Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,604,564 G→T 10.0% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,604,5640GT10.0% 279.3 / 5.5 120intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
Reads supporting (aligned to +/- strand):  ref base G (12/92);  new base T (13/2);  total (25/95)
Fisher's exact test for biased strand distribution p-value = 5.59e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATC  >  NZ_CP022686/3604425‑3604700
                                                                                                                                            |                                                                                                                                         
ttCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTg                                                                                                                                           <  8:790403/140‑1 (MQ=255)
  ccAGGACGACGCAGAACGGCCGTTGGCCTTCACAGCGCCTTCAGCCGGACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑aa                                                                                                                                        <  6:1178928/140‑1 (MQ=255)
   cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑a                                                                                                                                         <  1:311761/138‑1 (MQ=255)
   cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AAt                                                                                                                                       <  4:1103785/140‑1 (MQ=255)
   cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTT‑AAt                                                                                                                                       >  7:647207/1‑140 (MQ=255)
   cAGGACGACGCAGAAATTCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGAATAAATACATTTATACTGTCTTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AAt                                                                                                                                       <  7:573425/140‑1 (MQ=255)
        cgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AAtttt                                                                                                                                    <  1:1223254/138‑1 (MQ=255)
        cgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTtat                                                                                                                                  <  7:1137613/140‑1 (MQ=255)
        cgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTtat                                                                                                                                  <  4:307497/140‑1 (MQ=255)
         gacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTtata                                                                                                                                 <  3:576891/140‑1 (MQ=255)
          acgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTG‑AAttttttta                                                                                                                                >  6:707208/1‑136 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGCACCAGTCG‑TGGTTTTTTT‑TATTTTTTaat                                                                                                                               >  5:829410/1‑139 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTT‑TTTTTTATaat                                                                                                                               >  7:716691/1‑139 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTGTAATTTtttat                                                                                                                                >  4:960841/1‑139 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTG‑TATTTTTTaat                                                                                                                               >  3:55859/1‑139 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGGTTTTTTG‑TATTTtattat                                                                                                                               >  5:535195/1‑137 (MQ=255)
           cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑GGGTTTTTTT‑TAtttttttaa                                                                                                                               >  5:680238/1‑135 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAagag                                                                                                                             <  4:465292/140‑1 (MQ=255)
             cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAagag                                                                                                                             <  6:233244/140‑1 (MQ=255)
             cAGAACTGCCGTTGGACTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAag                                                                                                                               <  2:517636/138‑1 (MQ=255)
                 aCTGCCGTTGGCCTTCAAAGCGCCTTCAGCCTTACTGGGATAACGACATTTATACTGTCGTATATAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTTAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCt                                                                                                                           <  1:573599/138‑1 (MQ=255)
                     ccGTTGGCCTTCAACGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTAGACTGGCAAATCTTGAACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTG‑AATATTTAAAGAGATATaaaa                                                                                                                     >  6:697689/1‑140 (MQ=255)
                        ttGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAAc                                                                                                                  <  3:91757/140‑1 (MQ=255)
                        ttGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAAc                                                                                                                  <  6:656347/140‑1 (MQ=255)
                          ggCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGCACCAGTCG‑TTTTTTTTTT‑AATTTTTTAATTAATATAAAAAActt                                                                                                                >  6:515894/1‑138 (MQ=255)
                          ggCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTT‑AAATTTTTTAAAACTATaaaaaaacc                                                                                                                >  5:1076531/1‑137 (MQ=255)
                                cAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCg                                                                                                            <  1:1225474/138‑1 (MQ=255)
                                cAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                                                                          <  6:4811/140‑1 (MQ=255)
                                cAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGGCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                                                                          <  3:426505/140‑1 (MQ=255)
                                 aaaGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                                                                          <  8:617197/140‑1 (MQ=255)
                                  aaGCTCCTTCAGCCGTAATGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                                                                        <  4:95633/140‑1 (MQ=255)
