Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,547,110 G→C 15.0% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,547,1100GC15.0% 119.3 / 3.0 79intergenic (+22/‑12)CIW80_RS07710/CIW80_RS07715tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (41/24);  new base C (9/4);  total (51/28)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCT  >  NZ_CP022686/1546971‑1547239
                                                                                                                                            |                                                                                                                                 
ctcAACCAATTAAAATTTGCAGTAGTAAACCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAg                                                                                                                                   <  7:700564/138‑1 (MQ=17)
ctcAACCAATAATACTTTGATGAATACTAGCAGTCCGGGGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAg                                                                                                                                   <  6:297040/138‑1 (MQ=14)
cGCAACCAATTAAATTTTGATGAATTAAAGCAGTACTGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGTGCTCATAACCCGAAGGTCGTCGGTTCATATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAg                                                                                                                                   <  8:332716/140‑1 (MQ=17)
cGCAACCAATTAAAATTTTAAGTATTCTATAAGTCCTGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATTACCCGAAGGTCGTCGGTTCAACTCCGGCCACCGCAACCTAT‑CAAAATTTGATGATATACAg                                                                                                                                   <  2:472276/140‑1 (MQ=14)
cGCAACCAATTAAAATTTGATGAAGTTACGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAg                                                                                                                                   <  6:1027467/140‑1 (MQ=255)
cGCAACCAATAAAAATTTGTAGAAGTAACGCCGTCCGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAg                                                                                                                                   <  7:848436/140‑1 (MQ=17)
  cAACCAATTAAAATGTGATGACGTAACGCATTACGGGGACTCGGGGTGGAGCAGCATGGTATCTCGTCGGGCGCATAACCCGAAGGTCGTCGGTGCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAg                                                                                                                                   <  3:37622/138‑1 (MQ=17)
             attttGATGAAGTAACGCCGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCGCGTCGGGCTCATAACCCGAAGGTCGGCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGAcg                                                                                                                      <  7:1230478/138‑1 (MQ=17)
             acattGATGAATTTACGCACTCCCGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGa                                                                                                                        <  4:420507/135‑1 (MQ=14)
             aaTTTGATGAAGTACTGCCTTACTTGGCCGCGGGGTGGAGCAGCATTGTAGCTCGTCGGGCTGATAACCCGAAGGTCGTCGGTTCAAATCAGGCCCCCGCAACCAAT‑CAAAATTTGATGAACTAAAGCAGTACGGTGAcg                                                                                                                      <  1:1260355/140‑1 (MQ=11)
             aaTTTGATGAAGTAAAGCATTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTAATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGa                                                                                                                        <  3:28379/138‑1 (MQ=255)
               tttGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTAGATGAAAAAAAGCAGTACGGTGActcg                                                                                                                    >  7:407920/1‑140 (MQ=255)
               tttGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCGCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAATTTAGATGATAAAAACCAGTACGGTGAcgcg                                                                                                                    >  7:1184566/1‑140 (MQ=17)
                  gatgaAGAATCGCAGTCCGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGATGAAATAAAGCAGTACGGTGACGCgggg                                                                                                                 <  7:488722/140‑1 (MQ=14)
                       aGTATAGCATTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                            <  5:1026907/140‑1 (MQ=255)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGATAAAAAGCAGTACGGTGCCTCGGGGTGGCGCTGGATGGTa                                                                                                  >  7:1123556/1‑140 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTAATCAAATAAAGCAGAACGGTGCCGCGGGGTGTCGCAGCATGGTa                                                                                                  >  1:67993/1‑140 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTAAAATCCGGCCCCCGCACCCAAT‑CAAATTTTGATGAAATAAACCACTACGGTGACGCGGGTTGGGGCAGACTGGtt                                                                                                  >  1:804882/1‑139 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGACGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAAAAAAGCAGAACGGTGCCGCTGGGTGGAGCTGGCTGGTa                                                                                                  >  1:389068/1‑140 (MQ=14)
                                   cGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CTAATTGAGAGGAAAGAAACCACTACGGTGCCTGGGGGTGGAGCAGCGTGGtt                                                                                                  >  3:464323/1‑137 (MQ=14)
