Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,546,400 A→C 15.0% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,546,4000AC15.0% 287.8 / 10.6 181L93R (CTG→CGG) waaGglycosyltransferase family 4 protein
Reads supporting (aligned to +/- strand):  ref base A (86/62);  new base C (2/30);  total (88/93)
Fisher's exact test for biased strand distribution p-value = 2.43e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

TGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATACAT  >  NZ_CP022686/2546264‑2546538
                                                                                                                                         |                                                                                                                                             
tgtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTTACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑Gcc                                                                                                                                           >  2:821157/1‑140 (MQ=255)
tgtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑g                                                                                                                                             >  3:790849/1‑138 (MQ=255)
 gtCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑Gccc                                                                                                                                          <  5:608676/140‑1 (MQ=255)
  tCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACCAACATCAGCGGCATAATAAACGTCCA‑Gcccc                                                                                                                                         >  7:29291/1‑140 (MQ=255)
  tCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTACTCGCCATAACAAACATCAGCGGCATAAGAAACGTCCA‑Gcccc                                                                                                                                         >  8:1004246/1‑140 (MQ=255)
    ggCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACTTCCA‑Gcccc                                                                                                                                         >  3:811943/1‑138 (MQ=255)
       ttGCCCTGTTCGAAGGTTGCCCCCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCAGAACATACATCAGCGGCATATTAAAGGGCGA‑GCCAGGGCA‑t                                                                                                                                   >  6:540258/1‑140 (MQ=255)
            cTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTttat                                                                                                                              >  1:1082336/1‑140 (MQ=255)
               ttCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTaa                                                                                                                           >  1:251903/1‑140 (MQ=255)
                tctaaGGTTGCCCGATCAATGGCGGAATAATGGCTATAACGTGAGGTAAGTCGATATAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCTGCGGCATAATAAACGTCCA‑GCCCCGGAA‑TTTTATTaaa                                                                                                                          <  6:200644/137‑1 (MQ=255)
                 cGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTaa                                                                                                                           >  3:153492/1‑138 (MQ=255)
                 cGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTaa                                                                                                                           >  3:54427/1‑138 (MQ=255)
                 cGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTaa                                                                                                                           >  3:63873/1‑138 (MQ=255)
                    aGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATcaa                                                                                                                      >  8:352845/1‑138 (MQ=255)
                      gTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑Ca                                                                                                                     >  3:1271703/1‑138 (MQ=255)
                        gccccGCTCAACGGCGGAATAATGCCGATAACGGGACGGCAGGCGATATAAAATGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGCCATAATAAACGTCCA‑GCCCCGGAA‑TTTTATTAAATC‑CCACGa                                                                                                                 <  8:1254877/138‑1 (MQ=255)
                         gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCATCGGCATAATAAACGTCCC‑GCCCCGGCA‑GTTTATTAAATC‑CAACgcc                                                                                                                >  1:59693/1‑138 (MQ=255)
                         gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAACCGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGAc                                                                                                                >  8:286337/1‑140 (MQ=255)
                         gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGAc                                                                                                                >  1:435091/1‑140 (MQ=255)
                         gCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCGTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAAGAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGAc                                                                                                                >  7:921815/1‑140 (MQ=255)
                           ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGAc                                                                                                                >  3:1178056/1‑138 (MQ=255)
                           ccGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTATCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTTAATC‑CAACGAc                                                                                                                >  3:392836/1‑138 (MQ=255)
                              cTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTAt                                                                                                           >  2:1292202/1‑140 (MQ=255)
