAACAAGGGCGAACCCACCCAGCCGCAACC‑TGAGCACGACTCTATCGGCGGTCACTACCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGAGc                      <  7:769655/140‑1 (MQ=255)
                                                                                                                          gTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACTGCGAAAAGACGTCGCAAAGCTTGGAAAAGGCGATGCATATCTCTCTGGTGCTGt                   >  4:72473/1‑140 (MQ=255)
                                                                                                                            aTCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGt                   >  2:734211/1‑138 (MQ=255)
                                                                                                                            aTCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTAc                 >  6:7086/1‑140 (MQ=255)
                                                                                                                            aTCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTAc                 >  5:961283/1‑140 (MQ=255)
                                                                                                                             tCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACg                >  6:511262/1‑140 (MQ=255)
                                                                                                                               aC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAACGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  7:632757/1‑140 (MQ=255)
                                                                                                                               aC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  5:558368/1‑140 (MQ=255)
                                                                                                                               aC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  8:236954/1‑140 (MQ=255)
                                                                                                                               aC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGcc              >  7:402124/1‑140 (MQ=255)
                                                                                                                                  tACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGa            >  5:350141/1‑140 (MQ=255)
                                                                                                                                    cAGCCACACCGGGTAACGCACCCAGCGGTAAAG‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTTGTGCTGTACGCCGAac          <  4:404057/140‑1 (MQ=255)
                                                                                                                                     cgctacaACGTCGAACGAACACAGCCGGTAAC‑TGAGTCCGACTCTATCGGCGGTCACGTCCTGCTTCTGCTGCACTGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGAGGCATATCTCGCTGGTGCTGTACGCCGaa           <  2:81980/134‑1 (MQ=255)
                                                                                                                                     cgCCACAACGGCTAACGCATCCACCCGGAAAC‑TGATGACGACTCTTTCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGGCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         <  3:59220/139‑1 (MQ=255)
                                                                                                                                     ccccACACCGGCTACCGCAGCCAGCCGTAAAC‑TGATCACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         <  8:898738/138‑1 (MQ=255)
                                                                                                                                     aGCCACACCTGCGAACGCATCCAGCCGGAAAC‑TGATGACGCCTCTATCGGCGGTCTCTTCCTGCATCTGCTGCACGGCGAAACGCCTTCGCAAAGCTGGGAAAAGGCGATGCATATATGCCTGGTGGTGTACGCCGAacc         >  7:410843/1‑139 (MQ=255)
                                                                                                                                     aGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACtt                                                                                          <  6:894192/59‑1 (MQ=255)
                                                                                                                                     aGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 <  6:82824/116‑1 (MQ=255)
                                                                                                                                     aGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCatat                                 >  5:82824/1‑116 (MQ=255)
                                                                                                                                     aGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGAATATCTCGCTGGTGCTGTACGCCGAaca         >  4:621119/1‑140 (MQ=255)
                                                                                                                                       ccACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAaca         >  2:670130/1‑138 (MQ=255)
                                                                                                                                        cacaACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAacac        >  2:219041/1‑138 (MQ=255)
                                                                                                                                        cacaACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAacac        >  2:768418/1‑138 (MQ=255)
                                                                                                                                            aCGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCCCTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGttt  >  7:830308/1‑140 (MQ=255)
                                                                                                                                            aCGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCTATGCATATCTCGc                            <  3:291368/114‑1 (MQ=255)
                                                                                                                                            aCGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGc                            >  4:291368/1‑114 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATGCGCACCGGCGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCACACCGTTTCTGGTGCGCGGGATATTGCCGACAAACTGCTGGCGTCACTTAGTTCGATTCTTCTCTACTGGTATCAC‑TACAGCCACAACGGCGAACGCATCCAGCCGGAAAC‑TGATGACGACTCTATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCGTCGCAAAGCTGGGAAAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTT  >  NZ_CP022686/3925150‑3925428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A