                                      tGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGCAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTTAGGAAAGAAAGCAGAACGCTGAAGAGGGGTGGCGCAGACTTGTatatcc                                                                                             >  5:924214/1‑135 (MQ=11)
                                       gccccgGGGTGGAGCATCATTGAAGCTCGTCGGGCACATACACAGAATGTCTTCGTTCCAACTCCGCACCCCGACACCAAT‑AAAAAATTGATGAATAAACGCACTCCGGGGACGCGGGGTGGAGCAGCCTGGTAGCtcgt                                                                                            <  6:1049947/136‑1 (MQ=2)
                                       caCGCGGTGTGGTGCAGCATGGTTCCTCGTCTGGATCTTTACCCGAAGGTCGTCGGGGCAAATCCGTCCCAGGCAACGATT‑AAAAATTTTATGACTTCAGGCAGTACGGTGACGCGGGGTGGAGCAGCTTGGTAGCtcgt                                                                                            <  2:1062628/139‑1 (MQ=2)
                                        aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑GAAAATTTGATGAAAAAAAGCAGTACGGTGCAGCGGGGTGGAGCAGCATGGTAGAtcgtc                                                                                           >  7:38400/1‑140 (MQ=11)
                                        aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAAATTGATGAAATAAACCAGTACGCTGACGCGGGGTGGCGCTGCCTGGTAGCtgg                                                                                             >  3:270708/1‑136 (MQ=14)
                                        aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAATTTTGTTGAGAAAAACCAGTACGGTGCCCCGGGGTGGCGGAGAATGGTAGAtcggc                                                                                           >  6:565496/1‑138 (MQ=11)
                                         ctcgcggTGGATCAGCCTGGTTGCTAGTCGGTCTCATAACCCGAAGGGCTTCGGTTCAAATCCGTCCCCCGCAACCAAT‑AAAAATTACATGAAATCTAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgt                                                                                            <  4:1103495/133‑1 (MQ=11)
                                         cgcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGGCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTCCGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                          <  5:600405/140‑1 (MQ=255)
                                         cgcgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACGAAT‑CAAAATTAGATGAATTAAAGCACTACCGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgt                                                                                            <  3:185003/138‑1 (MQ=11)
                                          gcgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGTTGATAAAAAGCAGTACGGTGACGAGGGGTGGAGCAGACTGGTAggtagtg                                                                                           >  3:876426/1‑132 (MQ=11)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCgggg                                                                                                                 <  5:370889/115‑1 (MQ=39)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCgggg                                                                                                                 >  6:370889/1‑115 (MQ=39)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGATGATATAAAGCAGTACGGTGACGCGGGGTGGAGCTGCCTTGTAGATCGTCggg                                                                                        >  1:859520/1‑140 (MQ=11)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGATGAAAGAAAGCACAACGGTGACGAGGGGTGGCGCTGGCTGGTAGCTCGTgggg                                                                                        >  7:724795/1‑140 (MQ=11)
                                             gggTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGAGGAAAAAAACCAGTAAGGTGCAGAGGGGTGGAGCTGCGTGGGATCTCGTCggg                                                                                        >  4:598484/1‑138 (MQ=11)
                                               gTGGAGCAGCATGGTTGATCTTCTGGCTCAAAACCCGAAGGTCGTCGGTTCAAAACAGGCCCCCGCAAACAAT‑AAAAATTTGTTTTAATAATCCATTACTGTGACGCGGGGGGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                    <  6:654283/140‑1 (MQ=11)
                                               gTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGa                                                                                                                        >  3:56349/1‑104 (MQ=40)
                                               gTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGa                                                                                                                        <  4:56349/104‑1 (MQ=40)
                                                tGGAGCAGCATGGTAGCTCGTCGGGCACATAACCCGAAGGTCGACGGTTCAAAACCGGCCCCCGCAACCAAT‑CAAAAATAGATGAAATAAAGCAGTAAGGTGACGCTGGGTGGAGCAGCCTGCTTGCTAGTCGGGCTAAt                                                                                   >  7:153049/1‑140 (MQ=14)
                                                gggAGCAGCCTGGTCTCTCGGCGGTCTCATAACCCGAATGGCGTCGGGGCTAAAACTGCCGCCGGAACCAAT‑AAAAATGTGATGAAAAAACCCCCTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt                                                                                   <  2:966125/139‑1 (MQ=2)
                                                        caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTTATCATATAAAGCAGAACGGTGACGCGGGGTGGAGCAGCCTGGTTGCTAGTCGGGCTCTTACCCCGaa                                                                           >  5:641384/1‑140 (MQ=11)
                                                        caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGATGATAAAAAGCAGTACGGTGAAGCGGGGTGGAGCAGCCTGGTTGCTAGTCGGGCGGTTAACCCGaa                                                                           >  1:708269/1‑140 (MQ=11)
