Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F13 I0 R1 203 11425.2 8793538 98.4% 8652841 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,572,721 A→C 12.1% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,572,7210AC12.1% 379.8 / 15.2 212F186C (TTT→TGT) CIW80_RS12885ROK family protein
Reads supporting (aligned to +/- strand):  ref base A (106/76);  new base C (2/28);  total (108/104)
Fisher's exact test for biased strand distribution p-value = 4.69e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTTGCTTGA  >  NZ_CP022686/2572582‑2572856
                                                                                                                                            |                                                                                                                                       
     gCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGAc                                                                                                                                    >  2:1130133/1‑140 (MQ=255)
           ggCGAAACCGTCCCACCTTATGATTGGCACAAGCATCACAACTTTTTGCCAGTGTGACAATCGCCGCATAATTGAGAATTTTACCATTCATCTGTTTAAAGCGTACAC‑TAAAACCGCTGCCGGAAACACACGACTCCACg                                                                                                                              <  2:1103904/140‑1 (MQ=255)
           ggCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACg                                                                                                                              >  5:210556/1‑140 (MQ=255)
                     ggCCACCTGCTGCATGGAACAAGCATCACCACGTTGGGCCAGGGTTAAAATCGCCGCATATGTGAGCAAGTAACCAGTCATCTGTTGATAGCGTTCAC‑TAAACCCGCTGCCGCAAACACACGACTCCACGCAGTTGTGtt                                                                                                                    <  5:955116/139‑1 (MQ=255)
                      gCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCAGCTTAATTGAGAAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGAGTCCACGCAGTTGTGttt                                                                                                                   >  8:279886/1‑140 (MQ=255)
                        cACCTGCTGCATGGCACAAGCATCACCACTTTGTGCCAGGGTGAAAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTTATACCGTTCAC‑TAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGc                                                                                                                 <  2:454376/140‑1 (MQ=255)
                        cACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGATGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGCCTCCACGCAGTTGTGttt                                                                                                                   >  3:591564/1‑138 (MQ=255)
                           gtgctgcTTGGCAAATGCATCACAACGTTGTCCCTGGGTGACAATCGCCGCAGATGTGAGCAATTTACAAGTCATTTGTTGATCGCGTTCAC‑TAAACCCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGc                                                                                                              <  6:110284/139‑1 (MQ=255)
                                   tGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCaa                                                                                                      >  7:339109/1‑140 (MQ=255)
                                      cacaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGCAGTGAGCAACTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAac                                                                                                     >  4:853899/1‑138 (MQ=255)
                                      cacaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactg                                                                                                   >  6:484901/1‑140 (MQ=255)
                                        caAGCATCACCACGTTGTGCCAGGGTGCCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGAGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactgac                                                                                                 >  6:800906/1‑140 (MQ=255)
                                        caAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactgac                                                                                                 >  2:258102/1‑140 (MQ=255)
                                        caAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactg                                                                                                   >  4:715312/1‑138 (MQ=255)
                                             aTCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactgactgc                                                                                              >  4:507259/1‑137 (MQ=255)
                                                accacGTTGTGCCAGGGTGACACTCGCCGCCGCAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGGTCAC‑TAACCCCGCTGCCGGAAACAAACGACTCCCCGCAGTTGTGTTTGCCGCAATATCAACTGCCTGATGGAc                                                                                         >  6:718827/1‑140 (MQ=255)
                                                accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCATGTTACCAGTCTTCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATgg                                                                                           <  3:868689/138‑1 (MQ=255)
                                                accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                         >  1:560647/1‑140 (MQ=255)
                                                  cacGTTGTGCCAGGGTTACAATCGCCGCAGAAGTGATCAAGTTACCAGTCATCTGTTGATATCTTTCAA‑TAAAACCGCTGCCTGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCa                                                                                       <  6:315130/140‑1 (MQ=255)
                                                  cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCa                                                                                       <  8:151729/140‑1 (MQ=255)
                                                  cacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGGTGTGATTGCCGCAATAGCAACAGACTGATGGAc                                                                                         >  3:27241/1‑138 (MQ=255)
