Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F36 I0 R1	107	8366.7	6299831	98.3%	6192733	135.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,546,400	A→C	17.9%	L93R (CTG→CGG)	waaG ←	glycosyltransferase family 4 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,546,400	0	A	C	17.9%	194.2 / 8.2	126	L93R (CTG→CGG)	waaG	glycosyltransferase family 4 protein
Reads supporting (aligned to +/- strand): ref base A (61/38); new base C (3/23); total (64/62)
Fisher's exact test for biased strand distribution p-value = 4.34e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATA  >  NZ_CP022686/2546265‑2546535

     cTTGCCCTGAGCGAAGGGTGCCCGCTCAACGGAGGCATATTGGCGATATCGTGACGTCTGGCGATATAACAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGGCCAGCCCCGGCa                                                                                                                                 <  7:200962/140‑1 (MQ=255)

      ttGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAt                                                                                                                                <  6:233331/140‑1 (MQ=255)

       tGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGGCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtt                                                                                                                               <  6:448917/140‑1 (MQ=255)

         cccTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCATGCGATAGAAAAAGCCTTTCTCATGCGCTACTTTCTCGGCATAACAAACATCAGCTGCATAATAAAAGTCCATCCCCTGCAtttt                                                                                                                             >  6:459315/1‑140 (MQ=255)

         accagTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACTTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                             >  8:341771/5‑140 (MQ=255)

           cTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCAtttt                                                                                                                             >  4:125042/1‑138 (MQ=255)

             tctCGAAGCTAGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCTGCATTtt‑at                                                                                                                          <  2:236044/136‑1 (MQ=255)

              ttCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTaa                                                                                                                       >  5:584143/1‑140 (MQ=255)

               gcgccggTTGCCCGCTCCACGGCGGCATAATGGCGATCACGTGCCGGCCTGCGATCGAACACGCCTTTCTCCTGCGCTCCGTTATCGGCATAACAAACATCAGCGGCATAATAACCGGCCAGCCCCGGCATTtt‑atta                                                                                                                        <  4:711797/133‑1 (MQ=255)

                cGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGATACTTTCTCTGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTaa                                                                                                                       >  2:136695/1‑138 (MQ=255)

                      ttGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACg                                                                                                               >  8:70883/1‑140 (MQ=255)

                       ggCCCGCCAAAAGGCGGAATAATGGCTATAACGGGACGGCCGGCTATAAAAAACCCCTTTCCCCTGCGCTACTTTCCGGGCATAACAAACATCCGCGGAATAAAAAACGCCCCGCCCCGGCATTTT‑ATTAAACCCCAc                                                                                                                <  2:281327/137‑1 (MQ=255)

                        gCCCTCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTGTCGCGGGATAACAACCTGCTGCGGCTTACTACACGTCCAGCCCGGGCTTTTT‑ATTAATTcccccgcc                                                                                                             >  6:187081/1‑134 (MQ=255)

                        gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGAc                                                                                                             >  7:363629/1‑140 (MQ=255)

                        gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGAc                                                                                                             >  6:407521/1‑140 (MQ=255)

                          ccgataaaaGGCGGAATATTGGCTATAACGGGACGTACGGCTATAGAAAAACCATTTCTCCTGCGCTACTTTCTCGGCAAAACAAACATCAGCGGAATAAAAACCGCCCCCCCCCGGCTTTTT‑ATTAAATCCAACGAc                                                                                                             <  4:518911/132‑1 (MQ=255)

                          ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGAc                                                                                                             >  1:287366/1‑138 (MQ=255)

                              tCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑AGTAAATCCAACGACTCTATc                                                                                                       >  8:235626/1‑140 (MQ=255)

                                 atggcggcATATTGCCGATCCCGTTCCGTCAGGCGATAGAAAACGCCTTGATCCTGCGCAACTTTCTCTCAATAACAAACCTAAGCGTCATAATAAACGGCCCGCCCGGGATTTTT‑ATTAAATCCAACGACTCTATCg                                                                                                      <  4:715195/136‑1 (MQ=255)

                                 aaGGCGTCATAATGGCTATACCGTGCCGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAAAACAAACATCCGCGGCAAAATAAACGGCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGAc                                                                                                    <  8:53408/140‑1 (MQ=255)

                                 aaGGCGTAATAATGGCTATACCGTGCCGTCCGGCTATATAAAACGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAAAAAACGTCCCGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGAc                                                                                                    <  6:183757/140‑1 (MQ=255)