                                     cgcCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGc                                                                                                       <  2:320883/138‑1 (MQ=255)
                                            aGCCGTACTTGCATAACGACATTTATACTGGCGTATAAAATTCGACTGGCAAAGCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                              <  3:738839/140‑1 (MQ=255)
                                               cGTTCTGGCATAACGAAATTTATACTGTCGTATAAAATTCGACTGGCAAATATGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                           <  7:74393/140‑1 (MQ=255)
                                               cGTACTGGCATCACGACCTGTATACTGTCGTATAAAATTCGACAGGCAAATCTGGCACTCGCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACTGTGCGAACGCTGTTTTCTTAAg                                                                                           <  3:72402/140‑1 (MQ=255)
                                               cGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                                                                             <  1:11782/138‑1 (MQ=255)
                                               cGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                           <  6:137465/140‑1 (MQ=255)
                                                 tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTT‑TATTTTTTAAGATCGATAAAAAAACGTGGGGACCCTGGTTTATTaatcc                                                                                         >  8:470742/1‑137 (MQ=255)
                                                    tGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                                                                        <  1:65307/138‑1 (MQ=255)
                                                    tGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                                                                      <  7:744065/140‑1 (MQ=255)
                                                     ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                                                                        <  1:194019/138‑1 (MQ=255)
                                                     ggCATAAAGAAATTTTTACTGTTGTATTAATTTCGTCTGGCTAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                                                                        <  1:763698/138‑1 (MQ=255)
                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  8:236721/140‑1 (MQ=255)
                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  4:966095/140‑1 (MQ=255)
                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  3:1190706/140‑1 (MQ=255)
                                                         tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCAGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  3:338760/140‑1 (MQ=255)
                                                         tAAAGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  3:1184685/140‑1 (MQ=255)
                                                         aaaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACg                                                                                 <  5:194586/139‑1 (MQ=255)
                                                          aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGGTTTTTTT‑TATTTTTTAATTGCAATAAAAAACGGTGCGGACGCTGGTTTTTTTTGGACttttta                                                                                  >  2:465058/1‑136 (MQ=255)
                                                           aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGca                                                                               <  3:553306/140‑1 (MQ=255)
                                                            cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTTGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGTACGCTGTTTTCTTAAGCACTTTTACGcac                                                                              <  7:517828/140‑1 (MQ=255)
                                                            cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGcac                                                                              <  7:220158/140‑1 (MQ=255)
                                                               aaTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGcaca                                                                             <  2:200478/137‑1 (MQ=255)
                                                               aaTTTATACTGTCGAATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCATGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTTAGCACTATTACGCACAAc                                                                           <  3:469186/139‑1 (MQ=255)
                                                                    ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTT‑TATTTTTTAAGAGGAATAAAAAAAGGGGCGAACCGGGGTTTGTTATTGACTTTTACGACCAACTGAtt                                                                      >  6:177610/1‑139 (MQ=255)
                                                                    ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTa                                                                        <  1:33828/138‑1 (MQ=255)
                                                                    ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATc                                                                      <  8:238937/140‑1 (MQ=255)
                                                                    ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTATG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATc                                                                      <  7:674806/140‑1 (MQ=255)
                                                                         gacttataAAATTTGACTGGCATATCTGTCACTCTCTCCTTCCAGGTGAACCAGTCG‑TTGTTTTTTG‑AATTTTATAAGATCTATAAACAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAAATTATCTTCAt                                                                 <  5:973313/136‑1 (MQ=255)
                                                                         gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCAt                                                                 <  7:613440/140‑1 (MQ=255)
                                                                         gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTAG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACTCACAACTTATCTTc                                                                   <  2:503947/138‑1 (MQ=255)
                                                                         gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCACCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGGGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCAt                                                                 <  5:703997/140‑1 (MQ=255)
                                                                           cGTATAAAATTCGACTGGCATATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTc                                                               <  8:711551/140‑1 (MQ=255)
                                                                           cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTc                                                               <  6:451261/140‑1 (MQ=255)
                                                                            gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCg                                                              <  6:566192/140‑1 (MQ=255)
                                                                              ataAAATTCGAGAGGCAACTCTGGCCGTCTCTACTCCCAGGTGAACCAGTCG‑TTTTTTTTTT‑AATATTATAAGAGCTATATAATTCTGTTCGAACTCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTg                                                            <  7:102876/140‑1 (MQ=255)
                                                                              ataAAATTCGACTGGCAACTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACTCACAACTTATCTTCATTCGTg                                                            <  4:279928/140‑1 (MQ=255)