                                                  cacGTTGGGCCAGGGTGACAATCGCCGCAGACGTGAGCAAGTTACCATGCATCTGTTGATCGCGTTCAC‑AAAACCCGCTGCGGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCa                                                                                       <  5:859754/140‑1 (MQ=255)
                                                       tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGATTGATGGACCCTCTTc                                                                                  >  7:218127/1‑140 (MQ=255)
                                                       tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                                    >  3:1105830/1‑138 (MQ=255)
                                                       tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                  <  7:304975/140‑1 (MQ=255)
                                                       tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                  >  8:206948/1‑140 (MQ=255)
                                                       tgtgCCAGGGTGACAATCGCCGAAGACGTTACCAAGTAACCAGGCATCTGTTTATCGCGTACAC‑AAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                  <  2:205456/140‑1 (MQ=255)
                                                       tgtgCCAGGGGGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTGGTGTTTGCCGCAATAGCAACTGAATGATGGCCCATCtac                                                                                  >  7:998820/1‑138 (MQ=255)
                                                       ggtgCCTGGTTGACATTCGCCGCAGAAGTGAGCAAGTTACCAGGCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                                  <  2:701122/139‑1 (MQ=255)
                                                         tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGCCTGATGGACCATCTTACt                                                                                >  5:1024875/1‑140 (MQ=255)
                                                         tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                                >  1:241459/1‑140 (MQ=255)
                                                         tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                                >  8:318287/1‑140 (MQ=255)
                                                         tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                                >  7:100847/1‑140 (MQ=255)
                                                         tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGATGTGTTAGCAGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                                >  7:100851/1‑140 (MQ=255)
                                                          gCCAGGGGGACACTCGCCGAAGACGTGAGCAAGTTACCAGGCATCTGTTGATTGCGTTCAC‑TAAACCGGCTCCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                               <  2:838148/140‑1 (MQ=255)
                                                          cacAGGGTCAAAATCGCCGCACAAGGGACCCAGTAACCAGTACACTTTTTATTGCGTGCAA‑TAAACCCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                               <  7:919433/138‑1 (MQ=255)
                                                            ctgggTTACAATCGCCTCATAAGGTACCATGTTACCAGTAATCTTTTTATAGCGTCAAA‑TAAAACCTCTGCCTTAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                             <  8:576192/138‑1 (MQ=255)
                                                            ctgggTGACTCTGGCCGCAGAAGGGAGCAAGTTACCAGTCATCTGTTGATCGGGTTCAC‑AAAAACCGCGGCCGGATACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                             <  2:439855/138‑1 (MQ=255)
                                                            cAGGGTTACAATCGCCGAATAAGTGCCAATTTTACACGGATTTTGTTTATCGCGTCCAA‑TAAACCCTCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                               <  3:1016932/138‑1 (MQ=255)
                                                            cAGGGTTAAATTCGCCGCAGACGTTAGCAAGTTACCAGTCATCTTTTGATACCGTTCAA‑TAAAACCGCTTCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                             <  8:673715/140‑1 (MQ=255)
                                                            cAGGGTGACAAGCGCCGCAGATGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑AAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                             <  8:626919/140‑1 (MQ=255)
                                                            cAGGGTGAAAATCGCCTCAGATGTGAGAATTTTACCAGTCATCTGTTTATAGCGTACAA‑TAAAACCGCGGCCGGAAAAACACGACTCCACGCAGTTGTGTTTGCCTCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                               <  3:962112/138‑1 (MQ=255)
                                                               ggTGACATGCGCCGCATTTGTGAGCATGTTACCAGCCATCTGTTTATAGCGTTCAC‑TAAACCCGCTGCCGGAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                          <  8:808835/140‑1 (MQ=255)
                                                                gTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAACCGCCTCCACGCAGTTGTGTTTGCCGCAATAGCAACGGACTGCTGGACCATCTTCCGGCTCCgg                                                                         >  5:632158/1‑139 (MQ=255)
                                                                 ttaCAATCGCCGCACAAGTCAGCAAGTTACCTGTCTTCTGTTTATAGCGTTCAC‑TAAAACCGCGGCCGTAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                          <  4:168799/136‑1 (MQ=255)
                                                                 tGACAATCGCCGCAGATGTGAGCATGTTCCCCGTCATGTGTTGATAGGGTCCAC‑TAAACCCGCTGCCGCAACAACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                        <  1:302280/140‑1 (MQ=255)
                                                                   aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                      >  1:44033/1‑140 (MQ=255)
                                                                   aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGc                                                                                                              >  1:725374/1‑100 (MQ=255)
                                                                   aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                      >  7:390376/1‑140 (MQ=255)
                                                                   aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                      >  6:246584/1‑140 (MQ=255)
                                                                     aaTCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                                    <  3:46938/124‑1 (MQ=255)
