Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,357,324 A→C 14.9% N37T (AAC→ACC)  dcrB → phage sensitivity protein DcrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,357,3240AC14.9% 259.6 / 18.2 166N37T (AAC→ACC) dcrBphage sensitivity protein DcrB
Reads supporting (aligned to +/- strand):  ref base A (90/47);  new base C (3/25);  total (93/73)
Fisher's exact test for biased strand distribution p-value = 7.22e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

TATGGCCGTTTATCGAAAGGAAGAAGTCAATGCGCAATCTGGTTAAATATGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCCACAGGGCAGAAAG  >  NZ_CP022686/2357186‑2357461
                                                                                                                                          |                                                                                                                                         
         ttATCGAAAGGAAGAAGTCAATGCGCAATCTGGTTAAATATGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCggg                                                                                                                                 >  7:832509/1‑140 (MQ=255)
         ttATAGAAAGGAAGAAGTCAATGCGCAATATGGTTAAATATGTCGGAATTGTCATGCTGGTTATTGGGCTTGCGGCCTGTGATTATAAAGACACTAACGCTATGGAGCAGGGTTCGGTCTCATAAATTAACGCGACCggg                                                                                                                                 >  2:884315/1‑140 (MQ=255)
          tATCGAAAGGAAGAAGTCAATGCGCAATCTGGTTAAATATGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTACCGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGa                                                                                                                                >  8:882385/1‑140 (MQ=255)
                              tGCGCAATCTTGTTAAATATGTCGGATTGGGCCTGCTGGTTAGGGGGCTTGCGGCCGGTGATTATAAAGACACAAACGCTATGGCGCTGGGTTCGGGCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGAt                                                                                                            <  4:219263/140‑1 (MQ=255)
                              tGCGCAATCTGGTTAAATATGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGAt                                                                                                            >  7:610621/1‑140 (MQ=255)
                              tGCGCAATCTGGTTAAATATGTCGGAATTGGCCTGCGGGTTATGGGGCTTGCGGCCTGTGATGATAAACACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGAt                                                                                                            >  7:386041/1‑140 (MQ=255)
                                cgcAATCTGGTTAAATATGTCGTATTGGGCCTGCTGGTTATGGGGTTGGGGGCCTGTTATGATAACGACACTAACGCTATGGCGCCGGGTTCGGGCGCATAAAGTACCGCGACCGGGAATCCCGTCAACCTGCTTGCTgg                                                                                                          <  6:608411/140‑1 (MQ=255)
                                 gaaaTCTGTTTAAATTTGGGGTATTTGGCCGGCTGTTTTGGGGGCTTGGGGCCTGTGATGATAACGACACTACCGCTAGGGCGCTGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGc                                                                                                         <  8:177856/138‑1 (MQ=255)
                                             aaTATGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTtc                                                                                             >  4:451550/1‑140 (MQ=255)
                                              atatGTCGCAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACAATACCGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTtct                                                                                            <  8:150124/140‑1 (MQ=255)
                                               tatGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTtctc                                                                                           >  7:795452/1‑140 (MQ=255)
                                                atGTCGGAAGTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTTATGATAAAGACACTACCGCTATGGCGCAGGGTTCGGCCGCAGAAAGAAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCg                                                                                          <  5:711971/140‑1 (MQ=255)
                                                 tGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGATAGTAACGCGCCCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGc                                                                                         >  7:838621/1‑140 (MQ=255)
                                                 tGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTGGATGGCAAGTGAAGTTTCTCGc                                                                                         >  5:537963/1‑140 (MQ=255)
                                                 tGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTAGGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGc                                                                                         >  1:689042/1‑140 (MQ=255)
                                                   tCGGAATTGGCCTGCTGGTTATGGGGCTTGCGCCCTGTGATGATAAAGACACAAACGCTATGGCGCACGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCAGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTg                                                                                       >  5:827922/1‑140 (MQ=255)
                                                      taaTTGGCCTGCTGGTTTTGGGGCTTGCGGCCGGTCATGATAAAGACACTAACGCTATGGCGCAGGGTGCGGTCGCAGAAAGTACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCa                                                                                    <  2:802931/139‑1 (MQ=255)
                                                      taaTTGGCCTGCTGGTTATGGGGCTGGCGGCCTGTGAGGAAAAACACACTAACGCTATGGCGCAGGGTTCGGCCCCAGAAAGAACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCa                                                                                    <  4:903257/139‑1 (MQ=255)
                                                      taaTTGGCCGGCTGGGTATGGGGCTTGCGGCCTGTTATCAAAAAGACACTCACGCTCTGGCGCCGGGTGCGGGCGCAGAAAGAACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCa                                                                                    <  7:370175/139‑1 (MQ=255)
                                                      taaTTGGCCGGCGGGTTAGGGGGCTTGGGGCCTGTGATTATAAACACACAAACGCTATGGCGCTGGGTGCGGTCGCATAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCa                                                                                    <  1:206806/139‑1 (MQ=255)
                                                      gAATGGGCCGGCTGTTTTGGGGGATTGGGGCCTGTGTTCTTAAGTACACAACCGCTGTGGCGCTGGGTGCGGGCGCACAACTAACCGCGACCGGGAAGCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCa                                                                                    <  1:943106/140‑1 (MQ=255)