                                                                              ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTT‑‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGc                                                           <  5:626347/140‑1 (MQ=255)
                                                                                   aTTCGACTGGCAAATCTTGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAAAGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCtg                                                         <  1:106334/138‑1 (MQ=255)
                                                                                   aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCtgtg                                                       <  3:810568/140‑1 (MQ=255)
                                                                                   aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCtgtg                                                       <  5:1181027/140‑1 (MQ=255)
                                                                                      cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTT‑TTTTTTTTTATTGCGATAAAAAAACGGGGGGACGCGGTTTTGTTTATCACATTTTAGCACAAAATATATTCATTGGTGGTgggg                                                      >  2:338803/1‑138 (MQ=255)
                                                                                         cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCATTCGTTTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACtt                                                  <  6:843752/140‑1 (MQ=255)
                                                                                          tGGCAAATCTTGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCa                                                <  7:786113/140‑1 (MQ=255)
                                                                                                agcTGGAACTATCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCttt                                          <  4:407172/138‑1 (MQ=255)
                                                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCtt                                           <  1:1109617/138‑1 (MQ=255)
                                                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTa                                         <  6:778533/140‑1 (MQ=255)
                                                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTa                                         <  7:597686/140‑1 (MQ=255)
                                                                                                 tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTa                                         <  4:602787/140‑1 (MQ=255)
                                                                                                   tGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAAt                                       <  6:449767/140‑1 (MQ=255)
                                                                                                     gCACTCTCTCCGGCCATGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGa                                     <  6:815848/140‑1 (MQ=255)
                                                                                                      cACTCTCTCCGGCCAGGTGATCCAGTCG‑GTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                    <  7:163600/140‑1 (MQ=255)
                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                    <  6:458739/140‑1 (MQ=255)
                                                                                                      cACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGAt                                    <  5:537385/140‑1 (MQ=255)
                                                                                                                ggCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTtgtg                          <  6:504898/140‑1 (MQ=255)
                                                                                                                  ccAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTgcg                        <  6:611759/140‑1 (MQ=255)
                                                                                                                    aGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTgcg                        <  2:427659/138‑1 (MQ=255)
                                                                                                                       tGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTaaa                   <  7:716358/140‑1 (MQ=255)
                                                                                                                       tGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGATGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTTGACTTCAGGCTTTAATGATAAGATTTGTGCGCTaaa                   <  8:310901/140‑1 (MQ=255)
                                                                                                                       gtaaCCTGTTG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTTCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTaaa                   <  7:1092539/138‑1 (MQ=255)
                                                                                                                        gAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTaa                    <  1:422742/138‑1 (MQ=255)
                                                                                                                         aaCCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTaaa                   <  1:397770/138‑1 (MQ=255)
                                                                                                                         aaCCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATa                 <  4:496674/140‑1 (MQ=255)
                                                                                                                            cAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATAc                <  2:303817/138‑1 (MQ=255)
                                                                                                                                cG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGa          <  8:200213/140‑1 (MQ=255)
                                                                                                                                   tttttttttt‑AATTTTATAAGAGATATAAAAACCGGTGCGAAAGCGGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAAt        <  5:849864/140‑1 (MQ=255)
                                                                                                                                    ttttttttG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATa       <  6:633298/140‑1 (MQ=255)
                                                                                                                                        ttttG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACTCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGAt   <  4:1084041/140‑1 (MQ=255)
                                                                                                                                        ttttG‑AATTTTATAAGAGCTATAAAAAAAGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGAt   <  3:126456/140‑1 (MQ=255)
                                                                                                                                         tttG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGATTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATc  <  8:6273/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCCGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTG‑AATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATACGATC  >  NZ_CP022686/3604425‑3604700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A