                                  aGGCGGAATATTGGCTATACCGTCACGTCAGGCGATAGAAAACGCCTTTCTCCTCCGCTCTTTCCTCGCAATAACAAACATCCGCGGAAAAATAAACGACCCGCCCCGGAATTTT‑ATTAAATCCAACGACTCTATCGa                                                                                                     <  4:390088/138‑1 (MQ=255)

                                    ggggaatatTGGCGATAACGTGACGTCTGGCTTTATAAAAAGCGTTTCTCCTGCGCTCCTTTATCGCCATAAAAAACATCCGCGTCTTAATAAACGGCCAGCCCCGGATTTTT‑ATAAAAACCAACGACTCTATCGACggg                                                                                                 <  6:597990/135‑1 (MQ=255)

                                        cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGgtgt                                                                                             >  5:229809/1‑140 (MQ=255)

                                         aTAATTGCGATACCTTGACGGCCGGCCATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCAAAACAAACATCCGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGgtg                                                                                              <  4:354562/138‑1 (MQ=255)

                                         aTAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGgtg                                                                                              >  2:39331/1‑138 (MQ=255)

                                          tAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGgtgt                                                                                             >  2:776940/1‑138 (MQ=255)

                                          tAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCATTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAACCGTCCAGCACCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGgtgt                                                                                             >  4:294790/1‑138 (MQ=255)

                                                 gATAACTTTACGTCTGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTGGGCATAACAAACATCAGCGGCATAATAACCGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTaaa                                                                                    <  6:657816/140‑1 (MQ=255)

                                                  aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGCGGTGTTCTCGTatat                                                                                   >  5:516494/1‑140 (MQ=255)

                                                    aaCGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                   >  3:587030/1‑138 (MQ=255)

                                                    aaCGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                   >  4:219360/1‑138 (MQ=255)

                                                    aaCGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCACGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGGGTTCACGTAAAt                                                                                   >  2:601748/1‑138 (MQ=255)

                                                          aCGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCAACTTTATCGGCATAACAAACATCAGCGGCATAATAAACGTCCCGCACCGGCATTTT‑ATTACATCCAACGACTCTATCTACGGGGTGTTCACGTAAATGTTTTTGc                                                                           >  5:15130/1‑140 (MQ=255)

                                                            ggcccgCGTTAGAAAAAGCGATTCACCTGCGCTACTCTCCCGGCATACCAACCTTAAGCGGCATAAAAACCGTCCAGCCCCGTCAATTT‑ATTAAAACCAACGACTCTATCGACGGGGGGTTCACGTAAATGATTTTGCAc                                                                         <  8:563549/135‑1 (MQ=255)

                                                            gTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGc                                                                           >  4:521478/1‑138 (MQ=255)

                                                              cAGGCTATATAAAAAGCCTTTCACCTGCGCTACTTTCTCGCCAAAACAAAAATCAGCGGAAAAAAAACCGTCCAGCCCCGCCATTTT‑ATAAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCAccc                                                                       <  6:584143/140‑1 (MQ=255)

                                                                 gCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAgg                                                                    >  5:760820/1‑140 (MQ=255)

                                                                 cctaTACAAAACGCCTTGCCCCTGCCATAATTTCCCGGAAAAACAACCACCCGCGGCAAAAAACACGCCCCCCCCCGCCATTTT‑ATAAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAgg                                                                    <  7:689381/137‑1 (MQ=255)

                                                                 ccgaaaaaaaaaCCATTACACCTGCGCACCATTCTCGCCATAACAACAACCCCCGCCAAAAAAAACGACCCCCCCCGGCATTTA‑ATAAAACCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAgg                                                                    <  6:600195/133‑1 (MQ=255)

                                                                   tatatAAAACGCCTTTCTCCTGCGCAAATTTCTCGCCAAACCAAAAATCAGCGGCATAATAAACGCCCCCCCCCGGAATTTT‑ATAAAAACCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAgg                                                                    <  1:436962/137‑1 (MQ=255)

                                                                   taTACAAAACGCGTTGCTCCTGCGCTACTTTCTCGCCAAAAAAAACATCCGCGCCATAATAAACGCCCACCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGGGTTCACGTAAATGTTTTTGCACCCAgg                                                                    <  3:78560/137‑1 (MQ=255)

                                                                     tAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCa                                                                  <  4:457769/138‑1 (MQ=255)

