Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F11 I0 R1 249 11230.8 10274356 98.4% 10109966 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,547,110 G→C 18.2% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,547,1100GC18.2% 123.0 / 6.5 76intergenic (+22/‑12)CIW80_RS07710/CIW80_RS07715tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (43/18);  new base C (13/2);  total (56/20)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCT  >  NZ_CP022686/1546971‑1547239
                                                                                                                                           |                                                                                                                                  
ctcAACCAAATATACTTTGATGTAGTTTAGCCGTACGCGGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  8:359131/138‑1 (MQ=14)
cgaacacAATTAATAATTCATGAAGTAAAGCCTTAAGGTGTCGCGTGGTGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  7:695237/134‑1 (MQ=14)
cGCAACCTATTAAAATTTGATGAAGTCACGCCGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCGCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  6:584874/140‑1 (MQ=17)
cGCAACCAATTAAATTTAGTTGAAGTAAAGGCGTCCGGGGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  7:240640/140‑1 (MQ=17)
cGCAACCAATTAAACTTTGATGAAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  6:692371/140‑1 (MQ=255)
cGCAACCAATTAAAATTTGTTGTAGTAAAGCAGTACTGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  5:192597/140‑1 (MQ=255)
cGCAACCAATTAAAATTTGTTGAATTTACGCAGTACGGTGACGCGGGGGGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  2:618359/140‑1 (MQ=17)
cGCAAACAATTATATTTTTATGAAGTAATGCAGTACGGGGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  2:1234121/140‑1 (MQ=17)
cGCAAACAATAATAATTTTATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  2:1122980/140‑1 (MQ=255)
  caaagaaTAAAATTTTGTTGTAGTAATCCAGTACGCTGTCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAACTCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAg                                                                                                                                    <  4:202327/133‑1 (MQ=14)
   atcctaTTAAATTTTGAAGTATTATACGATTACTGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCTGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAg                                                                                                                                 <  6:633872/135‑1 (MQ=14)
             aaTTTTCTGAAGTCTAGCAGTACTGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGTTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg                                                                                                                       <  7:1073278/140‑1 (MQ=14)
             aaTTTGATGAATTAATGCAGTTCGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGGAGGTCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGAcg                                                                                                                       <  1:1053667/140‑1 (MQ=17)
                  cttgaAGTAACTCATTACGCTGACGCTGGGTGGAGCAGCCTGGTCTCTCGGCGGGCTCATAACCCGAAGGTCGGCGGTGCTAATCCCGCCCCCGCAACCAATCAAACTTTGTTGAAATAACGCAGTACGGTGACGCgggg                                                                                                                  <  6:387672/138‑1 (MQ=11)
                   atgaAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAATAATTAATCTAAAAAAGCAGTACGGTGaatctgg                                                                                                                   >  4:1047067/1‑132 (MQ=14)
                       tgTATAGCATTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTgg                                                                                                               <  4:439416/137‑1 (MQ=255)
                         tAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAACAATGAATATTTTATGAAAAAAACCAGAACGGTGACTCGGGGttgcg                                                                                                             >  4:704905/1‑134 (MQ=14)
                              cattACGGAGACGCGGGGGGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                        <  4:425429/135‑1 (MQ=255)
                                gTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAAGCAGTACGGTGACGCGGGGTGGCGCTGCATGGt                                                                                                    >  1:283586/1‑140 (MQ=255)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGTAAATTTGTTGAAACAAAGCACTACGGTGCCTGGGGGTGgaggagggtgggt                                                                                                   >  5:1142368/1‑133 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAAATTAGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa                                                                                                   >  7:300458/1‑140 (MQ=255)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTAAATTTGTTGATAAAAAGCTCTACGGTGCAACTGGGTGGAGCAGCATGGtt                                                                                                   >  1:522890/1‑139 (MQ=14)
                                 tACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATATAAATTAGAGGAGAAAAACCAGAACGCTGCCTGGGGGTGGCGCAGACTgggt                                                                                                   >  8:580659/1‑138 (MQ=14)
                                   cGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCTAAAATTGATCAAAAAAACCGCTACGGTGCCTAGGGGTGGAGCATACTTGTAGc                                                                                                 >  2:530417/1‑140 (MQ=14)
