Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F13 I0 R1	230	5035.0	9125430	98.4%	8979423	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,546,400	A→C	18.6%	L93R (CTG→CGG)	waaG ←	glycosyltransferase family 4 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,546,400	0	A	C	18.6%	257.7 / 17.8	177	L93R (CTG→CGG)	waaG	glycosyltransferase family 4 protein
Reads supporting (aligned to +/- strand): ref base A (83/53); new base C (4/32); total (87/90)
Fisher's exact test for biased strand distribution p-value = 4.54e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATACAT  >  NZ_CP022686/2546262‑2546538

tgtgTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAg                                                                                                                                            >  1:406138/1‑140 (MQ=255)

  tgtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGcc                                                                                                                                          >  6:443426/1‑140 (MQ=255)

   ggcGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGc                                                                                                                                           <  8:154078/136‑1 (MQ=255)

    tCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCTCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGcccc                                                                                                                                        >  6:843459/1‑140 (MQ=255)

    tCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGcccc                                                                                                                                        >  6:152897/1‑140 (MQ=255)

    tCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGcccc                                                                                                                                        >  4:910143/1‑140 (MQ=255)

         ttGCCCTGTTCGAAGGGTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCTGCGGC‑ATAATAAACGTCCAGCCCCGGCAt                                                                                                                                   <  1:698314/140‑1 (MQ=255)

          tGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAAAGTGACGGCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCa                                                                                                                                    <  7:4700/138‑1 (MQ=255)

            cccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                                >  5:230540/1‑140 (MQ=255)

                 ttCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑Taa                                                                                                                          >  4:403300/1‑140 (MQ=255)

                 ttCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTAAGGCGCTAGAAAAAGCCTTTCTCCTGCGCAACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGAATATTAT‑Taa                                                                                                                          >  6:657790/1‑140 (MQ=255)

                  tCGAAGGGTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGGCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑Taaa                                                                                                                         <  5:757986/140‑1 (MQ=255)

                   cGAAGGTTGCCCGCTCACAGGCGGCATAATGGCGCTAACGTGACGTCAGGCGATAGAAAAAGCCTATCTCCTGCGCTAATTTCTCGGCTAAACAAA‑CATACGCGCC‑ATAATGACCGTCCCGCCCCGGCCTTgtat‑tat                                                                                                                          >  8:312818/1‑137 (MQ=255)

                   cGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGTCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑Taa                                                                                                                          >  8:672480/1‑138 (MQ=255)

                      aGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCa                                                                                                                     >  2:644186/1‑140 (MQ=255)

                        tttGCCCGCTCAATGGCGGAATATTGGCGATAACGTGACGACTGGCGATATAAAAAGCCTTTTTCCTGCCCTACTTTCTCGGCATAACAAA‑CATCAGCGGA‑ATAATAAACGTCCAGCCCCGGAATTTTAT‑TAAATCCAAc                                                                                                                   <  4:480654/139‑1 (MQ=255)

                          tGCCCGCTAAATGGCGTCATAATGGCTATAACGTGACGTCAGGCGATAGAAAAAGCATTTCTCCTGCGCAACTTTCTCGGCATAACAAA‑CATCAGCGCA‑ATAAAAAACTTCCAGCCCCGGCATTTTAT‑TAAATCCAACGa                                                                                                                 <  2:306481/140‑1 (MQ=255)

                          ggCCCGCTCAAAGGCGGAATAATGGCTATACCGGTACGTCAGGCTATAGAAAAAGCCTTTCTCCTGCGATACTTTCTCGGCATAACAAC‑CATCCGGGCC‑AAAATAACCGTCCCGCCCGGGCATTTTAT‑AAAAACCAACGa                                                                                                                 <  6:91912/139‑1 (MQ=255)

                          ggCCCGCTCAAAGGCGACATAATGGCGATACCGTGCCGTCCGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAAAAAA‑CATCAGCGGC‑ATAATAAACGGCCAGCCCGGGAATTTTAT‑TAAATCCAACGa                                                                                                                 <  2:822441/139‑1 (MQ=255)

                           gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGAc                                                                                                                >  5:44730/1‑140 (MQ=255)

                           gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGAc                                                                                                                >  2:918942/1‑140 (MQ=255)

                           gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGAGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGAc                                                                                                                >  4:300187/1‑140 (MQ=255)