                               tCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATc                                                                                                          >  7:8609/1‑140 (MQ=255)
                                cAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTAt                                                                                                           >  3:444497/1‑138 (MQ=255)
                                   tggcggcATAATGTCTATAACGTTACTTCAGTCGATATAAAACGCCTTTCTCCTGCGCTA‑CTTTCTCGGCAAAACAAACAACAGCGGAATAATAACCGACCA‑GCCCCGTCA‑TTTTATTAAATC‑CAACGACTCTATCGa                                                                                                        <  4:955690/137‑1 (MQ=255)
                                     gcggcATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACg                                                                                                      >  4:1289017/1‑138 (MQ=255)
                                         cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGgtgt                                                                                                >  1:423987/1‑140 (MQ=255)
                                         cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGgtgt                                                                                                >  1:1302189/1‑140 (MQ=255)
                                         cATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCAGAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGCCGGGgtgt                                                                                                >  6:963291/1‑140 (MQ=255)
                                          atatTGGCTAAACCGTGACGTCAGGCGATATAAAAAGCCTTTCTCCTGCGCTA‑CTTTCCCGGCATAACAAACATCAGCGCAATAATAAACGACCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGtt                                                                                               <  2:147854/140‑1 (MQ=255)
                                          aTAATGGCTAAAACGGGACGTCTGGCTATTCAAAACGCCTTTCCCCGGCGCTA‑CTTTCTCGGAAAAACAAACAGCCGCGCCATAATAACCGCCCC‑GCCCCGGCA‑TTTTATTAAACC‑CAACGACTCTATCGACGGGGTGtt                                                                                               <  8:765548/140‑1 (MQ=255)
                                                 cGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTaa                                                                                        >  8:618662/1‑140 (MQ=255)
                                                   aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  7:641056/1‑140 (MQ=255)
                                                   aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  5:961311/1‑140 (MQ=255)
                                                   aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  1:980952/1‑140 (MQ=255)
                                                   aTAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  2:1066470/1‑140 (MQ=255)
                                                   aTAACGTGACGTCAGGCGAGAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTTAAt                                                                                      >  5:157207/1‑140 (MQ=255)
                                                     aaCGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAAt                                                                                      >  4:1207249/1‑138 (MQ=255)
                                                           aCGTAAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGCCTCTATCGCCGGGGTGTTCACGTAAATGTTTTTGc                                                                              >  6:438844/1‑140 (MQ=255)
                                                              ccAGGCTATACAAAACGCATTTAACCTGC‑CTACCTTTCTCGGCAAAACAAACATCTGCGTCATAATAAAGGCCCA‑CCCCGGGCA‑TTTATTTAAAGC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCAcc                                                                           <  2:435702/139‑1 (MQ=255)
                                                                  gCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCa                                                                         >  3:1199815/1‑138 (MQ=255)
                                                                  gCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAgg                                                                       >  2:1220462/1‑140 (MQ=255)
                                                                      tacaaaaaGCTTTTCTCCTGCGCTA‑ATTTCCCGGCATACCAAACATCAGCGGCATAAAAACCGCCCA‑CCCCCGGAA‑TTTTATAAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaa                                                                   <  6:1077483/137‑1 (MQ=255)
                                                                      aataaaacGCCTTTCACCGCCGCTA‑CTTTCTCGGCAAAACAAACATCCGCGGCATAAAAAACGCCCC‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaa                                                                   <  2:872427/137‑1 (MQ=255)
                                                                      aaGAAAAATCCTGTCTCCTGCGCTA‑GTTTCTCGTAATAAAAAACATCAGCGGAATATTAACCGGCCC‑GCCCGGTAA‑TTTTATACAATC‑CAACTACTCTCTCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGTCa                                                                     <  4:1302614/137‑1 (MQ=255)
                                                                        gAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCaaa                                                                   >  3:672139/1‑138 (MQ=255)
                                                                              cccTGTCTCCTGCCCTA‑CTTTCTCGCCATAACAAACACCCGCGCAATAAAAAACGTCCC‑CCCCCGGAA‑TTTTATTAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTc                                                             <  3:1100750/137‑1 (MQ=255)
                                                                                ttttCTCCTGCCATA‑CTTTCTGGCCAAAAAAAACACCCGCGGAAAAAAAAACGCCCA‑CCCCGCGCT‑TTTTATTAACCC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCg                                                           <  3:443618/137‑1 (MQ=255)
                                                                                 tttCTCATGCGATA‑CTTTATCGGCATAACAAACATCAGCGTCATAAAAACCGTCAA‑GCCCCGGAA‑TTTTATTAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCAt                                                        <  8:836318/140‑1 (MQ=255)