                                                                      aGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCCGCGGCATAATAAACGTCCAGCCACGGCATTTT‑ATTAAATCCAACGCCTCTATCGACGGGGTGTTCTCGTAAATGTTTTTGCACACAGGCaaaa                                                               >  8:652151/1‑140 (MQ=255)

                                                                        aaaaaGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCCCCCAGGCaaaa                                                               >  1:158727/1‑138 (MQ=255)

                                                                        aaaaaGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAata                                                             >  6:206332/1‑140 (MQ=255)

                                                                          aaaGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAata                                                             >  4:540284/1‑138 (MQ=255)

                                                                             gCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATAGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAACATATTc                                                          >  2:748330/1‑138 (MQ=255)

                                                                                tttCACCTCCGCAACTTTCTCGGAAAAACAAACACCCGCGCCAAAAAAAACGCCCACCCCGGGAATTTT‑ATAAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCAt                                                     <  5:260777/140‑1 (MQ=255)

                                                                                  tctcCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTg                                                   >  5:474055/1‑140 (MQ=255)

                                                                                  tccccTGCGCAACTTTCTCGCAAAAACAAAAAACAGCGCAAAAATAAACGGCCCCCCCCGTAATTTT‑ATAAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCAt                                                     <  3:788643/135‑1 (MQ=255)

                                                                                    gccggcgcTACTTTCTCGGCATAACAAACTTCCGCGCCAAAATACACGCCCCCCCCGGGAATTTT‑ATTAAACCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTGCGCATTgcg                                                 <  7:355312/136‑1 (MQ=255)

                                                                                      cTGCGCTACTTTCTCGGAATAACACACCTCACCGCCATAATAAACGCCCCCCCCCGCCATTTA‑ATAAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                                 <  4:173383/138‑1 (MQ=255)

                                                                                      cTGCGCAAATTTCTCGGCATAACCAACATCAGCGGCATAATAACCGGCCAGCCCGGTCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGcc                                               <  8:391967/140‑1 (MQ=255)

                                                                                        gcgcTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCg                                             >  7:194292/1‑140 (MQ=255)

                                                                                        gcgcTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCg                                             >  5:532991/1‑140 (MQ=255)

                                                                                         cgcTACTTTCTCGGCATACCAAACATCAGCGGCATAATAAACGTCCCGCCACGGCAGTTT‑ATTAAATCCATCGACTCTATCGACGGGGTGGTCACGTAAATGTTTTTGCCCCCAGGCAAAATATTCCGCATTGCGCCCgg                                            >  8:334935/1‑139 (MQ=255)

                                                                                         cgcTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGt                                            >  7:621176/1‑140 (MQ=255)

                                                                                          gcTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCg                                             >  3:266723/1‑138 (MQ=255)

                                                                                            tACTTTCTGGGCATAACAAACATCAGCGGCAAAATAACCGTCCCGCCCCGGAATTTT‑ATTAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                           <  4:382892/138‑1 (MQ=255)

                                                                                            tACTTTCTGGGAATAAAAAACATCAGCGGCATAAAAAACGTCCAGCCCCGGAATTTT‑TTTAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                           <  1:39331/138‑1 (MQ=255)

                                                                                            tACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGGCCCGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                           <  2:494102/138‑1 (MQ=255)

                                                                                            tACTTGCTGGGCATAACAAACAGCAGCGGCATAATAAACGGCCCGCCCCGGAATTTT‑ATTAAATCAAACGACTCTATCGCCGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGAt                                         <  8:83636/140‑1 (MQ=255)

                                                                                            aaatttCTCGGCATAAAAAACATCCGCGGAATAATAAACGTCCACCCCCTGCTTTTT‑ATTAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTg                                           <  3:121868/135‑1 (MQ=255)

                                                                                            aaCTTTCTGGGAAAAACAAACACCACCGCAATAATAACGGCCCAGCCCCGGATTTTT‑ATAAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGAt                                         <  5:312145/139‑1 (MQ=255)

                                                                                              cTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTtt‑atta                                                                                                                        <  5:95167/59‑1 (MQ=255)

                                                                                              cTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTtt‑atta                                                                                                                        >  6:95167/1‑59 (MQ=255)

                                                                                              cTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATAGCGCCCGTGATTg                                       >  5:64071/1‑140 (MQ=255)

                                                                                                ttCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTg                                       >  3:517423/1‑138 (MQ=255)

                                                                                                 tctcGGCAAAACAAACATCAGCGGCATAATAACCGTCCCGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTgg                                      <  1:502859/138‑1 (MQ=255)