                                                                     aaTCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                      >  3:554421/1‑138 (MQ=255)
                                                                     aaTCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                      >  3:358282/1‑138 (MQ=255)
                                                                     aaTCCCCGCTCAAGGGAGCAAGTTCCCAGTCATCTTTTGATAGCGTCCAC‑TAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                      <  4:539688/138‑1 (MQ=255)
                                                                         gccgcAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGtt                                                                                                                    <  2:48574/88‑1 (MQ=255)
                                                                         cccgcAGAAGTGAGCATTTTACACGTAATCTGTTTATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCt                                                                  <  4:509793/137‑1 (MQ=255)
                                                                          ccgcAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGc                                                               >  2:789235/1‑140 (MQ=255)
                                                                          ccgcAGAAGTGAGCAAGTAACCCGTAAGCTGTTGATAGCTTTCAC‑TAAACCCGCTGCCGCAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGc                                                               <  1:495650/140‑1 (MQ=255)
                                                                            gcttttttGAGAATGTTACCAGGCATGTGTTGTTAGGGTACTA‑ATAACCCGCTGCGGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCaa                                                             <  7:806779/133‑1 (MQ=255)
                                                                            gcAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGc                                                               >  4:1040655/1‑138 (MQ=255)
                                                                             cAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGCCCATCTTCCTGCTCCGTATAGCCTGGCAAc                                                            >  5:838746/1‑140 (MQ=255)
                                                                                     aGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGCCTGCTGGACCCTCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                                    >  1:1004190/1‑140 (MQ=255)
                                                                                      ccAAGTATCCAGACATGTGTTTCTAGCG‑TCACATAAACCCGCGGCCGTAAACACACGACTCCACGAAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTTATATGa                                                   <  7:32208/139‑1 (MQ=255)
                                                                                        aaGTTACCAGTCACCTGTTGAAAGCCTTCAC‑AAAAACCCCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGa                                                   <  4:572536/138‑1 (MQ=255)
                                                                                           taacgaGTCATGTGTTGATATCTTTCAC‑TAAAACGGCTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTTCCGCAATAGCAACTGACTGATTGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCa                                              <  7:711646/135‑1 (MQ=255)
                                                                                            tACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAACCGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCCTCTTCCTCCTCCGTATAGCCTGGCAACGTGATATGACCGCAt                                             >  2:175979/1‑140 (MQ=255)
                                                                                            tACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGc                                               >  4:371924/1‑138 (MQ=255)
                                                                                            tACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCGTCCTGCTCCGTATAGCCTGGCATCGTGATATGACCGc                                               >  4:272905/1‑138 (MQ=255)
                                                                                             aCCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCATCATGCTCCGTATAGCCTGGCAACGTGATATGACCGCAtt                                            >  6:644350/1‑140 (MQ=255)
                                                                                              ccAGTCATCTTTTGATAGCGTTAAA‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTc                                           <  1:475089/140‑1 (MQ=255)
                                                                                              ccAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAACATCTTCCTGATCCGTATAGCCTGGCAACGTGATATGACCGctt                                             >  4:341754/1‑136 (MQ=255)
                                                                                                aGTCATCTTTTTATAGCTTTCAC‑TAAACCCGCTGCCCGAAACAAACGACTCCAAGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTc                                           <  4:665655/138‑1 (MQ=255)
                                                                                                aGTCATCTGTTTATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATGTGACCGCATTc                                           <  4:900885/138‑1 (MQ=255)
                                                                                                  tCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgcc                                       >  5:977622/1‑139 (MQ=255)
                                                                                                  tCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgca                                       >  5:5393/1‑140 (MQ=255)
                                                                                                  tCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgca                                       >  1:79833/1‑140 (MQ=255)