                                                                                   tctcCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTAACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTg                                                      >  1:1103077/1‑140 (MQ=255)
                                                                                     gccTCCGCAA‑ATTTCTCGGAAAAAAAAACATCAGCTGAAAAATAAACGTCCA‑CCCCCGGAT‑TTTTATTAAACC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                                    <  5:196371/139‑1 (MQ=255)
                                                                                       ttGCGCTA‑CTTTCTCGGAAAAACAAACATCAGCGGAATAATAAACGTCCA‑GCCCCTGCA‑TTTTATAAATAA‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTgcg                                                    <  3:986042/137‑1 (MQ=255)
                                                                                         gcccTA‑CTTTCTCGGCATAACAAACATCAGCGGCAAAATAACCGTCCC‑GCCCGGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGcc                                                  <  4:1176210/135‑1 (MQ=255)
                                                                                            cTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGt                                               >  4:438032/1‑138 (MQ=255)
                                                                                                cTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CACCGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTg                                          >  6:818633/1‑140 (MQ=255)
                                                                                                cTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTATATTAAATC‑CAACGACTCTATCGACGTGGTGTTCACGTAAATGTTTTTCCACACAGCCAAAATATTCAGCATTGCGCCCGTGAATg                                          >  1:643927/1‑140 (MQ=255)
                                                                                                   tcgcGGAATAAAAAACATCCGCGGCATAATAACCGCCCC‑GCCCGGGCA‑TTTTATTAAAAC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTa                                       <  1:480712/137‑1 (MQ=255)
                                                                                                     tcGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATg                                     >  2:211615/1‑140 (MQ=255)
                                                                                                                accaTCCGCGGCATAATAAACGTCCA‑GCCCCGGCT‑TTTTATTAAAGC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          <  2:354122/138‑1 (MQ=255)
                                                                                                                accaTCAGCGTCAAAAAACTCGCCCC‑GCCCGGCCT‑TTTTATAAAATC‑CCACGACTCTATCGACTGTGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          <  1:213898/138‑1 (MQ=255)
                                                                                                                aacaacAGCGGCAAAAAAAACGGCCC‑GCCCCGGCA‑TTTTATTAAAGC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCg                          <  2:70167/140‑1 (MQ=255)
                                                                                                                aacaTCAGCGGCATAATAAACGTCCC‑GCCCCGGCA‑TTTTATTAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                            <  4:63873/138‑1 (MQ=255)
                                                                                                                aacaTCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAAc                            <  4:54427/138‑1 (MQ=255)
                                                                                                                   aTCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCa                       >  6:1294857/1‑140 (MQ=255)
                                                                                                                     cAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGcc                       >  4:200285/1‑137 (MQ=255)
                                                                                                                      aGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCTCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACtt                    >  5:1210482/1‑140 (MQ=255)
                                                                                                                      aGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACtt                    >  5:877893/1‑140 (MQ=255)
                                                                                                                         gcaaaaataaCAGTCCA‑CCCCCGCAA‑TTTAATTAAAAC‑CAACGACGCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGa                 <  6:423557/135‑1 (MQ=255)
                                                                                                                          taataataAACTTCCA‑GACCCGTCA‑TTTTATTAAATC‑ACACGACGCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt                <  2:796472/138‑1 (MQ=255)
                                                                                                                          gaataataAACTTCCA‑GCCCCGGAA‑TTTTATTAATTC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt                <  6:877893/138‑1 (MQ=255)
                                                                                                                          gCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt                <  2:1259456/140‑1 (MQ=255)
                                                                                                                          gCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTATTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGTCACTTTg                  >  3:294609/1‑138 (MQ=255)
                                                                                                                          ccatcaaaaaCTTCCA‑GCCCCGGAA‑TTTTATTAAAAC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTGTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt                <  8:715830/133‑1 (MQ=255)
                                                                                                                          ccATAATAAACTCCCC‑CCCCCGGCA‑TTTAATTAAAAC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGAt                <  8:585694/139‑1 (MQ=255)