                                                             tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGATAAAAAGCAGTACGGTGCCTAGGTGTGGAGCATACTGGTATCTAGACGGGCTCATAACCCGAACgtcg                                                                      >  7:835247/1‑140 (MQ=11)
                                                             tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAAAAAAGCACTACGCTGACGCTGGGTGGCGCAGCATGGTAGATGGGCGGGCTCTTACCCCGAAGgtcg                                                                      >  8:919287/1‑140 (MQ=11)
                                                             tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAAATTGATGAAAAAAAGCACTAAGGTGACGCGGGGTTGAGCAGCCTTGCAGCTAGTCGGGCGCATAACCAGAAGgtcg                                                                      >  2:562541/1‑140 (MQ=11)
                                                              tgCTCGGCTGTCTCAAAACCCGAAGGTCGTCGGTGCTAATCCGGCCCCCGCAACCAAT‑CAAATTTAGTTGAAATTACGCCGTACTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                     <  1:158041/139‑1 (MQ=11)
                                                              tgCTCGGCGGGCTCATAACCCGACGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGATGAAAAAACGCACTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                     <  6:145355/139‑1 (MQ=11)
                                                              cgCTCGTCGGGCTCATAACCCGAAGGGCGGCGGTTCAAAACCTGCCCCCGCCACGAAT‑CAAAATTTGATGAATATAACCCGTCCGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                     <  7:375840/139‑1 (MQ=11)
                                                              aGCTCGGCGGGCTCATAACCCGAAGGGCGGCGGTTCAAATCCGGACCCCGGAACCAAT‑CAAAAATTCGAGAAATAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                     <  1:208042/140‑1 (MQ=11)
                                                               gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGAGGAAATAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTAGCTCGGCGGGCTCATAACCCGAAggtggtg                                                                    >  5:23921/1‑139 (MQ=255)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAATTTTGATGAAATAAAGCAGTACGGTGCCGCGGGGTGGAGCAGCATGGTAGCTAGTCGGGCTCATAACCCGAAGGTCGTCGGt                                                                 >  7:342704/1‑140 (MQ=255)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGATATAAAGCCGAACTGTGACGCGGGGTGGAGCAGCATGGGAGCTCGACGGGCACATAACCCGAAGGGCGTCGGt                                                                 >  1:350636/1‑140 (MQ=11)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                            <  6:527944/97‑1 (MQ=37)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                            >  5:527944/1‑97 (MQ=37)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAAGAAAGCACTACGGTGACGTGGGGTGGAGCAGCCGGGATGCTCGACGGGCTCATAACCAGAAGGTCGTCGGt                                                                 >  2:848098/1‑140 (MQ=11)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGATGAAAAAAACCCGTACGGTGCCGCGGGGTGGCGCAGCCTGGTTGATCGTCGGGCGCTTAACCAGACGGTCGTCGGt                                                                 >  6:1290607/1‑140 (MQ=11)
                                                                   tgcgctcTCATAAGCCGAATGTCGTCGGTTCCTAACCGGCCCCGGCAACCAATACTACATTT‑CTGAATTCACGCAGTCCAGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCgg                                                                  <  4:988288/132‑1 (MQ=2)
                                                                    tcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGATATAAAGCCGTACGGTGCCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCTTAACCGGACGGTCGTCggg                                                                 >  3:510884/1‑137 (MQ=11)
                                                                    tcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGa                                                                                                                        >  3:1076626/1‑83 (MQ=38)
                                                                    tcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGa                                                                                                                        <  4:1076626/83‑1 (MQ=38)
                                                                    tcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACTCGGGGTGGAGCAGCCTGGTTGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTc                                                               >  8:65892/1‑140 (MQ=255)
                                                                            aTAAGCCGAAGGGCGTCGGTGCAAATCAGGCCCCCGCAACGAAT‑CACATTTTGATGAATTAATGCAGTCCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATcc                                                         <  3:1131197/138‑1 (MQ=11)
                                                                             tAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CTAAATTTGATGAAAAAAAGCAGTACGGTGCCGCGGGGTTGAGCAGCGTGGTAGATCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAACCGGc                                                      >  7:512884/1‑140 (MQ=11)
                                                                               aCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGc                                                      >  3:454635/1‑138 (MQ=37)
                                                                                    aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGTTGAAATAAAGCAGTACGGTGCCGCGGGGTGGAGCATCCTGGTAGCTAGTCGTGCAAATAACCCGAAGGTCGAGGGTTCAAAACCGGCACCCGCa                                               >  8:127073/1‑140 (MQ=11)
                                                                                    aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAg                                                                                                 >  7:844388/1‑90 (MQ=255)
