Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,604,564 G→T 16.9% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,604,5640GT16.9% 237.5 / 16.5 112intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
Reads supporting (aligned to +/- strand):  ref base G (7/84);  new base T (19/2);  total (26/86)
Fisher's exact test for biased strand distribution p-value = 8.48e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATC  >  NZ_CP022686/3604425‑3604700
                                                                                                                                             |                                                                                                                                         
ttCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTGCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTg                                                                                                                                           <  8:788376/140‑1 (MQ=255)
  ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑aa                                                                                                                                        <  2:637250/140‑1 (MQ=255)
  ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑aa                                                                                                                                        <  1:532720/140‑1 (MQ=255)
  ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTgta                                                                                                                                         >  5:957031/1‑138 (MQ=255)
        cgacgCAGAACTGCCGTTGGCCTTCTAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTtat                                                                                                                                  <  7:474557/140‑1 (MQ=255)
        cgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGGCG‑T‑TTTTTTTTG‑AATTTtat                                                                                                                                  <  6:72364/140‑1 (MQ=255)
          acgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCTTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCAGGCCAGGTGACACAGTCG‑G‑AATTTTTTT‑ATTTTTaaaa                                                                                                                                >  8:777339/1‑140 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTtt‑tattttattat                                                                                                                               >  2:948526/1‑137 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTG‑AGTTTTATAag                                                                                                                               >  1:743712/1‑140 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTGT‑TATTTTTTaat                                                                                                                               >  1:716982/1‑139 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTATCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTT‑TATTTTATaaa                                                                                                                               >  6:687923/1‑139 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGAATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGACG‑T‑TTTTTTTTT‑TAtattattaa                                                                                                                               >  7:1003164/1‑137 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCAGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTAG‑T‑TTGTTTTTGTAAtttttttt                                                                                                                                >  7:375268/1‑136 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGCCAATTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAagag                                                                                                                             <  6:114436/140‑1 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTTGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAagag                                                                                                                             <  2:963979/140‑1 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCAGTCTCTACGGCCAGGTGAACCAGTCG‑T‑GGTTTTTTT‑TAATTTATaaa                                                                                                                               >  4:894980/1‑137 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAagag                                                                                                                             <  2:1177738/140‑1 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTT‑TATTTTTTaaa                                                                                                                               >  4:1216952/1‑137 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTT‑AATTTtttatt                                                                                                                               >  4:5068/1‑136 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTAAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAag                                                                                                                               <  4:936784/138‑1 (MQ=255)
             aaGAACTGCCGTTGGCCTTCTAAGCGCCTTCACACGTACTGGCATAACGACATTTATACTGTCGAATAATATTGGACTGTCAAATCTGGCAATCTCTCCGGCCAGGTGTACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAagag                                                                                                                             <  1:187209/139‑1 (MQ=255)
                     ccGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTTT‑ATTTTTATAA‑ATCTATaaaa                                                                                                                     >  7:324708/1‑140 (MQ=255)
                     ccGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GGTTTTTTT‑TATTTTTTTAGAGATATaaaa                                                                                                                     >  5:1229594/1‑140 (MQ=255)
                      cGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGGCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATaaaaa                                                                                                                    <  6:218849/140‑1 (MQ=255)
                        ttGGCCTTCACAGCGCATTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAAc                                                                                                                  <  2:858842/140‑1 (MQ=255)
                        gtGGCCTTCATAGCGCCTTCAGCCGTTCTGTCATAACGACATTTATACTGGCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATaaaaa                                                                                                                    <  4:1102518/137‑1 (MQ=255)
                        gtGGCCTGAAATGCGCCTTGAGCCGTACTGGCATAACGACATATATACTGGAGTATAATCTTCGACTGGCTAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATaaaaa                                                                                                                    <  4:115319/137‑1 (MQ=255)
                          ggCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGCACCAGTCG‑T‑GTTTGTTTG‑TTATTTTTAATAGAAATaaaaaaacg                                                                                                                >  2:1063791/1‑137 (MQ=255)
                          ggCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTTT‑ATTTTTTTATATGTTATaaaaaagg                                                                                                                 >  5:471869/1‑138 (MQ=255)
                          ggCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTT‑TATTTTATAATAACTaaaaaaaaaag                                                                                                                >  8:197057/1‑137 (MQ=255)