                                                      ctcttcgCCTGTGGGTGAGGGGGCGTGCGGTCTGTGTGGAAAATGACACAAACGCTATGGCGCTGGGTGCGGGCGCAGAACGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCa                                                                                    <  1:892486/134‑1 (MQ=255)
                                                      caagtGGCCTGCGGGTTAGGGGGCTAGCGGCCGGTGATGAAAAACACACTAACGCTCTGGCGCAGGGTTCGGGCGCAGAAAGAAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCa                                                                                    <  3:512139/136‑1 (MQ=255)
                                                       aaTTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAg                                                                                   >  8:268858/1‑140 (MQ=255)
                                                        aTTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGc                                                                                  >  8:241822/1‑140 (MQ=255)
                                                        aTTGGCCTGCGGTTTATGGGGCTGGCGGCCTGTTATTATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCACAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGc                                                                                  <  6:107935/140‑1 (MQ=255)
                                                         ttGGCCTGCTGGTTATGGGGCTTTCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAAAGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCg                                                                                 >  6:255142/1‑140 (MQ=255)
                                                         ttGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATTGCAAGTTAAGTTTCTCGCTGCCAGCg                                                                                 >  7:717616/1‑140 (MQ=255)
                                                           ggCGTGCTGTTTTTGGGGCTTGCGCCGGGTTATTAAAAACACACAACCGCTAGGGCGCCGGGTTCGGGCCCACAACGACCCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGa                                                                               <  8:494671/140‑1 (MQ=255)
                                                           ggCCTGGTGGTTATGGGGCTGGCGGCCTGTGATGAAAAAGACACTAACGCTATGTCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGa                                                                               <  2:223259/140‑1 (MQ=255)
                                                           ggCCTGCTGGTTTGGGGGCTGGGGGCGTGTTATTTAAAACCCCCTACCGCTCTGGCGCCGGGTGGGGGCGCAGAACGTACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGa                                                                               <  4:246968/140‑1 (MQ=255)
                                                            gCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACACGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGat                                                                              >  7:811055/1‑140 (MQ=255)
                                                             ccggctgGTTATGGGGCTTGCGGCCTTTTATGATAAAGACACTACCGCTTTGGCGCAGGGTGCGGTCGCATAATGTATCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGata                                                                             <  6:827922/137‑1 (MQ=255)
                                                                 ctgGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATccc                                                                                                                           >  7:413890/1‑90 (MQ=255)
                                                                 ctgGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATccc                                                                                                                           <  8:413890/90‑1 (MQ=255)
                                                                 ctgGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATgac                                                                         >  5:599423/1‑140 (MQ=255)
                                                                       tgggggTTTGCGGCTTTGTATAATAAAGCAACAAAGGCTATGTCGCTTGGTTTGGTCGCAGAAAGTAACGCGACCGGGAATACCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCa                                                                   <  5:450097/138‑1 (MQ=255)
                                                                         ggggCTGGCGCCCTGTTATGATAAACACACTAACGCTATGGCGCAGGGTGCGGTCCCATAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCaga                                                                 <  4:315012/140‑1 (MQ=255)
                                                                           ggCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGc                                                               >  8:870429/1‑140 (MQ=255)
                                                                           ggCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGc                                                               >  5:128918/1‑140 (MQ=255)
                                                                           ggCTGGCGGCCTGTGTTTAAAAAGAACATAACGTTATGGCGGTGGGTTCGGTCGATTAAATTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACGGACCAGAGc                                                               <  4:784084/140‑1 (MQ=255)
                                                                              ttGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCTGCGGATATGACCGACCAGAGCGGt                                                            >  6:566975/1‑140 (MQ=255)
                                                                              ttGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGt                                                            >  7:353508/1‑140 (MQ=255)
                                                                              ttGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGt                                                            >  3:623084/1‑140 (MQ=255)
                                                                              ttGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGt                                                            >  5:708714/1‑140 (MQ=255)
                                                                              ttGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCACGCTGCCAGCGGATATGACCGACCAGAGCGGt                                                            >  4:202277/1‑140 (MQ=255)
                                                                              ttGCGGCCTGTGATGATAAAGACACTAAAGCTATGGCGCAGTGTTCGTTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCTCTGCCAGCGGATATGACCGACCAGAGCgtt                                                            >  4:206697/1‑138 (MQ=255)