                                                                                                 gagcGGAATAAAAAACAACCGCGCCAAAAAAAACGTCCCCCCCCGGCATTTT‑ATAAAACCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTgg                                      <  3:103325/135‑1 (MQ=255)

                                                                                                 actcGGCATAAAAAACAGCCGCGGCATAATAACCGTCCAGCCCCGGAATTTT‑ATTAAATCCCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTgg                                      <  1:549553/137‑1 (MQ=255)

                                                                                                   tcGGCATAAAAAACATCAGCGTCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCCACGACTCTATCGACTGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTa                                    >  4:441887/1‑138 (MQ=255)

                                                                                                     ggCATAACAACCATCAGCGGAATAATAAACGCCCCGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATg                                  <  3:433179/138‑1 (MQ=255)

                                                                                                       aaTAACAAACATCAGCGGCATAAAAAACGTCCAGCCCCGGCTTTTT‑ATAAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGAt                              <  7:587374/139‑1 (MQ=255)

                                                                                                              aacaTCAGCGGCATAATAAACGTCAAGCCCCGGAATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                       <  8:330769/140‑1 (MQ=255)

                                                                                                              aacaTCAGCGGCAT‑ATAAACCTTCACCCCCCGGATTTTAATTAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                       <  8:739820/140‑1 (MQ=255)

                                                                                                                 aTCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGCCTCTATCGACGGGGTGTTCTCGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGGGATTGGTATGCGATTTAACCGGCa                    >  6:328620/1‑140 (MQ=255)

                                                                                                                   cAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCa                    >  4:240137/1‑138 (MQ=255)

                                                                                                                      cGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCACCGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCCCTTTg               >  5:13121/1‑140 (MQ=255)

                                                                                                                      cGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACACAGGCAAAATATTCCGCATTGCGCACGTGATTGGTATGCGATTTAACCGTCACtt                 >  3:265609/1‑138 (MQ=255)

                                                                                                                      cGGCATAATAAACGTCCAGCACCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg               >  6:347994/1‑140 (MQ=255)

                                                                                                                        gCATATTAACCGTCCCCCCCCGCCATTTA‑ATTAAATCCAAAGACTCTATCGAAGGGTTGTTCACTTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGAGATTTAACCGGCACTTTg               <  4:199988/138‑1 (MQ=255)

                                                                                                                        gCATATTAACCGCCCCGCCCCGTCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg               <  2:618166/138‑1 (MQ=255)

                                                                                                                        gCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg               >  3:61327/1‑138 (MQ=255)

                                                                                                                        ccATAATAACCGCCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTg               <  3:224976/137‑1 (MQ=255)

                                                                                                                         cATAATAAACGTCCCGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa              <  2:295950/138‑1 (MQ=255)

                                                                                                                         cATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa              <  2:769226/138‑1 (MQ=255)

                                                                                                                         aataataACTGTACAGACCGGGAATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTTATTGGTATGCGATTTAACCGGCACTTTGa              <  2:769227/137‑1 (MQ=255)

                                                                                                                         aataataAACGTCCAGCCCGGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa              <  3:771156/137‑1 (MQ=255)

                                                                                                                         aataataAACGGACCGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc            <  6:331195/139‑1 (MQ=255)

                                                                                                                           taataaACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCTTGATTGGTATGCGATTTAACCGGCACTTTGATCAg          >  6:644420/1‑140 (MQ=255)

                                                                                                                            aataaACGTCCACCCCCGCCATTTT‑AAAAAAACCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt         <  8:216965/140‑1 (MQ=255)

                                                                                                                                 cggaccACCCCCGCCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCa      <  2:278637/134‑1 (MQ=255)

                                                                                                                                  cgaccAGCCCCGGCATTTA‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAAt   <  8:588945/137‑1 (MQ=255)

                                                                                                                                   gTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATa  >  6:139634/1‑140 (MQ=255)

                                                                                                                                   gTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATAAGTTCAAATa  >  6:479660/1‑140 (MQ=255)

                                                                                                                                     ccAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATa  >  2:810345/1‑138 (MQ=255)

                                                                                                                                     ccAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAACGGCACTTTGATCAGTTCAAATa  >  2:481272/1‑138 (MQ=255)

                                                                                                                                     ccAGCCCCGGCATTAT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATa  >  3:532303/1‑138 (MQ=255)

GTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTACTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCAGCCCCGGCATTTT‑ATTAAATCCAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATA  >  NZ_CP022686/2546265‑2546535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A