Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F13 I0 R1	150	9496.0	7621532	98.4%	7499587	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	3,604,564	G→T	13.6%	intergenic (+80/‑151)	lpxC → / → secM	UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	3,604,564	0	G	T	13.6%	174.5 / 5.4	76	intergenic (+80/‑151)	lpxC/secM	UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
Reads supporting (aligned to +/- strand): ref base G (5/59); new base T (10/2); total (15/61)
Fisher's exact test for biased strand distribution p-value = 1.80e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATCGG  >  NZ_CP022686/3604425‑3604702

ttCCAGGACGACGCAGAACTGCCGGTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCCGTCG‑T‑‑TTTTTTTTg                                                                                                                                            <  4:700181/140‑1 (MQ=255)

 tCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTgt                                                                                                                                           >  8:703521/1‑139 (MQ=255)

  ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGCACCAGTCG‑T‑‑GGTTTTTTGat                                                                                                                                          >  7:610225/1‑139 (MQ=255)

  ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGaa                                                                                                                                          <  8:302309/140‑1 (MQ=255)

        cgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTtat                                                                                                                                    <  2:280740/140‑1 (MQ=255)

         gacgAAGTACTGCCGTTGGCCTTCAAAGCGCCTTCAGCAGTACTGGCATAACGACATTTTTACTGTCGTATTAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTtatt                                                                                                                                   <  2:397135/140‑2 (MQ=255)

          acgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATaa                                                                                                                                  <  2:761420/140‑1 (MQ=255)

           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑Ggttttttgttttttttttt                                                                                                                                 >  3:169475/1‑135 (MQ=255)

           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GTTTTTTTTTTTTTTATaat                                                                                                                                 >  2:937746/1‑139 (MQ=255)

           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GTTTTTTTTTAtttttttaa                                                                                                                                 >  5:598529/1‑135 (MQ=255)

           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GTTTTTTTTTATTTTTTaat                                                                                                                                 >  2:450205/1‑139 (MQ=255)

           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GTTTTTTTGGAtttttttaa                                                                                                                                 >  2:842868/1‑135 (MQ=255)

           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GGTTTTTTGGTTTTtatttt                                                                                                                                 >  5:661806/1‑137 (MQ=255)

           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAAAGACATTTATACTGTCGTATAAATTTCTACTCGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TGTGTTTTTCATTTTATaat                                                                                                                                 >  2:380857/1‑139 (MQ=255)

           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCAAAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGCACCAGTCT‑C‑‑GTTTTTTTGTATTTTTTaat                                                                                                                                 >  8:158291/1‑139 (MQ=255)

             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAagag                                                                                                                               <  6:506247/140‑1 (MQ=255)

             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAagag                                                                                                                               <  5:291333/140‑1 (MQ=255)

             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAagag                                                                                                                               <  8:57326/140‑1 (MQ=255)

             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAagag                                                                                                                               <  2:963702/140‑1 (MQ=255)

             cAGAACTGCCGTTGGCCTTCAAAGCGCCATCAGGCGTACTGGCATAACGAAATTTAAACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCATTCG‑T‑‑TTATTTTTGAATTTTATAagag                                                                                                                               <  7:329209/140‑1 (MQ=255)

                     ccGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GTTTTTTTTATTTTTTTAATAGCTATaaaa                                                                                                                       >  2:726131/1‑140 (MQ=255)

                     ccGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GTTTTTTTTAATTTTTTAAAAG‑AATaaaaa                                                                                                                      >  4:730785/1‑140 (MQ=255)

                        actgCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATTCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAAGCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                                                                                    <  7:17823/137‑1 (MQ=255)

                         tGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTAGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTT‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                                                                                    <  7:771413/140‑1 (MQ=255)

                          ggCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTTG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                                                                                  >  5:816193/1‑140 (MQ=255)

                          ggCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GGTTTTTTTATTTTTATAAGAGGGATaaaaaaacc                                                                                                                  >  2:214442/1‑137 (MQ=255)

                               tCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGa                                                                                                             <  4:346322/140‑1 (MQ=255)

                                  aaGCGCCTGCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                                                                          <  5:506683/140‑1 (MQ=255)

                                  aaGCCCCTTCAGCCGTACTGGCATAACGACAATTATACTCTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                                                                          <  1:657181/140‑1 (MQ=255)

                                       ccTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCCCTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                                                                     <  5:519288/140‑1 (MQ=255)

                                       ccTTCAGACGTACTGGCATAAAGACATTTATACTGTCGTATAAAATTCGCATGGCAAATCTGGCACTCTCTCCGTCCAGGTGAACAAGTCG‑TGATTTTTTTTTAATTTT‑T‑ATATCTATAAAAAAAGATGGGAACgggatt                                                                                                     >  1:98383/1‑135 (MQ=255)

                                            aGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                                <  5:429201/140‑1 (MQ=255)

                                               cGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                             <  7:818750/140‑1 (MQ=255)

                                               cGTACTGCCATAACGACATTTATACTTTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGGCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                             <  7:356052/140‑1 (MQ=255)

                                                 tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GTTTTTTTTTATTTTTTAAGAGCGATAAAAAAAGGTTGGCACCCTGTTTTGtttatca                                                                                           >  8:586267/1‑135 (MQ=255)