                                                                                    aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAg                                                                                                 <  8:844388/90‑1 (MQ=255)
                                                                                      ggTCGTCGTATCAAATCCGTCCCCCGCAACAAAT‑CAAAATTTTATAATTTAATGGCGTCCGGTGACGCGTTTTGGAGAAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                             <  5:597115/140‑1 (MQ=11)
                                                                                      ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAAAAAAGCAGTACGGTGACGCGGGGTGGAGCAGACTGGTAGATCGACGGGCTCATAACCAGAAGGTGGAGGGTTGACATGCGGACCCCGCa                                               >  4:979853/1‑138 (MQ=11)
                                                                                      ggTCGTCGGTTCAAAACCCGCCCCCGCAACCAAA‑AACACTGTGCTGAAATAAACCCGTCCTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                             <  7:1046475/140‑1 (MQ=11)
                                                                                          gtcgGTTCAAATCCGGCCCCCGCAACCAAT‑AAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCGCATAACCAGAAGGTCGTCGGTTCAAATACGGCCCACccaaccaaa                                         >  1:483961/1‑139 (MQ=11)
                                                                                             ggTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATAGGGCCCAAGCAACAAAtt                                        >  3:1107274/1‑138 (MQ=11)
                                                                                                    aTCCGGCCCCCGCAACCAAT‑GAAAATTTGATGAAATAAAGCAGTAAGGTGACGCGGGGTGGAGCATCCTGGTAGCTAGTCGGGCTCATCACCCGAAGGTGGTGGGTTCACATACGGCCCCCGCGACCAATTTATTAGAAt                               >  2:1350728/1‑140 (MQ=14)
                                                                                                    aTCCGGCCCCCGCAACCAAT‑CAAAATTTGATCATATAAAGCAGTACGGTGCCGCTGGGTGGCGCAGCCTGCTAGATAGACGGGCGCATACCCTGAAGGTCGTCGGTGCAAATCCGGCACCACCGACCAATTTAATAGaaa                               >  2:755612/1‑139 (MQ=9)
                                                                                                         gCCCCCGCAACCAAT‑TAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                    <  7:1258082/82‑1 (MQ=18)
                                                                                                         gCCCCCGCAACCAAT‑CAATATTAGAGCAAATAAACCAGTAAGGTGCCGCGGGGTGGAGCAGGCTGGTAGCTAGTCGGGCGCATACCCCGTCTGTCGTCGGTTCGACTAAGGCCACCGCCACCAAATAATTAGAAGGcagc                          >  6:857175/1‑137 (MQ=255)
                                                                                                         gCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGa                          >  1:392792/1‑140 (MQ=255)
                                                                                                         gCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAACCAGTACGGTGACGCGGGGTGGAGCAGACTGGTAGCTCGTCGTGCTCATAACCAGAAGGTCGTCGCTTCAAATCCGGCCCCCCCACACAATTTAATACAAAGCTGa                          >  7:563109/1‑140 (MQ=14)
                                                                                                         gCCCCCGCAACCAAT‑CAAAATTTGATGAAAGAAAGCGGTACGGTGCCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCGATTAACCCGAAGGTCCTCGGTTCCAATCAGGCCCCCGCCAACAATTTATTAGAATGCTGa                          >  7:495392/1‑140 (MQ=21)
                                                                                                         gCCCCCGCAACCAAT‑CAAAATTTGATGAAAAAAAGCAGTACGGTGCCTCGGGGTGGAGCAGCCTTGTAGCTCGTCGGGCTCATAACCCTAAGGTCGACGGTTCTAATACGGCCCCCCCAAACAATTTATTAGAATGCTGa                          >  7:1216910/1‑140 (MQ=17)
                                                                                                           ccccGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCTTAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCGACCAATTTATTAGAATGCTGa                          >  3:685397/1‑138 (MQ=255)
                                                                                                              cGCAACCAAT‑CAAAATTTGATGAAATAAATAAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCACATAACCAGAAGGTCGTCGGTTCAAATACGGCCCCCGCAACCAATTTATTAGAATGCTGACaaaa                     >  5:382406/1‑139 (MQ=17)
                                                                                                              cGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGCTAGCTCGTCGGGCTCATAACCCGAAGGTCCTCGGTTCAAATCCGGCACCCGCAACCAATTTATTAGAATTCTGACAAAc                     >  7:770306/1‑140 (MQ=14)
                                                                                                                    cAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGTGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGTCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCaaa               <  7:631296/140‑1 (MQ=255)
                                                                                                                      aT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGTGCACATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATTAGAAAGCTGACAAACCGCAAAgc             >  6:861809/1‑140 (MQ=17)
                                                                                                                                  atgaAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTATAATGCTGACAAACCGCAAAGCTGCTATTGGCt  >  5:66449/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                 
CGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT‑CAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCT  >  NZ_CP022686/1546971‑1547239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A