                                                                                                                           caaaataaACGTCAC‑GCCCCGGCA‑TTTTATAAAACC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  6:716083/137‑1 (MQ=255)
                                                                                                                           cATATTAAGCGTCCC‑GCCCCGCCA‑TTTTATAAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  7:1163309/140‑1 (MQ=255)
                                                                                                                           cATAATAACCGTCCA‑GCCCCTGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  6:462646/140‑1 (MQ=255)
                                                                                                                           cATAAAAAACGTCCC‑GCCACGGAA‑TTTTATTAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  6:711091/140‑1 (MQ=255)
                                                                                                                           ataaatacAGGTCCT‑GCCCGGGCT‑TTTTATTAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  6:1025257/137‑1 (MQ=255)
                                                                                                                           aataataAAGGTCCA‑CCCCCTCAT‑TTTTATTAAAAC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  7:489001/139‑1 (MQ=255)
                                                                                                                           aataataAACGTCCC‑GCCCCGGCA‑TTTTATTAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  6:973002/139‑1 (MQ=255)
                                                                                                                           aataataAACGCCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  2:765664/139‑1 (MQ=255)
                                                                                                                           aataaaaACCGCCCA‑CCCCCGGAA‑TTTTATAAAACC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  5:1020430/139‑1 (MQ=255)
                                                                                                                           aaaaataaACGTCCA‑GCCCC‑GTA‑TTTTTATAAATCACAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATc               <  1:1167376/137‑1 (MQ=255)
                                                                                                                              aataaCCGTCCC‑GCCCCGTCT‑TTTTATTAAAAC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCa              <  4:391548/138‑1 (MQ=255)
                                                                                                                              aataaACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt            <  2:1302189/140‑1 (MQ=255)
                                                                                                                              aataaACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt            <  8:920678/140‑1 (MQ=255)
                                                                                                                              aaaaaaCGTCCC‑GCCCCCGCT‑TTTTATTAATAC‑CAACGAATCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt            <  1:820697/137‑1 (MQ=255)
                                                                                                                              aaaaaaCGACCC‑CCCCCGCCA‑TTTTATTAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGt            <  5:976363/137‑1 (MQ=255)
                                                                                                                                 aaaCCTCCACGCCCGGTCA‑TTTTATAAATTC‑CAACGACTCTATCTACGGGGTGTTCACGTAAATGTTTTTTCACCCAGGCAAAATATTCCGCATTGCGCCCTTGTTTGGTATGAGATTTAACCGGCACTTTGATCAGTTc          <  8:177863/140‑1 (MQ=255)
                                                                                                                                  aaCGCCCA‑G‑CCCGGCATTTTTATTAAAT‑‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa       <  7:352845/140‑1 (MQ=255)
                                                                                                                                   ccccccA‑GCCCGGGCA‑TTTTATAAAATC‑CCACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa       <  8:1055265/136‑1 (MQ=255)
                                                                                                                                   aCGTCCA‑GCCCCGGCA‑TTTTATTAACTC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaaa       <  6:838312/140‑1 (MQ=255)
                                                                                                                                    cGTCCA‑GCCCCGGCA‑TTTTATTATATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTATTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGCTTGGTATGCGATTTAAGCTGCACTTTGATCAGTTCat        >  3:788837/1‑137 (MQ=255)
                                                                                                                                    cGTCCA‑GCCCCGCAT‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCaa        <  3:1062460/138‑1 (MQ=255)
                                                                                                                                     gTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGAGATTTAACCGGCACTTTGATAAGTTCAAATa     >  1:1267416/1‑140 (MQ=255)
                                                                                                                                        cA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATACAt  >  7:108637/1‑140 (MQ=255)
                                                                                                                                        cA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATACAt  >  5:701806/1‑140 (MQ=255)
                                                                                                                                        cA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCAGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATACAt  >  6:656749/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                             
TGTCGGCTTGCCCTGTTCGAAGGTTGCCCGCTCAAAGGCGGCATAATGGCGATAACGTGACGTCAGGCGATAGAAAAAGCCTTTCTCCTGCGCTA‑CTTTCTCGGCATAACAAACATCAGCGGCATAATAAACGTCCA‑GCCCCGGCA‑TTTTATTAAATC‑CAACGACTCTATCGACGGGGTGTTCACGTAAATGTTTTTGCACCCAGGCAAAATATTCCGCATTGCGCCCGTGATTGGTATGCGATTTAACCGGCACTTTGATCAGTTCAAATACAT  >  NZ_CP022686/2546264‑2546538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A