                                   cGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCTGGGTGGAGCAGCCTGGTatg                                                                                                 >  7:202804/1‑138 (MQ=255)
                                      tGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAAATTGATGAAAAAAAGCAGTACGGTGCAGAGGGGTGGAGCAGCGTTGTAGCtcg                                                                                              >  1:915369/1‑140 (MQ=11)
                                      tGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATATTTAAGCAAAAAAACCACAAAGCTGCCACTGGCTGGAGCAGCATGGTAGCtat                                                                                              >  2:890428/1‑138 (MQ=11)
                                      tGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTAGATGAAATAAACCAGTACGCTGACGATGGGTGGAGCAGCATTGTAgatag                                                                                              >  1:830323/1‑136 (MQ=11)
                                        aCGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATATTGATGAAATAAAGCAGTACGGTGACTCGGGGTGGAGCAGCGTTGTTGCtgg                                                                                              >  3:775291/1‑136 (MQ=11)
                                          gatttgTGGAGCAGCTTTGTAGCTCTTCGGGCTCATAACCCGAAGGCCGTAGGTGCAAATACTGCCCGCTCAACCAATAATAATTTGATGAAATAAAGCAGTACTGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg                                                                                          <  8:58187/135‑1 (MQ=11)
                                           cgGGGTGGAGCAGCCTGGTAGCTCGGAGGGCTCATAACCCGAAGGTCGGCGGGTCATATCCGGCCCCCGCAACCAATCAAAATTAGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                           <  3:860117/138‑1 (MQ=11)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGTAAATTTGATGAAATAAACCAGTACGGTGAAGCGGGGTGGCGGAGGCTGGTTGATAGTCggt                                                                                         >  8:901789/1‑139 (MQ=11)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATATAATTTAGATCAAAAAAACCTGTACGGTGCCGCGGGTTGGAGCGGCGTGGTAGCTAGACggg                                                                                         >  5:1196505/1‑140 (MQ=11)
                                           cgGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATATTTGATGATAAAAAGCAGTAAGGTGAGGATGGGTGGCGCTGCCTGGTAGCTAGTCggg                                                                                         >  1:33424/1‑140 (MQ=11)
                                                        caTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATATAAATTAGATGAAAAAAAGCTGAACGGTGCAGAGGGGTGGAGCAGCCTGGTAGCTATTCGGGCACTTAAccagta                                                                            >  5:144508/1‑136 (MQ=11)
                                                         aTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATACGGCCCCCGCAACCACTAAAAATTTGAGGAAAAAAAGCAGTACGGTGCCTAGGGGTGGAGCTGCCTGGTAGATCGTCGGGCTGATAACCC‑gc                                                                            >  3:1221182/1‑137 (MQ=11)
                                                          tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGATATAAAGCAGTACGGTGCCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCGGATACCCC‑GAAgg                                                                         >  2:592783/1‑140 (MQ=11)
                                                          tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAAGCAGTACGGTGCCGAGGGGTGGAGAAGACTGGTAGCTCGACGGGCTCAGAACCC‑Taa                                                                           >  4:1156262/1‑138 (MQ=11)
                                                          tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGAGGAAAAAAAGCAGTACGGTGAAGCTGTGTGGAGCAGCCTGGTAGCTAGTCGTGCTCATAACCA‑Gaa                                                                           >  4:1162216/1‑138 (MQ=11)
                                                          tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTACAAATCCGGCCCCCGCAACCAATCAAAAATTGATGAAAAAAAGCAGTACGGTGCCGAGGGCTGGAGCAGCCTTGTAGCTCCACGGGCTCATACCCC‑Gac                                                                           >  4:659764/1‑137 (MQ=11)
                                                          tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCAACCAATCAAATTTTGATGAGATAAAGCAGTACGGTGCCGCGGGGTGGAGCAGCGTGGGAGATCGTCGGGCTCTTCACCA‑GAAgg                                                                         >  6:530000/1‑140 (MQ=11)
                                                             tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                             <  1:525249/102‑1 (MQ=35)
                                                             tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                             >  2:525249/1‑102 (MQ=35)
                                                             tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAAATTGATGATAAAAACCACTAAGGTGAAGAGGGGTGGAGCAGACTGGTAGCTAGTCGGGCACAAAACCA‑GAACgtcc                                                                      >  5:970934/1‑139 (MQ=11)
                                                              tgCTCGTGGGGCTCACAAGCCGAAGGTCGGCGGTGCCAATCCCGCCCCCGCAACCAATCATACATTGTAGAAATAAAGGCGTCCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGgtcgt                                                                     <  7:1131109/139‑1 (MQ=11)