                             ccccacAACGGCGGAATAATGCCCATAACGGGACGTCTGGCGATACAAACAGCCTTGGCCCTGCGCTACTTTCTCGGAATAACAAA‑CAGCCGCGTA‑ATAAAAAACGCCCACCCCGGGATTTTTAT‑TAAATCCAACGActc                                                                                                              <  2:127294/135‑1 (MQ=255)

                                cTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTAt                                                                                                           >  5:45158/1‑140 (MQ=255)

                                   aaaGGGGGCATAAGGGCGAAACCGTCACGTCAGGCGATACAAAAAGCCTTTCTCCTGCGCTACTTTCGCGCAATAACAAA‑CATC‑CCGGCAATAATAAACGCCCAGCCCCGGCATTTTAT‑TAAACCCCACGACTCTATc                                                                                                          <  8:922059/138‑1 (MQ=255)

                                   aaaGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTGTCTCGGCATCACAAA‑CATCAGCGGC‑ATAATAACCGTCCCGCCCCGGCATTTGAT‑TACATCCATCGACTCTAACgc                                                                                                        >  6:1117411/1‑139 (MQ=255)

                                   aaaGCCGGAATAATGGCGATACCGTCACGACCGGCGAATTAAAACGCCTTTACCCTGCGCTACATGCTCGGCTTCACAAA‑CCCCACCGGC‑ATAATAAACGTCCAGCCCGGGCTGTTTAT‑TAAATCCAACGACTCTATc                                                                                                          <  8:350073/138‑1 (MQ=255)

                                    aaGGTGTCATAATTGCGATAACGTTACGTAAGGCGATAGAAAAAGCCTTTCACCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCTGC‑ATAATAAACGGCCAGCCCCGGCATTTTAT‑TAAAACCAACGACTCTATCGAc                                                                                                       <  6:393309/140‑1 (MQ=255)

                                       gcggcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAACCGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACggg                                                                                                    <  4:227877/140‑1 (MQ=255)

                                       gcggcATAAGGGCGATAACGTCCCGGCCGGCGATACAAAAAGCCTTTCGCCTGCGCTACTTTCGCGGCATAACAAC‑AATCTGCGCC‑AAAATAAACGTCCCGCCCCGGAATTTATT‑TAAAACCAACGACTCTATCGACg                                                                                                      <  8:251784/138‑1 (MQ=255)

                                          gcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGgtg                                                                                                 >  6:339161/1‑140 (MQ=255)

                                          gcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑GAAATCCAACGCCTCTATCGACGGGgtg                                                                                                 >  4:801262/1‑140 (MQ=255)

                                             tAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTATCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCTACGGGgttt                                                                                                >  7:273837/1‑136 (MQ=255)

                                             tAATGGCGATAACGTGACGTCAGGAGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACTTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGgtgg                                                                                                >  8:239799/1‑137 (MQ=255)

                                                 ggCGATAACGTGACGTCAGGCGATAGAAAAAGCATTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACgg                                                                                          >  3:877042/1‑139 (MQ=255)

                                                    gATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCATGTaaa                                                                                       >  3:653111/1‑140 (MQ=255)

                                                     aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTaaaa                                                                                      >  3:1149310/1‑139 (MQ=255)

                                                     aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  3:543398/1‑140 (MQ=255)

                                                     aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  1:136831/1‑140 (MQ=255)

                                                     aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  1:26020/1‑140 (MQ=255)

                                                     aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  6:884641/1‑140 (MQ=255)

                                                     aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑AAAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  1:426095/1‑140 (MQ=255)

                                                          gTGCCGTCAGGCTATATAAAAAGCCTTTCTCCTCCGCTACTTTCTCGTCATAACAAA‑CATCAGCGGC‑ATAATAACCTTCCACCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGtttt                                                                                 <  2:705657/140‑1 (MQ=255)

                                                                acAGGCGAAAGAAAACCCCTTACACCTGCGCAACTTTCCCGCCATAACACA‑ACTCCGCGGA‑ATAATACACGCCCCCCCCCGGCATTTTAT‑AAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCAcc                                                                           <  5:915884/139‑1 (MQ=255)

                                                                 cAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCAccc                                                                          >  2:413477/1‑140 (MQ=255)

                                                                    gCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCAcccc                                                                         >  7:98266/1‑137 (MQ=255)