                          ggCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTT‑TATTTTTTTATAGGTTTaaaaaaaag                                                                                                                >  6:25324/1‑137 (MQ=255)
                            ccTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTG‑TATTTTTTTAGAGAGATATAAAAccg                                                                                                                >  3:613267/1‑136 (MQ=255)
                               tCAACGCGCCTTCAGCCGTACTGGCATCACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGa                                                                                                           <  5:178643/140‑1 (MQ=255)
                                  actcgcCTTCAGCCGTACTGGCATAACCAAATTTATACTGTCGTATAAAATTCGACTGTCAAATCTGTCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                                                                        <  5:295522/137‑1 (MQ=255)
                                  acgcgcCTTCAGCCGTCCTGGCATAACGACATTTATACTGTCGTATAAAATTCGCCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                                                                        <  2:190534/138‑1 (MQ=255)
                                  aaGCGCCTTCAGCCGTACTTGGATAACGACAGTTATCCTGTCTTATAAAATTCGACTGGCAACTCTGGCAATCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                                                                          <  4:311211/138‑1 (MQ=255)
                                       ccTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGTCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                                                                     <  3:1142188/138‑1 (MQ=255)
                                         ttCAGCCGTTCTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GGTTTTTTT‑TTTTTTTTTATTTGTATAAAAAACCGTGGGGACGcgggt                                                                                                   >  4:194830/1‑134 (MQ=255)
                                          tCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                                                                                <  6:985380/140‑1 (MQ=255)
                                          tCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                                                                                <  1:782380/140‑1 (MQ=255)
                                               cGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                           <  2:66513/140‑1 (MQ=255)
                                               cGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGGGCGAACGCTGTTTTCTTAAg                                                                                           <  1:1301567/140‑1 (MQ=255)
                                               cGTACTGGCAAAACCACATTTATACTGTCGTATAAAAATCGAATGTCACCACTGCCACTCTCTCCGGCCACGTGAACCAGTCG‑T‑TTTTTTTTT‑AATTTTATAAGCTCAATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                           <  6:340799/140‑1 (MQ=255)
                                               cGTAATGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGACAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                           <  2:451365/140‑1 (MQ=255)
                                                 tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCa                                                                                         <  6:386481/140‑1 (MQ=255)
                                                   cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTT‑TTTTTTTTAATATCAATAAAAAAAAGGGAGAACCCGGTTTTTTTaataa                                                                                         >  3:764102/1‑135 (MQ=255)
                                                    tGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                                                                      <  1:481856/140‑1 (MQ=255)
                                                    tGGCATAACGAAATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                                                                        <  4:865466/138‑1 (MQ=255)
                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  5:753197/140‑1 (MQ=255)
                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  1:425234/140‑1 (MQ=255)
                                                         aaaCGACATTTATACTGGCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTa                                                                                   <  3:415711/137‑1 (MQ=255)
                                                          aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTAc                                                                                  <  3:113692/138‑1 (MQ=255)
                                                           aCGACATTTATCCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGca                                                                               <  7:1067872/140‑1 (MQ=255)
                                                           aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  3:278338/138‑1 (MQ=255)
                                                           aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGca                                                                               <  5:1272635/140‑1 (MQ=255)
                                                             gACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTT‑TATTTTATAATATAGATAAAAAACGGTGGGAACGCGGTGTTGTTTAGGTCTTTTACGca                                                                               >  4:617067/1‑138 (MQ=255)
                                                               aaTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCATGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAAc                                                                           <  6:1120620/139‑1 (MQ=255)
                                                               aaTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGcaca                                                                             <  3:1216952/137‑1 (MQ=255)
                                                                 tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACtt                                                                         <  8:118299/140‑1 (MQ=255)
                                                                         ggagTAAAAAAATCGTCTGGCCAATCTGGCACTCTCTCCTGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑ACTTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCAt                                                                 <  1:114532/137‑1 (MQ=255)
                                                                          tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTG‑TATTTTTTTAGGGCTATTTAAAAAGGGGGGGGCGGGGTTTTGTTAAGCAAGTTTAAGCACACCTTATGTTGAtt                                                                >  8:744313/1‑140 (MQ=255)
                                                                          tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTG‑TATTTTTTAATATGGATAAAAAAAGGTGCGAACGCGGGTTTGTTATGGACGTTTACGGACCACTTTTGTTGAtt                                                                >  8:91472/1‑140 (MQ=255)
                                                                           cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGACCCAGTCG‑T‑GTTTTTTTG‑TATTTTTTATGTTCGATTAAAAAAGGGGCGGGCGCGGGTTTGTTTAGGCCATTTTCGCCCCACTTATGttgca                                                                 >  3:1207718/1‑135 (MQ=255)
                                                                           cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCAt                                                                 <  3:1353773/138‑1 (MQ=255)
                                                                            gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCAtt                                                                <  4:881903/138‑1 (MQ=255)
                                                                              ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTg                                                            <  7:563466/140‑1 (MQ=255)