                                                              agatcggcgGGCTAATAACCCGAAGTGCGTCTGTGCATATCCGGACCCCGCAACCAATCAAAATTTCATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGgtcgt                                                                     <  1:1194334/137‑1 (MQ=11)
                                                               gCTCGTCGGGCTCATACCCCGAAGGTCGTCGGTTCAACTCCGGCCCCCGCAACCAATCAAAATTTTAGGAAAAAAAGCAGTACGGTGAAGCTGGGTTGAGCTGCCTGGTTGCTAGTCGGGCTCATAACCA‑GTAGgtcg                                                                      >  3:1025829/1‑138 (MQ=11)
                                                               gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAAATTGATGAAATAAAGCAGTCCGGTGCCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGTGCGCATAACCC‑GAAGgtcg                                                                      >  3:849445/1‑138 (MQ=11)
                                                               gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATATTTGATGTAATAAACCAGTACGGTGCCTCTGGGTGGAGGAGGGTGGTTGGTAGTCGTGCTCATAACCA‑GAAGgtcg                                                                      >  3:489230/1‑138 (MQ=11)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAATATTTTATGATAAAAACCACTACGGTGACGAGGGGTGGAGCAGCCTGGTAGCTCTTCGGGCGAATCACGC‑GTAGGTGGTCGGt                                                                 >  8:1013930/1‑140 (MQ=11)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCgggg                                                                                                                  <  7:247724/92‑1 (MQ=37)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCgggg                                                                                                                  >  8:247724/1‑92 (MQ=37)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAAAAAACCAGTACGGTGACGCTGGGTGGAGCAGCCTGGTAGATAGACCGTCAC‑TAACCCAGAAAGTCGTCGGt                                                                 >  2:236450/1‑140 (MQ=11)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAATATTTGATGAAATAAACCGGTAAGGTGAATCGGGGTTGAGCAGACTGCGTGCTCGTCGGTCTCTTAACCC‑GAAGgtcgtgcgt                                                                 >  2:951588/1‑136 (MQ=11)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATCAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATACCCC‑GAAGGTCGTCGGt                                                                 >  1:1331395/1‑140 (MQ=255)
                                                                   gtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTAGATGAAAAAAAGCAGTACGGTGACGAGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCATCGGtt                                                                >  5:818980/1‑140 (MQ=255)
                                                                     cgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgt                                                                                             >  1:625261/1‑110 (MQ=40)
                                                                     cgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgt                                                                                             <  2:625261/110‑1 (MQ=40)
                                                                       ggCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCTGCCTGGTAGCTCGACGGGCTCATAACCC‑GAAGGTCGTCGGTTCa                                                              >  3:653773/1‑138 (MQ=255)
                                                                                    aaGGGCGTCGGTTCAAATCACGCCCCCGCAACCAATCACACATTGCAGAATTCAACCACTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCa                                               <  1:1123804/140‑1 (MQ=11)
                                                                                      ggTCGTCTGTGCTAATCCTGACCTATCAACGAATAAAAATTTTCTGAAATATAGCACTACGGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCa                                               <  3:606638/138‑1 (MQ=11)
                                                                                      ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAAATAGATGAGAGAAAGCAGTCCGCTGACGCTGGGTGGAGCCGCCTTGTAGGTCGTCGGGCGCATCACCC‑GAAGGTACTCGGTTCGACTCCGGGCACCGCAAc                                             >  2:861024/1‑140 (MQ=2)
                                                                                           tcgGTTCAAATCCGGCCCCCGCAACCAATCAAAAATTGTTGAAATAAACCAGAACGGTGACGCGGGGTTGAGCATAATGGTAGCTAGACCGTCGATTAACCA‑GAAAGTCGTCCGTGCAAATACGGCCCAAGCAAACaaat                                        >  8:306265/1‑138 (MQ=2)
                                                                                            cgGTTCAAATCCGGCCCCCGCAACCAATGAAATTTTGATGAAATAAAGCAGTACGGTGAAGCGGTGTGGAGCAGACTGGTAGCTCGTCGGGCTCATAACCA‑GAATGTCGTCGGTGGAAATCACGCAACCGCAAACAAt                                         >  4:877861/1‑138 (MQ=11)
                                                                                            cgGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATGAAATAAAGCAGTACGGTGCCTCGGGGTGGCGCAGCCTGGGAGCTAGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATACGGCCCCCGCAGCCAAt                                         >  3:805657/1‑138 (MQ=11)
                                                                                                         gCCCCCGCACCCAATCTAAAATTGATGAAATAAAGCATTACGGAGACGCGGGGTGGAGCTGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAATGTCGTCGGTTCAAATCCGGACCCAGCAACCAATTTATTAGAATGCTGa                          >  1:1070130/1‑140 (MQ=17)