                                                    tGGCATAACGAGCTTTATACTGTCGTTTAAAATTCGACTGGCAAATCTGGTACTGTCTGCGGCCTTGTGAAACAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAATAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                                                                        <  4:487735/140‑1 (MQ=255)

                                                      gCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                                                                      <  7:373001/140‑1 (MQ=255)

                                                      gCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑GTTTTTTTTTATTTTTTTTATGGTATAAAAAAAAGTGGGGGCGCGGTTTTTTTAAGCACtttt                                                                                      >  1:514445/1‑140 (MQ=255)

                                                       cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTa                                                                                     <  8:220248/140‑1 (MQ=255)

                                                        aTAACGCAATTTATCCTGTCGAATATAATTCTCCTCGAAATAGTGGAACTCTATCCTGCCCGGTTAACCAGTC‑‑T‑‑TTTTTTTTTTATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                   <  6:559598/140‑1 (MQ=255)

                                                        aTAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAAGCTCGCACTCTCTCCGGCCAGGTGAACCAGTCG‑‑‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAACACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                   <  1:88560/140‑1 (MQ=255)

                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCTGCCAGGTGAACCAGTAG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                   <  4:294177/140‑1 (MQ=255)

                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                   <  3:777620/140‑1 (MQ=255)

                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                   <  2:506686/140‑1 (MQ=255)

                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                   <  7:423220/140‑1 (MQ=255)

                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCCGGCACTCTCTCCGGCCAGGTGAACCAGGCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                   <  5:753201/140‑1 (MQ=255)

                                                               cATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAAc                                                                             <  7:293028/140‑1 (MQ=255)

                                                                aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACt                                                                            <  2:33330/140‑1 (MQ=255)

                                                                         ggcGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCAt                                                                   <  2:617058/138‑1 (MQ=255)

                                                                           gttataAAATTCTACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTc                                                                 <  3:49912/138‑1 (MQ=255)

                                                                              ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTg                                                              <  1:627766/140‑1 (MQ=255)

                                                                              ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTg                                                              <  4:244273/140‑1 (MQ=255)

                                                                                   aTTCGACTGGCATATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCtgtg                                                         <  6:209747/140‑1 (MQ=255)

                                                                                   aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCtgtg                                                         <  6:44377/140‑1 (MQ=255)

                                                                                   aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCtgtg                                                         <  4:17167/140‑1 (MQ=255)

                                                                                   aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCtgtg                                                         <  1:73382/140‑1 (MQ=255)

                                                                                        ctaggCAAATCTGGCACGCTCTCCGGCAAGGTGAACCATTCG‑T‑‑TTTTTTTTTAATTTTTTAAGAGATATAAAAAACGTGGCGAACGCTGTTTTCTTAAGCACTTTTACTCACAACTTATCTTCATTCGTGCTGTGGACtt                                                    <  5:917254/137‑1 (MQ=255)

                                                                                         cTGGCACAACTGGCACTCTCTCCGTGCAGGTGAAACAGTCG‑T‑‑TGTTTTTTGAATTATATAAGAGCTATAAAACACGGTGCGAACTCTGTTTTCTTAAGCTCTTTTACTCACAACTTATCTTCATTCGTGCTGTGGACTTc                                                   <  2:611413/140‑1 (MQ=255)

                                                                                               aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCtt                                             <  7:796811/140‑1 (MQ=255)

                                                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTa                                           <  2:625912/140‑1 (MQ=255)

                                                                                                   tGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAAt                                         <  3:58666/140‑1 (MQ=255)

                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                      <  7:185682/140‑1 (MQ=255)

                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                      <  1:559521/140‑1 (MQ=255)

                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                      <  6:731586/140‑1 (MQ=255)

                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                      <  5:342452/140‑1 (MQ=255)

                                                                                                        ctctctCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATaa                                    <  7:196334/140‑1 (MQ=255)

                                                                                                                ggCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTtgtg                            <  4:628284/140‑1 (MQ=255)

                                                                                                                ggCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTtgtg                            <  3:539794/140‑1 (MQ=255)

                                                                                                                  ccAGGTTAACCTGTCG‑T‑‑TTTTTTTTGAATTTTATAATATCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTgcg                          <  2:240543/140‑1 (MQ=255)

                                                                                                                  ccAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTgcg                          <  6:527552/140‑1 (MQ=255)

                                                                                                                  ccAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTgcg                          <  3:558893/140‑1 (MQ=255)

                                                                                                                               tCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTg             <  5:616376/140‑1 (MQ=255)

                                                                                                                                 g‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa           <  1:849537/140‑1 (MQ=255)

                                                                                                                                 g‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa           <  8:798723/140‑1 (MQ=255)

                                                                                                                                      ttttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATa         <  7:703521/140‑1 (MQ=255)

                                                                                                                                          ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGAt     <  7:671867/140‑1 (MQ=255)

                                                                                                                                           tttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATc    <  7:586267/140‑1 (MQ=255)

                                                                                                                                             tGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATCgg  <  6:180653/140‑1 (MQ=255)

                                                                                                                                             tGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATCgg  <  1:42128/140‑1 (MQ=255)

TTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATCGG  >  NZ_CP022686/3604425‑3604702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A