                                                                              gggCGGCCGGTTATTATAAACACAATACCGCTATGGCGCTGGGTGCGGGCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGt                                                            <  8:318574/138‑1 (MQ=255)
                                                                               tGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTa                                                           >  2:476922/1‑140 (MQ=255)
                                                                                   gCCGGTGATTATAAACACACTAACGCTATGGCGCAGGGTTCGGGCGCAGAACGAACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCt                                                       <  7:904042/140‑1 (MQ=255)
                                                                                      tgtgATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTggg                                                    >  8:721125/1‑140 (MQ=255)
                                                                                          atgatAAAGACACTACCGCTAGGGCGCAGGGTGCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACg                                                <  8:674212/140‑1 (MQ=255)
                                                                                                aaGACACTACCGCTATGGGGCAGGGTTCGGGCGCATAACTTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGcc                                          <  2:501861/140‑1 (MQ=255)
                                                                                                  gaaacTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGATAAGTTTCTCGCTTCCAGCGGATATGACCGACCAGAGCGCTAAGCTCGGAACGCAGGCCaa                                        >  2:357079/4‑140 (MQ=255)
                                                                                                  cacacacaCGCTAGGGCGCAGGGGTGGGTCGCAGAATCTACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCaa                                        <  7:229898/133‑1 (MQ=255)
                                                                                                    ccaaaaCGCTATGGCTCCGGGGCCGGCCGCATAAAGAACCGCGACCGGGAATCCCGGCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATa                                      <  6:800190/136‑1 (MQ=255)
                                                                                                    cacaAACGCTATGGCGCAGGGTGCGGCCGCACAAAGAACCGCGACCGGGAATCCCGTCAACCTGCTGGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATa                                      <  7:748216/140‑1 (MQ=255)
                                                                                                    cacTACCGCTAGGGCGCAGGTTTCGGGCGAAGAAAGACACACGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATa                                      <  8:811055/140‑1 (MQ=255)
                                                                                                    cacTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATa                                      >  5:532298/1‑140 (MQ=255)
                                                                                                    caataccGCTATGGCGCCGGGTGGGGTCTCATAATGTACCGCGACCGGGATTCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGCCCAGAGCGGTAAGCTGGGAACGCAGGCCAATa                                      <  3:146375/134‑1 (MQ=255)
                                                                                                    aaatAAGGCTTTGGCGCAGGGTTGGGTCCCTCAATGAAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTATGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATa                                      <  6:349238/137‑1 (MQ=255)
                                                                                                       tAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTACCTCGACCGGGAATCCCGTCAACCTGCGTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACGAGAGCGGTAAGCTGGGAACGCAGGCCGATAAca                                   >  5:547655/1‑140 (MQ=255)
                                                                                                       tAACGCTATGGCGCAGGGTTCGGTAGCAGAAAGTACCGCGACCGGGACTCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGTGAACGCCGGCCAATAAca                                   >  4:257477/1‑140 (MQ=255)
                                                                                                         ccGCTATGGCGCAGGGTGCGGGCGCAGAAAGTACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAAcatg                                 <  2:615162/139‑1 (MQ=255)
                                                                                                         aCGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGTCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGCCCAGAGCGGTAAGCTGGGAACGCAGGCCAATAAcatg                                 >  1:353603/1‑140 (MQ=255)
                                                                                                         aCGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAAcatg                                 >  2:175693/1‑140 (MQ=255)
                                                                                                         aCGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAAcatg                                 >  2:84949/1‑140 (MQ=255)
                                                                                                          ctcTATGGCGCTGTGGTCTGGTGCATAACGTAACGAGACCGGGAAGCCCGTCCACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAAcatgc                                <  7:715669/138‑1 (MQ=255)
                                                                                                          cGCTATGCCGCCGGGGTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAAcatgc                                <  4:450346/140‑1 (MQ=255)
                                                                                                            cTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGTCCAGAGCGGTAAGCTGGGACCGCAGGCCAATAAcatgcat                              >  7:291850/1‑140 (MQ=255)
                                                                                                               tGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGCCCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTc                           >  2:321955/1‑140 (MQ=255)
                                                                                                               tGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCaa                                        <  6:128388/127‑1 (MQ=255)
                                                                                                               tGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCaa                                        >  5:128388/1‑127 (MQ=255)
                                                                                                               tGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTc                           >  4:659085/1‑140 (MQ=255)
