Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F36 I0 R1
|
179 |
8076.5 |
8499890 |
98.3% |
8355391 |
135.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NZ_CP022686 |
1,547,110 |
G→C |
24.0% |
intergenic (+22/‑12) |
CIW80_RS07710 → / → CIW80_RS07715 |
tRNA‑Met/tRNA‑Met |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP022686 | 1,547,110 | 0 | G | C | 24.0%
| 83.2
/ 7.5
| 59 | intergenic (+22/‑12) | CIW80_RS07710/CIW80_RS07715 | tRNA‑Met/tRNA‑Met |
| Reads supporting (aligned to +/- strand): ref base G (30/13); new base C (11/3); total (42/17) |
| Fisher's exact test for biased strand distribution p-value = 7.35e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCT > NZ_CP022686/1546971‑1547239
|
cGCAACGAATTATAAATTTATGAAGTAAACCAGTCCTGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg < 1:1069010/140‑1 (MQ=17)
cGCAACCAATTATAATTTTATGAATTATAGCAGTACTGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg < 4:706484/140‑1 (MQ=17)
cGCAACCAATTATAATTTGATGTAGTTACGCACTACGGTGACGCGGGGTGGAGCAGCATGGAAGCTCGGCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg < 1:527755/140‑1 (MQ=17)
cGCAACCAATTAAATATGTCTGAAGTATCGCACTCCGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg < 1:1092880/140‑1 (MQ=14)
aaTTAAAATTTGATGAAGTTAAGCAGTACTCGGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGGCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACg < 4:796891/140‑1 (MQ=17)
aTTAAAATTTGATGAAGTAAATCAGTACGGTGAAGCGGGGTGGAGCATAATGGTAGCTAGTCGGGCACATAACAAGAAGGTCGTCGTTTCAAATCAGGCCAACGCAACCAATAAAAATAAAATGAAAAAAAACACTaagt > 2:1036479/1‑137 (MQ=255)
aTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAACTCCGGCCACCGCAACCAATAAATAATAGATGAAAAAAACCAGTAAgg > 2:1050844/1‑140 (MQ=14)
aTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAAATATATGAAATAAACCAGTACgg > 5:436714/1‑140 (MQ=17)
atttttATGAAGTTAAGCATTACGGTGACGCGTGGTGGAGCAGCTTGGTATCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCATTACGGTGAcg < 3:500432/138‑1 (MQ=14)
attttGTTGAATTATAGCATTCCGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGTCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg < 4:1073354/138‑1 (MQ=14)
aacttcttgaAGTATCCCAGTACGCGGACGCGGGGTGGAGCAGCAAGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg < 4:1035549/133‑1 (MQ=14)
tttGAAGAAGTCATTCAGTACGGTGACGCGGGGTGGAGCAGGATGGTAGCTCTTCGGGATCATAAGCCGAAGGTCGTCGGTTCTAATCCGGCGCCCGAAACCAATAAAAATTTTATGAAATAAATCATTACGGTGAcgcg < 6:872821/140‑1 (MQ=17)
gtcgaAGTACAGCACTACTGTGACGCGGTGGGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAACTTTGATGAAATAAAGCATTACGGTGACGCgggg < 6:782427/137‑1 (MQ=14)
gTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATCAATATTTGATGTAAGAAACCAGTAAGCTGCCGCGGGGTGGcgcagaattgg > 4:26621/1‑134 (MQ=14)
tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAATTTTGATCAAAAAAAGCACTACGGTGACGGGGGGTTGAGCAGCTTTGTa > 2:780677/1‑140 (MQ=14)
tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGACCCCGCAACCAATAAAAATTTTATCAAAGAAACCAGTACGGTGCCGCGGGGTGGCGCAGCGTGGtg > 2:307068/1‑139 (MQ=14)
cGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAAAAAAGCAGAACGGTGACGGCGGGAGGAGCAGACTgctt > 7:744861/1‑135 (MQ=14)
cGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAAACCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTAAATTTTATGATATAAACCAGAACGGTGCCTCTGGGTGGCGCAGCCTGGtt > 7:474400/1‑137 (MQ=14)
aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTTTTGATAGAAAGCCGAACGGTGACTCTGGGTGGCGGATGCTGGTatgtactc > 4:271711/1‑133 (MQ=11)
aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAATTTATATGATAAAAACCACTACGGTGACGATGGGTGGCGCATAATGGTAGAtagtc > 3:796194/1‑140 (MQ=11)
aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGATAAAAAGCAGAACGGTGACGGGGGGTGGAGCAGCATGGTAtctcttg > 3:711397/1‑137 (MQ=11)
aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAAGAAAGCAGTACGCTGACTCGGGGTGGAGCTGGATGGTAGAtcgtc > 4:968235/1‑140 (MQ=11)
gcgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTTATGAAAAAAAGCAGTACGGTGCCGCGGGGTGGAGCAGCCTGGTAGCtcgtc > 7:1004751/1‑138 (MQ=255)
gcgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATATTTGAGGAAAGAAACCACAACGGTGACGAGGGGTGGCGCAGCATTGTATCtcgac > 7:1004747/1‑136 (MQ=11)
cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAATATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTTGCTGGTCggg > 3:1036822/1‑140 (MQ=255)
cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAAGAAACCAGTACGCTGCAGCGGGGTTGAGCTGCCTGGTAGGtggtcgtg > 3:629643/1‑138 (MQ=11)
cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAAATTGTTGATATAAACCGCAACGGTGCCGATGGGTTGAGGAGGCTTGTAGCtaaggggg > 6:219190/1‑133 (MQ=11)
ggcagcagcCAGGGAGCGCGGCGGGAACATACCCCGAAGGGGGCCGGTTCAACTCCCGCCCCCGCAACCAATCAAACATTCCTGAAATCACCCAGTCCCGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCAt < 5:403465/137‑1 (MQ=2)
gAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCCATCAAAAATTTATGAAAAAAAGCTGTACGGTGCAGCGGGGTGGAGCCGCCTGGTAGATAGTCGGGCGGATaa > 4:916820/1‑140 (MQ=14)
caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTTGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTACAGGAAAAAAACCAGTACGCTGCCGCTGGGTTGCGCATCATTGTAGCTCGTCCGTCACATAACACGaa > 6:832681/1‑140 (MQ=2)
caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAAGCAGAACGGTGACGCGGGGTGGCGCAGCCTGGTAGCTCTTCGGGCGCATAACGCGaa > 2:742281/1‑140 (MQ=14)
caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTTATGAAAAAAAGCAGTAAGGTGAAGAGGGGTGGAGCAGAGTTGTAGGTAGTCGGGCTCTTAACCAGaa > 2:368766/1‑140 (MQ=11)
tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACg < 6:647954/89‑1 (MQ=38)
tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACg > 5:647954/1‑89 (MQ=38)
tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGa < 8:442150/93‑1 (MQ=37)
tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGATAGAAAGCAGTACGGTGCCGCGGGGTGGAGCAGCATGGTTGATGGGCGGGCGCATACCCCGAAGgtcg > 3:904685/1‑140 (MQ=11)
gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAAATTAGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGGTGGTCGGGCGCATCACCGGACGgtgg > 7:931613/1‑136 (MQ=11)
cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAAGAAAGCAGTACGGTGACGAGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCACATAACCCGAAGGgcgtcg > 3:501491/1‑140 (MQ=11)
cgtcgGTCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCACGCAACCAATAAAAATATTATGAAATAAAGCAGTAAGGAGACGCTGGGAGGAGCAGACTGGTAGCTCCTCGGGCGAATAACCAGAACGtcgtcgcg > 4:303898/1‑138 (MQ=11)
cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCAATCAAATTTAGATGAAATAAAGCAGTACGGTGCCGCGGGGTGGGGCAGCCTGGTTGCGAGTGGGGCTCGTCACCCGAATGTCGTCGGt > 1:64180/1‑140 (MQ=11)
cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAATATTTTATGATATAAAGCAGTACGGTGCGGAGGGGTGGAGCTGCGTGGTAGCTGGTCGGGCTCAGACCCCGACGGTGGTGGGt > 3:234008/1‑140 (MQ=11)
cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg < 2:708987/87‑1 (MQ=38)
cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag < 2:135498/97‑1 (MQ=37)
cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag < 2:82690/97‑1 (MQ=37)
cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGTAAAAAAGCAGTACGGTGACGAGGGGAGGAGCTGCCTGGTAGCTCGGGGGGCTCATAACCCGAAGGTCGTGGGt > 5:857212/1‑140 (MQ=11)
tcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTTATGATATAAAGCAGTACGCTGAATCGGGGTGGAGCAGAATTCTAGATCCTCGTTCTCATAACCCGAATgtagtactt > 8:347510/1‑134 (MQ=11)
tcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAAGCAGAAAGGTGACGCGGGGTGGAGCAGCGTGGTAGATAGTCGGGCGCATAACCCGAATGTCGTCGGtta > 3:22975/1‑139 (MQ=11)
aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGCCGCGGGGTTGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCAGAATGTCGTCGGTTCAACTCAGGcccccccc > 1:464857/1‑137 (MQ=11)
aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTgg < 8:405313/77‑1 (MQ=39)
aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTAGAGGAAATAAACCTGTACGGTGACGCTGGCTGGAGGAGCCTGGTAGCTAGTCGGGCTGATAACCCTAAGGTCGTCGGTTCTAATCCGGCCACCGcc > 1:425462/1‑139 (MQ=11)
tcgtcgGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGTAAGAAAGCAGTACGGTGACGCGGGGTGGAGCTGCCTGGTAGCTAGTCGTGCTCATCCCCCGAAGGTCGTCGGGTCGAAACCGGCCCCCGCAAc > 7:160096/1‑138 (MQ=11)
gtcgGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAAGCAGTACGGTGACTCGGGGTGGAGCAGACTGGTAGCTAGGCGGTCTAATAACCCGAAGGTCGTCGGTGCAACTAAGGCCCCCGCCACAAAt > 4:93295/1‑140 (MQ=11)
gCCCCCGCAACCAATCTAAATTTGATGAAATAAAGCTGTACGGTGCCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCGGAAGGTCGTCGGGTCAACTCCGGCCCCCGCCACCATTTTAGTAGatgtctgc > 6:964116/1‑133 (MQ=14)
gCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACTCGGGGTGGCGCAGCCTTGTAGATAGTCGGGCTCTAAACCCGAAGGTCGTCGCTTCAAATCCGGACCCCGCAACCAATATAATAGAATTCtta > 4:530267/1‑138 (MQ=255)
cccaGCAACCAATAACATTTAGTTGAATTTAAGGACTACCGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACa < 1:135749/140‑1 (MQ=14)
cGCAACCAATCAAAATTTGATGAAATAAAGCTCTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCGCAAAACCAGAAGGTCGTCGGTTCAAAAACGGCGACCGCAAACAATTTATTAGAATGCTGCCAAAc > 2:577570/1‑140 (MQ=14)
aCCAAACATAATTTTGTGTAATTAACCAGTCCGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCa < 1:810815/140‑1 (MQ=17)
aaTTAAAATTTGATGAATTCACCCATTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAg < 1:796764/140‑1 (MQ=17)
aaTCATAATTTGAAGTAATTAAGCATTACGGTGACGCGGGGTGGAGCAGCCTGGGAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAg < 3:900781/140‑1 (MQ=18)
aTCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGACGGTTCAAATCCGGCCCCCGCAGCCAATTTATTAAAATGCTGACAAACCGCAAAgc > 4:549007/1‑140 (MQ=255)
cAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTC‑GGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAgct > 7:427268/1‑138 (MQ=255)
gatgaAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATTCTGACAAACCGCAAAGCTGCTATTGGc > 5:990386/1‑140 (MQ=255)
atgaAATAAAGCAGGACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGcg > 1:512070/1‑139 (MQ=255)
|
CGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCT > NZ_CP022686/1546971‑1547239
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