                                                                                                    aTCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGCCCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgccgc                                     >  6:1004901/1‑140 (MQ=255)
                                                                                                    aTCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgcagc                                     >  7:1046096/1‑140 (MQ=255)
                                                                                                      cTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCAt                                   <  5:330244/140‑1 (MQ=255)
                                                                                                      cTGTTGATACCGGACAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCAt                                   <  6:877154/140‑1 (MQ=255)
                                                                                                           taTAGCGTTCAC‑TAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTccc                              <  1:139669/139‑1 (MQ=255)
                                                                                                            aTAGAGTTCAC‑TTAAACCTCTACCGGAAAAAAACGACTCCACGCAGTTGTGATTGCCGCAATAGAAACTGACTGATGGACCATCTTCCTGCTCCTTATAGCCTTGCAACGTGATATGACAGCATTCGGCAGCATTTcccc                             >  8:72805/1‑140 (MQ=255)
                                                                                                              aGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg                           >  1:1028226/1‑140 (MQ=255)
                                                                                                              aGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg                           >  1:110236/1‑140 (MQ=255)
                                                                                                               gCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                          <  1:626279/140‑1 (MQ=255)
                                                                                                               gCGTTCAC‑TAAAACCGCTCCCGGAAACAAACGACTCCTCGCAGTTGTGTTTGCCGCAATAGCAACTGAATGATTGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATTACCGCATTCGGCAGCATTTCCCCTgg                          <  6:362892/140‑1 (MQ=255)
                                                                                                               gCGTTCAC‑TAAAACCGCGGCCGGAACCAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATTGACCATCTTCCTGCTCCTTATAGCCTGGCAACGTGATATGACAGCATTCGGCAGCATTTCCCCAgg                          <  8:1040904/140‑1 (MQ=255)
                                                                                                               ccGTTCCA‑TAAAACCGCTCCCCCAAAAACACGACTCCACGCAGTTGTGGTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                          <  5:231264/139‑1 (MQ=255)
                                                                                                                cGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg                           >  4:1067576/1‑138 (MQ=255)
                                                                                                                 gTTCAC‑TAAAACCGCTGCGGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcc                        <  2:627333/140‑1 (MQ=255)
                                                                                                                 gTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcc                        >  5:294158/1‑140 (MQ=255)
                                                                                                                   tCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc                      >  8:893855/1‑140 (MQ=255)
                                                                                                                          aacccGCAGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACAGCATTCGGAAGCATTTCCCCAGGCCCCtataca                <  5:601390/137‑3 (MQ=255)
                                                                                                                           aaCCGCTGCCGGAAACAAACGACTCCAAGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCCTCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAAc               >  1:946008/1‑140 (MQ=255)
                                                                                                                                cttccGGAAAATAACTACTACACGCAGTTCTGGTTGCCGAAATAGCAACTGACTTATGGACCATATTCCTTCTCGGTATAGCCTGGAAACGTTATATTACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGt            <  4:1007173/135‑1 (MQ=255)
                                                                                                                                cggCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg          <  5:8042/138‑1 (MQ=255)
                                                                                                                                cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCAGTTCCCCAGGCCCCTATAAACGGTCg          >  7:537256/1‑140 (MQ=255)
                                                                                                                                cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCAGTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg          >  6:297889/1‑140 (MQ=255)
                                                                                                                                 gcccGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGTCCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGt         <  1:386432/138‑1 (MQ=255)
                                                                                                                                  gCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                               >  4:841826/1‑69 (MQ=255)
                                                                                                                                            |                                                                                                                                       
GGCAAGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAC‑TAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTTGCTTGA  >  NZ_CP022686/2572582‑2572856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A