                                                                                                               tGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTAAAGTTTCTCGCTGACAGCGGATATGACCGACCAGAGCGGTAAGCTGTGATCGCAGGCCAATAACATGCATGTc                           >  8:901896/1‑140 (MQ=255)
                                                                                                                ggCGCCGGGGGCGGGCGCAGAAAGTACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCt                          <  1:674378/140‑1 (MQ=255)
                                                                                                                  cgcAGGGTGCGGTCTCAGAAAGAAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTATCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTgg                        <  4:143572/140‑1 (MQ=255)
                                                                                                                    gtgggTTTGGCGGCAAAAATTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTc                      <  4:779540/138‑1 (MQ=255)
                                                                                                                    cAGGGTTCGGTCGCTGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGCGCGGTAAGCTGGGAGCGCAGGCCAATAACATGCATGTCTGGTc                      >  2:867880/1‑140 (MQ=255)
                                                                                                                    cAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTc                      >  7:780789/1‑140 (MQ=255)
                                                                                                                     aGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTcc                     >  7:541310/1‑140 (MQ=255)
                                                                                                                     aGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTcc                     >  6:113561/1‑140 (MQ=255)
                                                                                                                       ggTTCGTTCGCATAAAGTAACGCGACCGGGAATCCCGGCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGa                   <  5:294347/140‑1 (MQ=255)
                                                                                                                       ggTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTACGa                   >  2:851837/1‑140 (MQ=255)
                                                                                                                       ggTTCGGCCGCATAAAGAAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGa                   <  1:871163/140‑1 (MQ=255)
                                                                                                                       ggTGCGGGCGCACAAAGAACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGa                   <  5:592132/140‑1 (MQ=255)
                                                                                                                         ttCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGACCGCAGGCCAATAACATGCATGTCTGGTCCGACg                 >  8:290396/1‑140 (MQ=255)
                                                                                                                         ttCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGAGGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACg                 <  5:539512/140‑1 (MQ=255)
                                                                                                                            ggccGCATAAAGAAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCca              <  1:498763/137‑1 (MQ=255)
                                                                                                                            ggTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGCCCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGccc              >  5:324035/1‑139 (MQ=255)
                                                                                                                            ggTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCgccgccc              >  6:869546/1‑139 (MQ=255)
                                                                                                                            ggTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCca              <  6:599423/140‑1 (MQ=255)
                                                                                                                            ggTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCca              >  3:151984/1‑140 (MQ=255)
                                                                                                                              gcccataaaGAAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCcaca            <  2:707502/134‑1 (MQ=255)
                                                                                                                              gcGCAGAAATTAACGCGACCGGGAATCACGTCAACCTGCTTGATGGCAAGATAAGTTTCTCTCTGCCTGCGTATATGACAGACCATATCGGTAAGCTGTGAACGCATGCCAATAACATGCATGTCTGGTCCGACGCcaca            <  8:538786/139‑1 (MQ=255)
                                                                                                                              gcGCAAAAATAAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGTATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCAAATAACATGCATGGCTGGTCCGACGCcaca            <  7:172226/139‑1 (MQ=255)
                                                                                                                              ccGCATAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCcaca            <  1:128298/139‑1 (MQ=255)
                                                                                                                              ccGCAGAAAGTACCGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCGCGCTGCCAGCGGATATGACCGCCCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCcaca            <  1:738733/139‑1 (MQ=255)
                                                                                                                                   taaaGTAACGCGACCGGGAATCCCGTCAACCTGGTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTTGTCCGACGCCACAGGGCa       <  6:685193/139‑1 (MQ=255)
                                                                                                                                      attAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCCACAGGGCAGaa    <  3:761946/138‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TATGGCCGTTTATCGAAAGGAAGAAGTCAATGCGCAATCTGGTTAAATATGTCGGAATTGGCCTGCTGGTTATGGGGCTTGCGGCCTGTGATGATAAAGACACTAACGCTATGGCGCAGGGTTCGGTCGCAGAAAGTAACGCGACCGGGAATCCCGTCAACCTGCTTGATGGCAAGTTAAGTTTCTCGCTGCCAGCGGATATGACCGACCAGAGCGGTAAGCTGGGAACGCAGGCCAATAACATGCATGTCTGGTCCGACGCCACAGGGCAGAAAG  >  NZ_CP022686/2357186‑2357461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A