                                                                              ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTg                                                            <  5:395768/140‑1 (MQ=255)
                                                                                aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCt                                                          <  8:57608/140‑1 (MQ=255)
                                                                                 aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGc                                                           <  4:607461/138‑1 (MQ=255)
                                                                                 aaaTTCGACTGGCAAATCTGGCACGCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCtg                                                         <  8:1128241/140‑1 (MQ=255)
                                                                                  aaTTCGACTGGCAAATCTGGCACTCTCTCCTGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGATGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCATCATTCGTGCtgt                                                        <  1:849748/140‑1 (MQ=255)
                                                                                    ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTgg                                                      <  7:1103583/140‑1 (MQ=255)
                                                                                        aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACtt                                                  <  2:66045/140‑1 (MQ=255)
                                                                                        aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTAATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGAc                                                    <  3:270536/138‑1 (MQ=255)
                                                                                         cTGTCAAATCTGGCACTCTCTGCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAATTGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACt                                                   <  4:1197295/138‑1 (MQ=255)
                                                                                               aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCtt                                           <  7:588746/140‑1 (MQ=255)
                                                                                               aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCtt                                           <  1:171105/140‑1 (MQ=255)
                                                                                               aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCtt                                           <  1:1148659/140‑1 (MQ=255)
                                                                                                aTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTT‑TTTTTTTTG‑AATTTTATAAGATCTATAAAACACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCtt                                           <  1:1289563/140‑1 (MQ=255)
                                                                                                aTCTGGCACTCTCTCCGGCCAGGTGAAACAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCttt                                          <  7:827286/140‑1 (MQ=255)
                                                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTa                                         <  6:448118/140‑1 (MQ=255)
                                                                                                 tCTGGCACTCTCTCCGGCCAGGTGAAACAGTAG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTa                                         <  7:172705/140‑1 (MQ=255)
                                                                                                   tGGCACTCTCTCCGGCCAGGTTTACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATATAAAACGGTGCGAACGCGGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAAt                                       <  5:63913/140‑1 (MQ=255)
                                                                                                      caatctctcCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATATAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                    <  5:610978/137‑1 (MQ=255)
                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACTCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                    <  2:801590/140‑1 (MQ=255)
                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                    <  5:942177/140‑1 (MQ=255)
                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                    <  1:1193859/140‑1 (MQ=255)
                                                                                                        ctctctCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATaa                                  <  6:365928/140‑1 (MQ=255)
                                                                                                              ccGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTtg                            <  5:368707/140‑1 (MQ=255)
                                                                                                                ggCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAACCGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTtgtg                          <  7:819014/140‑1 (MQ=255)
                                                                                                                ggCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTtg                            <  3:1130632/138‑1 (MQ=255)
                                                                                                                ggCCAGGTGAACCAGGCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTtg                            <  3:4210/138‑1 (MQ=255)
                                                                                                                  ccAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTtgtg                          <  4:101615/138‑1 (MQ=255)
                                                                                                                  ccAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTgcg                        <  2:614339/140‑1 (MQ=255)
                                                                                                                       tGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTaaa                   <  8:684366/140‑1 (MQ=255)
                                                                                                                            cAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGt              <  5:860214/140‑1 (MQ=255)
                                                                                                                            cAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGGTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATAc                <  3:434458/138‑1 (MQ=255)
                                                                                                                                 g‑T‑TTTTTTTTG‑TATTTTATAAGAGCTATAAAATACTGTGCGAACGCTGTTTTCTTAAGCAATTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGat         <  7:958583/140‑2 (MQ=255)
                                                                                                                                 g‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa         <  7:764612/140‑1 (MQ=255)
                                                                                                                                          tttG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATc  <  1:59370/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                         
TTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATC  >  NZ_CP022686/3604425‑3604700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A