                                                                                                         gCCCCCGCAACCAATCTAAATTTGATGAAATAAAGCACTAAGGTGACGCGGGCTGGAGCAGACTGGTAGCTCGTCGTGCTAATAACCC‑GAAGGTCGTCGGTTCAACACAGGCACCCGCAAACAAATTATTAGAAAGATGa                          >  1:651699/1‑140 (MQ=14)
                                                                                                         gCCCCCGCAACCAATCAAATTTTGAGGAAATAAAGCAGTACGGTGACGCGGGGTGGCGCAGCATGGTAGCTCGACGGGCGCATAACCA‑GAAGGTCGTCGGTTCAACTCCGGCCCCCGCGACCAATTTATTAGAATGCTGa                          >  2:527350/1‑140 (MQ=14)
                                                                                                         gCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCAGCCACCCGCACCCAATTTATTAGAATGCTgc                          >  7:175893/1‑139 (MQ=255)
                                                                                                         gCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTGCCAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGa                          >  5:61450/1‑140 (MQ=255)
                                                                                                         gCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGAGGGGTGGAGCAGCCTGGTAGCTAGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCAGGCCCCCGCAACCAATTAATTAGAATGCTGa                          >  2:1290001/1‑140 (MQ=255)
                                                                                                         gCCCCCGCAACCAATAAAAATTTGATGAAATAAAGCAGTACGGTGCCGCGGGGTGGCGCAGCATGGTAGCTCGTCGGTCTAATAACCC‑GAACGTAGAGGGTTAAAAACAGGCACCCGCAACAAATTTAATAGATTTCTGa                          >  6:1228677/1‑140 (MQ=2)
                                                                                                         gCCCCCGCAACCAATAAAAATTTGATGAAATAAAGCAGTACGGTGCCGAGGGGTGGCGCAGCCTGGTAGATAGTAGGGCTAATAACCC‑GAAGGTCGTCGGTGCAACTCCGTCCCACGCAACCAATTTAATACAAtgatga                          >  8:1146340/1‑140 (MQ=14)
                                                                                                           ccccGCAACCAATCAAAATTTGATGAAATAAAGAAGTACGGTGACGCGGGGTGGAGCTGCCTGGTAGCTCGTCTGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGa                          <  3:877861/138‑1 (MQ=255)
                                                                                                              cGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAAc                     >  8:464967/1‑140 (MQ=255)
                                                                                                              cGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGAGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCAGACAAAc                     >  2:7942/1‑140 (MQ=255)
                                                                                                                 aaCCAATCAAATAGTGTAGAAATAACCGAGTACGCTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGc                  <  7:400448/140‑1 (MQ=17)
                                                                                                                     actataaaTTTGATGTATAAAGGCAGTACTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAg              <  7:62578/135‑1 (MQ=17)
                                                                                                                      attaaaaATTGAAGAAATAAAGCAGTACGGTCACTCGGGGTGGAGCAGCCTGGTAGCTCGTCTGGCTCAAAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAgc             <  1:330340/137‑1 (MQ=17)
                                                                                                                        cAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCTAATCCGGCCCCCGCAACCAATTTATTAGAATTCTGACAAACCGCAACgc             >  4:396434/1‑138 (MQ=255)
                                                                                                                         aaaaTTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAACTCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAgct            >  4:1131782/1‑138 (MQ=255)
                                                                                                                         aaaaTTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAgctgc          >  2:398804/1‑140 (MQ=255)
                                                                                                                           aaTTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTa        >  1:1204188/1‑140 (MQ=255)
                                                                                                                            aTTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTAt       <  2:1204188/140‑1 (MQ=255)
                                                                                                                               tgatgaAATAAAGCAGTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCA‑GAAGGACGTCGGTGCACCTCACGCCCCCGCAACCTAGTTATTAGAATGCTGACATACCGCAAAGCTCATATTgg    >  1:592843/1‑140 (MQ=17)
                                                                                                                                 atgaAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCt  >  5:1005597/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                  
CGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCATGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAATTTGATGAAATAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCC‑GAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTTATTAGAATGCTGACAAACCGCAAAGCTGCTATTGGCT  >  NZ_CP022686/1546971‑1547239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A