                                                                      taTACAAACACCCTTTCTCCTGCGATACTTTCTCGCCATAACAAA‑CATCACCGGA‑ATAATAACCGTCAATCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCa                                                                     <  4:272385/139‑1 (MQ=255)

                                                                       aTAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCTGCGGC‑ATAATAATCGTCGAGCCCCGGCTTTTTTT‑TAAATCCAACGACTCTATCGAAGGGGTGTTCACGTTATTGTTTGTGCACCCAGGCaa                                                                    >  4:686759/1‑140 (MQ=255)

                                                                        tAGAAAACGCCTTTCTCCTGCGCTACTTTCTCGGAATAACAAA‑CATCCCCGGC‑ATAATAACCGCCCAGCCCCGGCATTTTAT‑TAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaa                                                                   <  6:280709/140‑1 (MQ=255)

                                                                        tAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAATTGTTTTTGCACCCAGGCaaa                                                                   >  2:1122210/1‑140 (MQ=255)

                                                                         aGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCGCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaaa                                                                  >  2:1053024/1‑140 (MQ=255)

                                                                         aGAAAAAGCCGTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaa                                                                    >  8:634935/1‑138 (MQ=255)

                                                                          taaaaccccTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGCC‑ATAAAAAACGTCCCCCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAat                                                                 <  2:1108588/133‑1 (MQ=255)

                                                                              atcccTTTCTCCTGCGCAACTTTCCCGGAATAAAAAA‑CATCACCGCC‑ATAATACACGCCCAGCCCGGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAatat                                                               <  8:532641/135‑1 (MQ=255)

                                                                              aaGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑AGAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTc                                                             >  6:796435/1‑140 (MQ=255)

                                                                                 ccTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTcc                                                            >  7:118215/1‑138 (MQ=255)

                                                                                  cttccaccTGCCCTACTTTCGC‑GCAAAACAAACCACCCCCGCA‑AAAAAAAACGCCCCCCCCCGCCATTTTAT‑AAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCg                                                           <  7:33584/132‑1 (MQ=255)

                                                                                  cTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACACAGGAAAAATATTCCGCa                                                         >  5:1096238/1‑140 (MQ=255)

                                                                                    ttCACCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTAACGTAAATGATTTTGCACCAAGGCAAAATATTCCGCAtt                                                       >  3:304767/1‑140 (MQ=255)

                                                                                     tctcCTGCGCTACTATCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTATTGCACCCAGGCAAAATATTCCGCATTg                                                      >  1:1072163/1‑140 (MQ=255)

                                                                                       cccTGCGCTATTTTCTCGGAATAACAAA‑CATCCGCTCA‑ATAATAACCGTCCCGCCCCGGAATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                                    <  3:245922/139‑1 (MQ=255)

                                                                                            cgcTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGt                                               >  1:1100056/1‑140 (MQ=255)

                                                                                              ctcatttCTCGGAATAAAAAA‑CATCAGCGTC‑ATACTAAACGTCCCGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGt                                               <  8:993817/134‑1 (MQ=255)

                                                                                               taatttCTCGGCATAACAAA‑CATCAGCGTA‑ATAATAAACGTCCCGCCCCGGATTTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                              <  8:851291/135‑1 (MQ=255)

                                                                                               aaCTTTCTGGGCATAAAAAA‑CATCAGCGGC‑ATAATAAACGCCCCGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGAt                                            <  4:372941/139‑1 (MQ=255)

                                                                                                 cTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTg                                          >  3:450789/1‑140 (MQ=255)

                                                                                                    tctcGGCATAACAAC‑CATCAGCGGC‑ATAATAAACGTCCCGCCCCGGCATTTTAT‑TAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTa                                       <  4:999694/140‑1 (MQ=255)

                                                                                                            aaacaaa‑caTCCGCGGA‑AAAATAAACGTCCCCCCCCGCAATTTTAT‑TAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGAttt                               <  3:76001/139‑1 (MQ=255)

                                                                                                             aacaaa‑caTCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGATTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTa                              >  6:583246/1‑140 (MQ=255)

                                                                                                                aaaccctCAGCGCA‑ATAATAACCGGCC‑CCCCCCGCATTTTTTATAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                            <  2:468844/134‑1 (MQ=255)

                                                                                                                 aa‑caacAGCCGC‑AAAAAAACCGTCCCCCCCCGCAATTTTAT‑TAAATCACACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                            <  8:1008511/138‑1 (MQ=255)

                                                                                                                 aa‑caTCCGCGGC‑ATAATAAACGGCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          <  4:441316/140‑1 (MQ=255)

                                                                                                                 aa‑caTCAGCGGC‑ATAATAACCGTCCACCCCCGGCATTTTAT‑TAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          <  2:61878/140‑1 (MQ=255)

                                                                                                                  acaaTCAGCGGC‑ATAATAACCGTCCAGCCCCGGCATTTTAT‑TAAATCAAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          <  1:950883/137‑1 (MQ=255)

                                                                                                                  aaaaTCCGCGGA‑ATAATAACCGTCCAGCCCCGTCTTTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          <  3:1102148/137‑1 (MQ=255)

                                                                                                                    caTCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGc                        >  1:846442/1‑140 (MQ=255)

                                                                                                                     aTCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCa                       >  1:218031/1‑140 (MQ=255)

                                                                                                                        aGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACtt                    >  1:634805/1‑140 (MQ=255)

                                                                                                                          cGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg                  >  6:209096/1‑140 (MQ=255)

                                                                                                                          cGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCAAACGACTCTATCGACGGGGTTTTAACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGAATGGTATGCGATTTAACCGGCACtatt                  >  2:340004/1‑137 (MQ=255)

                                                                                                                           gcc‑ATAATAACCGCCCACCCCCGGCATTTTAT‑TAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACttt                   <  8:581376/136‑1 (MQ=255)

                                                                                                                            gC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCAAGTAAATGTTTTTGAACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg                  >  7:360852/1‑138 (MQ=255)

                                                                                                                            cc‑ATAATAAACGTCCACCCCCGGCTTTTTAT‑TAAAACCAACGACGCTATCGTCGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGACCGTGATTGGTATGCGATTTAACCGGCACTTTGAt                <  6:867394/139‑1 (MQ=255)

                                                                                                                             c‑ATAATAAACGTCCAGCCCCTGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTAGGCGATTTAAACGGCACTTTGagc               >  5:832369/1‑138 (MQ=255)

                                                                                                                             c‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               >  6:1018049/1‑140 (MQ=255)

                                                                                                                             c‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  3:666700/140‑1 (MQ=255)

                                                                                                                              cttaataaAAGTACCGCCCCGGCATTTTTT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGAACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  6:72744/138‑1 (MQ=255)

                                                                                                                              aataataAACTTCAAGCCCCGTCTTTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  1:299561/139‑1 (MQ=255)

                                                                                                                              aaaaataaCCGGCCACCCCGGGCATTTTAT‑AAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  3:16522/137‑1 (MQ=255)

                                                                                                                                  ataaACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTACCCGGCACTTTGATCAg             >  8:113792/1‑138 (MQ=255)

                                                                                                                                     aaggTCCCGCCCCGGCTTTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaa        <  5:702463/137‑1 (MQ=255)

                                                                                                                                      ccGTCCCGCCCCTGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa       <  6:978684/139‑1 (MQ=255)

                                                                                                                                      ccGTCCCGCCCCGTATTTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa       <  5:963787/139‑1 (MQ=255)

                                                                                                                                      ccGTCCAGCCCCGGCTTTTTAT‑TAATTCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCTGCACTTTGATCAGTTCaaa       <  5:657790/139‑1 (MQ=255)

                                                                                                                                      acttCCCGCCCCGCAATTTAAT‑AAAAACCAACGACTCTATCGACGGGGTGTGCACGTAAATGTTTTTGCACCCATGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa       <  3:300187/137‑1 (MQ=255)

                                                                                                                                      aCGTCCACCCCCGGCTTTTTAT‑TAAATCCAACGACTCTATCGACGGTGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa       <  2:221017/140‑1 (MQ=255)

                                                                                                                                        gcccAGCCCCGCAATTTTAT‑TAAATCAAACGACTCTATCGACGGGGTGTTTACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATa     <  4:772544/138‑1 (MQ=255)

                                                                                                                                        gTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          >  5:1077680/1‑119 (MQ=255)

                                                                                                                                        gTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          <  6:1077680/119‑1 (MQ=255)

                                                                                                                                        gTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATa     >  4:613868/1‑140 (MQ=255)

TGTGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAA‑CATCAGCGGC‑ATAATAAACGTCCAGCCCCGGCATTTTAT‑TAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATACAT  >  NZ_CP022686/2546262‑2546538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A